Fall 2012 Office Phone: (714)

Transcription

1 Course Syllabus Derek Boyer Biology 210 Fall 2012 Office Phone: (714) Office: Bio Sc. 212A Hrs: T/Th 11:15-1:15pm Course Description: Biol 210 is a 5-unit college transferable course that introduces you to the microbial world a world of viruses, bacteria, molds, yeasts, protozoa, algae, and helminths. Emphasis will be placed on microbial structure, metabolic activities, microbial genetics, hostparasite relationships, host resistance, and the control of microbes. Lab activities will include isolation and cultivation of bacteria, studies of bacterial isolates, understanding bacterial population dynamics, control of microbial growth, mycology, parasitology, and virology. This course is recommended for biological science majors, pre-nursing, pre-medical, pre-dental, prepharmacy, and other life science majors. Required Textbook: Microbiology: An Introduction 11th Edition by Tortora, Funke, and Case Required Web Access to for online lecture quizzes and assignments Required Lab Manual: Laboratory Theory and Application, 3rd Ed. by Leboffe and Pierce Derek Boyer s webpage: has links to supplementary materials and a copy of this course syllabus. Student Learning Outcomes: 1. Demonstrate appropriate laboratory skills and techniques related to the isolation, staining, identification, assessment of metabolism, and control of microorganisms. 2. Evaluate the physical and chemical methods used in the control of microorganisms and apply this understanding to the prevention and control of infectious diseases when given a scenario. 3. Discuss and diagram the role of the immune system in maintaining homeostasis, challenging infections, and fighting cancer. Grading: Your course grade is based on a percent of accumulated points. These points are acquired by examinations, quizzes, lab assignments, and lab reports. The grading scale is: A = % (4) Lecture exams 125 pts. ea. = 500 B = 80-89% (TBD) Online lecture quizzes/assignments = 80 C = 70-79% (1) Written assignment 20 pts. = 20 D = 60-69% (2) Lab exams 80 pts. ea. = 160 F = Below 60% (1) Lab final 100 pts. = 100 (5) Lab quizzes 10 pts. ea. = 40 (1 drop) (3) Lab question sets 20/20/10 = 50 (1) Lab unknown project 30 pts. = 30 Lab participation/professionalism 20 pts. = 20 Total Possible Points = 1000 points

2 Lecture Policy Lecture has a total of 600 points that come from 4 exams, online quizzes and assignments, and 1 written assignment. Make-up exams: Only one make-up lecture exam will be allowed during the semester. Based on an excused absence you must contact me the day of the exam. Make-ups may be essay exams. Lab Policy Points for lab total 400 and come from 2 midterm exams, a final exam, 5 quizzes (lowest quiz/missed quiz will be dropped), question sets, an unknown project, and participation/professionalism. Late lab assignments may not be accepted or will be given a penalty depending on the time frame of the assignment. Note: You must take all lab practical exams during the time/day it is given, there is no way to make-up a lab practical exam on days they are not given. Please note that lab quizzes are given at the beginning of the period and take about 15 minutes. If you are late, you will not be given extra time to complete your quiz. There are no make-up quizzes. If you miss a quiz, this will count as your dropped quiz. Participation and Professionalism in lab are critical to this course; therefore, these two parts of your grade will count for 10 points each. Attendance (which is part of participation) will be taken at the beginning of each lab period. Only 1 absence will be allowed for the semester, after which 5 points will be deducted for two absences and 10 points for three absences. Your 10 points for professionalism will remain intact as long as the rules and safety guidelines of the lab are followed. General Policy Academic dishonesty on exams or quizzes will result in a score of zero for that exam or quiz and could be cause for an instructor drop (See the campus policy regarding academic dishonesty). Do not give or accept help during an exam! Attendance: Orange Coast College policy on attendance states: "...a student who has been absent for 3 consecutive class meetings in a given section is to be dropped by the instructor. Excellent attendance and class participation will be taken into consideration during final grading. Students in need of accommodations in the college learning environment: Tutoring for many different programs including Microbiology and English Language are available in the Student Success Center (located near the Blue Clock Tower). Any student who feels he/she may need an accommodation based on the impact of a learning disability or other disability should contact the Disabled Students Center (DSC) to schedule an appointment.

3 Week Date General Microbiology Lecture Topics Assigned Chapters 1 T Aug 28 Introduction 1 R Aug 30 History & Nomenclature 1 2 T Sep 4 Chemistry 2 R Sep 6 Chemistry 2 M Sep 10 ****Last Day to Withdraw to Avoid a W **** 3 T Sep 11 Prokaryotes & Eukaryotes 4 R Sep 13 Prokaryotes & Eukaryotes 4 4 T Sep 18 Prokaryotes & Eukaryotes 4 R Sep 20 LECTURE EXAM 1 1, 2, 4 5 T Sep 25 Microbial Metabolism 5 R Sep 27 Microbial Metabolism 5 6 T Oct 2 Microbial Metabolism 5 R Oct 4 Microbial Growth 6 7 T Oct 9 Microbial Growth 6 R Oct 11 Control and Antimicrobial Drugs 7, 20 8 T Oct 16 Control and Antimicrobial Drugs 7, 20 R Oct 18 Control and Antimicrobial Drugs 7, 20 9 T Oct 23 LECTURE EXAM 2 5, 6, 7, 20 R Oct 25 Microbial Genetics 8 10 T Oct 30 Microbial Genetics 8 R Nov 1 Fungi T Nov 6 Fungi 12 R Nov 8 Virology T Nov 13 Virology 13 R Nov 15 Virology 13 Sa Nov 17 ****Last Day to Drop**** 13 T Nov 20 LECTURE EXAM 3 8, 12, 13 R Nov 22 Holiday Thanksgiving, No Lecture 14 T Nov 27 Microbial Pathogenesis 15 R Nov 29 Microbial Pathogenesis T Dec 4 Immunology 16, 17 R Dec 6 Immunology 16, T Dec 11 Immunology / Vaccines 17, 18 R Dec 13 LECTURE EXAM

4 WK Date Projected Lab Topics 1 M/T Aug 27/28 Orientation; Lab safety; Microscopy 3-1 View slides of colored threads, blood smears, and the letter e Set up environmental plates (Incubate at 37 C) 2-1 W/R Aug 29/30 Wet mounts of cheek cells; Aseptic technique, Simple stain 1-3, 3-5 Check and discuss environmental plates (Observe Bacteria) 2-1; 2-2 *Continue growing environmental plates at room temp. 2 M/T Sept 3/4 Holiday - Labor Day, No Lab W/R Sept 5/6 Isolation of bacteria (Part I): Streak plate #1 1-4 Negative Stain; Gram stain 3-6; M/T Sept 10/11 Check Streak Plate #1 and set up Streak Plate #2 1-4 Yeast and filamentous fungi: Tape mounts; Slide cultures (demo) 12-1; 12-2 Check and discuss environmental plates (Observe Fungi) 2-1; 2-2 W/R Sept 12/13 Quiz 1; Isolation of bacteria (Part II): Standard plate count 6-1 Endospore stain; Check Streak plate # Carbohydrate Catabolism/Fermentation; Respiration 4 M/T Sept 17/18 OF-glucose; PR-broth; Capsule stain; Acid-fast stain 5-2; 5-3; 3-9; 3-8 Read results for Standard plate count W/R Sept 19/20 IMViC (Indole/MRVP/Citrate) 5-20, 5-4, 5-8 Starch hydrolysis; ONPG 5-12; 5-18 Read results for OF-glucose, PR-broth; Preparation of media (demo) M/T Sept 24/25 Quiz 2; Read results for IMViC, Starch hydrolysis, and ONPG W/R Sept 26/27 Lab Practical Exam #1 Protein Catabolism 6 M/T Oct 1/2 Decarboxylase (LDC, ODC); Phenylalanine; Urease; Casease 5-10; 5-11; 5-13; 5-14 TSI (H 2 S only) 5-21 (and 5-20) W/R Oct 3/4 Gelatinase; Motility Agar 5-15; 5-28 Read results for Phenylalanine, Urease, Casease, and TSI (H 2 S only) Respiration 7 M/T Oct 8/9 Anaerobic respiration: Anaerobic jar, Nitrate reduction 2-8, 5-7 Aerobic respiration: Catalase, Oxidase 5-5, 5-6 Read results for Decarboxylase, Gelatinase, Motility Agar W/R Oct 10/11 Set up Temperature exp.; ph demo; Osmotic pressure demo 2-9; 2-10; 2-11 Thioglycollate broth demo 2-7 Read results for the Anaerobic jar and Nitrate reduction *Remember antiseptics/disinfectants for next class Control of Microbial Growth 8 M/T Oct 15/16 Quiz 3; UV light; Disinfectants and antiseptics 2-13, 2-14 Antimicrobial susceptibility test (Kirby-Bauer method) 7-3 Read results from the Temperature experiment W/R Oct 17/18 View Protozoan slides 12-3 Read UV, Disinfectant/antiseptic, and Kirby-Bauer plates **Remember water samples for next class Lab Exercises

5 WK Date Projected Lab Topics Microbes in Food/Water & Bacteria of the GI-Tract 9 M/T Oct 22/23 Set up Water Analysis: Membrane filter technique, LTB tubes 8-12; 8-13 Parasite video; View Protozoan slides (continued) 12-3 Unknown project begins Streak onto TSA & MacConkey s agar 7-7, 4-5 W/R Oct 24/25 Water Analysis (continued): BGLB tubes 8-13 Epidemiology experiment; Finish protozoan slides 7-6; 12-3 Read results for Membrane filters and LTB tubes Work on unknown projects Gram staining and IMViC 10 M/T Oct 29/30 Quiz 4; Read results for Epidemiology and BGLB tubes 7-6; 8-13 Review W/R Oct 31/1 Lab Practical Exam #2 Bacteria of the Skin, Respiratory Tract, and Mouth 11 M/T Nov 5/6 Nasal swab on MSA; Throat swab on blood agar 4-4; 5-25 Set up CAMP tests with Bacitracin test (A-disk), 5-26; 5-24 Optochin (O-disk); Bile Esculin 5-24; 4-3 W/R Nov 7/8 Nasal swab isolate: Coagulase test, Gram stain 5-27; 3-7 Throat swab isolate: Gram stain and identification handout Read CAMP tests; View hemolysis patterns demo 5-26 Bacteria of the mouth (discussion only) 12 M/T Nov 12/13 Holiday Veteran s Day, No Lab W/R Nov 14/15 Virus video; Set up Plaque Assay 6-5 Work on unknown projects Lab Exercises 13 M/T Nov 19/20 Blood smear differentials; Blood typing 11-1; 11-5 Read results for Plaque Assay W/R Nov 21/22 Holiday - Thanksgiving, No Lab Bacteria of the Urogenital Tract 14 M/T Nov 26/27 Urinalysis: Bactercult and HurBi biplate 6-2 Triple Sugar Iron (TSI) Agar 5-21 View Helminth Slides 12-4 W/R Nov 28/29 Quiz 5; Read results for the Bacturcult, BurBi biplate, and TSI View Helminth Slides (continued) M/T Dec 3/4 Review; Finish/work on unknown projects W/R Dec 5/6 Final Lab Practical Exam (comprehensive) 16 M/T Dec 10/11 Clean up the lab; Finish all lecture material W/R Dec 12/13 No Lab; Lecture Final Exam

6 SAFETY ISSUES IN THE BIOLOGY 210 MICROBIOLOGY LABORATORY 1. Do not come to lab late, new procedures (which often involve safety) are discussed at the beginning of lab. 2. Wear a lab coat or smock at all times in the lab. Store your coat/smock on the coat rack or in an assigned drawer. 3. Be aware of Bunsen burner flames. Turn off burners when not in use. Secure long hair to prevent accidental exposure to open flames. Report burns and other injuries to your lab instructor immediately. 4. Use the eye wash station immediately, should any reagent accidentally get into your eyes. 5. Do not eat, drink, or store, food/drinks in the lab. 6. Place contaminated items in autoclavable containers for disposal. Do not mix glass with plastic items. 7. Use the biohazard bag to dispose of biological waste DO NOT use the trash basket to dispose of biological waste. 8. Remove old cultures from the refrigerator or incubator and place them in the biohazard bag for disposal. Remove labels from glassware when disposing. Do not remove cultures from the lab or place them in your lab drawer. 9. Report accidental spills of cultures to your instructor immediately. Do not attempt to pick up broken glass yourself. 10. Try to avoid bringing your hands to your mouth while in lab. 11. Wash your hands with soap and water before leaving lab. 12 Cover cuts on exposed skin with a sterile bandage. 13. Disinfect your desktop at the beginning and end of lab. 14. Inform your lab instructor if you are pregnant or if you are immunosuppressed as some lab exercises may pose unique problems in such cases. 15. Wear goggles when using reagents and when near ultraviolet lights. 16. Use proper aseptic technique when making culture transfers.