CASY Model TTC E L E C T R I C A L C U R R E N T EXCLUSION ECE BASED CELL ANALYSIS

Transcription

1 CASY Model TTC Cell Counter + AnalyZer E L E C T R I C A L C U R R E N T EXCLUSION ECE BASED CELL ANALYSIS

2 ELECTRICAL CURRENT EXCLUSION CASY Model TTC Cell Counter + Analyzer R&D Easy and fast adaption to an unlimited number of appications combined with a non-invasive measurement principle are the key features making CASY Model TTC an indispensible tool for cell culture analysis in R&D. Different precision measuring capillaries in combination with cell specific setups allow to measure all types of mammalian cells as well as bacteria, yeast, algae, parasites, pollen and sperms. Flexible software tools for data evaluation allow scientists to follow the metabolic state of the cells during any type of cell culture experiment. P r o c e s s Development Improvement of cell culture processes is based on fast and accurate data on the quality of your cells. Besides cell concentration CASY Model TTC monitors cell viability, cell size and cell aggregation. In addition cell debris turned out to be an important quality control parameter in process control. With a measuring time of only 10 seconds for a single measurement different parameters affecting the state of your cells can be thouroughly tested in a short time. data can be directly compared from user to user, from day to day and from system to system. CASY Model TTC is especially recommended for the control of processes not bound to Trypan Blue. Product Description CASY Model TTC represents a fast and easy to use Cell Counter + Analyser system for the Total Quality Control of cell cultures with integrated data evaluation and complete documentation. Based on non-invasive Electrical Current Exclusion the system determines a standardized high resolution size distribution which is like a finger-print of the state of a cell culture. The basic parameters given are: Cell concentration Cell viability Cell volume Cell aggregation Cell debris Production Complete standarisation is an integral part of the CASY Model TTC based on embedded system technology, factory certified calibration and a built-in quality control device. The system conforms the full range of guidelines for GLP/GMP as well as 21 CFR Part 11 and therefore meets all requirements for the validation of cell culture processes. Measurement CASY Model TTC Measuring Results Example U937

3 ECE -BASED CELL ANALYSIS Benefits Electrical Current Exclusion (ECE ) is the only well accepted method for the dye-free determination of cell viability. Viability Measurement by Electrical Current Exclusion, ECE Together with the short measurement time and the low sample volume the CASY - Technology offers a unique combination of features for the quality control of cell cultures making it fast, easy to use, flexible and precise. Quality of Factory Certified Calibration Size Distribution of 10 µm Test Beads System Audit Trail and Error Audit Trail are the basis for the 21 CFR part 11 compliance of CASY Model TTC Simultaneous quantification of small debris particles, dead cells and viable cells besides huge aggregates. Accurate and reproducible results which can be directly compared from user to user, from day to day and from CASY system to CASY system based on the factory certified calibration. Non-invasive method of cell viability deter-mination avoiding the cytotoxic side effects of dyes. High precision measurement of cell volume. Correct quantification of even heavily aggregating cell samples. Low sample volume (5 100 µl). Low measuring time (10 sec). Fully qualified to be used in clean rooms. Low running cost per sample. Nearly maintenance-free. Calibration identical for all devices and stable for the entire service life. Compliant to all quality control regulations like GLP/GMP and 21 CFR 11. Comparison of Count and Volume Distribution of CHO Cells with an Aggregation Factor of 1.19

4 ELECTRICAL CURRENT EXCLUSION CASY Model TTC Cell Counter + Analyzer H i g h R e s o l u t i o n Size Distribution The high resolution size distribution offered by CASY -Technology ensures that the size of every individual cell or particle passing through the measuring pore can be detected. A clear-cut differentiation between cell debris, dead cells, viable cells and cell aggregates is provided, and even volume differences of a fraction of a femtoliter can be detected. Cell volume is a very sensitive parameter that can be used to detect changes in a cell culture. Therefore, a visualized finger-print of the metabolic state of the cell culture is provided. Typical CASY High Resolution Size Distribution Technology CASY Model TTC is based on the international accepted reference method for cell counting ISO In addition the system is equipped with an advanced approach of digital pulse processing called Pulse Area Anaysis. This technique is used to get the full information out of the signals created when a cell or a particle is passing the high precision measuring pore in a low voltage field. Each single object is scanned with a frequency of one million measurements per second resulting in up to several 100 multiple readings per cell. Thus the complete time course of the signals is taken into account to analyse your samples Pulse Area Anaysis provides a unique high resolution recording of the cell size distribution, an outstanding dynamic measurement range. It is the basis for the dye-free, non-invasive determination of cell viability. Pulse Area Analysis How it works

5 ECE -BASED CELL ANALYSIS Product Number CASY Model TTC Product No Data Management The evaluation program CASY process is an easy-to-use MS-Excel-based tool for the processing and representation of whole series of measurements. It is designed to match especially the needs in basic research and in process development. With CASY process you get a complete overview on up to 256 measurements assigned to several parallel experiments. The three main modules 2-D-Overlay, Mean and Diagram provide all functions to compare, evaluate and chart your results based on the measurement time or any external experimental parameter. Technical Data Measurement principle Electronic pulse area analysis with 1 Mio. measurements / sec. Viability determination Electrical Current Exclusion (ECE ) Dynamic range in volume > 1: in diameter > 1: 40 Measured size channels Displayed size channels Measurement range µm Resolution 1 in Typical analysis time 10 sec. Graphic User Interface of CASY process Software Typical sample volume µl Interfaces RS 232 (DB9), TCP/IP (RJ45), USB, PS/2, PCMCIA-Port for Flash-Card

6 ELECTRICAL CURRENT EXCLUSION CASY Model TTC Cell Counter + Analyzer Applications With the CASY Model TTC there are no limitations concerning the type of cell and the type of application. The system allows to follow each type of cell culture experiment and is recommended to be used for the quality control, the improvement and the longterm-monitoring of biological processes. Typical applications include: Counting of all types of cells Quality control of cell fractionation After fractionation of cells using different techniques such as centrifugation, magnetobeads, flow cytometry or counterflow centrifugal elutriation, fractions need to be checked with respect to yield, purity and state of the cells. The CASY Model TTC provides all parameters needed for quality control of the purified cell fractions. With a size range of 0.7 to 160 µm, nearly all types of cells can be measured. This includes bacteria, yeast cells, algae cells, protozoa and all types of mammalian cells. The CASY high resolution size distribution also provides important information about the metabolic state of the cells, in addition to counting cells. Determination of cytotoxic effects on cells Decrease in Cell Viability (% of Control) WST-8 Measuring of Cytotoxicity: IC50 - Determination Neutral Red Trypan Blue CASY-ECE 0,001 0,01 0, Concentration of Cytotoxic Agent (mm, log) In addition to cell concentration, cell viability and cell aggregation the CASY Model TTC provides precise information on changes in cell volume. Cell volume is a characteristic parameter that can be used to monitor the metabolic state of cells, control the process and define the correct time point for harvesting The sensitivity of any cytotoxicity assay depends on the efficiency of quantification of the reproductive integrity of cells (inhibition of cell proliferation) and the structural integrity of cells (cell death). Based on the high resolution size distribution, as well as Electrical Current Exclusion, CASY Model TTC determines both effects of toxic substances on cells independently.

7 ECE -BASED CELL ANALYSIS Monitoring of cell differentiation Cell differentiation is generally accompanied by considerable changes in cell volume. The CASY high resolution size distribution provides a finger print of the actual state of cell differentiation and can be used as a fast tool for following the process. Monitoring of virus infections of cells Application Support Baculovirus infections of insect cells are used to produce large amounts protein in infected cells. During infections, cell volume increases dramatically. Precise volume determination allows the user to monitor the process and calculate the degree of infection (MOI). Our application support team has 20 years of experience with all kinds of cell culture applications. A huge application data base helps to answer all questions coming up fast and allows to provide you with typical measurement examples from our comprehensive cell library. Based on this information our team is well prepared to assist you in establishing new applications and to develop adapted data evaluation solutions.

8 ELECTRICAL CURRENT EXCLUSION CASY Models TTC Cell Counter + Analyzer Consumables CASY ton CASY ton is a ready-to-use isotonic dilution liquid for cell cultures especially developed for use with CASY Cell Counter + Analyzer Systems. The particle purity of the liquid guarantees a low background to the measurements. CASY ton is manufactured according to the strict standards of the quality management system ISO 9000 ff. Properties CASY ton reduces aggregation of cells in the measuring cup and stabilizes the cells for the duration of the measurement time. The defined conductivity guarantees consistent measurements. Physiological ph and osmolarity ensure the preservation of cell size. Principle CASY supplies are especially designed for standardized CASY equipment. Only the exclusive and regular use of CASY supplies guarantees long-term consistency of measurement results, as well as long-term stability and optimal functionality of the equipment. Description Packaging CASY ton is available directly from innovatis AG in convenient 10 liter containers, including a plastic dosage tap. Shelf-life and Storage The best-before date is indicated on the product packaging. CASY ton can be stored at room temperature. CASY ton is an isotonic, particle-pure, odorless and colorless saline solution. Waste Disposal Waste must be disposed of in accordance with local cell culture laboratory regulations. Product Number Product No

9 ECE -BASED CELL ANALYSIS ceramus Dispenser 2 10 ml The ceramus Dispenser, made by Hirschmann Laborgeräte, Eberstadt, Germany, is the digitally adjustable top bottle dispenser for the dosage of liquids that innovatis recommends for use with CASY ton. Principle Packaging The ceramus Dispenser is delivered with an individual certificate of performance. The package contains several adapters for different types of bottle necks. One of the adapters fits the inlet of the CASY ton container. A manual explains all of the necessary aspects of installation, handling and cleaning. The ceramus Dispenser allows CASY users to easily adjust the dose of CASY ton, in steps of 0.25 ml. The delivered discharge tube unit is equipped with a Luer lock adapter. Therefore it is possible to rinse each portion of CASY ton through a particle filter with a defined pore size compatible to the capillary used with the CASY system. Additional Features The ceramus Dispenser can be autoclaved (refer to the manual for instructions). The recirculation valve allows for priming in order to avoid the accumulation of air bubbles in the CASY ton. Properties The permanent use of the ceramus dispenser allows for a highly reproducible adjustment of the liquid volume of CASY ton needed for every measurement. In combination with a connected particle filter, this guarantees optimum quality in purity and volume precision. Operation Conditions The ceramus dispenser is suitable for operating temperatures between +4 C and +50 C. Product Number Product No

10 ELECTRICAL CURRENT EXCLUSION CASY Models TTC Cell Counter + Analyzer Consumables CASY clean CASY clean is a ready-to-use cleaner for the CASY Cell Counter + Analyzer Systems. Principle CASY supplies are especially designed for standardized CASY equipment. Only the exclusive and regular use of CASY supplies guarantees long-term consistency of measurement results, as well as long-term stability and optimal functionality of the equipment. Properties Regular use protects the device and measurement capillaries from biological deposits. Through its emulsifying effect, CASY clean prevents blockages and encrustations of the internal liquid container system and measurement capillaries. Packaging CASY clean is available directly from innovatis AG as a package of 3 x 500 ml bottles. Description CASY clean is a foam-free, alkaline, odorless and colorless cleaning solution for the system and measurement capillaries. CASY clean is non-toxic and non-flammable. Shelf-life and Storage The best-before date is indicated on the product packaging. CASY clean should be stored in a cool, dry place and should be protected from light. Waste Disposal Waste must be disposed of in accordance with local cell culture laboratory regulations. Product Number Product No

11 ECE -BASED CELL ANALYSIS CASY blue CASY blue creates a standardized control sample of dead cells. Principle CASY supplies are especially designed for use with standardized CASY equipment. Only the use of CASY blue guarantees a standardized viability determination with the CASY Cell Counter + Analyser Systems of innovatis AG. Description CASY blue is a ready-to-use, blue, alcoholic dilution for the determination of the cell matrix volume. CASY blue acts highly specific and is used together with the CASY Cell Counter + Analyzer Systems of innovatis AG for establishing cell specific setups for viability determination and for the ongoing quality control of cells. Utilization Use CASY blue on a regular basis to check the cursor settings defined for viability determination by ECE and for the on-going quality control of your cells to guarantee cell identity. CASY blue covers all TQC requirements of cell culture labs in research and production. Please find the detailed standard operating procedure (SOP) for using the CASY blue enclosed. Packaging CASY blue is available directly from innovatis as a packaging unit of 4 x 30 ml bottles. Shelf-life and Storage The best-before date is indicated on the product packaging. CASY blue should be stored in a cool, dry place, protected from light. Close bottle tightly after use. Waste Disposal Waste must be disposed of in accordance with local cell culture laboratory regulations. Product Number Product No

12 ELECTRICAL CURRENT EXCLUSION CASY Models TTC Cell Counter + Analyzer Consumables CASY cups CASY cups are measurement cups with lids designed for use with CASY Cell Counter + Analyzer Systems. The material properties, size and volume of CASY cups are optimally adapted for cell culture sample preparation. CASY cups are disposable and designed for single use. Repeated use can have an effect on measurement results. Principle CASY supplies are especially designed for use with standardized CASY equipment. Only the exclusive and regular use of CASY supplies guarantees long-term consistency of measurement results, as well as long-term stability and optimal functionality of the equipment. Description Packaging CASY cups with lids are available directly from innovatis in packages of 1,800 cups. Shelf-life and Storage CASY cups must be stored at room temperature in a dry, dust-free location. The shelf-life is unlimited. CASY cups cups measure 22 x 65 mm with a filling volume of 15 ml. They are made of Polypropylene (PPN), and are transparent and milky in color. CASY cups are particle-pure and biologically inert. The material prevents cells from adhering to the cup walls. The cups are heat-resistant up to 80 C and slowly inflammable. CASY rack for 24 pcs CASY cups. Waste Disposal Waste must be disposed of in accordance with local cell culture laboratory regulations. Product Number CASY cups CASY rack

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