CORE FACILITY USER FEES FEES FLOW CYTOMETRY CORE FACILITY

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1 CORE FACILITY USER FEES This document is for researchers to use for grant applications in In December 2018 fees will be increased by the CPI rate for WIMR facilities (current rate as at September 2017 is 1.8%). Please include this increase in your budget for costs related to your forecast budget in 2019, 2020 and 2021 budget. Fees have been calculated using a cost recovery method, and are intended to offset the cost of operating the facilities. This cost recovery model will ensure continued investment into these facilities, bringing researchers at Westmead world class technology to conduct their research. FEES FLOW CYTOMETRY CORE FACILITY The Flow Cytometry Core Facility is co-located at WIMR and CMRI. /SERVICES TYPE LOCATION Canto A Analyser - Simple CMRI FC500 Analyser - Simple CMRI CantoII Analyser - Intermediate WIMR Fortessa Analyser - Complex WIMR LSRII Analyser - Complex WIMR AriaIII Sorter - Simple WIMR Influx Sorter - Complex WIMR AutoMACS Cell Separator WIMR GentleMACS Tissue Dissociator WIMR Luminex 200 Cytokine Multiplex WIMR Cell Sorters - Intermediate - $75 per hour Cell Sorters - Complex - $75 per hour Cell Sorter Setup - $75 per order Analysers - Simple $20 per hour $70 per hour Analysers - Intermediate $30 per hour $80 per hour Analysers - Complex $40 per hour $90 per hour AutoMACS Pro $20 per hour $70 per hour GentleMACS $5 per hour $55 per hour Luminex 200 $5 per hour $55 per hour Assistance/Service - $50 per hour Training - Initial - $200 all instruments Training - Subsequent - No Charge FlowJo Site License $100/Quarter* Note: consumables are not included. * Price may change depending on USD to AUD exchange rate. ers can be trained on Analysers: Full training includes theory, instrument operation and data analysis. Subsequent trainings are for instruments only. Cell Sorting services are provided by facility staff. Suat Dervish Flow Cytometry Advanced Specialist T E suat.dervish@sydney.edu.au Edwin Lau Flow Cytometry Specialist Technician T E edwin.lau@sydney.edu.au Christine Smyth Children s Medical Institute Flow Cytometry Scientist T E csmyth@cmri.org.au WRH Core Facility Fees 2018 for ers Page 1 of 6

2 CELL IMAGING CORE FACILITY The Cell Imaging Core Facility is located at WIMR. Simple/Analysis $12 per hour s $30 per hour $80 per hour Advanced Microscopes $40 per hour $90 per hour Assistance - $50 per hour Transmission to EM Service - Quoted per service Training Intermediate Microscopes Training Advanced Microscopes Subscription 7% discount* $1,500 Subscription 20% discount* $2,500 Subscription 30% discount* $5,000 Subscription 47% discount* $8,000 - $100 per instrument - $250 per instrument *discount of hourly rate until subscription amount is used up Dr Hong Yu Cell Imaging Advanced Specialist T E hong.yu@sydney.edu.au Dr Laurence Cantrill Kid s Institute Core Facility Manager T E laurence.cantrill@health.nsw.gov.au CLEM (Leica SP5 Confocal) Olympus Confocal Deltavision Zeiss Live Cell Imaging Laser Capture Microdissection Nuance Multispectral Imaging System Olympus Bx53/DP80 Fluorescence microscope TYPE Advanced Microscope Advanced Microscope Advanced Microscope ELECTRON MICROSCOPY CORE FACILITY The Electron Microscopy Core Facility is located in the ICPMR building of the Westmead Hospital. Electron Microscope $40 per hour $90 per hour Training Electron Microscope (Basic) - $250 per instrument Assistance/Service/ Training - Other - $50 per hour Ultramicrotome $20 per hour $70 per hour Cryo-ultramicrotome $30 per hour $80 per hour Carbon Coater $10 per hour $60 per hour TYPE FEI Tecnai 20 TWIN200kV TEM Transmission Electron Microscope FEI CM120 BioTWIN120kV TEM Transmission Electron Microscope Leica UC7 / FC7 Cryo-Ultramicrotome Emma Kettle Electron Microscopy () Advanced Specialist T E ekettle@cmri.org.au Dongwei (Lucy) Wang Electron Microscopy () Specialist Technician T E dongwei.wang@sydney.edu.au Leica UC6 Leica UCT Edwards 306 Carbon Coater Ultramicrotome Ultramicrotome Carbon Coater WRH Core Facility Fees 2018 for ers Page 2 of 6

3 PROCESS / TECHNIQUE CONSUMABLES ONLY SERVICE CHARGE Manual process aldehyde fixed cells / tissues(max 6 samples / six blocks per sample) to epoxy resin $21 +$100 Auto process (up to 16 samples / 6 blocks per sample) aldehyde fixed cells / tissues to epoxy resin $50 +$50 Manual process aldehyde fixed cells / tissue (max 6 samples / six blocks per sample) to acrylic resin $ $100 Ultramicrotomy of resin embedded tissue /hr $20 +$50 Fix and process one (1) sample / 4 blocks aldehyde fixed cells / tissue for Tokuyasu technique $15 +$75 Cryo-ultramicrotomy (Tokuyasu technique) 1 block, up to 12 grids n/a $150 Supply sapphire discs 1.4mm for cell monolayer culture / HPF -each $6.50 n/a Supply sapphire discs 3mm for cell monolayer culture / HPF -each $5.30 n/a Supply sapphire discs 3mm gridded for cell monolayer culture / HPF -each $20 n/a Supply sapphire discs 6mm for cell monolayer culture / HPF -each $11.20 n/a Supply MatTek gridded dishes $8.50 n/a Supply specialist micro-biopsy needle / 5 carriers for HPF of tissue $35 n/a Cryo-fixation of cell monolayers on sapphire discs by HPF (max 10 discs) $50 +$100 Process cell monolayers on sapphire discs by FS / LT embedding for immunolabelling (max 10 discs) $120 +$100 Process cell monolayers on Thermanox by modified FS / LT embedding for immunolabelling (max 16) $80 n/a Process cell monolayers on sapphire discs from HPF using FS / LT embedding + TAMOI for n/a $450 morphology / tomography Preparation of thin film samples for cryo-tem using plunge freezing 4 grids n/a $200 On-section immunogold labelling using Leica IGL (researcher supplies slides, 1 o & 2 o antibodies) $38 +$75 Fix and process cells/tissue blocks for Scanning Electron Microscopy $15 +$50 Fix and process cells/tissue blocks for Serial Block Face SEM $60 +$200 Negative Stain $5 +$25 Grid film coating (formvar, pioloform) $5 +$25 Cost estimate only, dependent upon substrates employed Note: These costs are for consumables and (assisted) processing only and do not include microscope time. Fixed fee for Electron Microscopy training includes training to a proficient level of basic techniques. Supplementary training or training on advanced techniques is charged per hour. Training on other instruments is also charged per hour. Prices for advanced preparatory techniques/services will be provided at a later date. WRH Core Facility Fees 2018 for ers Page 3 of 6

4 GENOMICS CORE FACILITY The Genomics Core Facility is located at WIMR. Genomics equipment $25 per hour $75 per hour Training equipment - $50 per hour Assistance/Service - $50 per hour Bioanalyzer 2100 Tapestation 4200 Nanostring ncounter PrepStation Nanostring ncounter Digital Analyzer Digital PCR Droplet Generator QX200 Digital PCR Droplet Reader C1000 Thermocycler Covaris Evolution TYPE Microfluidic electrophoresis system Microfluidic electrophoresis system Robotic liquid handling High resolution microscope Digital PCR Droplet Generator Digital PCR Droplet Reader Thermocycler Ultrasonicator Joey Lai Genomics Advanced Specialist T E joey.lai@sydney.edu.au PROCESS / TECHNIQUE CONSUMABLES SERVICE CHARGE Bioanalyzer (Small RNA Chip 11 samples per chip) - $95 per chip Tapestation Standard DNA/RNA Tape $4.50 per sample $6.10 per sample Tapestation High Sensitivity DNA/RNA Tape $6.00 per sample $7.60 per sample STR Genotyping Analysis (with DNA extraction) $33 per sample $90 per run * STR Genotyping Analysis (without DNA extraction) $24 per sample $90 per run * Next Gen Sequencing Library Preparation Nanostring Gene Expression (Standard scan) Nanostring Gene Expression (High sensitivity scan) Nanostring mirna Expression (Standard scan) Nanostring mirna Expression (High sensitivity scan) * NOT including consumables $7.20 per sample for DNA samples $29.90 per sample for RNA samples $500 per run for up to 96 samples * - $200 per cartridge * - $250 per cartridge * - $325 per cartridge * - $450 per cartridge * WRH Core Facility Fees 2018 for ers Page 4 of 6

5 BIOMEDICAL PROTEOMICS CORE FACILITY The Biomedical Proteomics Core Facility is located at CMRI. The Akta Pure equipment listed below is located at WIMR. Pricing information is currently unavailable for the CMRI facility. Please contact Core Facility Manager Val Valova for more details. Access to this facility is currently limited to trained users of the Westmead Hub. AKTA Pure $5 per hour $20 per hour AKTA Training $50 per person *Training provided at the discretion of the Core Facility Manager contact us for further details. Access includes use of wet lab. Charges based on actual instrument time usage. Val Valova Children s Medical Institute Core Facility Manager T E vvalova@cmri.org.au Eve Diefenbach Proteomics Advanced Specialist T WRH Core Facility Fees 2018 for ers Page 5 of 6

6 INCORPORATING THESE FEES INTO YOUR GRANT APPLICATIONS ESTIMATING COSTS FOR BUDGET REQUESTS The NHMRC requires detailed costings in their budgets; for example, numbers of samples, estimated analysis hours and justification of why the characterisation techniques are required for the project. To help plan your budget request, using the fees recommended in this document, we provide the following example. Example: The project requires access to an advanced microscope for one sample per week, at 4 hours per sample, for 45 weeks in Year 1. 1 x 4 x 45 = therefore 180hrs of beam time is required. Advanced microscope fees are $40 per hour if operated by a user 180 x 40 = therefore for Year 1, a total cost of $7,200 will need to be included. N.B. For estimates on the average number of samples per hour, please consult the appropriate Core Facility Manager. JUSTIFYING BUDGET REQUESTS The following wording can be used in your application to justify the funding request: This research project requires the examination of N samples per week/month/year [as appropriate] with the advanced microscopy and/or microanalysis [as appropriate] technique/s of [specify; e.g. atom probe tomography]. The estimated time required for characterisation of each sample is X hours, at a cost of $40 per hour of instrument time. You should also add further specific explanation of why the chosen microscopy technique/s is/are necessary for the research. WRH Core Facility Fees 2018 for ers Page 6 of 6