National Veterinary Institute (NVI)

Transcription

1 National Veterinary Institute (NVI) ESAYAS GELAYE November 2014, Addis Ababa

2 Biotechnology: Experiences and Potentials

3 Outline NVI Introduction Vaccines production RD activities and facilities Opportunities Challenges Recommendation

4 NVI was established in 1964GC (1956EC) Debre zeit - 45 km south of Addis Ababa Celebrated 50 years of anniversary

5 The institute is Governed by the Board of Directors Working on cost-recovery base, with full financial and administrative autonomy Certified with QMS ISO 9001:2008 since 2006 Accredited with ISO/IEC 17025:2005 Established Enterprise Resource Planning Host AU-PANVAC

6 Definition: Biotechnology the use of living organisms in industrial processes particularly in agriculture, food processing, and medicine. (Biotechnology, David P. Clark) Biotechnology is the use of living systems and organisms to develop or make useful products, or "any technological application that uses biological systems, living organisms or derivatives thereof, to make or modify products or processes for specific use. (Wikipedia)

7 PRODUCED VACCINES oviral vaccines: Ruminants - Rinderpest - Peste des Petits Ruminants (PPR) - Sheep & Goat Pox (SGP) - Lumpy Skin Disease (LSD) - Foot & Mouth Disease (FMD) serotype O, A and SAT2 Equines - African Horse Sickness (AHS) serotype 2, 4 and 9 Camel - Camel Pox (CMP) Poultry - Newcastle [HB1, Lasota, Thermostable & Inactivated] - Fowl pox - Infectious Bursal Disease (Gumboro)

8 obacterial vaccines: Ruminants - CCPP - CBPP [T144 and T1SR] - Anthrax - Blackleg - Bovine Pasteurellosis - Ovine Pasteurellosis Poultry - Fowl Typhoid Produced around 153x10 6 doses of vaccines in 2006 fiscal year o Working to produce Marek s and Rabies vaccine

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10 NVI doesn t produce Subunit/DNA/Recombinant vector vaccines

11 ISO 9001:2008

12 Fermenter and Inactivator with a capacity of 2,500 liters

13 Production Lab (under construction) plan to implement GMP

14 R & D Labs Laboratory Facilities Bacteriology Lab Serology Lab Virology Lab Molecular Biology Lab Nutrition Analysis Lab Lyophilization (small capacity) Washing and Sterilization Room

15 Serology Lab ELISA (FMD, CCPP, CBPP, AHS, IBD, etc) CFT (CCPP) HA (NCDV) HAI (NCD Ab) AGID (IBD) RBPT (Brucellosis) Accreditation for ISO/IEC 17025:2005 for four serology tests. - 3-ABC ELISA for FMD - C-ELISA for CBPP and CCPP - RBPT for Brucella Plan to increase the scope by 20% each year

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17 ISO/IEC 17025:2005

18 Bacteriology Lab. Bacterial culture Bacterial isolation and identification Biochemical test Antimicrobial sensitivity test Lyophilization of isolates and storage in the repository

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20 Virology Lab. o Animal Cell culture: primary cell: - Calf kidney - Lamb kidney - Chicken Embryo Fibroblast monolayer cell line: - VERO - BHK21 o Incubation at 37ºC with 5% CO2 o Cell storage using Liquid Nitrogen o Regular checking of cell viability and sterility

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22 Cont o Pathological samples processing using Biosafety C. II o Virus isolation using susceptible cell culture o Observation of CPE using Inverted microscope o Preserve isolates using ultra freezer

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24 Molecular Biology Lab. Nucleic acid extraction (DNA/RNA) Conventional PCR RT-PCR Gel-electrophoresis real time PCR Purification of PCR product Quantification of PCR product (NanoDrop) Preparation of PCR products and Sequencing of gene/gene fragment

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28 Cont Sequence analysis (Bioinformatics) o editing and contig formation/vector NTI o blastn and protein translation/ncbi & ExPASy o multiple alignment/bioedit o phylogenetic tree construction/mega5.1 PLoS ONE, 2013, 8 (10): e75971

29 Opportunities Good working environment Better Lab facilities Experienced and trained experts Strong research collaboration with National and International research institutes Lab animals availability Existence of animal experiment units Product Development (PD) Lab - established by NVI, PANVAC and GALVMed.

30 Lab animals

31 Research collaboration o IAEA - TCP and CRP, AUSTRIA o FAO/IAEA, AUSTRIA o CIRAD, FRANCE o GALVMed, UK o Pirbright, UK o AU/PANVAC NAHDIC EIAR (NARF and ARTP) Universities Regional Vet. Diagnostic Labs International National

32 Achievements Production of vaccines and diagnostic kits Improved capacity of doing applied research Improvement of existing vaccines Development of new vaccines Increased collaboration on research projects Increased capacity of expertise and infrastructure Future plan to be performed by 2015/16: Cloning facilities Proteomics Bioinformatics center

33 Contributed for the eradication of Rinderpest (using Thermostable vaccine)

34 Challenges/Constraints Research fund limitations Shortage of major high tech lab equipments Limitation of consumables and reagents Capacity building training on specific technique Tax problem Recommendations Capacity building training and Lab facilities Allocate Research fund Organize workshop Evaluate Biotech course curriculum

35 Committed in producing and supplying quality veterinary vaccines Thereby contributing for agricultural productivity and food security

36 Environment-friendly Inst.

37 Thank U All እናመሰግናለን