Required Textbook: Microbiology: An Introduction 10th Ed by Tortora, Funke, and Case

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1 Course Syllabus Derek Boyer Biology 210 Fall 2009 Office Phone: (714) Office: Science Hall 131 or C&L 108 (Lab) Hrs: M/W 1:00-2:00pm in C&L 108 (Lab) T/Th 11:00-12:00pm in Science Hall 131 Course Description: Biol 210 is a 5 unit college transferable course that introduces you to the microbial world a world of viruses, bacteria, molds, yeasts, protozoa, algae, and helminths. Emphasis will be placed on microbial structure, metabolic activities, microbial genetics, hostparasite relationships, host resistance, and the control of microbes. Lab activities will include isolation and cultivation of bacteria, studies of bacterial isolates, understanding bacterial population dynamics, control of microbial growth, mycology, parasitology, and virology. Required Textbook: Microbiology: An Introduction 10th Ed by Tortora, Funke, and Case Required Lab Manual: Laboratory Theory and Application, 2 nd Ed. by Leboffe and Pierce Derek Boyer s webpage: has links to supplementary materials and a copy of this course syllabus. Student Learning Outcomes: 1. Demonstrate appropriate laboratory skills and techniques related to the isolation, staining, identification, assessment of metabolism, and control of microorganisms. 2. Evaluate the physical and chemical methods used in the control of microorganisms and apply this understanding to the prevention and control of infectious diseases when given a scenario. 3. Discuss and diagram the role of the immune system in maintaining homeostasis, challenging infections, and fighting cancer. Grading: Your course grade is based on a percent of accumulated points. These points are acquired by examinations, quizzes, lab assignments, and lab reports. The grading scale is: A = % (4) Lecture exams 125 pts ea = 500 B = 80-89% (5) Lecture quizzes 20 pts ea = 80 (1 dropped) C = 70-79% (1) Written assignment 20 pts = 20 D = 60-69% (2) Lab exams 80 pts ea = 160 F = Below 60% (1) Lab final 100 pts = 100 (5) Lab quizzes 10 pts ea = 40 (1 dropped) Lab assignments 70 pts total = 70 Lab unknown 30 pts total = _ pts total Make-up exams: Only one make-up will be allowed during the semester. Based on an excused absence you must contact me the day of the exam. Make-ups are essay exams. There are no make-up quizzes. Academic dishonesty on exams or quizzes is cause for an instructor drop (See the campus policy regarding academic dishonesty). Do not give or accept help during an exam! Lecture has a total of 600 points which come from 4 exams, 5 quizzes (lowest quiz score dropped), and 1 written assignment.

2 Points for lab will total 400 and will include 2 exams and a final, 5 quizzes (lowest quiz score dropped), assignments, and an unknown project. Late lab assignments will not be accepted or will be given a penalty depending on the time frame of the assignment. Please note that all quizzes (lecture and lab) are given at the beginning of the period and take about 20 minutes. If you are late you will not be given extra time to complete your quiz. There are no make-up quizzes.

3 Week Date General Microbiology Lecture Topics Assigned Chapters 1 T Sept 1 Introduction 1 R Sept 3 History & Nomenclature 1 2 T Sept 8 Chemistry 2 R Sept 10 Chemistry 2 3 T Sept 15 Lecture Quiz 1; Prokaryotes & Eukaryotes 4 R Sept 17 Prokaryotes & Eukaryotes 4 4 T Sept 22 Microbial Metabolism 5 R Sept 24 LECTURE EXAM 1 1, 2, 4 F Sept 26 ****Last Day to Withdraw to Avoid a W **** 5 T Sept 29 Microbial Metabolism 5 R Oct 1 Microbial Metabolism 5 6 T Oct 6 Microbial Growth 6 R Oct 8 Control and Antimicrobial Drugs 7, 20 7 T Oct 13 Lecture Quiz 2; Control and Antimicrobial Drugs 7, 20 R Oct 15 Microbial Genetics 8 8 T Oct 20 Lecture Quiz 3; Microbial Genetics 8 R Oct 22 Biotechnology and Recombinant DNA 9 9 T Oct 27 Eukaryotes: Fungi, Algae, and Protozoa 12 R Oct 29 LECTURE EXAM 2 5, 6, 7, 8, 9, T Nov 3 Virology 13 R Nov 5 Virology T Nov 10 Lecture Quiz 4; Microbial Pathogenesis 15 R Nov 12 Immunology 16, T Nov 17 Immunology / Vaccines 17, 18 R Nov 19 Epidemiology 14 Microbial Diseases of the Skin and Eyes 21 F Nov 21 ****Last Day to Drop**** 13 T Nov 24 LECTURE EXAM 3 12, 13, R Nov 26 Holiday Thanksgiving, No Lecture 14 T Dec 1 Microbial Diseases of the Respiratory System 24 R Dec 3 Microbial Diseases of the Digestive System T Dec 8 Lecture Quiz 5 Microbial Diseases of the Urinary and Reproductive 26 Systems R Dec 10 Microbial Diseases of the Cardiovascular and Lymphatic 23 Systems 16 T Dec 15 Microbial Diseases of the Nervous System 22 R Dec 17 LECTURE EXAM 4 14, 21 26

4 WK Date Projected Lab Topics Lab Exercises 1 M/T Aug 31/1 Orientation; Lab safety; Microscopy handout; 3-1 View slides of colored threads, blood smears, and the letter e Set up environmental plates (Incubate at 37 C) 2-1 W/R Sept 2/3 Wet mounts of cheek cells; Aseptic technique, Simple stain pg. 63 (top); 1-2, 3-4 Check and discuss environmental plates (Part A - Bacteria) 2-1; 2-2 **Continue growing environmental plates at room temp. 2 M/T Sept 7/8 Holiday - Labor Day, No Lab W/R Sept 9/10 Isolation of bacteria (Part I): Streak plate #1 1-3 Negative Stain; Gram stain 3-5; M/T Sept 14/15 Check Streak Plate #1 and set up Streak Plate #2 1-3 Yeast and filamentous fungi: Tape mounts; Slide cultures (demo 11-1 including wet mount of yeast) Check and discuss environmental plates (Part B - Fungi) 2-1; 2-2 W/R Sept 16/17 Quiz 1; Isolation of bacteria (Part II): Standard plate count 6-1 Endospore stain; Check Streak plate #2 3-9 Carbohydrate Catabolism/Fermentation; Respiration 4 M/T Sept 21/22 OF-glucose; PR-broth; Capsule stain; Acid-fast stain 5-1; 5-2; 3-8; 3-7 Read results for Standard plate count W/R Sept 23/24 IMViC (Indole/MRVP/Citrate) 5-20, 5-4, 5-8 Starch hydrolysis; ONPG 5-13; 5-14 Read results for OF-glucose, PR-broth; Preparation of media (demo) M/T Sept 28/29 Quiz 2; Read results for IMViC, Starch hydrolysis, and ONPG W/R Sept 30/1 Exam 1 Protein Catabolism 6 M/T Oct 5/6 Decarboxylase; Phenylalanine; Urease; Casease 5-10; 5-11; 5-15; 5-16 W/R Oct 7/8 Gelatinase; Motility/Indole/Ornithine (MIO - similar to SIM) 5-17; 5-20 & handout Read results for Phenylalanine, Urease, Casease 7 M/T Oct 12/13 Respiration Anaerobic respiration: Anaerobic jar, Nitrate reduction 2-9, 5-7 Aerobic respiration: Catalase, Oxidase 5-5, 5-6 Read results for Decarboxylase, Gelatinase, MIO W/R Oct 14/15 Set up Temperature exp.; ph demo; Osmotic pressure demo 2-10; 2-11; 2-12 Thioglycollate broth demo 2-8 Read results for the Anaerobic jar and Nitrate reduction **Remember antiseptics/disinfectants for next class 8 M/T Oct 19/20 Control of Microbial Growth Quiz 3; UV light; Disinfectants and antiseptics 2-13, 9-2; 2-14 Antimicrobial susceptibility test (Kirby-Bauer method) 7-3 Read results from the Temperature experiment W/R Oct 21/22 View Protozoan slides 11-2 Read UV, Disinfectant/antiseptic, and Kirby-Bauer plates **Remember water samples for next class

5 WK Date Projected Lab Topics Lab Exercises 9 M/T Oct 26/27 Microbes in Food/Water Set up Water Analysis: Membrane filter technique, LTB tubes 8-1 & 4-4, 8-2 Parasite video; View Protozoan slides (continued) 11-2 Unknown project begins Streak onto TSA & MacConkey s agar 7-6, 4-7 W/R Oct 28/29 Water Analysis (continued): BGLB tubes 8-2 Epidemiology experiment; Finish protozoan slides 7-5; 11-2 Read results for Membrane filters and LTB tubes Unknown projects: Look for colony isolation and set up NA slants, Gram stain each colony type (if time permits) 10 M/T Nov 2/3 Quiz 4; Read results for Epidemiology and BGLB tubes 7-5; 8-2 Review W/R Nov 4/5 Exam 2 11 M/T Nov 9/10 Virus video; Set up Plaque Assay 6-4 Work on unknown projects W/R Nov 11/12 Holiday - Veteran s Day, No Lab 12 M/T Nov 16/17 Bacteria of the Skin, Respiratory Tract, and Mouth Nasal swab on MSA; Throat swab on blood agar 4-1; 5-26 Set up CAMP tests with Bacitracin test (A-disk), pg. 259; 7-7 Optochin (O-disk); Bile Esculin 7-7; 5-12 Read results for Plaque Assay W/R Nov 18/19 Nasal swab isolate: Coagulase test, Gram stain 5-27; 3-6 Throat swab isolate: Gram stain and identification handout Read CAMP tests; View hemolysis patterns demo 5-26 Bacteria of the mouth (discussion only) 13 M/T Nov 23/24 Blood smear differentials; Blood typing 10-1; 10-5 View Helminth slides 11-3 Read results for nasal/throat isolates W/R Nov 25/26 Holiday - Thanksgiving, No Lab 14 M/T Nov 30/1 Bacteria of the Gastrointestinal/Urogenital Tract Mock fecal sample: Streak on MacConkey s and Nutrient agar 4-7 Triple Sugar Iron (TSI) Agar 5-21 Mock Urine Sample: Bacturcult rapid test handout View Helminth Slides (continued) 11-3 W/R Dec 2/3 Quiz 5; Analyze Mock fecal plates: Gram stain, Oxidase 3-6; 5-6 Read results for the Bacturcult and TSI 15 M/T Dec 7/8 Review; Finish/work on unknown projects W/R Dec 9/10 Final Exam, comprehensive 16 M/T Dec 14/15 Clean up the lab; Finish all lecture material W/R Dec 16/17 No Lab; Lecture Final Exam

6 SAFETY ISSUES IN THE BIOLOGY 210 MICROBIOLOGY LABORATORY 1. Do not come to lab late, new procedures (which often involve safety) are discussed at the beginning of lab. 2. Wear a lab coat or smock at all times in the lab. Store your coat/smock on the coat rack or in an assigned drawer. 3. Be aware of Bunsen burner flames. Turn off burners when not in use. Secure long hair to prevent accidental exposure to open flames. Report burns and other injuries to your lab instructor immediately. 4. Use the eye wash station immediately, should any reagent accidentally get into your eyes. 5. Do not eat, drink, or store, food/drinks in the lab. 6. Place contaminated items in autoclavable containers for disposal. Do not mix glass with plastic items. 7. Use the biohazard bag to dispose of biological waste DO NOT use the trash basket to dispose of biological waste. 8. Remove old cultures from the refrigerator or incubator and place them in the biohazard bag for disposal. Remove labels from glassware when disposing. Do not remove cultures from the lab or place them in your lab drawer. 9. Report accidental spills of cultures to your instructor immediately. Do not attempt to pick up broken glass yourself. 10. Try to avoid bringing your hands to your mouth while in lab. 11. Wash your hands with soap and water before leaving lab. 12 Cover cuts on exposed skin with a sterile bandage. 13. Disinfect your desktop at the beginning and end of lab. 14. Inform your lab instructor if you are pregnant or if you are immunosuppressed as some lab exercises may pose unique problems in such cases. 15. Wear goggles when using reagents and when near ultraviolet lights. 16 Use paper towels soaked with disinfectant when pouring plates to avoid aerosols. 17. Use proper aseptic technique when making culture transfers. I,, have read the above safety issues. I understand them and will endeavor to follow these precautions to the best of my ability.