Microgen C. difficile latex test

Transcription

1 C. difficile latex test A Rapid latex agglutination test for the confirmation of C. difficile directly from a selective medium. Clostridium difficile has a well documented role in pseudomembraneous colitis and diarrhoea associated with antibiotics. Evidence has also implicated C. difficile in colitis not induced by antibiotics, exacerbations of chronic inflammatory bowel disease and postoperative diarrhoea. Many laboratories rely on the detection of C. difficile cytotoxin to diagnose infection. However, investigations have shown that the detection of faecal cytotoxin is not always consistent with active infection. This, plus the fact that some laboratories do not have the specialised facilities that are required to detect faecal cytotoxin, has highlighted the continuing need for traditional culture methods. C. difficile provides a simple and specific test for confirmation. Rapid identification confirmed within 2 minutes Easy to use High sensitivity and specificity

2 C. difficile latex test Traditional methods for identification of C. difficile include GLC and cytotoxin detection. However, GLC is expensive in terms of time, equipment and cost per test and the link between presence of cytotoxin and symptoms is inconsistent. The test latex particles in C. difficile are coated with antibodies specific for C. difficile somatic antigens, resulting in a highly specific test with a high predictive negative value. The test maybe be performed from all recognised C. difficile isolation media. The simple-to-use kit gives clear cut results, contains a positive control, has a long shelf-life, and offers the confidence of high sensitivity and specificity. Ordering Information: Code Description Size Storage M41CE C. difficile 50 tests 2-8 C Reagents/Kit: Reagents Product Code Detects Quantity/Size C. difficile latex reagent M41a Positive control M41b 0.85% Isotonic saline M40 Latex particles coated with rabbit antibodies to C. difficile antigens Suspension of inactivated C. difficile antigens reactive with test latex reagent Sample preparation solution 0.5ml 5.0ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-094-EN revision 1.

3 Campylobacter latex test A rapid test for the identification and confirmation of enteropathogenic, thermophilic Campylobacter grown on selective media. Detects Campylobacter from solid selective media Permits the early detection of Campylobacter gastro-enteritis, enabling appropriate therapy to begin sooner Is highly specific so that identification can be reliably formed on the basis of the test alone Campylobacter gastro-enteritis is most prevalent in young children, but outbreaks are also common in adults. In temperate climates the incidence of Campylobacter gastro-enteritis peaks in summer and early autumn, whereas in tropical climates peak incidences may occur in the cooler months. Campylobacter infection may be treated with antibiotics but in the majority of cases, due to the length of time required by traditional detection methods, the acute phase has passed by the time a laboratory diagnosis is communicated to the physician. Rapid identification confirmed within 2 minutes Easy to use High sensitivity and specificity

4 Campylobacter latex test Campylobacter rapid test detects: C. jejuni C. jejuni subsp. doylei C. coli C. upsaliensis C. laridis C. fetus Ordering Information: Code Description Size Storage M46CE Campylobacter 50 tests 2-8 C Reagents/Kit: Reagents Product Code Contents Quantity/Size Campylobacter latex reagent Control latex reagent Positive control M46a M46b M46c 0.85% Isotonic saline M46d Latex particles coated with rabbit antibodies to Campylobacter antigens Latex particles coated with non-specific rabbit immunoglobulins Suspension of inactivated Campylobacter antigens reactive with test latex reagent and non-reactive with control latex reagent Sample preparation solution 1.0ml 5.0ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-096-EN revision 1.

5 E.coli O157 latex test A rapid test for the identification and confirmation of E. coli serogroup O157 grown on selective solid media. E. coli O157, one of a group of E. coli serotypes known as EHEC are the causative agents of Haemorrhagic colitis (HC), Haemolytic Uraemic Syndrome (HUS) and community acquired diarrhoea. Sorbitol MacConkey medium is the recommended screening agar for E. coli O157, as the strain is unable to ferment sorbitol. However, other sorbitol-negative Gram-negative rods also grow on sorbitol MacConkey agar. As a result of this, a specific rapid confirmation method is required. method and other commercially available latex tests have been shown to suffer from cross reactions with other enteric organisms such as E. hermanii, which antigenically and biochemically resembles E. coli O157, resulting in false positive results. The conventional method for confirmation of E. coli O157 serotypes utilises specific antisera in a standard agglutination test. However, this Rapid identification confirmed within 2 minutes Easy to use High sensitivity and specificity

6 E.coli O157 latex test E. coli O157 is an easy-to-use one step, latex kit which gives results in under two minutes and specifically confirms E. coli O157 directly from the selective agar, even in mixed culture. E. coli O157 gives strong and specific results within two minutes and comes complete with a control latex which can be easily compared with test results to confirm non-specific reactions. E. coli O157 demonstrated no cross reactions when tested against a panel of 161 E. coli O serotypes and 56 H serotypes, and shows strong specific agglutination. Ordering Information: Code Description Size Storage M44CE E. coli O tests 2-8 C Reagents/Kit: Reagents Product Code Detects Quantity/Size E.coli O157 latex reagent Control latex reagent M44a M44b 0.85% Isotonic saline M40 Latex particles coated with rabbit antibodies to E. coli O157 Latex particles coated with rabbit antibodies non-reactive to E. coli O157 Sample preparation solution 5.0ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-093-EN revision 1.

7 Legionella rapid A rapid latex agglutination test providing results in two minutes directly from a selective medium for the confirmatory identification of: Legionella pneumophila Serogroup 1 and Serogroup 2-15 Non-L. pneumophila species of clinical significance grown on selective media The Legionella rapid test is suitable for organisms derived from patients with suspected Legionella pneumonia or from environmental sources. Integrates into laboratory work flow improving efficiency Requires no special equipment or training 100% Specificity and Sensitivity compared to traditional culture methods Detects Legionella pneumophila Serogroup 1 and 2-15, together with 10 of the most clinically relevant species Compliant with ISO Water Quality Detection and enumeration of Legionella Individual reagents may be purchased separately Rapid identification confirmed within 2 minutes Applicable to both clinical and environmental testing

8 Legionella rapid The latexes used in Legionella are coated with immunoglobulins which have been specially processed to minimize issues with potentially cross reacting organisms thereby eliminating the need for a control latex. Legionella pneumophila identifies a range of non-l. pneumophila species of clinical significance with high specificity and no crossreaction with L. pneumophila. Detects the following species: L. pneumophila Serogroup 1 and 2-15 L. micdadei L. bozemanii I & II L. dumoffii L. longbeachae I & II L. jordanis L. gormanii L. anisa L. feelei Ordering Information: Code Description Size Storage M45CE Legionella 50 tests 2-8 C Reagents/Kit: Reagents Product Code Detects Quantity/Size Reagent 1 Reagent 2 Positive control M45ACE M45BCE M45CCE L. pneumophila Serogroup 1 L. pneumophila Serogroup 2-15 Positive Control, reactive with test reagents 1, 2-15 and species 1.0ml Reagent species M45DCE 10 commonly isolated clinically significant species If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-098-EN revision 1.

9 Listeria latex test A rapid test for the identification and confirmation of Listeria spp. from colonies grown on selective media. Listeria spp. are commonly found in soil and are transferred onto vegetable matter. Foods either derived from vegetables or which are derived from grazing animals and which are exposed to minimal processing i.e. dairy products, are the most common vector for the transmission of Listeria spp. to humans. There are 6 species commonly isolated, including Listeria monocytogenes which has been associated with human disease. L. monocytogenes is an opportunistic pathogen in that it will only cause disease in people with certain predisposing factors such as the immunosuppressed and pregnant women. Rapid identification confirmed within 2 minutes Easy to use High sensitivity and specificity Once isolated on agar plates, colonies resembling Listeria spp. are tested to confirm that they belong to that genus. Other organisms may be isolated from food samples which may resemble Listeria spp. To confirm the presence of Listeria spp. a number of tests are normally used including aesculin hydrolysis, oxidase, catalase, motility (differential at 25 C and 37 C). Microgen Listeria latex was developed as an alternative to this battery of tests. Although individually these tests are inexpensive, they are time consuming to perform. The use of a simple latex agglutination test provides a real-time 2 minute test for the confirmation to genus level of Listeria spp.

10 Listeria latex test Listeria can be used to obtain rapid, presumptive-positive results from selective media thus giving an early indication of a failure in the production process. Where standard methods require it, Listeria can be used to screen colonies before full biochemical identification and can replace traditional agglutinating antibody methods. Ordering Information: Code Description Size Storage F48 Listeria 50 tests 2-8 C Reagents/Kit: Reagents Product Code Detects Quantity/Size Listeria latex reagent Positive control F48a F48b 0.85% Isotonic saline F40 Latex particles coated with rabbit antibodies to Listeria spp. Positive control solution Sample preparation solution 0.5ml 5.0ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-092-EN revision 1.

11 Salmonella latex test Salmonella is a rapid latex slide agglutination test for the confirmatory identification of presumptive Salmonella colonies from selective agar plates. Salmonella test offers a rapid alternative to the traditional identification methods and detects Salmonella spp. including S. typhimurium and S. enteritidis. Rapid Results within 2 minutes. Eliminates the need for conventional screening tests that require 24 hours incubation Based on Salmonella Latex results laboratories can proceed directly to full biochemical identification High Specificity employs highly specific flagella antisera which reduces potential cross reactivity with non Salmonella Providing presumptive identification directly from primary isolation media Detects both motile and non motile Salmonella Rapid identification confirmed within 2 minutes Suitable for clinical and veterinary applications

12 Salmonella latex test Genus Salmonella The genus Salmonella consists of over 2,000 serotypes which can cause acute infectious gastroenteritis frequently associated with food poisoning. The rapid identification of Salmonella is important both for appropriate patient management and epidemiological investigations, minimising the delay associated with routine testing methods. Ordering Information: Code Description Size Storage M42CE Salmonella 50 tests 2-8 C Reagents/Kit: Reagents Product Code Contents Quantity/Size Salmonella latex reagent M42a Latex particles coated with rabbit antiserum against Salmonella antigens Positive control M42b Inactivated preparation of Salmonella antigen 0.5ml 0.85% Isotonic saline M40 Sample preparation solution 5.0ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-099-EN revision 1.

13 Strep latex test A rapid test for the confirmatory identification of Streptococci of Lancefield Groups A, B, C, D, F and G from culture plates. Most strains of Streptococci which have been isolated from human infections possess group specific antigens. Identification of the organisms includes extraction and characterisation of these antigens from organism grown in culture. The use of specific antisera combined with an enzyme extraction method provide a speedy and reliable typing system for Streptococci. Strep features Rapid grouping of Groups A, B, C, D, F, G Simple enzymic extraction Easy-to-read agglutination High specificity to group D Easy to use High sensitivity and specificity Results in one minute after extraction

14 Strep latex test Ordering Information: Code Description Size Storage M47CE Strep 50 tests 2-8 C Reagents/Kit: Reagents Product Code Contents Quantity/Size Reagent A M47a Contains rabbit Strep Group A antibody sensitised latex particles Reagent B M47b Contains rabbit Strep Group B antibody sensitised latex particles Reagent C M47c Contains rabbit Strep Group C antibody sensitised latex particles Reagent D M47d Contains rabbit Strep Group D antibody sensitised latex particles Reagent F M47f Contains rabbit Strep Group F antibody sensitised latex particles Reagent G M47g Contains rabbit Strep Group G antibody sensitised latex particles Positive controls M47p Positive control, contains inactivated polyvalent antigenic extracts to groups A, B, C, D, F and G 1.0ml Extraction enzyme M47x Lyophilised extraction enzyme 2x10ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-095-EN revision 1.

15 Staph latex test A rapid test for the identification and confirmation of Staphylococcus aureus colonies grown on selective solid media. Two of the traditional diagnostic tests for S. aureus, the tube and slide coagulase tests, only detect the activity of a characteristic coagulase enzyme. However, these tests can be difficult to interpret and are liable to suffer from false positive results associated with non-specific factors frequently found in plasma. Non-specific coagulation due to other bacterial enzymes may also occur. However, 99% of S. aureus isolates also produce detectable levels of protein A on their surface, a characteristic which can be used to differentiate S. aureus from other species of Staphylococci in which this protein is rare. Staph is a sensitive and specific latex agglutination for the identification of S. aureus which overcomes the problems associated with the traditional tests by detecting both coagulase and protein A, offering rapid and accurate identification of S. aureus in 2 minutes. Staph contains all ancillary reagents needed to complete the test and is suitable for clinical, food and environmental laboratories. Easy to use High sensitivity and specificity Results in 2 minutes

16 Staph latex test Staphylococcus spp. occur as commensal organisms on the mucous membranes and skin of humans. However, Staphylococcus aureus can be responsible for a wide range of pyogenic infections including superficial suppurative lesions, food poisoning and toxic shock, as well as a variety of other conditions. It is therefore one of the most important pathogens regularly encountered in the laboratory. Due to the frequency and importance of Staphylococcal infection a rapid and accurate diagnosis is needed for correct patient management, especially as resistance to numerous common antibiotics has been shown. Also available separately is the Staph latex control reagent (product code M43CCE), helping to reinforce the accuracy and reliability of the Staph rapid test. Ordering Information: Code Description Size Storage M43CE Staph latex test 100 tests 2-8 C M433CE Staph latex test 500 tests 2-8 C Reagents/Kit: Reagents Product Code Contents Quantity/Size Staph latex reagent M43a Latex particles coated with human fibrinogen and IgG 5ml 5x5ml for M433CE Staph positive control M43b Positive control 1ml If you need any further information on any of the above tests please contact Microgen Bioproducts Ltd. Microgen Bioproducts Ltd, 1 Admiralty Way, Camberley, Surrey GU15 3DT, United Kingdom T: +44 (0) F: +44 (0) E: sales.camberley@lab21.com GMM-097-EN revision 1.