Genomics Blood DNA extraction and DNA normalization DNA is extracted from full blood, normalized for long term storage and for PCR reactions

Transcription

1 Genomics Blood DNA extraction and DNA normalization DNA is extracted from full blood, normalized for long term storage and for PCR reactions Technical Reference EU Input Blood samples The blood samples are loaded on the workstation in 96-tube rack format Process DNA extraction and normalization Barcode scanning of rack and tubes Blood transfer to Promega Maxwell 16 for DNA extraction DNA storage in 96-tube racks DNA quantification by Trinean Dropsense microplate reader Volume determination by Brooks TubeAuditor TM Normalization and aliquoting in 96- tube racks Output Normalized DNA aliquots The normalized DNA aliquots are stored in 96-tube rack format or any other plate types. ( see rack type editor)

2 System Overview # Type of module Brand, model 1. Automation platform Tecan Freedom EVO Arm 1 LiHa8 Liquid Handling arm equipped with 8 fix tips 3. Arm 2 RoMa long Long Robotic Manipulator with excentric grippers 4. Arm 3 LiHa8 Liquid Handling arm equipped with 8 fix tips 5. DNA extractor 1 Promega Maxwell 16 AS2000-HS 6. 2D BC reader Ziath DataPaq High Speed Single Rack Scanner ZTS-A6 7. DNA extractor 2 Promega Maxwell 16 AS2000-HS 8. Tube volume measurement Brooks Tube Auditor 9. Droplet reader Trinean DropSense 96 microplate reader 10. Freezer Liconic STX44 deep freezer, benchtop version 11. Hood Customized laminar flow hood, with HEPA filtration unit on top 12. 2D BC reader Datalogic Matrix 200 2D Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 2/7

3 Highlights: TIG parts Magnetic bead leftover separation from eluted DNA A customized magnetic carrier (left picture, carrier in the center) was specifically designed in order to remove any traces of magnetic beads from the blue DNA collection reservoirs. At DNA extraction completion, the extractor slide holding the reservoirs with eluted DNA is moved against the magnetic carrier (central picture). The bead leftover is attracted by the strong neodymium magnets fixed on the carrier (right picture, beads are the black particles towards the magnets). The LiHa can then aspirate the eluted DNA from the reservoirs without collecting any beads and transfer it to the ABgene racks. Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 3/7

4 Highlights: Customized integration of Tecan modules Tecan Freedom EVO with customized arm configuration LiHa / RoMa Long / LiHa In order to perform 2 x 16 DNA extractions in parallel, two LiHa arms were installed on the Freedom EVO 200. The left LiHa arm was pipetting blood samples to the cartridges of the left extractor, while the right arm was executing the same process on the robot right end side. The Rack Type Editor is a new feature in the Extractor application software. It allows defining any type of destination racks for the Normalization DNA aliquots. According to the defined code in the Editor for each rack type, the system recognizes automatically which rack is on the worktable and loads the correct labware in Freedom EVOware for the pipetting step. Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 4/7

5 Highlights: 3 rd party device integration Integration of the Promega Maxwell 16 Promega Maxwell 16 unit extracting DNA from 8 blood samples (left picture). The magnetic beads on the white plungers are drying out in the air for 5 minutes. Buffer cartridges with released plungers at process end (right picture). The DNA is dissolved in the elution buffer in the blue reservoirs. The magnetic beads are attracted by powerful neodymium magnets in front of the reservoirs. Integration of 2D BC reader Datalogic Matrix 200 Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 5/7

6 Integration of the Trinean Dropsense reader The reader is integrated below the worktable and can be accessed by the RoMa Long Pipetting 2 l into the small input funnel of the DropSense plate for the reader Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 6/7

7 Integration of the Brooks TubeAuditor Integration of the TubeAuditor below worktable The tube rack was positioned on the dedicated tray by RoMa long Typical picture of tubes taken by the TubeAuditor for volume assessment Scientific instrumentation; not for use in human, clinical or diagnostic procedure. All systems or products are tailor-made one-offs that were exclusively developed on customer demand. All displayed pictures and images originate from development-, test- or installation phases and do not claim to represent the final installation at customer site. All mentioned trademarks are protected by law. In general, the trademarks and designs referenced herein are trademarks, or registered trademarks, of Tecan Group Ltd., Männedorf, Switzerland. A complete list may be found at Product names and company names that are not contained in the list but are noted herein may be the trademarks of their respective owners. 2014, Tecan Trading AG, Switzerland, all rights reserved. For disclaimer and trademarks please visit Manufactured by TIG, Tecan Schweiz AG, Seestrasse 103, 8708 Männedorf Switzerland, TIGflyer p 7/7