Lockheed Martin Biometrics. Finger and Palm identification Facial Recognition Fingerprint Analysis Iris Analysis DNA Typing

Transcription

1 Bret Light

2 Lockheed Martin Biometrics Finger and Palm identification Facial Recognition Fingerprint Analysis Iris Analysis DNA Typing

3 Lab Processing: 9 hrs, 50 min (average) 8 hrs, 15 min (rush) IntrepID S2A min

4 ZyGEM/Lockheed Martin IntrepID Compact, versatile footprint for IntrepID Totally-automated for skilled and unskilled users Flexible modular configuration for multiple enduser sectors (end-user serviceable) Cartridge design/size allows interfacing of IntrepID with robotic systems for HT Data compatible with all government databases No contact of reagents with the IntrepID (i.e., dry instrument) Compatible with all mobile devices and labbased systems 4

5 Lab-on-a-Chip in a Novel Cartridge cell phone cartridge custom swabs Compact versatile footprint (~size of 96-well plate) Extraction, PCR and separation are fully-integrated in a polymeric reagent-loaded cartridge Ultra thin design optimized for shipping and handling No contact between the instrument and sample or reagents in the cartridge All sample, reagents and waste are retained in cartridge Contamination is minimized Flexible DNA storage options Four independent and configurable sample channels Readily adaptable for other applications 5

6 Cartridge with Total Flexibility Three samples & AL Two sample & +/- controls One sample, +/- controls & AL Single sample Universal Design Two samples Three samples Four samples Unique design provides flexibility Analysis of any combination of samples, an allelic ladder and a variety of controls Configurable to meet the needs of the end-user No wasted lanes Reduced cost per sample Configurable for other sample types and applications Configurable Design Offers Efficiency, Performance, Flexibility 6

7 Integrated Sample Processing Processes Performed on Cartridge Swab in Tube (DNA) Instrument Interface Auto-Load Reagents Heat to Extract DNA Meter/Mix with PCR MasterMix PCR Fluidic Movement DNA Separation Detection Functionality resides in single, disposable cartridge with all reagents and fluidic architecture 7

8 Commercialization Microfluidic Circuitry: Timing Extraction, and Plans PCR, CE On-board Reagents, Self-Contained Fluidic Architecture, Full Integration

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10 Commercialization Loading Cartridge Timing into the and IntrepID Plans One step Cartridge Loading, Ready to Run

11 Simple Software Interface Standard User Scientist User

12 DNA Liberation Enzyme form organisms in exteraomophile environments such as Antarctic volcano Mt Erebus Enzymes are sometimes referred to as microbial metalloprotenases. This enzymes acts on proteins to break them down into small soluble peptides.

13 PCR done on Chip PCR done on Chip with IR Laser heating only the reaction not the plate Reagents can be loaded or sealed in plate Unique temperature monitoring technology and algorhythm PCR

14 Resolution and Separation Time PCR done on Chip Polymeric Microchip 8 minutes Capillary Electrophoresis 30 minutes Separation time reduced dramatically with novel polymer Detector provides comparable sensitivity to that of capillary electrophoresis

15 Temp ( C) Temp ( C) Separation Unique polymer allows separation in less that 5 Full profiles with strong signal-to-noise achieved No columns to change polymer is on board Optics self align for each run Channel time (sec) Channel time (sec)

16 Optics Automated Alignment Full independent control of x and y optics placement for all channels

17 IntrepID End-to-End Sample Processing Sample collection Sample Processing RESULTS 17

18 Rapid DNA Platform Partnerships Development of Rapid DNA Platform IntrepID Platform has been under development by ZyGEM and LMC for ~3 years Rapidly currently in pre-production and taking sales commercialization Rapid DNA Alliance Deployment of Rapid DNA Platform Work together to offer the IntrepID Platform nationally and internationally Customization to reflect regional and local needs Platform optimized for application Strength of Partnerships Delivers Maximum Customer Value 18