Aquatic biomass parallel session. Moderator: Paloma MALLORQUIN BBI JU

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1 Aquatic biomass parallel session Moderator: Paloma MALLORQUIN BBI JU

2 Types of actions

3 Research & Innovation Actions (RIAs) The budget line approach All proposals submitted under the same budget line compete for the same budget For one topic: 0, 1 or more projects can be funded Main advantage of budget line approach: only the best (highest-scoring) proposals are funded Call 2016 Topic R9: - Exploiting algae and other aquatic biomass for production of molecules for pharma, nutraceuticals, food additives and cosmetic applications 4 RIA presentations (ABACUS, BIOSEA, VALUEMAG, MAGNIFICENT): all funded under the same topic

4 Research & Innovation Actions (RIAs) Horizon 2020 definition vs. BBI JU Activities aiming to establish new knowledge and/or to explore the feasibility of a new or improved technology, product, process, service or solution. Translated to bio-based industries: tackling a challenge in part of or in the whole value chain (biomass processing end products) Aquatic biomass sector High added value products Achieve cost-competitive concepts: reduce the costs of the extraction and conversion processes

5 ABACUS

6 Grant Agreement N A joint European initiative to foster algae as a biomass applied to the production of added value compounds abacus aims at a business-oriented and technology-driven development of a new algal biorefinery, thereby bringing to the market competitive and innovative algaebased ingredients for high-end applications, spanning from algal terpenes for fragrances to long-chain terpenoids (carotenoids) for nutraceuticals and cosmetic actives. Terpenoids can be found in photosynthetic microorganisms like plants or algae. They represent a renewable alternative to petroleum-derived fuel and building blocks of synthetic biopolymers as well as high value compounds for fragrances, cosmetics and nutraceuticals

7 Grant Agreement N

8 Grant Agreement N Specific objectives Selection and biological engineering of microalgal strains for oriented photosynthesis of terpenoids Technological improvement and automation of algae production systems to enhance biomass yields and photosynthesis of terpenoids Optimize downstream processing to reduce OPEX and to secure societal and environmental acceptability Assess applicability of targeted ingredients considering cosmetic and nutraceutical applications Market development of formulated products based on new algae-based ingredients with structuration of unprecedented value chains

9 BIOSEA

10 7/12/2017 Innovative cost-effective technology for maximizing aquatic biomass-based molecules for food, feed and cosmetic applications BIOSEA PROJECT - GENERAL PRESENTATION Topic: BBI-2016-R09 Call Identifier: H2020-BBI-JTI-2016 Type of Action: BBI-RIA This project has received funding from the Bio Based Industries Joint Undertaking under the European Union s Horizon 2020 Research and Innovation Programme under grant agreement No

11 7/12/2017 OVERALL OBJECTIVE OF THE PROJECT BIOSEA Is aimed at developing and validating of innovative, competitive and cost-effective upstream and downstream processes for cultivation of: 2 microalgae (Spirulina platensis and Isochrysis galbana) and 2 macroalgae (Ulva intestinalis and Saccharina latisima) to produce and extract high value active principles at low cost (>55%) to be used in: food, feed and cosmetic/personal care high-value added products will contribute in reducing Europe s feedstock imports, diminishing the pressure of land resources and opening new markets and business opportunities.

12 7/12/2017 OVERALL OBJECTIVE OF THE PROJECT Active compounds PROTEINS FATTY ACIDS CARBOHYDRATE S CAROTENOIDS LIPIDS PHYCOBILIPROT EINS Sectors to be employed FOOD FEED COSMETICS

13 7/12/2017 OVERALL OBJECTIVE OF THE PROJECT

14 7/12/ months 1 june may partners from 5 different countries Total budget Requested funding Industrial contribution ,50 (42 % of the total budget) Project duration Partners BIOSEA budget 14

15 MAGNIFICENT

16 Maria Barbosa, Hans Reith, René Wijffels Maria J. Barbosa

17 Cultivation and Harvesting Sustainable Ingredients production End-product formulation & Applications

18 Microalgae: Sustainable Ingredients & New business opportunities DEVELOPMENT OF NEW COMMERCIAL & SUSTAINABLE PRODUCTS WITH ALGAE INSIDE REPLACEMENT OF MARINE OIL FROM KRILL AND COPEPODS IN LARVAL FEEDS FOR EU MARINE SPECIES. REPLACEMENT OF FISH MEAL BY RESIDUAL BIOMASS RESULTING FROM BIOREFINERY DEVELOPMENT AND TESTING OF PETFOOD WITH MICROALGAE INGREDIENTS DEVELOPMENT AND TESTING OF NEW PROTOTYPE COSMECEUTICALS NEW OXIDANTS FOR THE FOOD INDUSTRY DEVELOPMENT OF ECONOMIC VIABLE PRODUCTION OF ALGAE BY INCREASING PRODUCT YIELD AND REDUCED OPERATIONAL COSTS BUSINESS PLAN FOR MARKET EXPANSION BASED ON THE INGREDIENTS PRODUCED

19 VALUEMAG

20 Developing a photobioreactor system based on magnetics nanoparticles and a magnetic cone Optimizing cultivation parameters Holistic production of food from microalgae biomass Presenting Angelo Ferraro, PhD, NTUA team BBI-JU Stakeholder Forum, Brussels 6-7 Dec. 2017

21 VALUEMAG proposes a revolutionary technology for microalgae industry in order to produce and purify high-value biomolecules in an affordable and environmentally-friendly manner This project has received funding from the Bio Based Industries Joint Undertaking grant no

22 Key aspects of Valuemag challenges Cultivation Extraction of valuable molecules Magnetic cell immobilization Super Critical Fluid (CO 2 ) extraction Cell division Electroporation Magnetically modified cell Magnetic nanoparticles accumulate at the cell-side facing the magnetic surface and generate enough magnetic moment to pull each parent cell towards the magnetic surface. A thin (1 mm) film of water covers cells New-generated non-magnetic cells are collected. Harvesting can be done continuously or periodically and the medium re-used Magnetic Particles (MP) -The presence of impurities is avoided thanks to the total recovery of gaseous CO2, -The low critical temperature of CO2 reduces the degradation of thermoslabile molecules. Selective Magnetic Separation -Functionalized MP are mixed with a complex blend -They specifically bind only the target molecule -MP and target molecule are retained by magnetic force -Impurity eliminated

23 MACROCASCADE

24 Challenges identified Robust seaweed biomass supply (stable and sufficient biomass availability) Cost-efficient configuration for seaweed conditioning, pre-treatment and storage (e.g. ensilation) Robust extraction and separation technologies for seaweed fractionation into raw fractions rich in alginate, fucoidan, mannitol, laminarin and proteins Efficient enzymatic refining of seaweed-derived raw fractions (laminarin, fucoidan, alginate) into high value added products Integrated approach for sustainability assessment of entire value chain

25 Objectives illustrated

26 Impact & IPR innovative biorefinery processes Ability to predict seasonal and regional variation in growth Utilizing crunching and screw pressing for cost effective conditioning of biomass Patentable feed and food product based on a mixture of rapeseed and macroalage Proof of concept for scalable and sustainable extraction/separation methods for the production of multiple products from brown seaweed or seaweed residues Develop a variety of efficient and robust carbohydrate active enzymes with a range of specificities for conversion of laminarin polysaccharides into branched immunomodulating β-glucan oligosaccharides, alginate into unsaturated uronic acids for chemical industry, fucoidan into bioactive fucose oligosaccharides and conversion of polysaccharides from red algae into prebiotic oligosaccharides. Techno-economic evaluation of the cascading platform Turnkey solutions for EU wide replication on up/downstream

27 Acknowledgement This presentation is part of the Macro Cascade project. This project has received funding from the Bio-Based Industries Joint Undertaking under the European Union Horizon 2020 research and innovation programme under grant agreement No

28 Aquatic parallel session Moderator: Paloma MALLORQUIN BBI JU