A Circular Economy Strategy: From mushroom compost to low-cost biopesticides. Biopesticides 2017 Madrid, June 8th 2017

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1 A Circular Economy Strategy: From mushroom compost to low-cost biopesticides Biopesticides 2017 Madrid, June 8th 2017

2 í n d i c e 1. The BIOrescue project 2. CENER Biomass Department Capabilities

3 1. The BIOrescue project

01 The BIOrescue project CHALLENGES Each year, over 3 million tonnes of mushroom compost is generated by mushroom production, thus creating significant economic and logistical problems for Europe s

4 01 The BIOrescue project CHALLENGES Each year, over 3 million tonnes of mushroom compost is generated by mushroom production, thus creating significant economic and logistical problems for Europe s farmers. Mushroom compost, prepared solely for growing mushrooms, is only suitable for one to three harvests; The compost is currently disposed of, even though it contains valuable components; The mushroom industry lacks adapted technological solutions to upgrade this compost into valuable products.

5 01 The BIOrescue project OBJECTIVES To demonstrate an innovative and resource-efficient biorefinery concept for mushroom compost conversion; To create valuable bio-based products from mushroom compost and other lignocellulosic feedstocks; To achieve a 20% overall cost-reduction in the enzymatic hydrolysis process; To reduce disposal costs for mushroom compost and generate a new income stream for mushroom producers.

6 01 The BIOrescue Project 6

01 The BIOrescue project BIOPESTICIDES FROM MUSHROOM

production and bioencapsulation Validate the

Validate the biopesticide toxicity (DL50 and DL90)

Bioassays Fractionation & enzymatic hydrolysis

7 01 The BIOrescue project BIOPESTICIDES FROM MUSHROOM COMPOST Sugar fermentation for biopesticide production and bioencapsulation Validate the fermentability for this concrete application Validate the biopesticide toxicity (DL50 and DL90) for at least 4 different Lepidoptera's plagues Bioassays Fractionation & enzymatic hydrolysis Fementation Bioencapsulation Mushroom compost Sugars Biopesticide Encapsulated biopesticide

Testing of biopesticide loading and release by enzymatic degradation TC pretreatment Mushroom

8 01 The BIOrescue project BIOPESTICIDES FROM MUSHROOM COMPOST Biopesticide and bioencapsulation: Nannocapsules obtained using biodegradable soluble-lignin through inverse emulsion polymerization Testing of biopesticide loading and release by enzymatic degradation TC pretreatment Mushroom compost Water soluble lignin Cross-linked lignin nanocapsules Enzymatic cleavage & biopesticide release

Erlenmeyer up to Bioreactor 30 L: Verify

Microspcopy Dry matter Protein (crystals):

9 01 The BIOrescue project BIOPESTICIDES FROM MUSHROOM COMPOST Process upscale from Erlenmeyer up to Bioreactor 30 L: Verify the fermentation process through: Microspcopy Dry matter Protein (crystals): concentration size Validate biopesticide Bioassays Pilot-scale_ Bioreactor (30L) Special Issue "Bacillus thuringiensis Toxins" Labscale_ErlenMeyer

10 01 The Biorescue Project BIOrescue project CONTACT Project coordinator Inés Del Campo CENER National Renewable Energy Centre Communication team Bénédicte Julliard Greenovate! Europe 10

11 01 The BIOrescue project The BIOrescue project has received funding from the Bio Based Industries Joint Undertaking under the European Union s Horizon 2020 research and innovation programme under grant agreement No

12 2. CENER Biomass Department Capabilities

13 Mission and Vision CENER Introduction Vision To be a research centre of excellence in the renewable energies field with international outreach. Mission To generate knowledge in the renewable energy field and to transfer it to the industry in order to boost sustainable energy development. Areas Wind Biomass Solar Photovoltaic Solar Thermal Energy Grid Integration Energy in Building 13

Science Technology Enterprise System CENER Introduction Basic Research Applied Research Technological Development Technological Services

14 Science Technology Enterprise System CENER Introduction Basic Research Applied Research Technological Development Technological Services Engineering Industrial Production Activities Applied Research & Technology Transfer. Assessment, Approval, Accreditation and Certification Services. 14

CENER in numbers CENER Introduction 21,2 M 200 100 M The annual 2013 budget is 21,2 million. 60% self-financing. 200 employees.

15 CENER in numbers CENER Introduction 21,2 M M The annual 2013 budget is 21,2 million. 60% self-financing. 200 employees. researchers, technical staff and support personnel. Total investments ( ) > 100 million. More than 200 customers in all five continents. 15

16 Infrastructures CENER Introduction Headquarters Sarriguren Wind Test Laboratory Sangüesa Advanced Biofuels & Bio-based products Plant Aoiz Experimental Wind Farm Alaiz Microgrid Sangüesa Offices: Sevilla México 16

Biomass Department Capabilities Areas Analytical Services Solid Biofuels Liquid Biofuels

Municipal Solid Wastes) Biochemical Processes Development Pretreatment and Conditioning of

Techno-Economic and Feasibility Studies.

and Pelletization). Thermochemical Processes (Gasification).

17 Biomass Department Capabilities Areas Analytical Services Solid Biofuels Liquid Biofuels Alternative Biomasses (e.g. Municipal Solid Wastes) Biochemical Processes Development Pretreatment and Conditioning of Materials. Biological Conversion Processes (E.g. Enzymatic Hydrolysis; Fermentation). Techno-Economic and Feasibility Studies. Thermochemical Process Development Pretreatment and Conditioning of Materials (Torrefaction and Pelletization). Thermochemical Processes (Gasification). Techno-Economic and Feasibility Studies. Sustainable Management of Biomass Quantification of Biomass Resources per Area. Logistic of Materials. Energy Analysis and Environmental Impacts (LCA) Development of Sustainability Management Systems. 17

18 Biomass Department Capabilities Experimental Facilities Headquarter Sarriguren Laboratories Sarriguren Second Generation Biofuel Center (CB2G) Aoiz R&D Bench Pilot Demo Lab Market 18

Biochemical Processes Development Biological Conversion Processes Carbohydrates Analytical Services Starch, pectin, inulin, uronic acids Cellulose, hemicellulose and lignin: NREL or van Soest

19 Biochemical Processes Development Biological Conversion Processes Carbohydrates Analytical Services Starch, pectin, inulin, uronic acids Cellulose, hemicellulose and lignin: NREL or van Soest Procedures Ash Protein Bradford, SDS page, CHN elemental analysis Calorific value Sugars Monomers: glucose*, xylose-fructose-galactose*, arabinose*, ribose*, fucose*; dimmers: cellobiose*, maltose*; trimmers: maltotriose* Organic acids Citric*, galacturonic*, succinic*, lactic*, acetic*, levulinic*, formic, fumaric* Alcohols: HPLC: glycerol*, sorbitol*;gc-fid: ethanol, butanol, methanol Inhibitors Furfural, 5-HMF, Benzaldehyde, ferulic acid, vainillin-syringaldehyde, vainillinic/syringic, coumaric, benzoic, 4-hydroxibenzoic, phenol, syiringol, guayacol 19

5bar & 200ºC Stirred tank reactor (75 l) able

20 Biochemical Processes Development Biological Conversion Processes Resources & Facilities Pilot Plant Unit Continuous horizontal reactor: 5Kg/h up to 14.5bar & 200ºC Stirred tank reactor (75 l) able to develop high consistency EH Bioreactors fully monitored (40 & 100 L) 20

21 Biochemical Processes Development Biological Conversion Processes Resources & Facilities Biochemical Semi-Industrial Plant Enzymatic hydrolysis (2 x 3 m3) Bioreactors (1, 3 and 6 m3) 21

22 Departamento de Biomasa

23 Departamento de Biomasa

24 Departamento de Biomasa

25 More information: Inés del Campo. Senior R&D Engineer 25

ACKNOWLEDGEMENTS The construction of the CB2G

Scientific- Technological Infrastructures) and

26 ACKNOWLEDGEMENTS The construction of the CB2G has been funded by the Government of Navarre (Navarre Plan 2012), the Spanish Ministry of Science and Innovation (National Program for Scientific- Technological Infrastructures) and the European Fund for Regional Development (FEDER)