Product Datasheet. Collagen I alpha 1 Antibody (COL-1) NB Unit Size: 0.1 ml

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1 Product Datasheet Collagen I alpha 1 Antibody (COL-1) NB Unit Size: 0.1 ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 3 Publications: 19 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 12/11/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Collagen I alpha 1 Antibody (COL-1) Product Information Unit Size Concentration Storage Clonality Clone Preservative Isotype Purity Buffer Target Molecular Weight Product Description Host 0.1 ml This product is unpurified. The exact concentration of antibody is not quantifiable. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Monoclonal COL-1 15mM Sodium Azide IgG1 Unpurified Ascites 139 kda Mouse Gene ID 1277 Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen Product Application Details Applications COL1A1 Human, Mouse, Rat, Porcine, Bovine, Equine, Feline, Mammal, Rabbit Also reported reactivity in deer. Mouse reactivity reported in scientific literature (PMID: ). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Recognizes the native (helical) form of collagen type I using ELISA and dot-blot. Does not react with the thermally-denatured molecule. The shows no crossreactivity with collagen types II, III, IV, V, VI, VII, IX, X and XI. Full length bovine native protein (purified). Western Blot, Dot Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Immunocytochemistry Recommended Dilutions Western Blot 1:100-1:2000, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence, Immunohistochemistry- Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:2000, Dot Blot 1:100-1:2000, Immunocytochemistry Application Notes Page 1 of 6 v.20.1 Updated 12/11/2018 The predicted molecular weight in western blot is 95 kda. In immunohistochemical testing of acetone-fixed or unfixed frozen sections, strong staining of connective tissue fibers is seen. This antibody has been used in IHC as reported in the literature (PMID: ) including IHC-Paraffin (PMID ) and ICC (PMID ).

3 Images Western Blot: Collagen I alpha 1 Antibody (COL-1) [NB ] - Analysis of collagen 1. Lane 1: Recombinant human collagen 1 (3 ug). Lane 2: Pig skin whole cell lysate (20 ug) extracted with Laemmli buffer. Page 2 of 6 v.20.1 Updated 12/11/2018 Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB ] - Staining of acetone-fixed, frozen sections of pig tongue at a dilution of 1:4000 Immunocytochemistry: Collagen I alpha 1 Antibody (COL-1) [NB ] - Collagen I alpha1 labeled pig trabecular meshwork cells indirectly visualized with Alexa Fluor 488 conjugated secondary antibody. Image from verified customer review. Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB ] - Analysis of an FFPE ligament section. Enzymatic antigen retrieval and blocking using 1% BSA for 30 min at 20C was performed. Sample was incubated for 1 hour with primary antibody at a dilution of 1:400 at 20C.

4 Immunohistochemistry-Frozen: Collagen I alpha 1 Antibody (COL-1) [NB ] - Staining of acetone-fixed, frozen sections of human tongue at a dilution of 1:2000. Page 3 of 6 v.20.1 Updated 12/11/2018 Immunohistochemistry: Collagen I alpha 1 Antibody (COL-1) [NB ] - Analysis of formalin fixed frozen porcine skin tissue sections. Blocking: 5% serum for 1 hr at 20C. Sample was incubated for 16 hours at 4C and used at a dilution of 1:500. Immunocytochemistry: Collagen I alpha 1 Antibody (COL-1) [NB ] - Analysis of Collagen I alpha 1 in 4% PFA fixed equine tenocytes using anti-collagen I alpha 1 antibody. Image from verified customer review.

5 Publications Liu G Y, Agarwal R, Ko K R et al. Templated Assembly of Collagen Fibers Directs Cell Growth in 2D and 3D. Scientific Reports 2017 [PMID: ] (IHC, Mouse) Du Y, Liu H, Yang Q et al. Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. Biomaterials. Aug :00AM [PMID: ] (IHC, Rabbit) Costa D, Vene R, Benelli R et al. Targeting the Epidermal Growth Factor Receptor Can Counteract the Inhibition of Natural Killer Cell Function Exerted by Colorectal Tumor-Associated Fibroblasts Front Immunol May :00AM [PMID: ] (Human) Wegner K, Keikhosravi A, Eliceiri K et al. Fluorescence of Picrosirius Red Multiplexed With Immunohistochemistry for the Quantitative Assessment of Collagen in Tissue Sections. J Histochem Cytochem 2017 Jul 10 [PMID: ] (IHC-P, Mouse) Kuzan A, Chwilkowska A, Maksymowicz K et al. Advanced glycation end products as a source of artifacts in immunoenzymatic methods Glycoconj. J Jan 05 [PMID: ] (ELISA, Human) Ramani K, Tomasi ML, Berlind J et al. Role of A-Kinase Anchoring Protein Phosphorylation in Alcohol-Induced Liver Injury and Hepatic Stellate Cell Activation Am. J. Pathol Jan 02 [PMID: ] (Human) da Silva IR, Tiveron LCRDC, da Silva MV et al. In Situ Cytokine Expression and Morphometric Evaluation of Total Collagen and Collagens Type I and Type III in Keloid Scars. Mediators Inflamm Jun 22 [PMID: ] (IHC, Human) Loy C, Laine A, Mantovani D. Rotation-based technique for the rapid densification of tubular collagen gel scaffolds. Biotechnol J 2016 Dec [PMID: ] Bono N, Meghezi S, Soncini M et al. A Dual-Mode Bioreactor System for Tissue Engineered Vascular Models. Ann Biomed Eng Feb 21 [PMID: ] Karti O, Zengin MO, Cinar E et al. Effect of 1- and 6-Hour-Delayed Corneal Collagen Cross-Linking on Corneal Healing in a Rabbit Alkali-Burn Model: Clinical and Histological Observations. Cornea. Oct :00AM [PMID: ] Sa G, Xiong X, Ren J et al. Expression of fibrosis-related molecules in the oral mucosa of six animal species: A reference for selecting animal models. Eur J Pharm Sci Oct :00AM [PMID: ] (IHC, ICC/IF, Human) Cheng MQ, Wahafu T, Jiang GF et al. A novel open-porous magnesium scaffold with controllable microstructures and properties for bone regeneration. Sci Rep Apr 13 [PMID: ] (IHC-P, Human) More publications at Page 4 of 6 v.20.1 Updated 12/11/2018

6 Procedures Immunohistochemistry Protocol for Collagen I Antibody (NB ) Sample Human Tissue sections (Normal Skin and Basal Cell Carcinoma/Solar Elastos) Application Immunohistochemistry (Formalin-fixed paraffin-embedded sections) Blocking step: None Antigen retrieval step: Heat mediated Incubation time: 30 minute(s) Dilution: 1/800 Additional notes The tissue was FFPE (formalin fixed, paraffin embedded tissue sectioned at 3-4 microns). The antigen retreival method was a heat-induced antigen retreival (HEIR) using a vegetable steamer. Sections were incubated in the steamer for 20 minutes in 1 mm EDTA buffer, ph 8.0. Sections were then rinsed in H2O and TBS buffer prior to starting the staining protocol. The staining appeared to be very specific and robust, with very little to no non-specific background staining. Staining Summary & Notes: 1mM EDTA Buffer, ph 8.0, HIER for 20 minutes in steamer 10 minute blocking in 3% Peroxide 30 minutes in Primary RT 30 minutes in Secondary RT 10 minutes in DAB+ RT Water Rinse Counterstain for 6 minutes in Mayer's Hematoxylin RT Secondary antibody Name: DakoCytomation Envision+ Anti-Mouse/HRP (RTU) Conjugation: HRP Dilution: 1/1 Page 5 of 6 v.20.1 Updated 12/11/2018

7 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP NBP Goat anti-mouse IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG1 Isotype Control (MG1) Collagen I alpha 1 Native Protein Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: