Product Datasheet. TLR7 Antibody NBP SS. Unit Size: mg

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1 Product Datasheet TLR7 Antibody NBP SS Unit Size: mg Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Reviews: 3 Publications: 53 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/10/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NBP SS TLR7 Antibody Product Information Unit Size Concentration Storage Clonality Preservative Isotype Purity Buffer mg 1.0 mg/ml Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. Polyclonal 0.05% Sodium Azide IgG Protein G purified PBS Page 1 of 6 v.20.1 Updated 2/10/2019 Product Description Host Rabbit Gene ID Gene Symbol Species Immunogen Product Application Details Applications Recommended Dilutions TLR7 Human, Mouse, Rat This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids of human TLR7; GenBank no. AAF , AF245702_1. Cross-reactivity with mouse TLR7 shown. In human Ramos cells, additional bands are also seen. Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Proximity Ligation Assay, Immunofluorescence Western Blot 1-3 ug/ml, Flow Cytometry 2-5 ug/ 1x10^6 cells, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:10-1:2000, Proximity Ligation Assay, Flow (Intracellular), Immunofluorescence Application Notes Use in IHC-P (PMID: ), IHC (PMID: ), IP (PMID: ), and Dot Blot (PMID: ) shown in scientific literature. Use in Proximity Ligation Assay reported in scientific literature (PMID: ). Images Flow (Intracellular): TLR7 Antibody [NBP ] - Analysis using the FITC conjugate of NBP Staining of TLR7 in human monocytes using this antibody at 0.5 ug/10^6 cells. Shaded histogram represents cells alone, green represents rabbit IgG isotype control, red represents anti-tlr7 antibody.

3 Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP ] - Analysis using the Azide Free version of NBP Staining of Raw cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red). Image objective 40x. An antibody dilution of 1:10 was used. Page 2 of 6 v.20.1 Updated 2/10/2019 Immunohistochemistry-Frozen: TLR7 Antibody [NBP ] - analysis of TLR7 in acetone-fixed, frozen mouse ear skin section using anti-tlr7 antibody. Image from verified customer review. Immunohistochemistry-Frozen: TLR7 Antibody [NBP ] - analysis of frozen mouse ear skin tissue using anti-tlr7 antibody. Image from verified customer review. Western Blot: TLR7 Antibody [NBP ] - Analysis using the Azide Free version of NBP Detection of TLR7 in RAW cell lysate using this antibody.

4 Immunohistochemistry-Paraffin: TLR7 Antibody [NBP ] - Analysis using the Azide Free version of NBP Staining of human colon tissue using NBP (top) and an isotype control (bottom) at 5 ug/ml. Page 3 of 6 v.20.1 Updated 2/10/2019 Flow Cytometry: TLR7 Antibody [NBP ] - An intracellular stain was performed on THP-1 cells with TLR7 Antibody NBP (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5.0 ug/ml for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA , Thermo Fisher). Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP ] - Immunolocalization of TLR7 (A) and glial fibrillary acidic protein (GFAP) (B) in murine myenteric plexus. Most of the GFAP positive cells were also positive for TLR7 in the merged image (C, yellow staining). (Courtesy of Barajon I, et al., Journal of Histochemistry and Cytochemistry, 57(11): 1013-;1023, 2009.) Immunocytochemistry/Immunofluorescence: TLR7 Antibody [NBP ] - Ramos cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with TLR7 NBP at a 1:50 dilution for 1 hour at room temperature and detected with Dylight 488 (Green). Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with Dylight 550 (Red). Nuclei were detected with DAPI (Blue). Cells were imaged using a 40X objective.

5 Flow Cytometry: TLR7 Antibody [NBP ] - Analysis using the FITC conjugate of NBP Staining of TLR7 in TLR7/HEK 293 cells using NBP at 1 ug/10^6 cells. Green represents Vector/HEK 293 cells; red represents TLR7/HEK 293. Page 4 of 6 v.20.1 Updated 2/10/2019 Flow Cytometry: TLR7 Antibody [NBP ] - Analysis using the PE conjugate of NBP Staining of PBMCs fixed and permeabilized using the intracellular staining kit and stained with 1 ug of TLR7-PE conjugate (red) or matched isotype control ( green). Flow Cytometry: TLR7 Antibody [NBP ] - Analysis using the FITC conjugate of NBP Staining of TLR7 in Balb/c mouse splenocytes using this antibody. Green represents rabbit IgG isotype control ; red represents anti-tlr7 antibody. TLR7 Antibody [NBP ] - Analysis using the Azide Free version of NBP Staining of TLR7 in human PBMC using this antibody at 0.5 ug/ml. Shaded histogram is cells alone, green is rabbit IgG isotype control (NBP ), red represents anti-tlr7 antibody.

6 Publications Blanchard EL, Loomis KH, Bhosle SM et al. Proximity Ligation Assays for In Situ Detection of Innate Immune Activation: Focus on In Vitro-Transcribed mrna Mol Ther Nucleic Acids Nov :00AM [PMID: ] (PLA, Mouse) Blanchard EL, Loomis KH, Bhosle SM et al. Proximity ligation assays for in situ detection of innate immune activation: focus on in vitro transcribed mrna. Molecular Therapy - Nucleic Acids. Nov :00AM [PMID: ] (PLA, Mouse) Loomis KH, Lindsay KE, Zurla C et al. In Vitro Transcribed mrna Vaccines with Programmable Stimulation of Innate Immunity Bioconjug. Chem. Aug :00AM [PMID: ] (PLA, Mouse) Murayama Goh, Furusawa Nanako, Chiba Asako et al. Enhanced IFN-a production is associated with increased TLR7 retention in the lysosomes of palasmacytoid dendritic cells in systemic lupus erythematosus. Arthritis Research & Therapy 2017 [PMID: ] (Flow, Human) Milara Javier, Morell Anselm, Ballester Bea et al. Roflumilast improves corticosteroid resistance COPD bronchial epithelial cells stimulated with toll like receptor 3 agonist. RespiRatory Research 2015 [PMID: ] (IHC, WB, Human) Aravanis CV, Kapelouzou A, Vagios S et al. Toll-Like Receptors-2,-3,-4 and-7 Expression Patterns in the Liver of a CLP-Induced Sepsis Mouse Model. J Invest Surg. May :00AM [PMID: ] (IHC) Jouhi L, Mohamed H, Makitie A, Remes SM et al. Toll-like receptor 5 and 7 expression may impact prognosis of HPVpositive oropharyngeal squamous cell carcinoma patients Cancer Immunol. Immunother Aug 30 [PMID: ] (Human) To EE, Vlahos R, Luong R et al. Endosomal NOX2 oxidase exacerbates virus pathogenicity and is a target for antiviral therapy Nat Commun 2017 Jul 12 [PMID: ] (ICC/IF, Mouse) Zeng M, Nouri-Shirazi E, Guinet E, Nouri-Shirazi M. The genetic background influences the cellular and humoral immune responses to vaccines Clin. Exp Immunol. [PMID: ] (Flow, Mouse) Details: Citation using the Azide Free form of this antibody. Liu T, Xu ZZ, Park CK et al. Toll-like receptor 7 mediates pruritus. Nat Neurosci. [PMID: ] Details: Citation using the Azide Free form of this antibody. Larsson O, Tengroth L, Xu Y et al. Substance P represents a novel first-line defense mechanism in the nose. J. Allergy Clin. Immunol Feb 20 [PMID: ] (Flow, ICC/IF, IHC, Mouse) Jeon YK, Kim CK, Koh J et al. Pellino-1 confers chemoresistance in lung cancer cells by upregulating ciap2 through Lys63-mediated polyubiquitination. Oncotarget. Jul :00AM [PMID: ] (DB, Human) More publications at Page 5 of 6 v.20.1 Updated 2/10/2019

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