Supplementary Information attached to:

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1 Supplementary Information attached to: "Involvement of TrkB- and p75 NTR -signaling pathways in two contrasting forms of long-lasting synaptic plasticity" by Sakuragi, S., Tominaga-Yoshino, K. & Ogura, A.

2 Supplementary Fig. S1 a c b *** 1 d ** 3 *** 2 *** 4 Supplementary Figure S1 Confirmation of the long-lasting synaptic plasticity after repetitive inductions of LTP and LTD in the mouse slice cultures. (a, b) The stimulation protocol for the repetitive LTP-induced synaptic enhancement in the rat slice cultures 5 was applied to the mouse cultures, leading to the reproduction of equivalent enhancement. Forskolin (; 2 um, 2 min) was applied on poststimulus (PS) days, 1 and 2, and electric (b) and morphometric (b) assays were conducted on PS days (c, d) The stimulation protocol for the LTD-repetition-operated synaptic suppression in the rat slice cultures 8 was applied to the mouse cultures, leading to the reproduction of equivalent suppression. Dihydroxyphenylglycine (DHPG; um, 1 min) was applied on PS days, 1 and 2, and electric (c) and morphometric (d) assays were conducted on PS days The numbers of slices examined are 31 for both the (3 mock) and 3 groups in (a) and 28 for the (3 mock) and 34 for the 3 DHPG groups in (c). The scale bars are 1 msec (horizontal) and 1 mv (vertical). The numbers of dendritic segments [slices] examined were 83 [22] for (3 mock) and 79 [22] for 3 in (b) and 53 [11] for (3 mock) and 55 [14] for 3 DHPG in (d). The scale bars indicate 2 um. The P values are for ***1, for ***2,.58 for **3 and for ***4 (Students' t-test).

3 Supplementary Fig. S2 fepsp amp. (% of baseline) TrkB-Fc time (min) Supplementary Figure S2 Involvement of BDNF in the late phase of -induced LTP. The late-phase of the chemical LTP induced by (2 um, 2 min) is restrained by TrkB-Fc, a BDNF-scavenger (1 ug/ml, 15 min), applied immediately after the exposure to. The numbers of slices examined were 6 for and 4 for. The stimulation current delivered every 3 sec was set to the strength that evoked ~% maximal fepsp for each slice. Responses to 8 consecutive stimuli were binned and plotted. The representative recordings taken before and after the drug applications are shown. The scale bars are 1 msec (horizontal) and 1 mv (vertical).

4 Supplementary Fig. S3 a PS day b 3h +/ TrkB-Fc c *** 1 *** 2 *** 3 *** 4 Supplementary Figure S3 Inhibition of long-lasting synaptic enhancement by TrkB-Fc applied 3 hours after the third induction of LTP. TrkB-Fc, a mbdnf scavenger, was applied 1 hour after the third LTP induction as mentioned in the text and in Fig. 1d-f. From fear that a LTP of 1-hour duration was not sufficient for the development of the long-lasting synaptic enhancement, we extended the maintenance of LTP to 3 hours, resulting in equivalent results. a) Timeline of the experimental procedures. (b) The synaptic strength, as indicated by the maximal fepsp amplitude, is shown together with representative recordings. The numbers of slices examined were 18 for (3 mock), 25 for 3 and 13 for 3. The scale bars are 1 msec (horizontal) and 1 mv (vertical). (c) The dendritic is shown together with representative images of dendritic segments. The numbers of segments [slices] examined were 31 [6] for (3 mock), 47 [11] for 3, and 18 [6] for 3. The scale bar indicates 2 um. The P values are for ***1, for ***2, for ***3 and for ***4 (one-way ANOVA followed by a Tukey-Kramer multiple-comparison test).

5 Supplementary Fig. S4 kd 75 p-trk FL-TrkB Tr-TrkB TUJ1 p75 NTR anti-p75 NTR + + anti-trkb Supplementary Figure S4 A full-length immunoblotted membrane, the bands on which were taken out and depicted in text Fig. 4d Three images are taken from a single identical membrane, reacted with antibodies (indicated beside) and washed repeatedly. The treatment applied to the culture is indicated below each lane.