OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :20:05 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis Address of laboratory: Federal Institute for Risk Assessment Diedersdorfer Weg 1 D Berlin GERMANY Tel.: Fax: address: istvan.szabo@bfr.bund.de Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Istvan Szabo Name (including Title and Position) of OIE Reference Expert: Dr. Istvan Szabo Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally Direct diagnostic tests Nationally Internationally PCR no 300 Real-time PCR yes 36 MLVA no 26 Next Generation Sequencing no 500 Serotyping yes 3899 Test Salmonella Vaccines no 65 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 4. Did your laboratory produce vaccines? 2 OIE Reference Laboratory Reports Activities, 2016

3 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? OIE Reference Laboratory Reports Activities,

4 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Ecology from Farm to Fork Of microbial drug Resistance and Transmission 5 years Provide scientific evidence and high quality data on anti-microbial resistance (AMR) in the food chain various BELGIUM BULGARIA DENMARK FRANCE GERMANY ITALY POLAND SPAIN SWEDEN THE NETHERLANDS New approaches in identifying and characterizing microbiological and chemical hazards 2 years Capacity building of Next Generation Sequencing in for surveillance of foodborne pathogens (E.coli, Salmonella) various DENMARK GERMANY ITALY POLAND UNITED KINGDOM ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 5 Falgenhauer, L., Imirzalioglu, C., Ghosh, H., Gwozdzinski, K., Schmiedel, J., Gentil, K., Bauerfeind, R., Kämpfer, P., Seifert, H., Michael Brenner, G., Schwarz, S., Pfeifer, Y., Werner, G., Pietsch, M., Roesler, U., Guerra, B., Fischer, J., Sharp, H., Käsbohrer, A., Goesmann, A., Hille, K., Kreienbrock, L. and Chakraborty, T. (2016) Circulation of clonal populations of fluoroquinolone-resistant CTX-M-15-producing Escherichia coli ST410 in humans and animals in Germany. International journal of antimicrobial agents 47, Falgenhauer, L., Waezsada, S.-E., Gwozdzinski, K., Imirzalioglu, C., Irrgang, A., Fischer, J., Guerra, B., Käsbohrer, A., Goesmann, A. and Chakraborty, T. (2016) Chromosomal location of both mcr-1 and blactx-m-15 in fluoroquinolone-resistant Escherichia coli ST410. Emerging Infectious Diseases 22(9): Fischer, J., San Jose, M., Roschanski, N., Schmoger, S., Baumann, B., Irrgang, A., Friese, A., Roesler, U., Helmuth, R. and Guerra, B. (2016) Spread and persistence of VIM-1 Carbapenemase-producing Enterobacteriaceae in three German swine farms in 2011 and Veterinary Microbiology (16)30116-X. doi: /j.vetmic in press. Garcia, P., Malorny, B., Rodicio, M.R., Stephan, R., Hächler, H., Guerra, B. and Lucarelli, C. (2016) Horizontal acquisition of a multidrug-resistance module (R-type ASSuT) is responsible for the monophasic phenotype in a widespread clone of salmonella serovar 4,[5],12:i. Front Microbiol 7, OIE Reference Laboratory Reports Activities, 2016

5 Irrgang, A., Fischer, J., Grobbel, M., Schmoger, S., Skladnikiewicz-Ziemer, T., Thomas, K., Hensel, A., Tenhagen, B.- A. and Käsbohrer, A. (2016) Recurrent detetction of VIM-1 producing E. coli clone in German pig production Journal of Antimicrobial Chemotherapie. doi: /jac/dkw479 b) International conferences: 4 Borowiak, M., Rodriguez, I., del Castillo, B., Fischer, J., S., J., Baumann, B., Schroeter, A., Helmuth, R., B., G. and Malorny, B. (2016) Multidrug-resistant d-tartrate fermenting variant Salmonella enterica serovar Paratyphi B accumulated extended-spectrum beta-lactamase or AmpC beta-lactamase genes in Germany early in the 2000s. 5th ASM Conference on Salmonella. Potsdam, Germany: ASM (American Society for Microbiology). Fischer, J., Mateo S. J., Guiral, E., Irrgang, A., Schmoger, S., Guerra, B. and Kaesbohrer, A. (2016) Occurrence of ESBL-, AmpC- and carbapenemase- encoding genes in E. coli isolates from animal derived food-products in Germany. an Congress of Clinical Microbiology and Infectious Diseases. Amsterdam. Hadziabdic, S. (2016) In vitro Conjugal Transfer Frequency of Multidrug Resistant NDM-1 Carbapenemase Harboring Plasmid to Different Salmonella serovars Mainly Implicated in Human Salmonellosis. 5th ASM Conference on Salmonella. Potsdam, Germany: ASM (American Society for Microbiology). Irrgang, A., Fischer, J., Schmoger, S., Guerra, B. and Käsbohrer, A. (2016) Molecular characterisation of CTX-M-15 producing isolates from food in Germany. an Congress of Clinical Microbiology and Infectious Diseases. Amsterdam. c) National conferences: 4 Falgenhauer, L., Imirzalioglu, C., Ghosh, H., Gwozdzinski, K., Schmiedel, J., Gentil, K., Bauerfeind, R., Fischer, J., Seifert, H., Pfeifer, Y., Pietsch, M., Brenner Michael, G., Schwarz, S., Kaesbohrer, A., Roesler, U., Kämpfer, P., Kreienbrock, L., Goesmann, A. and Chakraborty, T. (2016) Circulation of blactx-m-15-encoding fluoroquinolone resistant clonal isolates in humans, animals, food and the environment in Germany. 68 Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie e V. Ulm, Deutschland. Roschanski, N., Pietsch, M., Pfeifer, Y., Fischer, J., Kreienbrock, L. and Roesler, U. (2016) Retrospective analysis of bacterial cultures sampled on chicken-fattening farms during the years revealed two additional VIM-1 positive isolates. 68 Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie e V Ulm, Germany. Schlez, K., Malorny, B., Szabo, I., Tietze, E., Rabsch, W., Eisenberg, T. and Zschöck, M. (2016) Gehäuftes Auftreten von Salmonella Subspezies I. der Serovarformel 6,7:-:1,5 bei Hessischen Wildschweinen (Severe occurrence of Salmonella subspecies I. 6.7: -: 1.5 in Hessian wild boars). Tagung der DVG-Fachgruppe "Bakteriologie und Mykologie" Volksbad, Jena. Szabo, I. (2016) Salmonellen in Nutztieren, Lebens- und Futtermitteln in Deutschland: Bericht aus dem NRL Salmonella (Salmonella in livestock, food and feed in Germany: An NRL-Salmonella report). In BfR-Symposium Zoonosen und Lebensmittelsicherheit. Bundesinstitut für Risikobewertung, Berlin. d) Other: (Provide website address or link to appropriate information) 1 Information system on consumer protection and food safety: FIS-VL Fachinformationssystem für Verbraucherschutz und Lebensmittelsicherheit ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? OIE Reference Laboratory Reports Activities,

6 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Certificate scan (PDF, JPG, PNG format) Akkreditierungsurkunde_2014.pdf 16. Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Serotyping of Salmonella spp. Detection of Salmonella spp. (ISO 6579) Accreditation body Detection of Salmonella spp. with PCR and real-time PCR Identification of Salmonella Enteritidis with real-time PCR Conformation of d-tartrat fermentation in Salmonella spp. with PCR MLVA of Salmonella isolates MLST of Salmonella isolates PFGE of Salmonella isolates 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) 6 OIE Reference Laboratory Reports Activities, 2016

7 ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 OIE Reference Laboratory Reports Activities,

8 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Participant EURL ring trial : Salmonella detection in food 34 EURL ring trial : Salmonella detection in bootsocks contaminated with chicken faeces 36 Participant EURL ring trial : Salmonella serotyping 36 Participant EURL ring trial : Salmonella PFGE typing 18 Organiser NRL ring trial: Salmonella detection in poultry pork faeces In phrame of the National Salmonella Control Program 43 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: 8 OIE Reference Laboratory Reports Activities, 2016