Product Datasheet. Luciferase Antibody (Luci ) NB Unit Size: 0.1 ml. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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1 Product Datasheet Luciferase Antibody (Luci ) NB Unit Size: 0.1 ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Reviews: 2 Publications: 15 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 2/20/2019 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB Luciferase Antibody (Luci ) Product Information Unit Size Concentration Storage Clonality 0.1 ml 1.0 mg/ml Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. Monoclonal Clone Luci Preservative Isotype Purity Buffer 0.02% Sodium Azide IgG1 Kappa Protein A or G purified PBS Page 1 of 4 v.20.1 Updated 2/20/2019 Product Description Host Species Reactivity Notes Specificity/Sensitivity Immunogen Mouse Firefly Photinus pyralis (North American firefly). Luciferase recombinant reactivity in Mouse and Rat reported by a customer review. Specific for Luciferase, recognizing a peptide consisting of the first 258 amino acids. Further epitope mapping has not been done at this time. Luciferase protein from Photinus pyralis (North American firefly). [UniProt# P08659] Product Application Details Applications Western Blot, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Western Blot 1:1000, Flow Cytometry 1:200, Immunohistochemistry 1:100-1:1000, Immunocytochemistry/Immunofluorescence 1:100-1:1000, Immunohistochemistry-Paraffin 1:100-1:1000 Application Notes Western blot has been tested with Drosophila embryos. Purified Luciferase protein and Luciferase expressed in Drosophila adult co-migrate on Western blots with a band seen at ~61 kda, representing Luciferase. Flow Cytometry has been reported in a customer review.

3 Images Western Blot: Luciferase Antibody (Luci ) [NB ] - WB analysis of NB on Luciferase Protein (NB ) Page 2 of 4 v.20.1 Updated 2/20/2019 Immunohistochemistry: Luciferase Antibody (Luci ) [NB ] - Detection of Luciferase expression in CD34+ cells by Immunohistochemistry. Cytospin slides prepared from transduced CD34+ cells after 3 days of culture were stained with monoclonal anti- Luciferase antibody. Luciferase-positive cells have green cytoplasm; nuclei stained with DAPI are blue. Nontransduced, cultured CD34+ cells were used as a negative control. Original magnification, 40X. Wang, X. et al., Dynamic tracking of human hematopoietic stem cell. Blood. 102 (10): , 2003.

4 Publications Galisova A, Herynek V, Swider E et al. A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging. Mol Imaging Biol Aug :00AM [PMID: ] (IHC-P, Firefly) Cunningham D, Zhang Q, Liu S et al. Interleukin-17 promotes metastasis in an immunocompetent orthotopic mouse model of prostate cancer. Am J Clin Exp Urol. Jun :00AM [PMID: ] (IHC-P, Mouse) Tang X, Molina M, Amar S. p53 short peptide (p53pep164) regulates lipopolysaccharide-induced tumor necrosis factor-alpha factor/cytokine expression. Cancer Res Feb 1 [PMID: ] (Firefly) Sayed D, Yang Z, He M et al. Acute Targeting of General Transcription Factor IIB Restricts Cardiac Hypertrophy via Selective Inhibition of Gene Transcription. Circ Heart Fail Nov 14 [PMID: ] (WB, Mouse, Rat) Wang K, Liu S, Wang J et al. Transcriptional regulation of human USP24 gene expression by NF-kappa B. J Neurochem Nov 29 [PMID: ] (WB) Wilber A, Linehan JL, Tian X et al. Efficient and stable transgene expression in human embryonic stem cells using transposon-mediated gene transfer. Stem Cells 2007 Nov [PMID: ] (ICC/IF) Li JF, Bush J, Xiong Y et al. Large-scale protein-protein interaction analysis in Arabidopsis mesophyll protoplasts by split firefly luciferase complementation. PLoS One 2011 [PMID: ] (WB, Firefly) Love Z, Wang F, Dennis J et al. Imaging of mesenchymal stem cell transplant by bioluminescence and PET. J Nucl Med;48(12): Dec. [PMID: ] (IHC, Firefly) Aleksic, T et al. Cellular immune reaction in the pancreas is induced by constitutively active IkappaB kinase-2. Gut;56 (2): Feb. [PMID: ] Zhou, W et al. Leaky scanning and reinitiation regulate BACE1 gene expression. Mol Cell Biol;26(9): May. [PMID: ] (WB, Firefly) Schmeisser MJ, Baumann B, Johannsen S et al. IkB Kinase/Nuclear Factor?B-Dependent Insulin-Like Growth Factor 2 (Igf2) Expression Regulates Synapse Formation and Spine Maturation via Igf2 Receptor Signaling. J Neurosci;32 (16): Apr 18. [PMID: ] (ICC/IF, Mouse) Iijima K, Zhao L, Shenton C, Iijima-Ando K. Regulation of energy stores and feeding by neuronal and peripheral CREB activity in Drosophila. PLoS One;4(12):e Dec 30. [PMID: ] (WB) More publications at Page 3 of 4 v.20.1 Updated 2/20/2019

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Products Related to NB HAF007 NB720-B NBP mg NB PE Goat anti-mouse, IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Rabbit anti-mouse IgG (H+L) Secondary Antibody [Biotin] Mouse IgG1 Kappa Light Chain Isotype Control (P ) Luciferase Antibody (Luci ) [PE] Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: