CURRICULUM VITAE. Name: Johanna Jeanine Sloss (Fryman) Telephone:

Transcription

1 Name: Johanna Jeanine Sloss (Fryman) Telephone: CURRICULUM VITAE Education: Devry University, Keller Graduate School of Management, MBA, August present Southern Illinois University, Carbondale, IL, B. A. Microbiology, May 2000 Olney Central College, Olney, IL, Assoc. of Arts, Assoc.of Science, May 1995 Certifications: Molecular Biology Specialist, MB (ASCP) CM, 8/2009 Professional Experience: Research and Development, Molecular Pathology Department, Marshfield Clinic, Marshfield, WI, 11/05-present Clinical Laboratory Technician, Molecular Biology Department, Marshfield Clinic, Marshfield, WI, 04/02-11/05 Research Technician I, Howard Hughes Medical Institute, University of Cincinnati, Department of Molecular Genetics, Cincinnati, OH, 1/02-4/02 Research Assistant, University of Cincinnati, Department of Molecular Genetics, Cincinnati, OH, 2/01-1/02 Research Technician II, Washington University School of Medicine, Genome Sequencing Center, St. Louis, MO, 5/00 12/00 Student Researcher, Dr. Laurie Achenbach/Dr. John Coates, Microbiology Department, Southern Illinois University, Carbondale, IL, 8/98 5/00 Field Researcher, Monsanto, Grand Island, NE, 5/98 8/98 Field Researcher, Alvey Labs, Mt. Carmel, IL, 5/97 11/97 Other: SIUC Microbiology Student Organization, President, 5/99 5/00 Undergraduate Student Advisory Committee Microbiology Representative Molecular Pathology Contract Speaker, Wisconsin College of American Pathologists Molecular Inspector, 2010 Independent Consultant, Molecular, 2010 ASCP Local Representative for the Midwest Region, 9/2010 Laboratory duties: Supervised and trained new employees Supervised and trained individuals in clinical applications Cost accounting Quality Control parameters Advise on marketing molecular assays Molecular biology CAP preparation and inspection liaison Supervised Medical Technology student rotation in department

2 Sloss 2 Evaluated Medical Technology student progress Collaborate with other departments in molecular development Prepared work schedules for department Prepared and updated laboratory protocols Experience with laboratory information systems Development of new molecular based clinical tests Provide input to identify opportunities for process improvement Troubleshooting and problem resolution of clinical tests Test packet preparation of clinical tests Maintained inventory and ordering Communicate with Clinical Sales Representatives Negotiate with Clinical Sales Representatives Supervised and trained individuals in manual DNA sequencing, PCR and mouse husbandry skills Lead Technician- Cobas Amplicor System Laboratory Skills: Polymerase Chain Reaction Prokaryotic cloning Mouse Husbandry Skills Manual DNA sequencing Automated DNA sequencing protocols Primer design Media and reagent preparation Computer-aided sequence alignment RNA isolation Tissue culture Southern Blot Western Blot Northern Blot cdna Light Cycler applications Cobas Amplicor Instrument System Real-Time Instrument Systems Automated DNA and RNA extraction systems Clinical tests: Human papillomavirus identification Hepatitis C quantitative PCR Hepatitis C qualitative PCR HIV quantitative PCR Chlamydia trachnomatis PCR Neisseria gonorrhea PCR Factor V Lieden gene mutation Prothrombin gene mutation Hepatitis B quantitative PCR Cystic Fibrosis Herpes simplex I & II PCR

3 Sloss 3 Bordetella pertussis and B. parapertussis PCR Borrelia burgdorferi PCR Varicella zoster PCR Hepatitis C genotyping Alpha-1-Anti-trypsin PCR Clostridium difficile PCR Methicillin-resistant Staphylococcus aureus PCR Test Validation: Hepatitis B quantitative PCR Hepatitis C quantitative PCR HIV quantitative PCR Cystic Fibrosis Bordetella pertussis and B. parapertussis PCR Alpha-1-Anti-trypsin PCR Extraction automation for various sample types Various DNA extraction changes for current testing platforms Lyme Disease Western blot Herpes simplex 1& 2 PCR Clostridium difficile PCR Methicillin-resistant Staphylococcus aureus PCR KRAS mutation analysis (Codons 12, 13, 61) Memberships in Professional Organizations: American Society of Microbiology American Society for Clinical Pathology Association for Molecular Pathology Clinical and Laboratory Standards Institute American Society for Clinical Laboratory Science Publications: Uphoff, T. and J. Sloss Molecular Diagnostic Testing for Varicella-Zoster Virus Infections Expands to Swab Samples from Skin and Mucosal Lesions. Marshfield Clinic Laboratory News 33:7. Uphoff, T. and J. Sloss Molecular Diagnostic Testing for Herpes Simplex Virus Infections Expands to Swab Samples from Skin and Mucosal Lesions. Marshfield Clinic Laboratory News 33:4. Uphoff, T. and J. Sloss New Testing Methods Expand the Reportable Range for Hepatitis B and Hepatitis C Virus Quantitative Assays. Marshfield Clinic Laboratory News 31:7. Uphoff, T. and J. Sloss Laboratory Testing for Alpha-1-Antitrypsin Deficiency [AATGENO]. Marshfield Clinic Laboratory News 30:6. Dokken, C., J. Fryman, C. Hipke, B. Voelker, K. Reed, B. Krawisz. October 27, Detection of Human Papillomaviruses and Its Usefulness in Interpreting Results

4 Sloss 4 of Cervical Smears. Marshfield Clinic Laboratory News, 25: 5 Achenbach, L. A., U. Michaelidou, R. A. Bruce, J. Fryman, and J. D. Coates Dechloromonagitata gen. nov., sp. nov. and Dechlorosomaillum gen. nov., sp. nov., two novel environmentally dominant (per)chlorate-reducing bacteria and their phylogenetic position. International Journal of Systematic and Evolutionary Microbiology 51: Kazusa DNA Research Institute, The Cold Spring Harbor and Washington University Sequencing Consortium, The European Union Arabidopsis Genome Sequencing Consortium & Institute of Plant Genetics and Crop Plant Research (J. Fryman among 128 authors) Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana. Nature 408: GeneBank Human Genome Project, Washington University Genome Sequencing Center: AC , AC , AC , AC , AC , AC , AC , AC , AC Presentations: Sloss, J.J., M. F. Vandermause, J.K. Meece, and T.S Uphoff. June Evaluation of Three Multiplex RT-PCR Assays for Influenza A, Influenza B, and Respiratory Syncytial Virus Detection. Marshfield Clinic 2010 Medical Education Day, Marshfield, WI. Sloss, J.J., M. F. Vandermause, J.K. Meece, and T.S Uphoff. May Evaluation of Three Multiplex RT-PCR Assays for Influenza A, Influenza B, and Respiratory Syncytial Virus Detection American Society for Microbiology General Meeting, San Diego, CA. Uphoff, T., J. Sloss, M. E. Stemper, T. Novicki, and T. Fritsche. April Comparison of three real-time PCR assays for toxigenic Clostridium difficile with a two-tiered rapid immunochromatography algorithm. 26 th Annual Clinical Virology Symposium, Daytona, FL. Sloss, J.J. September DNA Technology in the Clinical Laboratory. 16 th Annual Symposium Review. Molecular Pathology Department, Marshfield Labs. Marshfield, WI Fryman, J., L. A. Achenbach, U. Michaelidou, R. A. Bruce, and J. D. Coates. March, Phylogenetic analysis of dissimilatory (per)chlorate-reducing bacteria and development of molecular probes Cellular and Molecular Biology Symposium, Southern Illinois University, Carbondale, IL. Michaelidou, U., L. A. Achenbach, S. O Connor, J. Fryman, and J. D. Coates The diversity and ubiquitous microbiology of perchlorate reduction. American Water Works Association meeting, Albuquerque, NM.

5 Sloss 5 Relevant Science Course Work: Elementary Microbiology Basic Genetics Principles of Microbiology Medical Microbiology Undergraduate Independent Research Prokaryotic Diversity Diagnostics and Applied Microbiology Human Gross Anatomy Organic Chemistry Inorganic Chemistry Molecular Biology Biotechnology Genetics of Bacteria and Viruses Molecular Biology of Microorganisms Lab Anatomy and Physiology Advanced Anatomy and Physiology Relevant Business Course Work: Managerial Application of Information Technology Legal, Political and Ethical Dimensions Leadership and Organizational Behavior Business Economics Marketing Business Statistics

6 References: Available upon request. Sloss 6