Overview of Agilent s MS Portfolio

Transcription

1 Overview of Agilent s MS Portfolio 1

2 5977E SQ 7820 GC 5977A SQ 7890 GC Agilent GC/MS More Choices Better Solutions 5975T LTM SQ 7000 TQ 7890 GC 7200 Q-TOF 7890 GC

3 Agilent GC/MS Portfolio Different Analyses Require Different Solutions Ultimate combination of qualitative and quantitative capability Highest speed & sensitivity; New MassHunter SW Q-TOF GC/MS (in R&D) Triple Quad GC/MS Demanding analyses (R&D and routine) (metabolomics, food, toxicology) High-throughput, high sensitive targeted quantitative analyses (food, drugs, environmental) Industry-standard workhorse Single Quad GC/MS Routine analyses (environmental, food, drugs, pharmaceutical, chemical, materials) Foundation: Industry-leading performance, usability, reliability, and support

4 Agilent GC/MS Portfolio in FY 14 Q-TOF 7890 GC Performance 5977A SQ 7890 GC 5975T SQ 7000 TQ 7890 GC 5977E SQ 7820 GC with Tray support $50k Price Page 4

5 New 5977 Series GC/MSD Improving the Industry Standard for GC/SQ! 5

6 Expanded Capabilities: 7890B/5977A Improving the Industry Standards for GC/SQ Higher Ion Count Extractor EI source Increased ion count S/N > 1500:1 IDL < 10 fg (industry first) Improved thermal profile Save Time & Energy Sequence Scheduler Standby post run Ready to run from standby Bake Mode as needed Factory backflush option Fast Vent capability Better Vacuum by Agilent Pump New Agilent V304 Turbo TwisTorr Molecular Drag technology Improved pumping light gases H 2 (220 L/s); He (255 L/s) Agilent Floating Suspension Longer life Quiet operation Safety/Protection Vacuum failure Shut off gas (He or H2) Faster Scanning 20,000 u/s But best choice of fast scanning GC/MS is GC/QTOF 7890B GC Features EMF, calculator, etc. All of 5975 s features also built in 5977, such as Hot Gold Quad, SIM/Scan, Gain tuning, Triple-Axis Detector, Trace Ion Detection, Auto CI, etc..

7 Agilent 5977E with 7820 GC A Cost Effective Solution for GC/MS 5977E Bundled system that contains a GC, MS, SW loaded on a PC and a printer. Software includes both ChemStation and MassHunter data analysis ALS and Tray 5977 MSD Two EI versions: Diffusion pump 304FS turbo stainless steel source 7820 GC Includes low bleed septa, S/S inlet & liner, and conditioned HP- 5ms column Optional inert EI source available

8 New Agilent 7000C Triple Quadrupole GC/MS 11

9 GC/MS for Targets, Non-targets and Unknowns Targets, non-targets & unknowns Targets with higher sensitivity Targets with higher selectivity & sensitivity SQ GC-MS scan GC-MS SIM (SIS) GC/MS/MS TQ TOF has no SIM or SIS equivalent TOF GC/MS scan Higher resolution Accurate mass Higher data rate Better sensitivity Targets, non-targets & unknowns Q-TOF GC/MS/MS Product ion spectra Higher resolution Less sensitivity Targets with higher selectivity & structural elucidation

10 7890B GC and 7000 Series Triple Quad GC/MS Blend of Proven, High Performance Features GC capabilities are even more valuable for MS/MS trace analysis of complex matrices: And all the benefits of the 7890B GC 7893 Automatic Liquid Sampler (ALS) Multi-mode Inlet (MMI) Backflush configurations using the Pressure-Controlled Tee (PCT) Integrated intelligence features including Parts Finder, Backflush Wizard and Sleep/Wake mode

11 7890B-7000C GC/MS/MS: Blend of Proven, High Performance Features High sensitivity Extraction Source highest operating temperature (up to 350ºC) and most inert materials The world s only MS/MS with two hot (up to 200ºC) quartz, hyperbolic quadrupole analyzer The world s only MS/MS high pressure, linear collision cell with helium quench technology for ultra low neutral noise The world s only MS/MS with new ramped-iris, doubly off-axis HED-EM and triple channel EM High productivity MassHunter software for GC/MS/MS

12 7000C MS/MS: Built on Proven Technologies The best technologies from the industry leading GC/MSD Improved Extractor EI source Heated monolithic gold plated quartz quadrupole Reliable, inert, high performance ion source High Signal/Low Noise Triple- Axis Detector PCI/NCI ion source and the LC/TQ Linear acceleration enhanced hexapole collision cell MassHunter software Inert, high temp, extraction EI source Split Flow Turbo Gold plated quartz, hyperbolic quadrupoles Ramped Iris Triple- Axis Detector High pressure hexapole collision cell with Patent Pending Helium Quench Gas Hinged access to source and detector

13 7000C Increased Signal and Improved Thermal Characteristics Extractor source cutaway Extractor Source w/ Ramped Iris Detector Push-Pull Principle: Repeller and Extractor Standard Source & Detector mode

14 7000 Series High Performance Collision Cell High pressure, linear acceleration design (Cell housing and 2 rods removed for clarity) Fast SRM (MRM) up to 800 transitions/sec allows more compounds per ion group No cross-talk even at 0.5 msec Wide mass bandwidth for optimum sensitivity without compound specific tuning Quadrupole post-filter Support cradle Quadrupole pre-filter Patent pending Helium Quenching reduction of neutral noise (increased S/N)

15 2 x Benefit from Helium Quench + MRM ( > ) 1pgHCB_std_cond_UnkGain_2090EMV_He0pct_05.d Noise (RMS) = 20.08; SNR (6.470min) = Helium Quench Gas OFF Same Signal tested with hexachlorobenzene x Noise Decreased + MRM ( > ) 1pgHCB_std_cond_UnkGain_2090EMV_He0pct_05.d Noise (RMS) = 20.08; SNR (6.470min) = S/N 341: x Counts vs. Acquisition Time (min) MRM ( > ) 1pgHCB_std_cond_UnkGain_2090EMV_He20pct_04.d Noise (RMS) = 6.03; SNR (6.465min) = Helium Quench Gas ON S/N 1294:1 Same Signal Counts vs. Acquisition Time (min) Counts vs. Acquisition Time (min) In MS apps, neutral noise is not visible it is buried by more intense chemical noise. Since MS/MS eliminates chemical noise, neutral noise is visible and must be minimized.

16 Why Helium Quenching? Collision Cell Process: Full Description for GC/MS/MS Source He* + Quad Analyzer Collision Cell Precursor Ions In 1 ml/min N 2 Collision Gas collision induced dissociation Quad Analyzer Product Ions Out At the detector, metastable helium generates neutral noise. + He* Detector A high population of highly energetic helium metastables are produced in an EI source; since metastable helium is not charged, it can pass through mass analyzer field into the collision cell and through to the HED- EM In GC/MS, neutral noise is buried in much higher chemical noise. In GC/MS/MS, chemical noise is greatly reduced so neutral noise is a critical source of noise.

17 Agilent Collision Cell Process with Quench Gas Collision Cell 1 ml/min N 2 Collision Gas Quad Analyzer Quad Analyzer Source He* + Precursor Ions In collision induced dissociation Product Ions Out + He* Detector He Buffer Gas He* + He 2 He + heat Transmission of metastable helium to the detector is greatly reduced; the Triple-Axis detector further reduces neutral noise for ultra-low neutral noise.

18 Ramped Detector Iris Improved high mass peak shape Improved mid to high mass sensitivity Mass Iris (V) Signal Increase X X MS2 502 Peak Shape X X X X

19 Note: IDL can only be demonstrated if an autosampler is part of the installed system. Note: Reference specs represent typical performance and are not confirmed at installation. 22

20 7000 Series Triple Quad GC/MS Applications Pesticide Multiresidue in food and environmental samples Fastest MRM speed for increased quantitation accuracy of trace contaminants Rugged ion source and quadrupole design for maximized uptime Other: doping control, PAH, PCB, dioxins, veterinary drugs

21 24 Agilent 7200 GC/QTOF

22 Why the Demand for High Resolution and Accurate Mass MS and MS/MS? The Analytes Are the Same (MS or MS/MS) Demand Is Driven by the MATRIX COMPLEXITY High Res & MS/MS Increase Selectivity Demand Is Driven by the ANALYSIS OF NON_TARGETS & UNKNOWNS Accurate Mass, Especially with MS/MS, Greatly Facilitates Qualitative Analysis

23 Proven Technology and Performance 4GHz ADC electronics enable a high sampling rate (32 Gbit/s) which improves the resolution, mass accuracy, and sensitivity for low-abundance samples. Dual gain amplifiers simultaneously process detector signals through both lowgain and high gain channels, extending the dynamic range to 105. Hot, quartz monolithic quadrupole analyzer and collision cell identical to the 7000 Quadrupole MS/MS New Removable Ion Source includes repeller, ion volume, extraction lens and dual filaments Dual-stage ion mirror improves second-order time focusing for high mass resolution. Proprietary INVAR flight tube sealed in a vacuum-insulated shell eliminates thermal mass drift due to temperature changes to maintain excellent mass accuracy, 24/7. Added length improves mass resolution. Analog-to-digital (ADC) Detector: Unlike time-to-digital (TDC) detectors which record single ion events, ADC detection records multiple ion events, allowing very accurate mass assignments over a wide mass range and dynamic range of concentrations. New Internal Reference Mass can be delivered to the source at a low and high concentration Two 300L/s turbos pump the focusing optics and flight tube Split-flow turbo differentially pumps the ion source and quadrupole analyzer compartments Hexapole collision cell accelerates ion through the cell to enable faster generation of high-quality MS/MS spectra without cross-talk

24 Removable Ion Source Automated Gate Valve RIS Automated Retractable Transfer Line

25 7200 Q-TOF Specifications Resolving Power: > 12.5K (> 13K typical) Mass Accuracy: < 3 ppm (< 2 ppm typical) EI Instrument Detection Limit: < 250 fg < 8% RSD for 8 consecutive injections EI full spectrum S/N: > 2000:1 for 1 pg OFN Dynamic Range: > 3 orders of magnitude TOF range: Da MS/MS range: Da Spectra/Sec: up to 50 Hz

26 Resolving Power R = 614/0.68 = 903 Mz=614 Single Quad or Ion trap Resolving Power, R=Mass/PW Pw=0.68 TOF

27 GC/QTOF Markets Flavor and Fragrances Forensics and Toxicology Metabolomics Natural Products Food Safety and Testing Environmental Energy and Petrochemical

28 Agilent LC/MS Portfolio 31

29 32 Agilent 6100 Series LC/MS SQ

30 Agilent 6100 Series LC/MS SQ Portfolio Value and Performance 6150 High-End 6130 Mid-Range Fastest scan speed and most sensitive SQ on the market Best at confirmation of compound with accurate isotope ratios More sensitive than competition Extended mass range for Biopharma Performance 6120 Entry-Mid Great value for the academic or start-up lab Great reliability for the pharmaceutical lab

31 Key Applications Confirmation of Synthesis, Impurity Analysis, Product Analysis Confirmation of Synthesis Impurity Analysis Confirmation of Product Uniformity Verify Low Levels of Impurities Confirmation of Synthesis Core Labs Training Labs Pharma Chemical Academia Mass spectrometry data has become essential to the chemist: Did I make the right molecule? What are the impurities/side reactions? What is the purity of the product?

32 35 Agilent 6230 Accurate Mass TOF

33 Accurate Mass TOF Design Generates accurate mass data at the sub-ppm level

34 Agilent Jet Stream Ion Generation-6230 Gas Dynamics View Enhanced efficiency nebulizer Nebulizing gas Super-heated sheath gas Nozzle voltage Heated drying gas The super-heated sheath gas collimates the nebulizer spray and creates a dramatically brighter source Patent Pending Resistive sampling capillary

35 What is the Benefit of Accurate Mass? Confidence in Compound Identification! Reserpine (C 33 H 40 N 2 O 9 ) has MH + at Da Single quad gives mass to +/- 0.1 Da = 165 ppm Number of possible elemental formulae using only C, H, O & N: 165 ppm ppm 13 5 ppm 7 3 ppm 4 2 ppm 2 Accurate mass reduces number of candidates and risk of investing effort with the wrong molecule

36 Benefit of Large Dynamic Range Detecting trace levels of pesticides in complex matrix Atrazine herbicide found in tomato extract at a level 500 times lower than the base peak x TOF Spectrum x Atrazine -1 ppm mass error Mass-to-Charge (m/z) Mass-to-Charge (m/z)

37 Agilent 6230 Accurate Mass TOF LC/MS System Value and Performance Performance 6230 With AJS 6230 Option 200 Best mass accuracy Protein Characterization Capillary electrophoresis - MS Best mass accuracy Value for academic or start-up lab Confirmation of protein synthesis

38 Key Markets and Applications Pharma, Biopharma, Chemical, Forensics Confirmation of Synthesis Impurity Analysis Pharma Confirmation of Product Uniformity Screening for undesirable compounds Chemical Drug screening Drugs of abuse Opiates Designer drugs Oral fluids Forensic Toxicology Confirmation of Protein synthesis Walkup: multi-user setting Protein ID Benefits of Accurate Mass TOF in these markets 1. Mass Accuracy ensures confident compound identification 2. Comprehensive screening allows for re-examination of data at a later time for additional compounds 3. Simplified optimization compared to Triple Quad

39 Agilent 6400 Triple Quadrupole LC/MS 42

40 6400 QQQ LC/MS Portfolio Positioning Q1 FY14 Excellent Value and Performance 6495A High-End 6460C Core Mid-Range 6460C Option 100 Mid-Range 6420A Entry-Level Ultra-Trace Analysis Agilent ifunnel Technology Attogram zeptomole sensitivity! Ideal for biomarker, steroids, and most demanding bioanalytical/clinical assays Trace Analysis Agilent Jet Stream Technology Sub femtogram sensitivity! Ideal for multi-residue pesticide analysis, vitamin D, and bioanalysis Routine Trace Analysis 6460 with ESI Source Femtogram attomol sensitivity and Jet Stream Ready Ideal for drugs of abuse, chemical and forensics Routine Analysis Lowest cost of ownership, Robust and Reliable Excellent sensitivity and productivity Ideal for clinical research & drug analysis Performance QQQ LC/MS Overview 10/29/2014

41 Key Markets & Applications for 6400 QQQ LC/MS Biomarkers High sensitivity dmrm tmrm Protein Quant Immunosuppressants Pain management Vitamin D Endogenous steroids Amino acids Clinical Pesticides Mycotoxins Antibiotics Allergens Food profiling Food Water Pharmaceutical compounds Contaminants Online SPE Environmental Research Chemical Drug discovery in vitro ADME PK analysis Bioanalysis Kits & Methods tmrm Drug screening Drugs of abuse Opiates Designer drugs Oral fluids Academia Pharma Veterinary Drugs Forensic Toxicology

42 Agilent 6400 QQQ LC/MS Price & Performance Relative Performance Routine Analysis PPCPs, PFCs Opiates Pain management Chemical & Forensics Trace Analysis Pesticide, Mycotoxins Vet drugs, Designer drugs Drug of abuse Vitamin D Bioanalysis 6420 QQQ Ultra-Trace Analysis Pesticide, Hormones Emerging contaminants Biomarker, Steroids Ultra-low Assay Requirements Complex matrices Relative Price 6460 QQQ AJS 6460 QQQ Option 100 ESI 6430 QQQ Obsolete Nov 1 st QQQ Note 1: Circles size indicates QQQ sales Note 2: Data are based on 2013 performance specs and ASPs

43 Proven ifunnel Technology Agilent Jet Stream Hexabore Capillary Dual Ion Funnel Thermal gradient focusing Efficient desolvation Creates an ion rich zone Six capillary inlets Samples x10 times more ion rich gas Removes the gas but captures the ions Removes neutral noise 10/29/ Agilent 6495 QQQ LC/MS System

44 Agilent 6500 QTOF LC/MS Portfolio 47

45 6500 QTOF LC/MS Portfolio Excellent Value and Performance 6550 High-End Agilent ifunnel Technology Low femtogram sensitivity in complex samples Qual/quan in food, pharma Greater information for proteomics, metabolomics and glycomics 6540 Mid-Range Agilent Jet Stream Technology mid femtogram sensitivity Enhanced resolution and mass accuracy for complex samples Ideal for impurity analysis, metabolite ID, pesticide tests Performance 6530 Entry Level Ease of use and robust operation Excellent value for everyday routine use Ideal for chemical, environmental and forensic applications

46 Key Markets & Applications for 6500 QTOF LC/MS Biomarkers Targeted proteomics Pathway Studies Lipids Sugars Pesticides Mycotoxins Antibiotics Allergens Food profiling Water Pharmaceutical compounds Contaminants Protein ID Metabolo mics Food Environmental Discovery Research OMICS studies Drug discovery Qual/ Quan Impurities Metabolite ID Drug characterization PTM screening Glycan analysis Drug screening Drugs of abuse Designer drugs Oral fluids Academia Pharma Biopharmas Forensic Toxicology

47 6560 Ion Mobility QTOF LC/MS System Reveal Greater Detail 6560 Ion Mobility Performance without compromise for ion mobility and Q-TOF modes Added dimension of separation power Preserves structural characteristics of molecular conformation Direct measurements of collision cross sections

48 6560 Ion Mobility QTOF Key Applications Lipids Analysis Compound Class Separations Targeted Proteomics Biopharmaceutical Characterization Natural Products Characterization Initial applications - CCS studies and IM separations

49 Agilent 6500 QTOF and IM QTOF LC/MS Price & Performance 100 Ultra-Complex Analysis Lipidomics Proteomics Glycomics Biophysical studies Polymers Biopharm drug studies 6560 IM QTOF Relative Performance Routine Analysis PPCPs, PFCs Impurity analysis Chemical & Forensics Quality control Trace Analysis 6550 ifunnel QTOF Pesticides, Mycotoxins Protein ID/ Biomarkers Metabolomics Qual/ Quan 6530 QTOF 6540 QTOF 0 Relative Price

50 THANK YOU