Mycotic Diseases. specimens from U.S. personnel and dependents were positive for C. albicans; two were posltive for

Transcription

1 Mycotic Diseases Coordinator : Howard E. Noyes, P~.D., Chief, Department of Bacteriology & Mycology Principal investigators : Renoo Kotrajaras, M.D.* Howard E. Noyes, Ph.D. Associate Investigators: Yupin Charoenvit, M.S. Mallnee Udhltanonda, B.S. OBJECTIVE: The obiectlve of these studtes is to gather information on the prevalence and distributlon of mycoses in this area of the world. In addition to these survey actlvltles, one study was carried out to determine the in vitro effects of griseofulvin on morphological changes of selected dermatophytes Isolated in Thalland and another was done to identify the bacterial components of dermatologic Infections. DESCRIPTION: Survey studies were prompted by the moior medical problems the dermatophytic fungi can present to the military In times of stress. Spectmens were usually collected by a member of this department from patients with dermatologic problems. Cultures were prepared by cleansing the leslon with 70 percent ethanol and transferring matertal (hair, sktn, nail) directly to 2 plates of Sabouraud-Cyclohexlmlde- Chloramphenicol medium. The plates were sealed with paper tape to prevent contamination, and periodtcally,examined during a 21 day Incubation at 25 C. Blood agar plates were also inoculated and incubated at 37 C when cllnlcal appearance of the lesion suggested bacterial infectlon. Thirteen recent isolates of dermatophytes were exposed to concentratlons of grlseofulvln ranglng from 0.1 to 30 mcg/ml In Sabouraud-Cycloheximide-Chloramphenlcol agar or broth. Colonial and microscopic changes were observed at 5, 10 and 15 days after inoculation and incubation at 25 C. In the study of the bacterial component of dermatologlc infections, culture sites for bacteria and fungl included the site of the lesion, canal of the right ear, right nostril, beneath the index fingernail of the right hand, perlneum and the fourth toeweb of the rlght foot. Procedures listed above were used for fungus cultures and a battery of culture media designed to favor different genera were used for bacterial cultures. PROGRESS: During the period covered by this report 755 roufine clinical specimens were received for mycological examinations. Included were 212 from Women's Hospital, and 36 from the U.S. Embassy Medical Unit and the 5th Field Horpital. Results In Table 1 show that the organisms most frequently isolated from Thai patients were Trlchophyton - rubrum and Candida - albicans. Five of 36 dermatologlc specimens from U.S. personnel and dependents were positive for C. albicans; two were posltive for -- T. rubrum and 1 was posltive for Trichophyton mentagrophytes able-2). In specimens from other than - human sources there were 5 isolates of Microsporum canis from 6 gibbons and 5 isolates of Mlcrosporum gypseum from 246 soil samples (Table 3). The study of the interaction($) of dermatophytes and grlseofulvin was undertaken because of concern to clinicians about the likelihood that griseofulvin-resistant strains of dermatophytes would emerge and the possibility that resistant forms would have different characteristics which would complicate their identiflcailon. This study was concerned wtth sequenttal macroscopic and mlcroscoplc changes of recently Isolated dermatophytes in the presence of increasing concentrations of griseofulvin. * Dermatologist at Women's Hospital, Bangkok, Thalland 6 1

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