The use of a Bacteria Specific Rapid Metabolic Assay in the detection of total bacterial counts in Fluidic samples

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1 The use of a Bacteria Specific Rapid Metabolic Assay in the detection of total bacterial counts in Fluidic samples Dr Andrew Kemp PhD Principle Scientific Officer Q Technologies Group

2 Every Generation Needs A Revolution Thomas Jefferson

3 PROFILE-1 3

4 History Developed by leading Immunologists for the US military. Early warning for bio warfare for field soldiers Used in the Gulf war Now used by the FDA and EPA Refined and adapted for civilian use, launched May 15, 2015

5 Why is this test needed?

6 ATP Adenosine Triphosphate Stores Energy All Animal and plant life have ATP 1 Human cell has 1 Billion ATP molecules Over 100Kg ATP produced in the Human Body per day! Without ATP life would not exist

7 ATP Sources Total ATP Somatic Free Bacterial

8 Somatic Cells All cells with the exception of Germ cells (reproductive cells) are Somatic cells The human skin is made up of Somatic cells SOMA = Creek for Body

9 BSRMA - Test Components Sample (Hand or Hard Surface swab) Somatic Cell Releasing Agent (SRA - Phage lytic enzyme) Bacterial Cell Releasing Agent (BRA - Phage lytic enzyme) Luciferin-Luciferase (LL) Profile One Bacteria Monitor 9

10 Add 0.5ml of Water for Injection into Pipette Swab surface with Water for Injection Drain swab into Pipette Take 50 micro litres from Pipette and squirt into Filtravette.. ATP Bacteria Somatic cells 0.45mm filter

11 To remove Somatic ATP. Add 3 drops of Somatic Cell Releasing Agent (SRA) into Filtravette. ATP Bacteria Somatic cells 0.45mm filter

12 Use Pressure syringe to force non- microbial ATP through filtravette out to absorbent pad below. Bacterial ATP remains within the bacterial cell envelope Non-microbial ATP collects in absorbent pad Absorbant pad

13 Add 3 additional drops of SRA Use Pressure syringe to force non- microbial ATP through Filtravette out to absorbent pad below. More SRA (somatic cell releasing agent) washes residual non-bacterial ATP through filter to absorbent pad Absorbant pad

14 Place Filtravette into draw of Profile One To separate ATP from bacterial cell, add 2 drops of Bacterial Cell Releasing Agent (BRA) To make ATP luminesce add 50 micro litres of L.L. solution - Mix. Close draw and view reading within 60 seconds L / L BRA

15 The underlying principle of microbial ATP bioluminescence is that there is a quantitative relationship between the amount of microbial ATP in a sample and the viable microbial plate count. Relative Light Unit (Microbial ATP) Colony Forming Units (Bacteria) 15

16 Benefits Results in real time Provides accurate counts within 5 mins. Tests & sanitizing protocols can be repeated until preferred levels are obtained. Suitable for training of internal staff

17 Previously missed contamination levels Orthopaedic OR

18 Previously missed contamination levels hands

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20 Previously missed contamination levels Surgical Skin prep

21 Urine and CSF testing Pilot study 100 urines samples were tested using microscopy, blinded and then tested again using BRSMA. 2 samples were unsuitable for microscopy, but were tested with BSRMA, showing bacterial presence of 103,212 and 949,631 CFU s per 50ul. Culture was not performed in 16 samples after microscopy, however when tested with the BSRMA showed between 21,688 and 949,943 CFU per 50ul. In 12 samples there was no significant growth at Culture, however, BSRMA showed between 54,901 and 954,815 per 50ul.

22 We Should Not Be Afraid To Follow The Truth Wherever It May Lead Us Thomas Jefferson

23 Questions