Product Datasheet. AIF-1/Iba1 Antibody NB SS. Unit Size: 0.02 mg. Store at -20C. Avoid freeze-thaw cycles. Reviews: 12 Publications: 70

Transcription

1 Product Datasheet AIF-1/Iba1 Antibody NB SS Unit Size: 0.02 mg Store at -20C. Avoid freeze-thaw cycles. Reviews: 12 Publications: 70 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/18/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB SS AIF-1/Iba1 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer Target Molecular Weight 0.02 mg 0.5 mg/ml Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.02% Sodium Azide IgG Immunogen affinity purified Tris (ph 7.3), 0.5% BSA 17 kda Page 1 of 5 v.20.1 Updated 2/18/2019 Product Description Host Goat Gene ID 199 Gene Symbol Species Reactivity Notes Marker AIF1 Human, Mouse, Rat, Porcine, Canine Mouse reactivity reported in scientific literature (PMID: ). Canine reactivity per customer review. pan-microglia Marker Specificity/Sensitivity This antibody is expected to recognize isoform 1 (NP_ ) and isoform 3 (NP_ ). AIF1 / IBA1 is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the antiinflammatory response to vessel wall trauma. Immunogen Peptide with sequence C-TGPPAKKAISELP, from the C Terminus of the protein sequence according to NP_ ; NP_ Product Application Details Applications Recommended Dilutions Application Notes Western Blot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry- Paraffin, Peptide ELISA, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount Western Blot 1-3 ug/ml, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Peptide ELISA Detection Limit 1:128000, Immunohistochemistry Free-Floating, Immunohistochemistry Whole-Mount WB: ~17 kda band observed in Human Brain (Frontal Cortex) and Rat Brain lysates (calculated MW of 16.7kDa according to Human NP_ and 16.8kDa according to Rat NP_ ). IHC: Use reported in scientific literature and customer reviews. However, variation betwen lots has been observed. ICC: Use reported in scientific literature (PMID: ). Use in Immunohistochemistry, Western blot, Immunohistochemistry frozen, Immunohistochemistry-free floating, Immunohistochemistry-paraffin, and Immunocytochemistry/immunofluorescence reported in multiple pieces of scientific literature. Use in Immunohistochemistry-Whole mount reported in scientific literature (PMID: ). Please contact technical support if you have any questions.

3 Images Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB ] - Immunoreacitiy in a mouse spinal cord after focal injection of lysolecitithin into the ventral column white matter. Image supplied by a certified customer review. Page 2 of 5 v.20.1 Updated 2/18/2019 Immunohistochemistry Free-Floating: AIF-1/Iba1 Antibody [NB ] - Staining in mouse brain. Image supplied by a certified customer review. Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB ] - Mouse spinal cord microglia staining in frozen sections. Image supplied by a certified customer review. Immunohistochemistry-Frozen: AIF-1/Iba1 Antibody [NB ] - Staining in rat hippocampus at 40x. Image supplied by a certified customer review.

4 Immunohistochemistry-Paraffin: AIF-1/Iba1 Antibody [NB ] - Staining in mouse brain. Image supplied by a certified customer review. Page 3 of 5 v.20.1 Updated 2/18/2019 Immunohistochemistry: AIF-1/Iba1 Antibody [NB ] - Staining in Mouse brain. Image supplied by a certified customer review. Immunohistochemistry: AIF-1/Iba1 Antibody [NB ] - Staining in canine optic nerve. Western Blot: AIF-1/Iba1 Antibody [NB ] - Staining of human frontal cortex (A), Rat Brain (B), Mouse Brain (C), and Pig Brain (D) lysates (35 ug protein in RIPA buffer). Antibody at 1 ug/ml. Detected by chemiluminescence.

5 Publications Polis B, Srikanth KD, Elliott E et al. L-Norvaline Reverses Cognitive Decline and Synaptic Loss in a Murine Model of Alzheimer's Disease Neurotherapeutics Oct :00AM [PMID: ] (IHC-P, Mouse) Abraham D, Feher J, Scuderi GL et al. Exercise and probiotics attenuate the development of Alzheimer's disease in transgenic mice: Role of microbiome Exp. Gerontol. Dec :00AM [PMID: ] (IHC, Mouse) Sprenkle NT, Lahiri A, Simpkins JW, Meares GP. Endoplasmic reticulum stress is transmissible in vitro between cells of the central nervous system J. Neurochem. Dec :00AM [PMID: ] (WB, Mouse) Rivera PD, Hanamsagar R, Kan MJ et al. Removal of microglial-specific MyD88 signaling alters dentate gyrus doublecortin and enhances opioid addiction-like behaviors. Brain Behav. Immun. Nov :00AM [PMID: ] (IHC, Mouse) Galiazzo G, Giancola F, Stanzani A et al. Localization of cannabinoid receptors CB1, CB2, GPR55, and PPARalpha in the canine gastrointestinal tract. Histochem Cell Biol 2018 Jun 7 [PMID: ] Wohleb ES, Terwilliger R, Duman CH, Duman RS. Stress-Induced Neuronal Colony Stimulating Factor 1 Provokes Microglia-Mediated Neuronal Remodeling and Depressive-like Behavior. Biol Psychiatry 2018 Jan 1 [PMID: ] Mao SPH, Park M, Cabrera RM et al. Loss of amphiregulin reduces myoepithelial cell coverage of mammary ducts and alters breast tumor growth. Breast Cancer Res. Oct :00AM [PMID: ] (ICC/IF, Mouse) Zhang C, Lai MB, Pedler MG et al. Endothelial Cell-Specific Inactivation of TSPAN12 (Tetraspanin 12) Reveals Pathological Consequences of Barrier Defects in an Otherwise Intact Vasculature. Arterioscler. Thromb. Vasc. Biol. Nov :00AM [PMID: ] (ICC/IF, Mouse) Lenz KM, Pickett LA, Wright CL et al. Mast Cells in the Developing Brain Determine Adult Sexual Behavior. biorxiv. [PMID: ] (IHC, Rat) Bussian TJ, Aziz A, Meyer CF et al. Clearance of senescent glial cells prevents tau-dependent pathology and cognitive decline. Nature Sep :00AM [PMID: ] (IHC, ICC/IF, Mouse) Xu H, Mu S, Qin W. Microglia TREM2 is required for electroacupuncture to attenuate neuroinflammation in focal cerebral ischemia/reperfusion rats Biochem. Biophys. Res. Commun. Aug :00AM [PMID: ] (ICC/IF, Rat) Hou Y, Lautrup S, Cordonnier S et al. NAD+ supplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency Proc. Natl. Acad. Sci. U.S.A. Feb :00AM [PMID: ] (ICC/IF, Mouse) More publications at Page 4 of 5 v.20.1 Updated 2/18/2019

6 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: