Rent a Bioreactor. Bioreactor & Fermentation Technology at acib

Transcription

1 Rent a Bioreactor Bioreactor & Fermentation Technology at acib

2 Foto: acib

3 Content Microbial Fermentation 05 Animal Cell Fermentation 05 Cell Harvest 07 Filtration 08 Separation 08 Analytics 10 Our Services 11 About acib & Contact 12

4 Rent a Bioreactor The Austrian Centre of Industrial Biotechnology (acib) has been supporting companies and scientific partners for decades. We understand how important the right know-how and facilities can be to successfully conclude projects. We also know, how expensive it can be to install and maintain the latest state-of-the-art equipment, especially in case of huge bioreactors. The new solution at acib Making available the expertise and facilities you need for your fermentation and/or bioprocess development. Our experts provide you with offers tailored for your specific project and you choose whether to: k rent-a-bioreactor / DSP for your project k train your staff k let us do all the work for you A detailed list of available equipment for your projects is on the next pages. 3

5 RENT A BIOREACTOR Foto: acib 4 Foto: acib

6 Upstream Processing MICROBIAL FERMENTATION Fermentation for bacteria, yeast and fungi k Fully automated process control (Allen Bradley/Rockwell) k Supply with steam, ice water, cooling water and compressed air k 2 modular bioreactors (10 20 L, L) k 30 L and 200 L (working volume) stirred tank reactor k Storage/fermentation vessels (1250 L, 2000 L, 3600 L) with double jacket, stirrer and aeration k Additional mobile vessels for storage and media preparation available Labscale fermentation for bacteria, yeasts and fungi k Incubation in shake flasks k DASGIP parallel bioreactor system ( 4 1 L bioreactors) k Infors Multifors II (4 2 L bioreactors) with offgas analysis (BlueSense) k Sartorius Biostat Bplus (2 L bioreactor) k Biostat Ed. (2 5 L bioreactors), modified and adaptable k Additional access to high throughput micro bioreactor systems (BioLector ), lab scale bioreactors equipped with online monitoring systems ANIMAL CELL FERMENTATION Fermentation of CHO, hybridoma and insect cells k including automated process control k 70 L scale stirred tank bioreactor (fully automated) k 500 L airlift bioreactor k 1600 L scale stirred tank bioreactor k Additional access to small and lab scale bioreactors/systems (shake flask, DASGIP, WAVE ) 5

7 RENT A BIOREACTOR 6 Foto: acib

8 Downstream Processing CELL HARVEST Centrifugation k Several labscale centrifuges k High performance centrifuge with continuous feed and discountinuous discharge (Contifuge) with 22,000 g and solid volume 0.3 L k Disc-stack centrifuge (GEA PSC-6) with 8000 m 2 equivalent clarification area; L/h throughput and other Westfalia Disc Separator units High Pressure homogenization (HPH) k Two-stage high pressure homogenizer 1500 bar k Benchtop system for lab scale and optimization (10 L/h throughput) k Pilot scale system variable ( L/h throughput) 7 Foto: acib

9 RENT A BIOREACTOR FILTRATION Dead-end Filtration k Filter test system for filter disks (14 cm 2 ) and capsules filtration operation up to 100 L/batch with pressurized closed vessels Ultra-/Diafiltration k Small scale ( cm 2 ) and k Pilot scale systems (up to 2 m 2 membrane area) with automated recording of all system data (pressure, fluxes, etc.) k Systems can be operated in different control modes (constant flux, constant trans-membrane pressure, min and max inlet pressure) k Sterile filtration for intermediates and final products Micro- and ultrafiltration (crossflow ceramic modules) k 2 microfiltration modules Atech (0.2 µm) with 0.13 m² filter area each k 2 microfiltration modules Atech (0.2 µm) with 0.2 m² filter area each k 2 microfiltration modules Atech (0.2 µm) with 0.58 m² filter area each k 2 ultrafiltration modules Atech (0.05 µm) with 0.2 m² filter area each k Pumps, casings, storage vessels Special filtration techniques k Vacuum drum filter VELO FRP 1 (1 m² filter area, 500 L/h) SEPARATION Precipitation and refolding k (Fed-)Batch operation with temperature control, headspace gas overlay and on-line monitoring probes. Operating volume L (suitable for precipitation and refolding reactions) Chromatography k ÄKTA explorer 10 and 100 for development and small scale preparative purification k ÄKTA Pilot for pilot scale chromatography up to 40 L/h, Column size 0,5 10 L k ÄKTA purifier and ÄKTA pure (labscale chromatography) 8

10 SEPARATION Lyophilization k Labonco Freezone 2.5 plus, labscale k Christ Gamma 1-16 LSC plus, lab-/pilot scale Fluid bed drying/granulation k AMMAG A-WT 43 multifunctional fluid bed drier for drying, granulation and coating, pilot scale (ca. 50 L batch size) Distillation/Rectification k plants of different sizes (DN 10 DN 150) k different operation modes (discontinuous, continuous) k adjustable for different separation problems at ambient pressure and vacuum operation k thin film evaporator k rotary evaporators (up to 20 litres) k steam distillation Liquid Liquid Extraction plants k mixer-settler plants of different sizes (ml up to several litres) k extraction columns with different internals (packed, sieve tray, RDC, TCDC and operation mode (agitated, pulsed) CO 2 -technology k extraction plant (300 ml, 690 bar, 240 C up to 10 litres, 325 bar, 200 C, including stirrer) k impregnation plant k high pressure view cell (400 bar, 100 C) k micro-mixer fractionation plant for liquid feed material k extraction column (2 m active height, 35,4 mm inner diameter, 180 bar, 100 C, settler with view glasses at bottom and top) Drying k spray dryer (MINOR plant, d = 800 mm, h = 600 mm, 80 kg/h air, max. inlet temperature = 340 C) k fluidized bed dryer 9

11 RENT A BIOREACTOR Membrane unit OSMO k flat sheet membranes for MF, NF, UF, RO (max. 64 bar) Single separation equipment k disc centrifuge (pilot plant size) Foto: acib ANALYTICS k Acid/base titration, Karl Fischer k GC-FID, GC-MS, GC-ECD k HPLC k Ion chromatography k AAS k TOC k Drying balance k PAAR densiometer k PAAR Stabinger viscosimeter k surface tension (ring, droplet) 10

12 Our Services We provide tailored calculations for each project! In case of sensitive data, we can work under protection of a CDA/nDA. Aside from contract research, we are also looking forward to supporting you in national or international funding projects. EXAMPLES k Process development for up- and downstream processing for bacteria, yeasts, fungi and higher eukaryotic systems k Fermentation scale up from µ- to lab- and pilot scale k On-line monitoring platform and development of monitoring strategies for critical points in real-time in up- and downstream k Manufacturing and processing of products at pilot scale (non-gmp, but GMP-like conditions available) k Available in-house model systems e.g.: rhsod, GFP, rh-antitnfα, rhtnfα mab, scfv (IB or soluble) k Available model organisms: E. coli, P. pastoris, CHO, Sf9-insect cells, etc. also open for model systems and organisms provided by partner k Method development for cell harvest, IB harvest, refolding, precipitation, small scale development and transfer to pilot scale k Filter testing for depth- and sterile filtration including upscale k Ultra-/Diafiltration incl. method development and upscale; membrane testing k Chromatographic separation; method development and upscale; bench marking of different resins with in-house model systems (different feed) k Test of equipment provided by supplier k Individual training for single or multi-unit operations including academic level education in bioprocess engineering k complete process development from determination of thermodynamic data, lab scale experiments and to pilot scale experiments k experimental validation of the downstream process k modelling, simulation and feasibility studies of single separation units up to complete processes k economic and environmental process evaluation 11

13 RENT A BIOREACTOR The Austrian Centre of Industrial Biotechnology ABOUT ACIB The Austrian Centre of Industrial Biotechnology (acib) is a top-class international research institution in the field of industrial biotechnology. Since its foundation in 2010 the centre of has specialized on the development of innovative, eco-friendly and economical processes for the biotech-, chemical- and pharmaceutical industries using the methods and tools of nature. Presently 200+ coworkers are carrying out research in more than 175 research projects. CONTACT FOLLOW US ON acib GmbH Dr. Martin Trinker Director Business Development & Fundraising Petersgasse 14 A-8010 Graz martin.trinker@acib.at INNOVATIONS FROM NATURE

14 INNOVATIONS FROM NATURE