Product Datasheet. Ago2/eIF2C2 Antibody (2E12-1C9) H M01. Unit Size: 0.1 mg. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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1 Product Datasheet Ago2/eIF2C2 Antibody (2E12-1C9) H M01 Unit Size: 0.1 mg Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 1 Publications: 59 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 4/26/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 H M01 Ago2/eIF2C2 Antibody (2E12-1C9) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity 0.1 mg Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Monoclonal 2E12-1C9 No Preservative IgG1 Kappa IgG purified Buffer PBS, ph 7.4 Product Description Host Mouse Gene ID Gene Symbol Species Reactivity Notes EIF2C2 Human, Mouse, Rat Rat reactivity reported in scientific literature (PMID: ). Mouse reactivity reported in scientific literature (PMID: ) Specificity/Sensitivity EIF2C2 - eukaryotic translation initiation factor 2C, 2 Immunogen Notes Product Application Details Applications EIF2C2 (AAH , 1 a.a. ~ 377 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. This product is produced by and distributed for Abnova, a company based in Taiwan. Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, RNA Inhibition Recommended Dilutions Western Blot 1:500, Chromatin Immunoprecipitation 1:10-1:500, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation, Immunohistochemistry-Paraffin 1:10-1:500, RNA Inhibition Application Notes Page 1 of 5 v.20.1 Updated 4/26/2018 Antibody reactive against recombinant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), RNAi validation and ELISA and Sandwich ELISA. Chromatin Immunoprecipitation was reported in scientific literature. Use in immunprecipitation reported in scientific literature (PMID )

3 Images Marker gene expression based FACS-sorted NIH3T3 cells lysed in RIPA Buffer and resolved on 10% SDS PAGE. Image from verified customer review. Page 2 of 5 v.20.1 Updated 4/26/2018 Immunocytochemistry/Immunofluorescence: Ago2/eIF2C2 Antibody (2E12-1C9) [H M01] - Analysis of monoclonal antibody to EIF2C2 on HeLa cell. Antibody concentration 10 ug/ml. Immunohistochemistry-Paraffin: Ago2/eIF2C2 Antibody (2E12-1C9) [H M01] - Analysis of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml. Analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi ( Cat # H R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # H M01 ). GAPDH ( 36.1 kda ) used as specificity and loading control.

4 Analysis of EIF2C2 expression in transfected 293T cell line by EIF2C2 monoclonal antibody (M01), clone 2E12-1C9. Lane 1: EIF2C2 transfected lysate (Predicted MW: 42.4 KDa). Lane 2: Non-transfected lysate. Page 3 of 5 v.20.1 Updated 4/26/2018 Analysis of EIF2C2 expression in MCF-7. ELISA: Ago2/eIF2C2 Antibody (2E12-1C9) [H M01] - Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.

5 Publications Daga N, Eicher S, Kannan A et al. Growth-restricting effects of sirna transfections: a largely deterministic combination of off-target binding and hybridization-independent competition. Nucleic Acids Res. Sep :00AM [PMID: ] (Human) Ghosh S, Bose M, Ray A et al. Polysome arrest restricts mirna turnover by preventing exosomal export of mirna in growth-retarded mammalian cells. Mol Biol Cell Mar 15 [PMID: ] (WB, Human) Yuan Y, Kluiver J, Koerts J et al. mir-24-3p Is Overexpressed in Hodgkin Lymphoma and Protects Hodgkin and Reed-Sternberg Cells from Apoptosis. Am J Pathol 2017 Apr 19 [PMID: ] Curtale G, Renzi TA, Drufuca L et al. Glucocorticoids downregulate TLR4 signaling activity via its direct targeting by mir-511-5p. Eur J Immunol 2017 Aug 4 [PMID: ] Huang JH, Ku WC, Chen YC et al. Dual mechanisms regulate the nucleocytoplasmic localization of human DDX6. Sci Rep 2017 Feb 20 [PMID: ] Elkayam E, Faehnle CR, Morales M et al. Multivalent Recruitment of Human Argonaute by GW182. Mol Cell 2017 Aug 3 [PMID: ] Bose M, Barman B, Goswami A, Bhattacharyya SN. Spatio-temporal uncoupling of mirna-mediated translational repression and target RNA degradation controls mirnp recycling in mammalian cells. Mol Cell Biol 2016 Nov 28 [PMID: ] Wang WT, Ye H, Wei PP et al. LncRNAs H19 and HULC, activated by oxidative stress, promote cell migration and invasion in cholangiocarcinoma through a cerna manner. J Hematol Oncol 2016 Nov 3 [PMID: ] Mantel PY, Hjelmqvist D, Walch M et al. Infected erythrocyte-derived extracellular vesicles alter vascular function via regulatory Ago2-miRNA complexes in malaria. Nat Commun 2016 Oct 10 [PMID: ] Zhao Y, Yin Z, Li H et al. MiR-30c protects diabetic nephropathy by suppressing epithelial-to-mesenchymal transition in db/db mice Aging Cell Jan :00AM [PMID: ] (IP, Mouse) He M. mirna Tagging and Affinity-purification (mirap). Bio Protoc 2012 Oct 5 [PMID: ] Zeng Y, Sankala H, Zhang X, Graves PR Phosphorylation of Argonaute 2 at serine-387 facilitates its localization to processing bodies. Biochem J 2008 Aug 1 [PMID: ] (WB) More publications at Page 4 of 5 v.20.1 Updated 4/26/2018

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to H M01 NB HAF007 NB720-B NBP mg Human Bladder Whole Tissue Lysate (Adult Whole Normal) Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG1 Kappa Light Chain Isotype Control (P ) Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: