Product Datasheet. Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] NB7539. Unit Size: 1 ml. Store at 4C. Do not freeze.

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1 Product Datasheet Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] NB7539 Unit Size: 1 ml Store at 4C. Do not freeze. Reviews: 1 Publications: 12 Protocols, Publications, Related Products, Reviews, Research Tools and Images at: Updated 10/7/2017 v.20.1 Earn rewards for product reviews and publications. Submit a publication at Submit a review at

2 NB7539 Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] Product Information Unit Size Concentration Storage Clonality 1 ml 1.0 mg/ml Store at 4C. Do not freeze. Polyclonal Preservative 0.1% Pro-Clean 400 Isotype Conjugate Purity Buffer IgG HRP Immunogen affinity purified Phosphate Buffered Saline (PBS) containing 0.2%BSA Page 1 of 4 v.20.1 Updated 10/7/2017 Product Description Host Species Specificity/Sensitivity Immunogen Goat Mouse By immunoelectrophoresis and ELISA this reacts specifically with mouse IgG and with light chains common to other mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Mouse IgG-heavy and light chain Product Application Details Applications Western Blot, ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin Recommended Dilutions Western Blot 1:5000-1:50000, ELISA 1: : , Immunohistochemistry 1:40-1:400, Immunocytochemistry/Immunofluorescence 1:50-1:500, Immunohistochemistry-Paraffin 1:40-1:400

3 Images Western Blot: Goat anti-mouse, Human IgG (H+L) Secondary Antibody [HRP] [NB7539] - Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] Page 2 of 4 v.20.1 Updated 10/7/2017 Immunohistochemistry-Paraffin: Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - Immunohistochemistry-Paraffin: Goat anti- Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse antip53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-mouse IgG-heavy and light chain Antibody HRP Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB Western Blot: Goat anti-mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - Goat anti-mouse IgG Antibody [HRP] [NB7539] - This secondary antibody used for detection of beta-actin in human pancreatic cancer cell lines. Image from verified customer review.

4 Publications Kalainayakan SP, Ghosh P, Dey S et al. Cyclopamine tartrate, a modulator of hedgehog signaling and mitochondrial respiration, effectively arrests lung tumor growth and progression Sci Rep Feb :00AM [PMID: ] (IHC-P, Mouse) Lu J, Shen X, Sun X et al. Increased recruitment of endogenous stem cells and chondrogenic differentiation by a composite scaffold containing bone marrow homing peptide for cartilage regeneration. Theranostics. Oct :00AM [PMID: ] (IHC, Mouse) Wang X, Hybiske K, Stephens RS Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells. Pathog Dis 2018 Jan 30 [PMID: ] (WB, Mouse) Wang X, Hybiske K, Stephens RS Direct visualization of the expression and localization of chlamydial effector proteins within infected host cells. Pathog Dis Jan 30 [PMID: ] (WB, Mouse) Perlenfein TJ, Murphy RM. A mechanistic model to predict effects of cathepsin B and cystatin C on b-amyloid aggregation and degradation. J. Biol. Chem Oct 18 [PMID: ] (WB, Mouse) Ebneter JA, Heusser SD, Schraner EM et al. Cyst-Wall-Protein-1 is fundamental for Golgi-like organelle neogenesis and cyst-wall biosynthesis in Giardia lamblia. Nat Commun. Dec :00AM [PMID: ] (WB, Parasite) Moghimi E, Solomon JA, Gianforcaro A, Hamadeh MJ. Dietary Vitamin D3 Restriction Exacerbates Disease Pathophysiology in the Spinal Cord of the G93A Mouse Model of Amyotrophic Lateral Sclerosis. PLoS One 2015 [PMID: ] (WB, Mouse) Meerson A, Traurig M, Ossowski V et al. Human adipose microrna-221 is upregulated in obesity and affects fat metabolism downstream of leptin and TNF-alpha. Diabetologia 2013 Jun 12 [PMID: ] (WB, Mouse) Giessrigl B, Krieger S, Rosner M et al. Hsp90 stabilizes Cdc25A and counteracts heat shock-mediated Cdc25A degradation and cell-cycle attenuation in pancreatic carcinoma cells. Hum Mol Genet 2012 Nov [PMID: ] Lin YT, Ding JY, Li MY et al. YAP regulates neuronal differentiation through Sonic hedgehog signaling pathway. Exp Cell Res 2012 Sep [PMID: ] Kim HJ, Lim SJ, Kwag HL et al. The choice of resin-bound ligand affects the structure and immunogenicity of columnpurified human papillomavirus type 16 virus-like particles. PLoS One 2012 [PMID: ] Soenawan E, Srivastava I, Gupta S et al. Maintenance of long-term immunological memory by low avidity IgMsecreting cells in bone marrow after mucosal immunizations with cholera toxin adjuvant. Vaccine 2004 Mar [PMID: ] More publications at Page 3 of 4 v.20.1 Updated 10/7/2017

5 Novus Biologicals USA E. Briarwood Avenue Centennial, CO USA Phone: Toll Free: Fax: Novus Biologicals Canada 461 North Service Road West, Unit B37 Oakville, ON L6M 2V5 Canada Phone: Toll Free: Fax: Novus Biologicals Europe 19 Barton Lane Abingdon Science Park Abingdon, OX14 3NB, United Kingdom Phone: (44) (0) Free Phone: Fax: (44) (0) General Contact Information Technical Support: Orders: General: Limitations This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt. For more information on our 100% guarantee, please visit Earn gift cards/discounts by submitting a review: Earn gift cards/discounts by submitting a publication using this product: