DNA fingerprinting of popular Indian varieties using SSR and ISSR markers

Transcription

1 DNA fingerprinting of popular Indian varieties using SSR and ISSR markers M.S.SARASWATHI et al. Senior Scientist (Hort.) National Research Centre for Banana Tiruchirappalli, Tamil Nadu, INDIA, Powerpoint Templates Page 1 By saraswathimse.com

2 INTRODUCTION Protection of biodiversity Identification of marker for specific traits Assessment of genetic diversity in crop germplasm Assist in the management and conservation of germplasm accessions Powerpoint Templates Page 2

3 MATERIALS S.No. Name of the variety Genomic group Sub group Special characteristics 1. Grand AAA Cavendish High yield Naine 2. Robusta AAA Cavendish 3. Dwarf Cavendish 4. Red banana AAA Cavendish High yield, ability to withstand strong winds, short crop cycle and good response to microirrigation and high economic returns per unit area AAA Unique Red Dacca 5. Poovan AAB Mysore Wilt resistant Pleasant flavoured banana with high carotenoids 6. Rasthali AAB Silk Mealy pulp with apple flavour 7. Nendran AAB Plantain Starchy fruits with high carotenoids, suitable for chips Powerpoint Templates making Page 3

4 Contd. 8. Pachanadan AAB Pome Coolant 9. Monthan ABB Monthan Vegetable 10. Kothia ABB Bluggoe Dual purpose variety 11. Karpuravalli ABB Pisang Awak 12. Udhayam ABB Pisang Awak Suitable for the preparation of value added products like fig, juice, wine etc. High yield and suitable for the preparation of value added products. 13. Neypoovan AB Neypoovan Soft and tasty pulp, suitable for the preparation of baby weaning foods. 14. Peyan ABB Peyan Pulp is juicy, sweet with aromatic flavours, orange coloured pulp. Resistant to Powerpoint Templates Yellow Sigatoka. Page 4

5 FOURTEEN POPULAR AND RULING COMMERCIAL VARIETIES Grand Naine Robusta Dwarf Cavendish Powerpoint Templates Page 5

6 Contd.. Red banana Poovan Rasthali Powerpoint Templates Page 6

7 Contd.. Nendran Pachanadan Monthan Powerpoint Templates Page 7

8 Contd. Kothia Karpuravalli Udhayam Powerpoint Templates Page 8

9 Contd. Neypoovan Peyan Powerpoint Templates Page 9

10 Methods ISSR (10) and SSR (20) markers RESULTS AND DISCUSSION ISSR Nine primers (90%) amplified products resulting in discrete, repeatable amplicons. Total alleles amplified Mean no. of alleles amplified per primer Maximum alleles amplified - 20 (UBC 811) Minimum alleles amplified - 7 (UBC 836) Powerpoint Templates Page 10

11 Unique bands produced by different ISSR markers Accession Name Primer pair Allele size in bp Red banana (AAA) UBC , 1258, 1366 UBC Poovan (AAB) UBC UBC Rasthali (AAB) UBC Nendran (AAB) UBC UBC Neypoovan (AB) UBC , 2117 UBC , 1637 Powerpoint Templates Page 11

12 M bp Grand Naine 2.Robusta 3.Dwarf Cavendish 4.Red banana 5.Poovan 6.Rasthali 7.Nendran 8.Pachanadan 9.Monthan 10.Kothia 11.Karpuravalli 12.Udhayam Powerpoint Templates 13.Neypoovan 14.Peyan Page 12

13 M bp 1366 bp 1258 bp 1.Grand Naine 2.Robusta 3.Dwarf Cavendish 4.Red banana 5.Poovan 6.Rasthali 7.Nendran 8.Pachanadan 9.Monthan 10.Kothia 11.Karpuravalli 12.Udhayam 13.Neypoovan 14.Peyan Powerpoint Templates Page 13

14 M bp 529 bp 608 bp 1.Grand Naine 2.Robusta 3.Dwarf Cavendish 4.Red banana 5.Poovan 6.Rasthali 7.Nendran 8.Pachanadan 9.Monthan 10.Kothia 11.Karpuravalli 12.Udhayam 13.Neypoovan 14.Peyan Powerpoint Templates Page 14

15 Common bands produced by varieties of the same genomic group in different ISSR markers Genome Varieties Primer Allele size in bp AAA 1.Grand Naine 2.Robusta UBC Dwarf Cavendish 4.Red banana AAB 1.Poovan 2.Rasthali 3.Nendran 4.Pachanadan UBC ABB 1.Monthan 2.Kothia 3.Karpuravalli 4.Udhayam 5.Peyan UBC Powerpoint Templates Page 15

16 Microsatellite markers Twenty primer pairs (100%) amplified products resulting indiscrete, repeatable amplicons. Total alleles amplified Mean no. of alleles amplified per primer 8.9 Maximum alleles amplified - 14 (AGMI 113/114) Minimum alleles amplified - 4 (Ma SSR 2-10 and Mb SSR 1-149) Unique alleles - 16 primer pairs (different varieties of the same or different genomic group) Common alleles (different varieties of the same genomic group) -14 primer pairs Powerpoint Templates Page 16

17 Unique bands produced by different microsatellite markers Accession Name Primer pair Allele size in bp Grand Naine (AAA) AGMI 67/68 127, 146 AGMI 95/96 272, 278 AGMI 103/ AGMI 113/ AGMI 123/ AGMI 197/ Ma 8a/8b 241 Ma 24a/24b 111 Robusta (AAA) AGMI 103/ AGMI 105/ AGMI 133/ Red banana (AAA) Ma SSR 24a/24b 115 Poovan (AAB) AGMI 24/ Ma SSR Rasthali (AAB) AGMI 35/ Powerpoint AGMI Templates 123/ Page 17

18 Accession Name Primer pair Allele size in bp Pachanadan (AAB) AGMI 67/ AGMI 105/ Nendran (AAB) AGMI 113/ Ma SSR Monthan (ABB) AGMI 103/ Kothia (ABB) AGMI 67/ AGMI 95/ AGMI 103/ AGMI 105/ AGMI 123/ AGMI 129/ Karpuravalli (ABB) AGMI 197/ Mb SSR Udhayam (ABB) AGMI 24/ Neypoovan (AB) AGMI 197, Peyan (ABB) AGMI 197/ Powerpoint Templates Page 18

19 Common bands produced by varieties of the same genomic group in different microsatellite markers Genome Varieties Primer pair Allele size in bp AGMI 33/34 129, 161 AAA 1.Grand Naine AGMI 35/ Robusta AGMI 93/94 125, Dwarf Cavendish AGMI 129/ Red banana Ma SSR 24a/24b 167 Mb SSR , 208 AAB ABB 1.Poovan 2.Rasthali 3.Nendran 4.Pachanadan AGMI 67/68 121, 126 AGMI 95/ AGMI 103/ , 199 AGMI 113/ AGMI 129/ , 217 AGMI 197/ Ma SSR 24a/24b 167, 172, 176 Ma SSR , 159 Ma SSR Mb SSR Monthan AGMI 129/ Kothia AGMI 103/ Karpuravalli Ma SSR UdhayamPowerpoint Templates 5.Peyan Page 19

20 DNA fingerprints produced by AGMI 113/114, Mb SSR and AGMI 95/ bp 196 bp M - Marker 1, 15, 29 Peyan 8, 22, 36 - Nendran 2, 16, 30 - Ney poovan 9, 23, 37 - Rasthali 3, 17, 31 Udhayam 10, 24, 38 - Poovan 4, 18, 32 Karpuravalli 11, 25, 39 - Red banana 5, 19, 33 Kothia 12, 26, 40 - Dwarf Cavendish 6, 20, 34 - Monthan 13, 27, 41 - Robusta 7, 21, 35 Pachanadan Powerpoint Templates 14, 28, 42 - Grand Naine Page 20

21 Primer Label Product range (bp) Number of alleles Expected Observed Expected observed AGMI 24 / AGMI 33/ AGMI 35/ AGMI 67/ AGMI 93/ AGMI 95/ AGMI 103/ AGMI 105/ AGMI 113/ AGMI 123/ Powerpoint Templates Page 21

22 AGMI 129/ AGMI 133/ AGMI 197/ Ma SSR 08a/08b Ma SSR 24a/24b Ma SSR Ma SSR Ma SSR Mb SSR Mb SSR Powerpoint Templates Page 22

23 Primer Label Polymorphic Information content Effective Multiplex ratio Marker Index AGMI 24 / AGMI 33/ AGMI 35/ AGMI 67/ AGMI 93/ AGMI 95/ AGMI 103/ AGMI 105/ AGMI 113/ AGMI 123/ AGMI 129/ AGMI 133/ AGMI 197/ Ma SSR 08a/08b Ma SSR 24a/24b Ma SSR Ma SSR Ma SSR Mb SSR Mb SSR Powerpoint Templates Page

24 Primer Label Polymorphic Information content Effective Multiplex ratio Marker Index UBC UBC UBC UBC UBC UBC UBC UBC UBC Powerpoint Templates Page 24

25 ISSR SSR Powerpoint Templates Page 25

26 Conclusion Genetic identity in terms of DNA fingerprints developed in the present study would have greater applications in aiding variety registration, DUS characterization and in detecting infringement of breeders rights and biopiracy. Specific bands could also be converted into STS markers of great value to detect any mix up between the cultivars and as DNA fingerprints. Powerpoint Templates Page 26

27 Powerpoint Templates Page 27