Smiths Detection. Detection and Identification of Biological Organisms. Institute of Bio-Sensing Technology Launch Event. Tuesday 18 th March 2008

Transcription

1 Smiths Detection Detection and Identification of Biological Organisms Institute of Bio-Sensing Technology Launch Event Tuesday 18 th March 2008 Presentation by: Jason Betley, Bio Product Development Manager, SD Watford

2 Contents 1. The challenges of biodetection 2. A generic biosensor 3. Specific types of biosensor products developed by Smiths for non-laboratory use

3 1. The Challenges of Biological Detection Comparative lethality of toxic biological agents and chemical agents: the dose required to theoretically kill 9 million people. Botulinum VX Sarin Mustard 2.6 gram 24 kg 2.8 tons 87 tons Source: Adapted from Swedish Defence Research Agency FOI briefing book on Chemical Weapons threat, effects and protection No.2, 2002

4 1. The Challenges of Biological Detection Medical intervention response times for BWAs Toxins Botulism Rickettsiae, Chlamydiae Typhus Q fever Batrachotoxin Saxitoxin Ricin Aflatoxin Anthrax Plague Cholera Tularemia Glanders Brucellosis Typhoid Bacteria 10 min 20 min 40 min 1 hr 2 hr 4 hr 8 hr 1 day 2 days 4 days 8 days 16 days Time T=0 first pathogen reaches persons Viruses Encephalitis (various) Rift Valley Yellow Smallpox Ebola/Marburg

5 1. The Challenges of Biological Detection Quality of Information Gained Quantifiable, Validated QSR/ISO13485 Identifiers It s anthrax, avian flu 5-60 mins Classifiers It s a toxin, virus, bacteria s Triggers There s something In the air - seconds Time

6 2. A Generic Biosensor System Sample Cyclones, Electrostatics Direct/Sample prep Microfluidics non-micro, or dry impact! Immuno, PCR, other Fit for purpose, rugged Collection/ Preparation Fluid Handling Sensor Environmental Control Consumables Waste Mgt Power Control Size, cost, storage SIBCRA H&S Budget suitable to scenario Software Firmware Data Ouput HCI

7 2. A Generic Biosensor System What do we want? Specificity Selectivity Sensitivity Dynamic range Time to detect False positives and negatives

8 Giving technology the Smiths treatment Taking laboratory science into the field Example: Fourier Transform Infra Red (FTIR) Lab: FTIR Microscope Field: FTIR Chemical identifier for first responders

9 3. Specific Products Smart BioSensor (SBS) Rapid Detector/Classifier

10 Smart Biosensor (SBS) Fast Response Classifier (2 minutes) Small Light Low Power Dry air intake (does not require Sample Collection System) Detects broad threat classes Includes detection of unknown or GM organisms 30 cm 15 cm 13 cm Dry air intake for fast classification

11 Smart Biosensor (SBS) How does it work? Controls and blanks reduce false alarms

12 Smart Biosensor (SBS) Trigger vs SBS Conventional Triggers - Particle Counts - Biological/Non-Biological - Shape, size - Charge Smiths Smart Biosensor - Detects and Classifies bioagents - Near real time detection - Immune to ambient biologicals - Reduced cost/no. of confirmatory assays - Faster Analysis/Limit ID assays needed - Improved decision making Advantages over conventional triggers

13 Smart Biosensor (SBS) Applications Shipboard Battlefield Point Detection Vehicle Mounted First Responders SBS Variety of applications Buildings and Installations Protection

14 Smart Biosensor (SBS) Rapid Detection of Airborne Bacteria Fluorescence Intensity (Counts) Sensor responds to multiple challenges of bioagent Response time ~ 1 minute Response is quantitative Time (minutes) Quantitative response in ~ 1 minute

15 Smart Biosensor (SBS) Sensor Response to Bg after 4 hr Exposure to Paper Dust Rapid Response to 2 challenges Dust concentration particles/l Time Demonstration of Interferent Rejection

16 Smart Biosensor (SBS) Sensor Range and Sensitivity Good sensitivity quick response

17 Smart Biosensor (SBS) Interferents Tested to Date Silica powder Dust NIST Std Urban Dust Sand Fungal Spores Ragweed Pollen Dog Dander Ambient Air Salt Spray Biological Media Tonic Water Cooking Oil Window Cleaner Diesel smoke AVCAT smoke AVTUR smoke White smoke Black smoke Tobacco smoke Paper smoke Cardboard smoke SBS now production available

18 Bio-Seeq Vet PCR Identifier for Diverse Sample Types

19 Bio-Seeq Vet - Platform Overview Sample Preparation Units Reagent Packs BioSeeq Instruments Consumable product Injection moulded, wet chemical filling, ultrasonic welding assembly Common to all assays Consumable product Injection moulded cases, Contains beaded enzymes and assay specific PCR beads First products for FMDV and Avian Influenza Instrument platform for 5 simultaneous samples Durable, ruggedised and sealed to enable disinfection Portable Battery driven

20 SPU Product Description SPU is required to accomplish all of the following: Sample homogenisation/mixing with lysis/binding buffer Isolation of nucleic acids (DNA or RNA) from complex mixtures Ability to accommodate a variety of sample types Removal of PCR inhibitors Mixing of purified NA with primers, probes, enzymes Delivery of PCR-ready mixture to device Decontamination after sample introduction

21 Vehicle Systems Military Applications in the Field

22 Vehicle Systems Integrated Biological Detection System (IBDS) Chemical agent resistant material Power and water supply on board Automated sample handling Suite of connected Triggers & Identifiers Automatic sample archiving for evidence Early warning & Full identification Human Computer Interface (HCI) Fully upgradeable Remote Communications Meteorological data Biohazard Containment Suite (BHCS) Developed out of Smiths Detection Watford

23 Summary Smiths Detection Products

24 Smiths Detection Instrument Development by Smiths Detection: Proprietary Data