Product Datasheet. rrna Antibody (Y10b) NB Unit Size: 0.1 ml. Store at 4C. Do not freeze. Reviews: 1 Publications: 16

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1 Product Datasheet rrna Antibody (Y10b) NB Unit Size: 0.1 ml Store at 4C. Do not freeze. Reviews: 1 Publications: 16 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/11/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB rrna Antibody (Y10b) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer 0.1 ml 1.0 mg/ml Store at 4C. Do not freeze. Monoclonal Y10b 0.02% Sodium Azide IgG3 Kappa Protein A purified PBS Page 1 of 6 v.20.1 Updated 2/11/2019 Product Description Host Species Reactivity Notes Specificity/Sensitivity Immunogen Product Application Details Applications Recommended Dilutions Application Notes Mouse Human, Mouse, All Species This antibody reacts with all eukaryotes. This is specific for ribosomal RNA. There is no cross-reactivity with other RNAs. The whole 5.8S ribosomal RNA. Flow Cytometry, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:2500, Immunoprecipitation 10 ul/lysate, Immunohistochemistry-Paraffin, Flow (Intracellular) This rrna Antibody (Y10b) is useful for Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In ICC/IF, staining was observed in Ribosomes in the nucleoli and cytoplasm of Ntera2 cells. Images Immunocytochemistry/Immunofluorescence: rrna Antibody (Y10b) [NB ] - HeLa cells were fixed and permeabilized for 10 minutes using -20C MeOH. The cells were incubated with anti-rrna (Y10b) conjugated to DyLight 488 [NB G] at 5ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

3 Immunohistochemistry: rrna Antibody (Y10b) [NB ] - Immunohistochemical staining of mouse neuronal progenitor culture using rrna antibody (green). Image from verified customer review. Page 2 of 6 v.20.1 Updated 2/11/2019 Flow (Intracellular): rrna Antibody (Y10b) [NB ] - An intracellular stain was performed on A549 cells with NB AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/ml for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Immunocytochemistry/Immunofluorescence: rrna Antibody (Y10b) [NB ] - rrna antibody was tested in Ntera-2 cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red). Immunocytochemistry/Immunofluorescence: rrna Antibody (Y10b) [NB ] - Staining of rrna in vero cells.

4 Flow (Intracellular): rrna Antibody (Y10b) [NB ] - Analysis using Alexa Fluor (R) 488 conjugate of NB An intracellular stain was performed on HeLa cells with rrna antibody (Y10b) NB (blue) and an isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/ml for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Page 3 of 6 v.20.1 Updated 2/11/2019 Flow (Intracellular): rrna Antibody (Y10b) [NB ] - Analysis of Alexa Fluor (R) 647 conjugate of NB An intracellular stain was performed on HeLa cells with rrna (Y10b) antibody NB AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% Flow (Intracellular): rrna Antibody (Y10b) [NB ] - An intracellular stain was performed on RAW cells with rrna Antibody (Y10b) NB APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/ml for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.

5 Publications Tsalikis J, Tattoli I, Ling A et al. Intracellular bacterial pathogens trigger the formation of U bodies through metabolic stress induction J. Biol. Chem Jul 01 [PMID: ] Mastroeni D, Grover A, Delvaux E et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 2010 Dec [PMID: ] (Human) Fischer S, Gesierich S, Griemert B et al. Extracellular RNA liberates Tumor-Necrosis-Factor-alpha to promote tumor cell trafficking and progression. Cancer Res 2013 Jun 17 [PMID: ] (IHC-P, ICC/IF, Human) 3) Bleher, R, Martin, R. Ribosomes in the squid giant axon. Neuroscience;107(3): [PMID: ] (ICC/IF, Plant, Other) 9) Thomas, MG, Martinez Tosar, LJ, Loschi, M, Pasquini, JM, Correale, J, Kindler, S, Boccaccio, GL. Staufen recruitment into stress granules does not affect early mrna transport in oligodendrocytes. Mol Biol Cell;16(1): Jan. [PMID: ] 7) Sotelo-Silveira, JR, Calliari, A, Cardenas, M, Koenig, E, Sotelo, JR. Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons. J Neurobiol;60(2): Aug. [PMID: ] (ICC/IF, Fish) 2) Anderson, KD, Merhege, MA, Morin, M, Bolognani, F, Perrone-Bizzozero, NI. Increased expression and localization of the RNA-binding protein HuD and GAP-43 mrna to cytoplasmic granules in DRG neurons during nerve regeneration. Exp Neurol;183(1): Sep. [PMID: ] 1) Aakalu, G, Smith, WB, Nguyen, N, Jiang, C, Schuman, EM. Dynamic visualization of local protein synthesis in hippocampal neurons. Neuron;30(2): May. [PMID: ] (ICC/IF, Rat) 13) Garden, GA et al. Protein masking of a ribosomal RNA epitope is an early event in afferent deprivation-induced neuronal death. Mol Cell Neurosci;6(3): Jun. [PMID: ] (IHC, Chicken) 11) Lerner, EA, Lerner, MR, Janeway, CA, Steitz, JA. Monoclonal antibodies to nucleic acid-containing cellular constituents: probes for molecular biology and autoimmune disease. Proc Natl Acad Sci U S A;78(5): May. [PMID: ] (IP, Mouse) 6) Jablonka, S, Wiese, S, Sendtner, M. Axonal defects in mouse models of motoneuron disease. J Neurobiol;58 (2): Feb 5. [PMID: ] (ICC/IF, Mouse) 10) Zheng, J, Kelly, TK, Chang, B, Ryazantsev, S, Rajasekaran, AK, Martin, KC, Twiss, JL. A functional role for intraaxonal protein synthesis during axonal regeneration from adult sensory neurons. J Neurosci;21(23): Dec 1. [PMID: ] (ICC/IF, IP, Rat) More publications at Page 4 of 6 v.20.1 Updated 2/11/2019

6 Procedures Immunocytochemistry Protocol Specific for rrna Antibody (Y10b) (NB ) Immunocytochemistry Protocol Culture cells to appropriate density in 35 mm culture dishes or 6-well plates. Page 5 of 6 v.20.1 Updated 2/11/ Remove culture medium and add 10% formalin to the dish. Fix at room temperature for 30 minutes. 2. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes. 3. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes. 4. To block nonspecific antibody binding incubate in 10% normal goat serum from 1 hour to overnight at room temperature. 5. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature. 6. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes. 7. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature. 8. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes. 9. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing. *The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.

7 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG3 Isotype Control (MG3K) NB AF488 rrna Antibody (Y10b) [Alexa Fluor 488] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: