OIE Reference Laboratory Reports Activities

Transcription

1 OIE Reference Laboratory Reports Activities Activities in 2016 This report has been submitted : :39:43 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Foot and mouth disease Address of laboratory: USDA, APHIS,VS, STAS, NVSL-FADDL PO Box 848, Greenport, NY, UNITED STATES OF AMERICA Tel.: Fax: address: consuelo.carrillo@aphis.usda.gov Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Beverly J. Schmitt, Director National Veterinary Services Laboratories, STAS, VS, APHIS, USDA Name (including Title and Position) of OIE Reference Expert: Dr. Consuelo Carrillo VMO-Senior Animal Health Advisor DSS-FADDL-STATS-VS-NVSL-APHIS-USDA Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,

2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally ELISA (NSP, 3ABC) 1,030 6 VIAA (AGID) Virus neutralization 1 12 Direct diagnostic tests Nationally Internationally Cell Culture (IBRS-2 cells) Cell Culture (Lamb Kidney Primary Cells) Real-time RT-PCR 1, ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2016

3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Proficiency test panels of FMD for real time RT- PCR FMD rrt- PCR Complete sets of 12 samples plus controls Africa Americas Asia and Pacific Europe Middle East FMDV antigens FMD antigen ELISA FMD 0 8 vial of 1 ml each 1 Africa Americas Asia and Pacific Europe Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? OIE Reference Laboratory Reports Activities,

4 Name of the new test or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Electro Immune Transferrence Blotting Test 1. Developing reagents for the OIE recommended confirmatory test for detecting antibodies against non-structural proteins of FMDV in sera from infected animals using the Enzyme Immune Transfer Blotting technique (EITB). This test has not been validated yet, it is currently in the phase of bench validation FMDV vaccinated Bovine sera 2. Developing reagents for standardization of the vaccine matching technique using sera from vaccinated bovines in virus neutralization test, not yet validated. Currently in the phase of bench validation Liquid Phase Blocking ELISA Test 3. Validation of an in house produced liquid phase blocking ELISA (LPBE) to detect and characterize antibodies against FMDV structural proteins ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided PANAMA Working with OIRSA countries to enhance vesicular surveillance diagnostic capacity. In particular work has been done with LADIVES. Remote and on location. JORDAN Conducted a workshop in collaboration with the Jordan University of Science and Technology to enhance the immunohistochemistry capabilities in the region, to include diagnostics for FMD. on location ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2016

5 Title of the study Duration Purpose of the study Partners (Institutions) OIE Member Countries involved other than your country Evaluation of syndromic surveillance for transboundary disease in the Tigray region of Ethiopia NOV 2016 SEP 2017 Evaluate syndromic surveillance training Univeristy of Georgia (PI), Mekelle University ETHIOPIA ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 4 Schutta C, Barrera J, Pisano M, Zsak L, Grubman MJ, Mayr GA, Moraes MP, Kamicker BJ, Brake DA, Ettyreddy D, Brough DE, Butman BT, Neilan JG. Multiple efficacy study of an adenovirus-vectored foot-and-mouth disease virus serotype A24 subunit vaccine in cattle using homologous challenge. Vaccine. 2016; 34(27): Duque H, Naranjo J, Carrillo C, Burbano A, Vargas J, Pauszek L, Olesen I, Sanchez-Vazquez MJ, Cosivi O, Allene RM. Protection induced by a commercial bivalent vaccine against Foot-and-Mouth Disease in 2010 field virus from Ecuador. Vaccine. 2016; 34(35): Rhyan J, McCollum M, Gidlewski T, Shalev M, Ward G, Donahue B, Artz J, Stenfeldt C, Mohamed F, l P, Deng M, Metwally S, McKenna T, Salman M. Foot-and-mouth disease in a small sample of experimentally infected pronghorn (Antilocapra americana). J Wildl Dis Jianfa Bai, Amaresh Das, Xiju Shi, Qing Sun, Guiping Ma, Qin Wang, Gary Anderson, Jishu Shi, Wei Jia, Lu Xu, Xuming Liu, Yanhua Liu, Lalitha Peddireddi (2016). A multiplex real-time PCR panel assay for simultaneous detection and differentiation of 12 common swine viruses. J. Virol. Methods. 236; 258:265. b) International conferences: 2 Comisión Sudamericana para la Lucha Contra la Fiebre Aftosa (COSALFA) 43 meeting in Punta del Este (Uruguay), in April 15-19, 2016 EuFMD: The practice of innovation October 2016, Cascais (Portugal) c) National conferences: 1 The United States Animal Health Association (USAHA) and the American Association of Veterinary Laboratory Diagnosticians (AAVLD), USAHA/AAVLD 59th, October 2016, Greensboro, rth Carolina (US). Presentation title: FADDL Update for 2016 d) Other: (Provide website address or link to appropriate information) 0 OIE Reference Laboratory Reports Activities,

6 ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 1 b) Seminars: 1 c) Hands-on training courses: 2 d) Internships (>1 month): 0 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country a Panama OIRSA-LADIVES 3 b Panama OIRSA-LADIVES 17 c Panama OIRSA-LADIVES 3 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO diagnostics ISO proficiency Certificate scan (PDF, JPG, PNG format) ISO pd.pdf ISO pd.pdf 16. Is your laboratory accredited by an international accreditation body? 6 OIE Reference Laboratory Reports Activities, 2016

7 Test for which your laboratory is accredited Antigen Capture ELISA Virus Isolation Virus Neutralization Test 3ABC ELISA Real Time RT-PCR VIAA-AGID Accreditation body 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Chapter 1.1.4) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented 11th OIE/FAO FMD Reference Laboratory Network 11/16 France short communication Foreign Animal Disease Diagnostic Laboratory (FADDL) NVSL-VS-STAS-APHIS USDA ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? OIE Reference Laboratory Reports Activities,

8 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. FMD and SVD combined proficiency test scheme (PTS) participant 4 Organized by: the European Union Reference Laboratories (EURL) and FAO World Reference laboratory (WRL) FMDV proficiency test PANAFTOSA participant 4 Organized by: PANAFTOSA, Pedro Leopoldo-Brazil 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Organized: Foot and Mouth Disease Proficiency Tests (PT) and Evaluation 45 Africa Americas Asia and Pacific Europe Middle East 8 OIE Reference Laboratory Reports Activities, 2016

9 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: N/A OIE Reference Laboratory Reports Activities,