MICROBIOLOGY 20 (3108 & 3139)

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1 Los Angeles Mission College, Spring 2009 Stephen Brown (instructor) Lab: TTh 5:25-6:50, INST 2016 (3108) Lecture: TTh 7:00-8:25, INST 2001 voic Lab: TTh 8:35-10:00, INST 2016 (3139) office hours: TTh 4:00-5:25 INST 2016 MW 5:20-6:50 Bungalow B MICROBIOLOGY 20 (3108 & 3139) PREREQUISITES: Successful completion of any of the following courses: required - Biology 3 or 6, AND Chemistry 51, 65 or 101 (or the equivalent) recommended English 28 (or the equivalent) COURSE DESCRIPTION: This course is an introduction to the fundamental principles of microbiology. The laboratory portion of the course covers microscopic and cultural techniques for studying and identifying microorganisms. COURSE OBJECTIVES: Upon completion of this course a successful student will be able to: Employ microbiology terminology correctly in the laboratory and workplace. Analyze the differences between prokaryotic and eukaryotic cells. List subcellular structures and organelles of prokaryotic and eukaryotic cells and their functions, draw the structure of prokaryotic and eukaryotic cells and label their parts. Compare and contrast different eukaryotic organisms including fungi, algae, and multicellular parasites using the compound light microscope, and describe important features. Describe the underlying mechanisms of chemical reactions in microbes, including energy and material flow. Compare and contrast the effect of environmental factors on microbial growth (ph, temperature, pressure, radiation, oxygen concentration, osmotic pressure, and nutrients). Differentiate important events of the four growth stages of bacterial populations (lag, log, stationary, and decline). Describe the processes of DNA replication, mutations, transcription, and translation. Distinguish among mechanisms of genetic recombination in bacteria (transformation, transduction, and conjugation). Organize and name the major taxonomic categories of bacteria, fungi, viruses, algae, protozoa, and helminths. Demonstrate knowledge of the different features of prokaryotes, their different taxonomical groupings and to use reference sources for microbial identifications. Analyze the traits of important microbiological eukaryotes and their clinical and environmental relevance.

2 Explain viral structure and the major steps in the life cycle of an animal virus. Describe the structure of a bacteriophage and distinguish between lytic and lysogenic life cycles. Examine and distinguish the stages of infectious disease (incubation, prodromal, illness, decline, and convalescence). Explain Koch s postulates for infectious diseases and exceptions to them. Distinguish between bacterial endotoxins and exotoxins and give examples of each. Describe microbial mechanisms to enter host, evade host defenses, and cause cellular damage. Explain the role of the nonspecific host defenses against microbial infection. Examine the features of the five categories of immunoglobulins (IgA, IgD, IgM, IgG, and IgE). Analyze specific host defenses against microbial infection, and distinguish between humoral and cell-mediated immunity. Discuss cells and cell fragments found in human blood and describe their functions in nonspecific and specific host responses. Distinguish among the major categories of immune disorders in humans, their signs and symptoms and the genetic and environmental factors that may play a role in the etiology of such diseases. Compare and contrast the major categories of antimicrobial drugs. Summarize the major diseases that affect different human organ systems due to specific microbial pathogens. STUDENT LEARNING OUTCOMES: Microbiology 20 students will: (1) Evaluate and summarize a peer-reviewed article in microbiology or immunology and write a report using proper terminology; (2) Identify an unknown bacterium using lab skills learned in the course. REQUIRED BOOKS AND MATERIALS: BOOKS: (ISBN for custom combo of books below is X) Microbiology: An Introduction. Tortora, Funke, & Case, 9 th ed Pearson-Benjamin Cummings (ISBN ) Laboratory Experiments in Microbiology. Johnson & Case, 8 th ed Pearson-Benjamin Cummings (ISBN ) MATERIALS: bound notebook (graph ruled), Sharpie pen, colored pencil set 7 Scantron 882-E forms, 10 Scantron 815-E forms, 4 blue books (or Scantron 886-E) OTHER RESOURCES: (website for the course text) (Mission College department website)

3 COURSE GRADE: Your Course Grade will be weighted as follows: 60% Lecture 40% Laboratory. 8 (of 10) Quizzes 12% of Grade (120 points) 2 (of 3) Exams 25% of Grade (250 points) Final Exam 18% of Grade (180 points) Article Review 5% of Grade (50 points) 3 Lab Quizzes 7.5% of Grade (75 points) 2 Lab Exams 15% of Grade (150 points) Unknown Identifications 10% of Grade (100 points) Lab Participation/Notebook 7.5% of Grade (75 points) 1000 points total Lecture: The lowest 2 quiz scores and lowest exam score will be dropped (final exam can NOT be dropped). The Final Exam will be comprehensive with approximately half of the questions pertaining to previous material. Exams and quizzes will consist of multiple choice, short answer and essay questions. Multiple choice questions are to be answered on Scantron forms. Short answer and essay questions are to be answered in Blue Books. Any make-up exams or quizzes will be short answer, essay ONLY. Lab: Students are expected to have read each lab exercise BEFORE class. Completed lab notebooks are to be turned in to the instructor periodically as indicated in the schedule. Lab participation scores will reflect the number of labs completed (e.g., 80% completed = 80% credit). Lab notebooks will be graded near the end of the course out of a possible 25 points. Unknown Bacteria identifications will be graded based on 1) doing the necessary tests, 2) presentation of procedures and results in the lab notebook, and 3) interpretation of the results to identify the unknown (see handout for more details). Grading Scale: 900+ pts (90-100%) A pts (78-89%) B pts (65-77%) C pts (55-64%) D pts (below 55%) F ATTENDANCE POLICY: Attendance is required and roll will be taken. You are responsible for any information, date changes, etc., presented in class, whether or not you are present. Students missing more than 2 consecutive classes may be dropped. Students given add slips must complete the process by Friday February 20 th. Students withdrawing from the class must do so by: Tuesday February 24 th to receive a refund Friday March 6 th to avoid receiving a W on your transcript Friday May 8 th to receive a W and avoid a non-passing grade

4 SPECIAL ACCOMMODATIONS: If you require special accommodations, religious or ADA, inform me IN WRITING within the first two weeks of the semester. You will need to provide the appropriate documentation after which we will arrange to meet your needs. CHEATING/ACADEMIC DISHONESTY: Each student is expected to do his/her own work on all assignments, reports, examinations, etc. Cheating in any form will at a minimum result in ZERO points for the assignment or test, and may result in an "F" for the course. Here is a list of some actions that are considered cheating: Talking during an exam Copying answers from someone else's paper Using notes of any kind during an exam Showing a fellow student your exam or passing information Turning in someone else's work (plagiarism) Providing your work for someone else to copy Taking a call or text-message on your cell phone (please turn them off!) If you have a question during an exam, quietly walk up to the instructor and whisper your question. Translation dictionaries are not permitted during exams. Exiting the room during an exam is not permitted. RECOMMENDATIONS FOR SUCCESS: This is a demanding class covering a lot of information. Here are some suggestions: do NOT fall behind in the course, keep up with the material on a weekly basis each time you study, spend a few minutes reviewing previous lessons read the relevant chapters in your textbook, hi-lite pertinent lines and add these notes to your class notes (always write as you read) use associations, acronyms to help you remember things create flash cards and form study groups if you find that helpful keep a neat, well-organized, up-to-date lab manual know the key terms (you can t answer questions correctly if you don t!) at a minimum, you should learn the course material 3 times in order to retain it well for the exams and quizzes: 1) comprehend the class material during the lecture 2) read the corresponding material in the text while reviewing your notes 3) review your notes and key terms before the exams

5 LECTURE SCHEDULE Week Date LECTURE TOPIC (textbook reading)* 1 Feb 10 Introduction, History of Microbiology (ch. 1) Review of Chemical Principles (ch. 2) Feb 12 Microbial Classification and Identification (ch. 10) Microscopy (ch. 3) 2 Feb 17 QUIZ: Eukaryotic, Prokaryotic Cell Structure & Function (ch. 4) Feb 19 Survey of the Domains Bacteria & Archaea (ch. 11) 3 Feb 24 QUIZ: Microbial Metabolism (ch. 5) Feb 26 Microbial Growth (ch. 6) 4 Mar 3 QUIZ: Bacterial Gene Expression & Regulation (ch. 8, pp ) Mar 5 Mutation & Bacterial Gene Transfer (ch. 8, pp ) 5 Mar 10 EXAM #1 (chapters 3-6, 8, 10 & 11) Mar 12 The Fungi (ch. 12A, pp ) 6 Mar 17 Parasitic Protozoa & Helminths (ch. 12B, pp ) Mar 19 QUIZ: Bacteriophages (ch. 13A, pp ) 7 Mar 24 Animal Viruses & Prions (ch. 13B, pp ) Mar 26 QUIZ: Control of Microbial Growth (ch. 7) 8 Mar 31 CESAR CHAVEZ HOLIDAY Apr 2 Antimicrobial Drugs (ch. 20) Apr 6-12 SPRING BREAK 9 Apr 14 QUIZ: Principals of Epidemiology (ch. 14) Apr 16 EXAM #2 (chapters 7, 12, 13, 14 & 20) 10 Apr 21 Non-specific (Innate) Defenses of the Host (ch. 16) Apr 23 Adaptive Immunity: Overview & Antigen Presentation (ch. 17A) 11 Apr 28 QUIZ: T Cells & Cell-Mediated Immune Responses (ch. 17B) Apr 30 Humoral Immune Responses/Antibody Structure, Function (ch. 17C) 12 May 5 QUIZ: Vaccination & Diagnostic Immunology (ch. 18) May 7 Disorders of the Immune System (ch. 19) 13 May 12 EXAM #3 (chapters 16-19) May 14 Microbial Mechanisms of Pathogenicity (ch. 15) 14 May 19 Selected Bacterial Pathogens (ch ) May 21 QUIZ: Selected Viral Pathogens (ch ) 15 May 26 Selected Eukaryotic Pathogens (ch ) May 28 QUIZ: Environmental Microbiology (ch. 27, pp ) Microbiology in Foods & Industry (ch. 28) 16 June 2 Comprehensive FINAL EXAM (8:00-10:00 in INST 2001) * The lecture notes will be available in PDF format through the department website or by . NOTE: Quizzes will cover material from the 2 previous lectures.

6 LABORATORY SCHEDULE Week Date LAB TOPIC 1 Feb 10 Lab Orientation Feb 12 Ex. 1: Use and Care of the Microscope 2 Feb 17 Ex. 3: Preparation of Smears and Simple Staining Feb 19 *Ex. 5: Gram Staining 3 Feb 24 Ex. 6: Acid Fast Staining Feb 26 Ex. 7: Endospore & Capsule Staining 4 Mar 3 QUIZ #1 / Ex. 8: Morphological Unknown Mar 5 Ex. 8 (follow-up) / Ex. 9: Microbes in the Environment 5 Mar 10 Ex. 9 (follow-up) / Ex. 10: Aseptic Technique Mar 12 Ex. 10 (follow-up) / Ex. 11: Dilution & Streak Technique 6 Mar 17 Ex. 11 (follow-up) / Ex. 34: Fungi Yeasts Mar 19 Ex. 34 (follow-up) / Ex. 35: Fungi Molds 7 Mar 24 Ex. 37: Protozoa Mar 26 **Ex. 35 (follow-up) / Ex. 22: Growth Control Heat 8 Mar 31 CESAR CHAVEZ HOLIDAY Apr 2 Ex. 22 (follow-up) / Ex. 23: Growth Control UV Light Apr 6-12 SPRING BREAK 9 Apr 14 Ex. 23 (follow-up) / Ex. 24: Growth Control Chemicals Apr 16 QUIZ #2 / Ex. 24 (follow-up) / Ex. 33: Biochemical Unknown given 10 Apr 21 Ex. 33 (follow-up) / Ex. 13: Carbohydrate Catabolism Apr 23 Ex. 13 (follow-up) / Ex. 14: Carbohydrate Fermentation 11 Apr 28 Ex. 14 (follow-up) / Ex. 15: Protein Catabolism I Apr 30 Ex. 15 (follow-up) / Ex. 16: Protein Catabolism II 12 May 5 Ex. 16 (follow-up) / Ex. 17: Respiration May 7 Ex. 17 (follow-up) / Ex. 18: Rapid Identification Enterotube II Ex. 52: Microbes in Water (bring water sample from home) 13 May 12 QUIZ #3 / Ex. 18 (follow-up) / Ex. 52 (follow-up) May 14 **LABORATORY MIDTERM EXAM 14 May 19 Ex. 52 (follow-up) / Ex. 25: Antimicrobial Drugs May 21 Ex. 25 (follow-up) / Ex. 54: Microbes in Food: Contamination 15 May 26 Ex. 54: (follow-up) May 28 LABORATORY FINAL EXAM *Turn in lab notebook at the end of lab. **Unknown due, turn in lab notebook at the end of lab. QUIZ #1 will cover Exercises 1, 3, 5-7. QUIZ #2 will cover Exercises 9-11, 22-24, 34, 35, 37. QUIZ #3 will cover Exercises

7 SCORE SHEET LECTURE POINTS LAB POINTS Quiz #1 Exam I Midterm Lab 1 Lab 22 Quiz #2 Exam II Lab Final Lab 3 Lab 23 Quiz #3 Exam III Unkn. #1 Lab 5 Lab 24 Quiz #4 Final Unkn. #2 Lab 6 Lab 33 Quiz #5 Article Quiz #1 Lab 7 Lab 13 Quiz #6 Quiz #2 Lab 8 Lab 14 Quiz #7 Quiz #3 Lab 9 Lab 15 Quiz #8 notebook Lab 10 Lab 16 Quiz #9 Lab 11 Lab 17 Quiz #10 Lab 34 Lab 18 Lab 35 Lab 52 Lab 37 Lab 25 Lab 54 LECTURE: Each lecture quiz will have 15 possible points (2 lowest scores will be dropped). Each of the first 3 exams will have 125 possible points (lowest score will be dropped). The final exam will have 180 possible points. The article review will have 50 possible points (details provided in handout). LAB: Each lab exam will have 75 possible points. If you are satisfied with your lab midterm score, you have the option of doubling that score and skipping the lab final exam. The morphological unknown identification (Unkn. #1) will have 30 possible points, and the biochemical unknown identification (Unkn. #2) will have 70 possible points (details provided in handout). Each lab attended will be worth ~2 pts. Your lab notebook will be graded out of 25 pts. To keep track of your performance throughout the course, add up your total points earned and divide by the total possible points thus far in the class. Multiply by 100 to get a percent score to compare with the grade scale on page 3 of the syllabus.