University of Guelph. Supply Center Product List

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1 University of Guelph Supply Center Product List

2 Supply Center Location Science Complex Building 140 To find out more about your Supply Center, request product additions to the Supply Center, or find out more about Thermo Fisher Scientific products and services, contact: Allison MacMullin BioSciences Account Manager Mobile (905) Need a product added to your Supply Center? Please contact: SupplyCenterProductRequests@thermofisher.com Technical Support Hours of operation: 9:00 a.m. to 8:00 p.m. ET Phone: techsupport@thermofisher.com Supply Center Customer Service Phone: , option 5, option 1, ext supplycenters@thermofisher.com For Research Use Only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.

3 Blasticidin S HCl, powder 50 mg R Zeocin Selection Reagent 8 x 1.25 ml R Hygromycin B (50 mg/ml) 20 ml Penicillin-Streptomycin (10,000 U/mL) Antibiotic-Antimycotic (100X) ml Trypsin-EDTA (0.5%), no phenol red Trypsin-EDTA (0.25%), phenol red Trypsin-EDTA (0.05%), phenol red ml Fetal Bovine Serum, qualified, USDA-approved regions Fetal Bovine Serum, qualified, Canada origin Lipofectamine 2000 Transfection Reagent 1.5 ml Lipofectin Transfection Reagent 1 ml Lipofectamine 3000 Transfection Reagent 1.5 ml L CloneJET PCR Cloning Kit 20 reactions K CloneJET PCR Cloning Kit 40 reactions K sheets High-Capacity cdna Reverse Transcription Kit SuperScript VILO cdna Synthesis Kit SuperScript First-Strand Synthesis System for RT-PCR SuperScript III First-Strand Synthesis System Cell Culture & Transfection Reagents Antibiotics & Antimycotics Cell Culture Supplements B-27 Supplement (50X), serum free Cell Dissociation Reagents Cryopreservation Media Recovery Cell Culture Freezing Medium Serum Transfection Reagents DNA Vectors, Clones, Purified Nucleic Acids & Libraries DNA Vectors Instruments and Equipment Gel Boxes, Gel Imagers, Power Supplies & Accessories CL-XPosure Film, 8 x 10 in. (20 x 25 cm) Kits and Assays cdna Synthesis, Library, & Cloning Kits

4 GeneJET Plasmid Miniprep Kit 2 K GeneJET Gel Extraction Kit K GeneJET PCR Purification Kit K PureLink HiPure Plasmid Midiprep Kit 25 preps K PureLink Quick Plasmid Miniprep Kit K PureLink Quick Plasmid Miniprep Kit 2 K PureLink Quick Gel Extraction Kit K PureLink PCR Purification Kit K TRIzol Reagent 200 ml TRIzol Reagent PureLink RNA Mini Kit A PowerUp SYBR Green Master Mix 5 ml A Power SYBR Green PCR Master Mix 1 x 5 ml Fast SYBR Green Master Mix 1 x 5 ml Platinum SYBR Green qpcr SuperMix-UDG Platinum SYBR Green qpcr SuperMix-UDG 500 reactions g x 1 ml RNAlater Stabilization Solution AM RNaseZap RNase Decontamination Solution 250 ml AM DNAZap PCR DNA Degradation Solutions 250 ml AM DNA Gel Loading Dye (6X) 5 x 1.0 ml R BlueJuice Gel Loading Buffer (10X) 3 x 1 ml UltraPure Agarose 100 g UltraPure Agarose 500 g UltraPure Phenol 500 g IPTG 1g DNA RNA Purification Kits & Reagents Real-Time PCR and Endpoint RT-PCR Kits Lab Reagents and Chemicals Denaturing Agents UltraPure Formamide DNA RNA Reagents UltraPure Salmon Sperm DNA Solution DNA RNA Storage & Nuclease Control Reagents Electrophoresis Reagents & Buffers General Chemicals for Life Science Molecular Biology Enzyme Buffers PCRX Enhancer System Water UltraPure DNase/RNase-Free Distilled Water

5 units R Pierce ECL Western Blotting Substrate Step NBT/BCIP Substrate Solution 250 ml X-Gal 100 mg SimplyBlue SafeStain 1L LC SYBR Safe DNA Gel Stain 400 µl S Oligo(dT)18 Primer 60 µl SO Oligo(dT)12-18 Primer 25 µg Random Primers 9 units dntp Set 100 mm Solutions 4 x 0.25 ml R dntp Mix (10 mm each) 1 ml R dntp Set (100 mm) 4 x 250 µl dntp Mix (10 mm ea) 100 µl RiboLock RNase Inhibitor (40 U/µL) 2, EO RNaseOUT Recombinant Ribonuclease Inhibitor 5,000 units Ribonuclease Inhibitor, Cloned 1,000 units Phusion High-Fidelity DNA Polymerase (2 U/µL) F530S Phusion Hot Start II DNA Polymerase (2 U/µL) F549L Phusion Hot Start II DNA Polymerase (2 U/µL) F549S Rapid DNA Ligation Kit K FastAP Thermosensitive Alkaline Phosphatase (1 U/µL) 1,000 units EF T4 Polynucleotide Kinase (10 U/µL) EK T4 DNA Ligase (5 U/µL) 1,000 units EL T4 DNA Ligase (5 U/µL) 200 units EL RNase H (5 U/µL) EN DNase I, RNase-free (1 U/µL) 1,000 units EN RNase A, DNase and protease-free (10 mg/ml) 10 mg EN Proteinase K, recombinant, PCR grade 1 ml EO Klenow Fragment (10 U/µL) 300 units EP Labeling & Detection Products Cell Stains Trypan Blue Solution, 0.4% Cellular Structure Probes Rhodamine Phalloidin Enzyme Substrates Gel & Membrane Stains Oligos, Primers, Probes & Nucleotides DNA Primers & Oligos Nucleotides Proteins, Enzymes, & Peptides Enzyme Inhibitors PCR & Cloning Enzymes * Prices subject to change

6 T7 RNA Polymerase (20 U/µL) 5,000 units EP Taq DNA Polymerase, recombinant (5 U/µL) EP RevertAid H Minus Reverse Transcriptase (200 U/µL) 10,000 units EP DreamTaq Hot Start DNA Polymerase 200 U EP Kpn2I (BspEI) (10 U/µL) ER FastDigest BamHI 800 reactions FD FastDigest BglII FD FastDigest EcoRI 800 reactions FD FastDigest Eco32I 400 reactions FD FastDigest HindIII 800 reactions FD FastDigest KpnI FD FastDigest NcoI FD FastDigest NdeI FD FastDigest NotI FD FastDigest PstI 800 reactions FD FastDigest SalI FD FastDigest SmaI FD FastDigest XbaI FD FastDigest XhoI 400 reactions FD FastDigest BclI FD FastDigest NheI FD FastDigest SacI FD FastDigest ApaI FD FastDigest DpnI FD Platinum Taq DNA Polymerase 120 reactions Platinum Taq DNA Polymerase 600 reactions Platinum Taq DNA Polymerase High Fidelity Platinum Taq Green Hot Start DNA Polymerase 120 reactions Platinum Hot Start PCR Master Mix (2X) T4 DNA Ligase (1 U/µL) DNA Polymerase I, Large (Klenow) Fragment Ribonuclease H 30 units Taq DNA Polymerase, native SuperScript II Reverse Transcriptase 10,000 units DNase I, Amplification Grade SuperScript III Reverse Transcriptase 10,000 units Proteinase K (Fungal) 100 mg Proteinase K (Fungal) 1g M-MLV Reverse Transcriptase (200 U/µL) 40,000 units Anza 10 DpnI 800 units IVGN AmpliTaq Gold DNA Polymerase with Buffer II and MgCl2 250 units N Lambda DNA/HindIII Marker, ready-to-use 5 x 50 µg SM GeneRuler 100 bp DNA Ladder 50 µg SM GeneRuler 1 kb DNA Ladder 5 x 50 µg SM GeneRuler 1 kb DNA Ladder, ready-to-use 5 x 50 µg SM GeneRuler 100 bp Plus DNA Ladder 50 µg SM GeneRuler 1 kb Plus DNA Ladder 5 x 50 µg SM Low DNA Mass Ladder 23.5 µg TrackIt 1 Kb Plus DNA Ladder 50 µg Kb Plus DNA Ladder 250 µg Kb Plus DNA Ladder 1000 µg bp DNA Ladder 50 µg bp DNA Ladder 250 µg Standards, Ladders & Controls DNA RNA Ladders

7 PageRuler Prestained Protein Ladder, 10 to 180 kda 2 x 250 µl MagicMark XP Western Protein Standard 250 µl LC Novex Sharp Pre-stained Protein Standard 2 x 250 µl LC SeeBlue Plus2 Pre-stained Protein Standard 500 µl LC BenchMark Protein Ladder 2 x 250 µl BenchMark Pre-stained Protein Ladder 2 x 250 µl Protein Standards & Ladders

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