Surfaces with added value: Bioengineering for Bioanalysis and Medicine

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2 Surfaces with added value: Bioengineering for Bioanalysis and Medicine Hans Sigrist :: 24/09/2003 :: Page 2

3 Molecular and Thin-Film Engineering of Surfaces Material + soluble or solid matter? (adsoptive) Material functionalization (adsoptive, chemisorptive, covalent)? Change of surface physico-chemical properties Material bio/functionalization? Bioactivity? Biocompatibility Carbohydrate microarray Nanoparticle on glass Zeolite Hans Sigrist :: 24/09/2003 :: Page 3

4 Introduction Biofunctionalization for Bioanalytics Biomaterials Biotextile Biomedical devices Hans Sigrist :: 24/09/2003 :: Page 4

5 Introduction Surface CSEM Development of innovative surface chemistries Manufacturing and development of biochips, biosensor platforms and bioengineered materials Services/consulting in bioanalytics, material and textile bioengineering Technology Products Applications Hans Sigrist :: 24/09/2003 :: Page 5

6 Technology Bioengineering with Light Biomolecules (e.g. antibodies) Biomolecule mixed with photolinker polymer Photolinker polymer on flat support LIGHT? = 350 nm Hans Sigrist :: 24/09/2003 :: Page 6

7 Technology Linker Polymer X monomer monomer m n Y X, Y = functional groups: photoreagent, COO -, NH 3+, Biotin Thiol, Maleimide, Fluorophore, Oligosaccharide Monomer = glucose? OptoDex = amino acid = disaccharide? HA = organic building block Distance X to Y = 5 to 8 nm? HD = 20 to 30 nm? LD Hans Sigrist :: 24/09/2003 :: Page 7

8 Technology Carbene Chemistry? = 350 nm N N N N N N CF 3 CF 3 carbene CF 3 Si O A H CF 3 covalent immobilisation A H CF 3 Hans Sigrist :: 24/09/2003 :: Page 8

9 Technology Why Photochemistry? Do it the light Addressable? patterning, mask assisted Favorable reaction conditions for biomolecules Timed initiation and termination of reactions Chemical bond formation = statistical event Carbene philicity way Density gradients made by light (single photon chemistry?) Hans Sigrist :: 24/09/2003 :: Page 9

10 Technology Proprietary Technology and Tools Key features Covalent immobilization by light Low non-specific binding Single-step process Versatility: applicable with all materials, in all formats and detection principles A cost-effective solution for Biomolecule immobilization Surface passivation/activation Hans Sigrist :: 24/09/2003 :: Page 10

11 Technology Surface Passivation by OptoDex Surface density (ng/mm 2 ) OptoDex surface Untreated surface Brain homogenate Tear fluid Blood serum Hans Sigrist :: 24/09/2003 :: Page 11

12 Technology ONE Surface Chemistry for all.. Biomolecules any type of carrier material Proteins Enzymes Antibodies Antigens Allergens Carbohydrates Peptides Oligonucleotides Drugs Cell extracts Metabolites Amino acids on Glass Metaloxides Silicon, silicon nitride Diamond, Carbon Diamond-like carbon Polystyrene Polyuretahne Polycarbonate Polyester Parylene Polyvinylalcohol Degrapol? Polyvinyl difluoride Leads to high flexibility and admits numerous applications Hans Sigrist :: 24/09/2003 :: Page 12

13 Technology The Laboratories Cleanroom 1000 facility for PhotoChip and Functional Platform Production: plasma activation, bioprinting, detection, photobonding, packaging Production and Application Laboratory fully equipped for photolinker polymer production and assay development Hans Sigrist :: 24/09/2003 :: Page 13

14 Products arrayon Products and Offers arrayon PhotoChips arrayon Functional Platforms Photolinker polymers (e.g. OptoDex ) Customized analytic platform engineering Customized arrayon bioarrays Textile bioengineering Material passivation / engingeering Hans Sigrist :: 24/09/2003 :: Page 14

15 Products arrayon PhotoChip PhotoChip coated with photolabeled Optodex (inactivated) 2 Addition of Biomolecules immobilization by light in a one step process 350 nm one step PhotoChip Bioarray on PhotoChip Photolabel Material (glass, plastic etc.) Interlayer (Optodex ) Key Features/Advantages 1-step immobilization Applicable for any type of biomolecule Mixtures of different classes of molecules possible Designed for: large volume chip production, screening Hans Sigrist :: 24/09/2003 :: Page 15

16 Application Allergen Detection in Human Blood Serum OD 405 nm Specific MAb Non-specific MAb OD 405 nm Allergic human serum Non-allergic human serum OptoDex / Allergen OptoDex 0.0 OptoDex / Alllergen OptoDex Photobonding of allergen (birch pollen) on optical sensor surfaces. ELISA assay with photobonded probe allergens, specific monoclonal antibodies (MAb) and serum of allergic and non allergic patients. Hans Sigrist :: 24/09/2003 :: Page 16

17 Application Deciphering the Glycocode with Lectins Arrayed probes Glycoconjugate Array 2-FL-BSA asialofetuin fetuin transferrin Lectin Array SNA lectin (Sialic acid) UEA lectin (Fucose) DSA lectin (NAc Glucose ) PNA lectin (Galactose) Targets UEA Lectin (Fucose) Cy5 UEA lectin binding wih f (2-FL-BSA) Cy5-fucose (Cy5-2-FL-BSA) binding wth UEA lectin fluorescence labeled lectins target molecules fluorescence labeled glycoconjugates Hans Sigrist :: 24/09/2003 :: Page 17

18 Products arrayon Functional Platforms Optodex with various functional groups immobilized by light 2 Coupling of Biomolecules (specific for selected functional group) COOH COOH COOH COOH NH 2 NH 2 NH 2 NH 2 Photolabel (activated) Material (glass, plastic etc.) Interlayer (Optodex ) Key Features/Advantages Low non-specific binding, i.e. low background Variety of coupling chemistries available Favourable environment for biological function Designed for: Assay development, R&D Hans Sigrist :: 24/09/2003 :: Page 18

19 Pîxel Antigen array print Coupling of Proteins to OptoDex C 1-4: m-igg 5: PBS buffer 6-9: HRP-Goatanti-mIgG 10: PBS buffer 11-12: h-igg 13-14: BSA Coupled antigens are detected by scanning for immunofluorescence after complexation with antimouse IgG-Cy5 conjugate and Anti-HRP-Cy3 conjugate, respectively. (?m) Fluorescence Fluorsecence Signal (Cy5) Signal (Cy5) Signal of Of Cy3 Cy3 Signal of of Cy5 Cy m-igg HRP-Anti-mIgG h-igg BSA Background Fluorescence Fluorescence Signal Signal (Cy3) (Cy3) Fluorescence image and cross-section scan of immunostained protein spots on a Carboxy PhotoChip Hans Sigrist :: 24/09/2003 :: Page 19

20 Application Bactericidal Textile TISSUPOR wound pad Bactericidal agent Tissue background Textile treated with Lysozyme, an enzyme that suppresses bacterial prooliferation Fluorescence Signal Hour 48 Hours Bactericidal activity of bioengineered textile Hans Sigrist :: 24/09/2003 :: Page 20

21 Conclusion Focus and Uniqueness Exclusivity and markets are challenged by A unique chemistry and its versatility A technological flexibility allowing many differing applications Beneficial overall cost projections Hans Sigrist :: 24/09/2003 :: Page 21

22 T h a n k y o u f o r y o u r a t t e n t i o n.