Label-free Assays in a Drop. Protein Presence. Quantitation. Kinetics. Assay Development.

Transcription

1 Label-free Assays in a Drop Protein Presence. Quantitation. Kinetics. Assay Development.

2 Drop. Read. Done. Give BLItz a 4 µl drop of your sample and it does the rest. Its Dip and Read assays utilize disposable, ready-to-use biosensors that truly simplify label-free analysis. BLItz lets you analyze column fractions, monitor protein expression, do reagent QC, identify mechanism of action and run protein engineering studies right at your lab bench.

3 Little Genius. BLItz brilliantly packs the power of Dip and Read label-free analysis into a personal assay system. Small enough to fit on the palm of your hand (about the size of this page) and ready to go right out of the box, it can go anywhere you do. BLItz is also cleverly priced so everyone can have their own little genius.

4 Protein Presence: Instantly. Binding (nm) Neg. Control Unknown Pos. Control Time (sec) BLItz detects the presence or absence of active proteins in just a drop of sample, and lets you easily identify a specific protein in complex solutions. BLItz compliments existing lab methods such as blots and gels, and gives you real-time binding curves for visual confirmation in seconds so you can be confident in your results.

5 Protein Quantitation: Faster. Binding Rate (nm/s) Unknowns Fitted Curve Standards Concentration (µg/ml) BLItz measures concentration of your proteins without labeling or secondary reagents even in crude media. Accurate quantitation is obtained across a 5-log dynamic range with sensitivity down to just a few ng/ml. Results that can take several hours to generate with gels, HPLC and ELISA are ready in just a few seconds with BLItz.

6 Kinetics: Effortless. Binding (nm) nm 66.7 nm 33.3 nm 16.7 nm 8.3 nm Time (sec) Even though BLItz is small in both size and price, the label-free kinetic data it delivers is exceptional so there is no compromise. BLItz gives you rate and affinity constants for binding interactions (k a, k d, K D ) in minutes. Simple workflows using a drop of sample and a microfluidics-free design let you run kinetic assays at your personal lab bench with ease.

7 Assay Development: Smarter. Binding (nm) Biotin-Ms anti IL-5 MAb + hil-5 + Goat anti IL-5 PAb Biotin-Ms anti IL-5 MAb + hil-5 + Rat anti IL-5 MAb Time (sec) Immunoassay development can take days or even months. BLItz lets you see the effects of changing assay conditions on binding interactions in real-time, so you can develop assays in minutes. Quickly find the best antibody pair, identify optimal conditions for a specific parameter or build and optimize an assay step by step for use on other platforms.

8 About BLItz Technology Bio-layer Interferometry (BLI) Sample volume 4 µl Dimensions 6.8 H x 6.0 W x 8.7 D (17.4 cm H x 15.3 cm W x 22.2 cm D) Weight 7.2 lb (3.3 kg) Power V AC, A, 50/60 Hz, 8 W (18 W peak) Biosensors Anti-Human Fc Capture (AHC) Anti-mIgG-Fc Capture (AMC) Anti-Human IgG Quantitation (AHQ) Anti-Murine IgG Quantitation (AMQ) Aminopropylsilane (APS) Amine Reactive (AR2G) Anti-Human Fab-CH1 (FAB) Anti-GST (GST) Anti-Penta-HIS (HIS) Ni-NTA (NTA) Protein A (ProA) Protein G (ProG) Protein L (ProL) Streptavidin (SA) Super Streptavidin (SSA) To Order BLItz system with BLItz Pro software and starter kit: P/N Learn More If you analyze proteins, you should use BLItz! Visit BlitzMeNow.com or call 855.BLITZ.ME. Pall ForteBio Corp Willow Road, Suite 201 Menlo Park, CA t: 888.OCTET-75 or Pall ForteBio Europe 83 Victoria Street, Suite 407 London, SW1H 0HW, UK t: +44-(0) Pall ForteBio Analytics (Shanghai) Co., Ltd. 917 Halley Road, Bldg 4 Zhangjiang High Tech Park Shanghai, China t: ForteBio, Inc., ForteBio, BLItz, BLItz Pro, Dip and Read and the ForteBio logo are trademarks and/or registered trademarks of Pall ForteBio Corp. P/N , Rev B