BIOTECHNOLOGY P.G DIPLOMA Duration - 1 year MODULE I o INTRODUCTION TO GENERAL MICROBIOLOGY

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1 BIOTECHNOLOGY P.G DIPLOMA Duration - 1 year MODULE I o INTRODUCTION TO GENERAL MICROBIOLOGY o BASIC CONCEPTS Sterilization techniques Microbiological culture media and its importance Isolation of microorganisms from soil air and water Pure culture preparation Staining techniques o BIOCHEMICAL TESTS o GROWTH CURVE o INDUCTION OF MUTATIONS Physical mutagenesis Chemical mutagenesis o MUTATIONAL STUDIES BY REPLICA PLATING o BACTERIAL CONJUGATION o SCREENING FOR DESIRED ORGANISMS o ISOLATION AND IDENTIFICATION OF FUNGI o PRODUCTION OF SECONDARY METABOLITES

2 o ANALYSIS OF METABOLITES Chromatography Solvent extraction o ANTIBIOTIC ASSAY o TITRIMETRIC ESTIMATION OF ORGANIC ACID o SPECTROPHOTOMETRIC ESTIMATION OF ENZYME PROTEIN o MICROBIAL ANALYSIS OF MILK o WATER ANALYSIS MODULE II o INTRODUCTION TO INDUSTRIAL MICROBIOLOGY o BASIC CONCEPTS Sterilization of fermentation equipment Fermentation systems Steps in fermentation Designing of production media Development and transfer of seed o SCREENING OF DESIRED ORGANISMS o TYPES OF FERMENTATIONS Solid state fermentation

3 Submerged fermentation o DOWN STREAM PROCESSING o CELL SEPERATION TECHNIQUES o EXTRACTION OF DESIRED PRODUCTS o PURIFICATION OF PRODUCTS o ANALYSIS OF METABOLITES OR PRODUCTS Chromatography Spectrophotometric determination Titrimetric estimation Electrophoresis o WATER ANALYSIS o ENZYME PRODUCTION Solid sate fermentation Submerged fermentation o ENZYME PURIFICATION TECHNIQUES Precipitation methods o CHROMATOGRAPHY o ENZYME ASSAY o QUANTITATIVE ESTIMATION OF PROTEINS

4 o ENZYME KINETICS ph Temperature Substrate concentration Activators Inhibitors o ELECTROPHORESIS TECHNIQUES SDS-PAGE Native PAGE Zymography o IMMOBILIZATION ENZYMES MODULE III RECOMBINANT DNA TECHNOLOGY FUNDAMENTALS o Isolation of Genomic DNA o Isolation of Total RNA o Quantitative and Qualitative analysis of Nucleic acids o Transformation o Isolation of Plasmid DNA

5 o Agarose Gel Electrophoresis o Restriction Digestion o PCR Techniques MODULE IV INTRODUCTION TO IMMUNOLOGY AGGLUTINATION REACTIONS o Blood grouping and Rh typing o Rapid plasma reagin test (VDRL) o Pregnancy test GEL DIFFUSION TECHNIQUES o Ouchterlony Double Diffusion (ODD) o Single Radial Immuno Diffusion (RID) IMMUNO ELECTROPHORESIS o Counter Current Electrophoresis (CCE) o Rocket Immuno Electrophoresis (RIE) ISOLATION OF IgG FROM BLOOD PURIFICATION OF IgG o Salt precipitation o Chromatography

6 o Quantitative estimation of proteins SDS PAGE SEPERATION OF LYMPHOCYTES ELISA (STRIP TEST) RAISING OF POLYCLONAL ANTIBODIES MONOCLONAL ANTIBODY PRODUCTION MODULE V INTRODUCTION TO MEDICAL BIOTECHNOLOGY BASIC CONCEPTS ISOLATION OF PATHOGENS BIOCHEMICAL TESTS (specialized test if any) ANTIBIOTIC SENSITIVITY DIAGNOSTIC TESTS VDRL WIDAL o C - reactive protein (CRP) o Rapid Plasma Reagin (RPR) REVIEW OF ANALYSIS

7 REPORT PREPARATION BIOCHEMICAL TESTS o Blood grouping and Rh typing o Hemoglobin test o Pregnancy test URINE ANALYSIS o Detection of urine protein o Detection of urine glucose o Detection of ketone bodies in urine sample WBC Count MIC and MLC INTRODUCTION TO BIOINFORMATICS