Product Datasheet. GFP Antibody NB Unit Size: 0.05 ml. Store at -20C. Avoid freeze-thaw cycles. Reviews: 3 Publications: 46

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1 Product Datasheet GFP Antibody NB Unit Size: 0.05 ml Store at -20C. Avoid freeze-thaw cycles. Reviews: 3 Publications: 46 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 10/10/2018 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB GFP Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity 0.05 ml 6.05 mg/ml Store at -20C. Avoid freeze-thaw cycles. Polyclonal 0.01% Sodium Azide IgG Immunogen affinity purified Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, ph 7.2 Target Molecular Weight 27 kda Page 1 of 6 v.20.1 Updated 10/10/2018 Product Description Host Gene Symbol Species Reactivity Notes Specificity/Sensitivity Immunogen Product Application Details Applications Recommended Dilutions Application Notes Rabbit GFP Jellyfish GFP recombinant expression in mouse reported in scientific literature (PMID: ). Reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP and EGFP. Human reactivity reported in scientific literature (PMID: ). Mouse reactivity reported in scientific literature (PMID: ). GFP from Aequorea victoria The immunogen is a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria. Western Blot, Chromatin Immunoprecipitation, ELISA, Electron Microscopy, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation Western Blot 1:1000-1:3000, Chromatin Immunoprecipitation, Flow Cytometry 1:10-1:1000, ELISA 1: :40000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500, Immunohistochemistry-Frozen 1:10-1:500, Electron Microscopy Immunoprecipitation reported successful by customer review. Immunohistochemistry-Paraffin (PMID: ) Immunocytochemistry/Immunofluorescence was reported in scientific literature.use in Flow Cytometry reported in scientific literature (PMID: ). Use in Electron Microscopy reported in scientific literature (PMID ). Use in Immunohistochemistry-Frozen reported in multiple pieces of scientific literature. Use in chromatin immunoprecipitation reported in scientific literature (PMID ).

3 Images Immunocytochemistry/Immunofluorescence: GFP Antibody [NB ] - Analysis of FITC conjugate of NB Staining of mouse spleen cells Tissue: spleen cells infected with MHV68-H2bYFP. As seen in: Collins CM, Speck SH (2012) Tracking Murine Gammaherpesvirus 68 Infection of Germinal Center B Cells. Page 2 of 6 v.20.1 Updated 10/10/2018 Western Blot: GFP Antibody [NB ] - WB analysis of GFP in various lysates. Image courtesy of product review by Emilie Leclerc. Western Blot: GFP Antibody [NB ] - Analysis of GFP HRP expression in HeLa/GFP cell lysate. Western Blot: GFP Antibody [NB ] - Lane 1: Wild type GFP (0.1 ug ) was used to spike HeLa whole cell lysate. Lane 2: none. Load: 30 ug per lane. Primary antibody: GFP antibody at 1:1000 for overnight at 4C. Secondary antibody: IRDye800 (TM) Goat-a-Rabbit IgG [H&L] MX10 at 1:10,000 for 45 min at RT. Block: 5% BLOTTO in PBS overnight at 4C. Predicted/Observed size: 27 kda for epitope tug GFP.

4 Western Blot: GFP Antibody [NB ] - Analysis using Biotin conjugate of NB Lane 1: 50ng of GFP. Lane 2: none. Primary antibody: none. Secondary antibody: Anti- Biotin Conjugated secondary antibody was used at 1:5000 in Blocking Buffer for Fluorescent Western Blotting for 45 min at RT. HRP Streptavidin was used at 1:40,000 in incubated with blocking buffer for 30 min at 20C. Block: 5% Blotto 30 min at 20C. Predicted/Observed size: 28 kda for GFP. Other band(s): none. Page 3 of 6 v.20.1 Updated 10/10/2018 Western Blot: GFP Antibody [NB ] - Lane 1: 293FT cells transfected with CDK4 dominant negative. Lane 2: 293FT cells poitive control. Load: 25 ug per lane. Primary antibody: GFP antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 (TM) rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 27 kda for GFP. Western Blot: GFP Antibody [NB ] - 50ng of GFP HRP conjugate. Western Blot: GFP Antibody [NB ] - Analysis using the FITC conjugate of NB Detection of Lane 1: GFP. Load: 50 ug per lane. Primary antibody: none. Secondary antibody: Fluorescein conjugated Anti-GFP at 1:1000 for 60 min at RT. Block: 1% BSA-TTBS for 30 min at RT. Predicted/Observed size: 28 kda, 28 kda.

5 Immunocytochemistry/Immunofluorescence: GFP Antibody [NB ] Page 4 of 6 v.20.1 Updated 10/10/2018 Immunoprecipitation: GFP Antibody [NB ] - IP analysis of GFP in HeLa cell lysate. Image courtesy of anonymous customer review.

6 Publications Barenz F, Kschonsak YT, Meyer A et al. Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry. Mol. Biol. Cell. Oct :00AM [PMID: ] (WB, Jellyfish) Lobs Ak, Schwartz C, Thorwall S, Wheeldon I. Highly multiplexed CRISPRi repression of respiratory functions enhances mitochondrial localized ethyl acetate biosynthesis in kluyveromyces marxianus. ACS Synth Biol. Oct :00AM [PMID: ] (WB, Jellyfish) Almohazey D, Lo YH, Vossler CV et al. The ErbB3 receptor tyrosine kinase negatively regulates Paneth cells by PI3K-dependent suppression of Atoh1. Cell Death Differ. May :00AM [PMID: ] (ChIP) Bassermann AG. Dissertation_Baumann_vorlaufig Thesis (Jellyfish) Lin Y, Fang ZP, Liu HJ et al. HGF/R-spondin1 rescues liver dysfunction through the induction of Lgr5(+) liver stem cells. Nat Commun Oct 27 [PMID: ] (ICC/IF, Mouse) Lo YH, Chung E, Li Z et al. Transcriptional Regulation by ATOH1 and its Target SPDEF in the Intestine. Cell Mol Gastroenterol Hepatol Jan 01 [PMID: ] (ChIP, Human) Jain A, Beutel O, Ebell K et al. Diverting CERT-mediated ceramide transport to mitochondria triggers Bax-dependent apoptosis. J. Cell. Sci. Nov :00AM [PMID: ] (Human) Brunk K, Zhu M, Barenz F et al. Cep78 is a novel centriolar protein involved in Plk4-induced centriole overduplication. J. Cell. Sci. May :00AM [PMID: ] Zangar RC, Daly DS, White AM et al. ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays J Proteome Res This publication used the Biotin conjugated form of this antibody (Cat# NB ). Olszak T, Neves JF, Dowds CM et al. Protective mucosal immunity mediated by epithelial CD1d and IL-10 Nature (IHC-Fr) This publication used the FITC conjugated form of this antibody (Cat# NB ). Olszak T, Neves JF, Dowds CM et al. Protective mucosal immunity mediated by epithelial CD1d and IL-10 Nature 2014 May 22 [PMID: ] (IHC-Fr) This citation used the FITC version of this antibody. Zangar RC, Daly DS, White AM et al. ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays J Proteome Res 2009 Aug [PMID: ] This citation used the Biotin version of this antibody. More publications at Page 5 of 6 v.20.1 Updated 10/10/2018

7 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF008 NB7160 NBP NBC Goat anti-rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Goat anti-rabbit IgG (H+L) Secondary Antibody [HRP] Rabbit IgG Isotype Control Recombinant Jellyfish GFP Protein Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: