Supporting Information
|
|
- Juliana Shepherd
- 5 years ago
- Views:
Transcription
1 Supporting Information Real-time Quantitative PCR Measurements of Fecal Indicator Bacteria and Human-associated Source Tracking Markers in a Texas River following Hurricane Harvey Vikram Kapoor*, Indrani Gupta, A.B.M. Tanvir Pasha, and Duc Phan Department of Civil and Environmental Engineering, University of Texas at San Antonio, San Antonio, TX 78249, USA *Corresponding author vikram.kapoor@utsa.edu S-1
2 Description of sampling sites. Sites G1 and G2 were located on the segment of Guadalupe River north of Victoria, TX. Sites G3 and G4 were located upstream and downstream from a wastewater treatment plant (WWTP), respectively. Sites G5 and G6 were located on the Guadalupe River closest to the Texas coast, before and after the confluence of San Antonio River, respectively. Sites SA1 and SA2 located on the San Antonio River were used as control sites since they were in close proximity to Guadalupe River and were not severely impacted due to flooding. Sites G4 and G5 were classified as flooded catchment of the Guadalupe River, while sites G1, G2, G3 and G6 were located in the non-flooded segment of Guadalupe River. Sites SA1 and SA2 were located in the non-flooded segments of the San Antonio River. DNA extraction. DNA was extracted from filter samples using the DNeasy PowerLyzer PowerSoil Kit (Qiagen, Germantown, MD) according to the manufacturer s protocol. Extraction controls with autoclaved distilled water were used during filtration to monitor for potential extraneous DNA contamination. The concentration and purity of DNA was determined using NanoDrop OneC Spectrophotometer (Thermo Scientific, Wilmington, DE). Total DNA concentrations ranged from 2 to 30 ng/µl and DNA extract quality was confirmed by absorbance measurements at 260/280 nm, yielding ratios of DNA extracts were stored at 20 C for no longer than 3 months prior to qpcr analysis. qpcr assays. The occurrence and relative abundance of E. coli, enterococci, and humanspecific Bacteroidales (BacHum and HF183) was measured using previously described TaqMan qpcr assays (Table S1) and DNA extracts (2 µl) as the templates. Reaction mixtures (25 µl) contained 1 SsoAdvanced Universal Probes Supermix (Bio-Rad, Hercules, CA), 0.2 µm (final concentration) of each primer, and a 6-FAM (6-carboxyfluorescein)-labeled hydrolysis probe. All reaction mixtures were prepared in duplicate and qpcr assays were performed on CFX96 S-2
3 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA). The qpcr data were analyzed using Bio-Rad's CFX Manager Software (version 3.1). Three independent standard curves were generated for each qpcr assay by using serially diluted plasmid standards purchased from Integrated DNA Technologies (IDT, Skokie, IL) containing the sequences for each of the targeted genes. Each standard curve was generated from at least six 10-fold plasmid dilutions in triplicate. The percent amplification efficiencies were calculated by the instrument manufacturer's instructions (Bio-Rad). No-template controls (two per plate) were used to check for cross contamination, and 10-fold dilutions of each DNA extract were used to test for PCR inhibition. PCR products were also visualized using E-Gel 2% with SYBR Safe (Invitrogen, Green Island, NY) to confirm the size of amplification products. S-3
4 Figure S1. Guadalupe River at Victoria TX crested at 10 ft above flood stage on Aug 31, (Reproduced from ref. 1). S-4
5 Figure S2. NASA's IMERG rainfall analysis for Tropical Storm Harvey covers the period from Aug. 21 through Aug. 28, IMERG shows rainfall totals to 10 inches (red shading) from the coast near San Antonio Bay to in and around the Victoria area. Sites G4, G5 and G6 were located in the heavy rainfall area (solid line square) while sites G1, G2, G3 and SA1, SA2 were loacted in light rainfall areas (dashed line squares). Although site G6 was located in the heavy rainfall area, it was categorized as non-flooded site since it was diluted by the non-flooded San Antonio River. (Reproduced from ref. 2). S-5
6 Figure S3. Correlation between human-specific Bacteroidales and fecal indicators using qpcr (n = 32). The linear regression lines between human-specific Bacteroidales and conventional indicators are represented as a solid line for Entero1 and dashed line for E. coli. S-6
7 Table S1. Primers and probes used in this study. Assay Primer and probe sequences (5-3 ) Reference E. coli (EC23S857) General Enterococcus (Entero1) Human-specific Bacteroidales (BacHum) Human-specific Bacteroidales (HF183) F: GGTAGAGCACTGTTTtGGCA* R: TGTCTCCCGTGATAACtTTCTC* P: 6FAM-TCATCCCGACTTACCAACCCG-TAMRA ECST748F: AGAAATTCCAAACGAACTTG ENC854R: CAGTGCTCTACCTCCATCATT GPL813TQ: 6FAM-TGGTTCTCTCCGAAATAGCTTTAGGGCTA- TAMRA BacHum-160f: TGAGTTCACATGTCCGCATGA BacHum-241r: CGTTACCCCGCCTACTATCTAATG BacHum-193p: 6FAM-TCCGGTAGACGATGGGGATGCGTT- TAMRA HF183-1: ATCATGAGTTCACATGTCCG BthetR1: CGTAGGAGTTTGGACCGTGT BthetP1: 6FAM-CTGAGAGGAAGGTCCCCCACATTGGA- TAMRA Chern et al. 3 Ludwig & Schleifer 4 Kildare et al. 5 Haugland et al. 6 6FAM, 6-carboxyfluorescein, fluorescence reporter dye; TAMRA, 6-carboxytetramethylrhodamine, fluorescence quencher dye; *Lower case denotes deliberately mismatched base. S-7
8 References 1. U.S. Geological Survey. USGS Current Conditions for the Nation. (accessed October 1, 2017). 2. National Aeronautics and Space Administration. NASA Calculates Tropical Storm Harvey's Flooding Rainfall. (accessed October 1, 2017). 3. Chern, E. C.; Siefring, S.; Paar, J.; Doolittle, M.; Haugland, R. A. Comparison of quantitative PCR assays for Escherichia coli targeting ribosomal RNA and single copy genes. Lett. Appl. Microbiol. 2011, 52, Ludwig, W.; Schleifer, K. H. How quantitative is quantitative PCR with respect to cell counts?. Syst. Appl. Microbiol. 2000, 23, Kildare, B. J.; Leutenegger, C. M.; McSwain, B. S.; Bambic, D. G.; Rajal, V. B.; Wuertz, S. 16S rrna-based assays for quantitative detection of universal, human-, cow-, and dog-specific fecal Bacteroidales: a Bayesian approach. Water Res. 2007, 41, Haugland, R. A.; Varma, M.; Sivaganesan, M.; Kelty, C.; Peed, L.; Shanks, O. C. Evaluation of genetic markers from the 16S rrna gene V2 region for use in quantitative detection of selected Bacteroidales species and human fecal waste by qpcr. Syst. Appl. Microbiol. 2010, 33, S-8
Supplemental Materials. DNA preparation. Dehalogenimonas lykanthroporepellens strain BL-DC-9 T (=ATCC
Supplemental Materials DNA preparation. Dehalogenimonas lykanthroporepellens strain BL-DC-9 T (=ATCC BAA-1523 = JCM 15061) was grown in defined basal medium amended with 0.5 mm 1,1,2- trichloroethane (1,1,2-TCA)
More informationExecutive Summary. clinical supply services
clinical supply services case study Development and NDA-level validation of quantitative polymerase chain reaction (qpcr) procedure for detection and quantification of residual E.coli genomic DNA Executive
More informationHope College MST Methods Summary
Hope College MST Methods Summary Sample collection: Stream samples were obtained in the field by hand using sterile (autoclaved) 1-liter polypropylene storage bottles. Collected samples were placed on
More informationTechnical Review. Real time PCR
Technical Review Real time PCR Normal PCR: Analyze with agarose gel Normal PCR vs Real time PCR Real-time PCR, also known as quantitative PCR (qpcr) or kinetic PCR Key feature: Used to amplify and simultaneously
More informationQPCR-S100 (100 rxns) Store -20. Functional analysis RealHelix qpcr Kit was evaluated by real-time PCR using the 10-fold serial-diluted human
RealHelix TM qpcr Kit [Intercalator type] CERTIFICATE OF ANALYSIS (1603-V01R03) Kit contents RealHelix TM qpcr Kit [Intercalator type] Cat. No. QPCR-S100 (100 rxns) QPCR-S500 (500 rxns) 2x qpcr PreMix
More informationGenetic Sequencing Methodologies to Assess Human Contributions of Fecal Coliforms to a Freshwater Receiving Stream Introduction Sample Collection
Genetic Sequencing Methodologies to Assess Human Contributions of Fecal Coliforms to a Freshwater Receiving Stream D. A Graves1, D. E. Chestnut1, E. B. Rabon1, W. J. Jones2, J. G. Moore3, C. Johnston3
More information4985 SW 74th Court, Miami, FL USA Tel: (1) , Fax: (1) ,
4985 SW 74th Court, Miami, FL 33155 USA Tel: (1) 786-220-0379, Fax: (1) 786-513-2733, Email: info@sourcemolecular.com Preliminary Interpretation of Human Fecal Pollution ID TM Results Detection and quantification
More informationMarteilia refringens detection and typing by Real time Polymerase Chain Reaction
Edition n 1 European Union Reference Laboratory for molluscs diseases Marteilia refringens detection and typing by Real time Polymerase Chain Reaction CONTENTS 1. Scope... 2 2. References... 2 3. Equipment
More informationCat. # RR391A. For Research Use. Probe qpcr Mix. Product Manual. v201610da
Cat. # RR391A For Research Use Probe qpcr Mix Product Manual Table of Contents I. Introduction... 3 II. Principle... 3 III. Components... 5 IV. Materials Required but not Provided... 5 V. Storage... 5
More information3color RT HS-PCR Mix SYBR Ready-to-use mix for real-time Hot Start PCR with SYBR Green. Dedicated for white reaction tubes and plates.
3color RT HS-PCR Mix SYBR Ready-to-use mix for real-time Hot Start PCR with SYBR Green. Dedicated for white reaction tubes and plates. version 0217 250 reactions in 20 μl Cat. # 2000-250S 2500 reactions
More informationSUPPORTING INFORMATION. A cleavage-responsive stem-loop hairpin for assaying guide RNA activity
SUPPORTING INFORMATION A cleavage-responsive stem-loop hairpin for assaying guide RNA activity Tara R. deboer 1, Noreen Wauford 1, Jing-Yi Chung, Miguel Salvador Torres Perez, and Niren Murthy* University
More informationDetection and quantification of porcine adenoviruses by real-time PCR
Page 1 of 6 EU FP VII PROJECT VITAL STANDARD OPERATING CREATED: REVISED: APPROVED: David Rodríguez Lázaro: 18-2-2010 FERA: 28-03-2010 Wim Van der Poel: 31-03-2010 Page 2 of 6 WARNING All samples and controls
More informationPremix Ex Taq (Probe qpcr)
For Research Use Premix Ex Taq (Probe qpcr) Product Manual Table of Contents I. Description... 3 II. Principle... 4 III. Components... 5 IV. Materials Required but not Provided... 5 V. Storage... 5 VI.
More informationDetection of Biological Threat Agents by Real-Time PCR - Comparison of Assay
Detection of Biological Threat Agents by Real-Time PCR - Comparison of Assay Performance on the Idaho Technology, Inc. R.A.P.I.D., the Roche LightCycler, and the Cepheid Smart Cycler Supplemental Data
More informationPrimePCR Assay Validation Report
Gene Information Gene Name keratin 78 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID KRT78 Human This gene is a member of the type II keratin gene family
More informationRealHelix TM qrt-pcr Kit [Intercalator type]
RealHelix TM qrt-pcr Kit [Intercalator type] CERTIFICATE OF ANALYSIS (1603-V01R03) Kit contents RealHelix TM qrt-pcr Kit [Intercalator type] Cat. No. QRT-S100 (100 rxns) QRT-S500 (500 rxns) qrt-pcr Enzyme
More informationApplications and Uses. (adapted from Roche RealTime PCR Application Manual)
What Can You Do With qpcr? Applications and Uses (adapted from Roche RealTime PCR Application Manual) What is qpcr? Real time PCR also known as quantitative PCR (qpcr) measures PCR amplification as it
More informationHuman bocavirus. genesig Standard Kit. Viral protein (VP) gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Human bocavirus Viral protein (VP) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human bocavirus Human Bocavirus is a genus of the
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID mcf.2 transforming sequence-like Mcf2l Mouse Description Not Available C130040G20Rik,
More informationDetection and quantification of hepatitis E virus by real-time reverse transcriptase PCR
Page 1 of 6 EU FP VII PROJECT VITAL STANDARD OPERATING Detection and quantification of hepatitis E virus by real-time reverse transcriptase PCR CREATED: REVISED: APPROVED: David Rodríguez Lázaro: 18-2-2010
More informationPRODUCT INFORMATION Thermo Scientific Luminaris Probe Low ROX qpcr Master Mix #K0944 For 5000 rxns Lot Expiry Date Store at -20 C in the dark CERTIFICATE OF ANALYSIS The absence of endo-, exodeoxyribonucleases
More informationSupporting Text. Methods
Supporting Text Methods PCR Amplification and Sequencing of amoa. Each reaction mixture contained 12.5 µl of MasterAmp PCR premix F (Epicentre Technologies, Madison, WI), 0.5 µm of each primer (Qiagen,
More informationRelative Quantification: Data Management & Analysis Settings
Relative Quantification: Data Management & Analysis Settings Ray Meng, Ph.D. International Field Applications Specialist Gene Expression Division Bio-Rad Laboratories Gene Expression AMPLIFICATION Assay
More informationDevelopment and Application of a Rapid, User-Friendly and Inexpensive. Method to Detect Dehalococcoides sp. Reductive Dehalogenase Genes from
1 Supplementary Section 2 3 Applied Microbiology and Biotechnology 4 5 6 7 Development and Application of a Rapid, User-Friendly and Inexpensive Method to Detect Dehalococcoides sp. Reductive Dehalogenase
More informationmirna Purification from Plant Tissues: Corn, Soybean and Arabidopsis
mirna Purification from Plant Tissues: Corn, Soybean and Arabidopsis A Maxwell RSC mirna Tissue Kit Application Note Materials Required: Maxwell RSC mirna Tissue Kit (Cat.# AS1480) QuantiFluor RNA System
More informationValidation of qpcr Rapid Bacterial Quantification through Viable E. coli cell count in the Saginaw Bay Watershed
Validation of qpcr Rapid Bacterial Quantification through Viable E. coli cell count in the Saginaw Bay Watershed TYLER LEFEVRE ADVISOR: DR. TAMI SIVY 1 Overview Introduction to fecal coliforms Current
More informationhpsc Genetic Analysis Kit
qpcr analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and ips cells Catalog #07550 60 Reactions Product Description hpsc Genetic Analysis Kit contains nine primer-probe
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID Glyceraldehyde-3-phosphate dehydrogenase Gapdh Rat This gene encodes a member of
More informationDNA Quantitation NBS Molecular Training Class June 28 30, 2011
DNA Quantitation NBS Molecular Training Class June 28 30, 2011 Suzanne Cordovado, PhD Molecular Quality Improvement Program, CDC National Center for Environmental Health Centers for Disease Control and
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID Peptidyl-glycine alpha-amidating monooxygenase Peptidylglycine alpha-hydroxylating
More informationPrimePCR Assay Validation Report
Gene Information Gene Name collagen, type IV, alpha 1 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID COL4A1 Human This gene encodes the major type IV alpha
More informationIsolation of High-Purity Total Cellular RNA from Muscle Tissues Using the Agilent Total RNA Isolation Mini Kit Application
Isolation of High-Purity Total Cellular RNA from Muscle Tissues Using the Total RNA Isolation Mini Kit Application Genomics Author Christopher Rizzo Technologies, Inc. 2850 Centerville Road Wilmington,
More informationM. tuberculosis_mpb64/is61 10 genesig Standard Kit
TM Primerdesign Ltd M. tuberculosis_mpb64/is61 10 genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to M.tuberculosis_MPB64/IS6110 2 Specificity MAX MIN The Primerdesign
More informationFunctional Genomics Research Stream. Research Meeting: June 19, 2012 SYBR Green qpcr, Research Update
Functional Genomics Research Stream Research Meeting: June 19, 2012 SYBR Green qpcr, Research Update Updates Alternate Lab Meeting Fridays 11:30-1:00 WEL 4.224 Welcome to attend either one Lab Log thanks
More informationCopy number standard curve. for absolute quantification by real-time PCR
Copy number standard curve for absolute quantification by real-time PCR Kit contents Positive control template for standard curve (RED) Template preparation buffer (YELLOW) RNase/DNase free water (WHITE)
More informationQUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 2007)
QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 007) Please contact Center for Plant Genomics (CPG) facility manager Hailing Jin (hljin@iastate.edu) regarding questions or corrections.
More informationPhosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for resuspending the SingleShot RNA control template
Catalog # Description 172-5085 SingleShot SYBR Green Kit, 100 x 50 µl reactions For research purposes only. Introduction The SingleShot SYBR Green Kit prepares genomic DNA (gdna) free RNA directly from
More informationGeneCopoeia TM. All-in-One qpcr Mix For universal quantitative real-time PCR. User Manual
GeneCopoeia TM Expressway to Discovery All-in-One qpcr Mix For universal quantitative real-time PCR Cat. No. AOPR-0200 (200 qpcr reactions) Cat. No. AOPR-0600 (600 qpcr reactions) Cat. No. AOPR-1000 (1000
More informationGuidelines for Developing Robust and Reliable PCR Assays
Guidelines for Developing Robust and Reliable PCR Assays Leta Steffen, PhD Applications Scientist Promega Corporation Outline 1) PCR reaction components What is in the reaction? How does it affect assay
More informationAmoyDx JAK2 Mutation Detection Kit
AmoyDx JAK2 Mutation Detection Kit Detection of V617F mutation in the JAK2 oncogene Instruction for Use For Research Use Only Instruction Version: B2.6 Revision Date: October 2013 Store at -20±5 Xiamen,
More informationMycobacterium Tuberculosis
TM Primerdesign Ltd Mycobacterium Tuberculosis rep13e12 genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis
More informationAntibiotic resistance VanA
TM Primerdesign Ltd Antibiotic resistance VanA Vancomycin resistance protein A (vana) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Antibiotic resistance
More informationFastFire qpcr PreMix (Probe)
FastFire qpcr PreMix (Probe) For fast, quantitative, specific real-time PCR using sequence-specific probe www.tiangen.com/en FP130820 Kit Contents FastFire qpcr PreMix (Probe) Contents 2 FastFire qpcr
More informationAmoyDx TM RRM1 Gene Expression Analysis Kit
AmoyDx TM RRM1 Gene Expression Analysis Kit Instructions For Use Instructions Version: B2.1 Date of Revision: April 2012 Store at -20±2 o C -1/5- Background RRM1 is one of the targets of Gemcitabine, and
More informationQuantiNova proven compatibility with various real-time cyclers in gene expression analysis
QuantiNova proven compatibility with various real-time cyclers in gene expression analysis QuantiNova SYBR Green and QuantiNova Probe PCR Kits were used on various real-time cyclers, and compared with
More informationReal Time Quantitative PCR Assay Validation, Optimization and Troubleshooting
Real Time Quantitative PCR Assay Validation, Optimization and Troubleshooting Dr Steffen Muller Field Applications Scientist Stratagene Europe Overview The Importance of Controls Validation and Optimization
More informationQuantitative Real time PCR. Only for teaching purposes - not for reproduction or sale
Quantitative Real time PCR PCR reaction conventional versus real time PCR real time PCR principles threshold cycle C T efficiency relative quantification reference genes primers detection chemistry GLP
More informationA Recommended Procedure for Real-Time Quantitative TaqMan PCR for Roundup Ready Canola RT73 Monsanto Biotechnology Regulatory Sciences
Page 1 of 7 Overview Purpose & Scope This procedure describes an event-specific real-time TaqMan PCR method for determination of the relative content of Roundup Ready canola RT73 (hereafter referred to
More informationCryptococcus neoformans
TM Primerdesign Ltd Cryptococcus neoformans Hypothetical protein genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Cryptococcus neoformans Cryptococcus neoformans
More informationEscherichia coli_o104: H4
PCRmax Ltd TM qpcr test Escherichia coli_o104: H4 150 tests For general laboratory and research use only 1 Introduction to Escherichia coli_o104:h4 2 Specificity MAX MIN The PCR Max qpcr Kit for Escherichia
More informationqpcr Quantitative PCR or Real-time PCR Gives a measurement of PCR product at end of each cycle real time
qpcr qpcr Quantitative PCR or Real-time PCR Gives a measurement of PCR product at end of each cycle real time Differs from endpoint PCR gel on last cycle Used to determines relative amount of template
More informationSuperReal PreMix Plus (SYBR Green)
SuperReal PreMix Plus (SYBR Green) For fast, quantitative, real-time PCR using SYBR Green www.tiangen.com/en QP120627 SuperReal PreMix Plus (SYBR Green) Cat. no. FP205 Kit Contents Contents 2 SuperReal
More informationNucleic Acid Research Group Real-Time PCR Survey
Nucleic Acid Research Group Real-Time PCR Survey Association of Biomolecular Resource Facilities (ABRF) INTRODUCTION: This survey is designed to determine the current status of real-time PCR technology
More informationAdenovirus Type F and G
TM Primerdesign Ltd Adenovirus Type F and G Hexon gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Adenovirus Type F and G Adenoviruses are medium-sized
More informationDetection and quantification of bovine polyomavirus by real-time PCR
Page 1 of 5 EU FP VII PROJECT VITAL STANDARD OPERATING CREATED: REVISED: APPROVED: David Rodríguez Lázaro: 18-02-2010 FERA: 28-03-2010 Wim Van der Poel: 31-03-2010 Page 2 of 5 WARNING All samples and controls
More informationSaccharomyces cerevisiae
TM Primerdesign Ltd Saccharomyces cerevisiae Actin (ACT1) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Saccharomyces cerevisiae Saccharomyces cerevisiae
More informationPrimePCR Assay Validation Report
Gene Information Gene Name keratin associated protein 9-2 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID KRTAP9-2 Human This protein is a member of the keratin-associated
More informationInvestigation of qpcr Inhibition Occurrence across Geographically Distributed Recreational Waters
Investigation of qpcr Inhibition Occurrence across Geographically Distributed Recreational Waters Tamara Anan eva US EPA Office of Water ORISE Research Fellow City of Racine, WI Health Department Laboratory
More informationHumqPCR-realtime JEV. Japanese encephalitisvirus - Real Time. Instrument Compatibility in: 50 / 100 /150 tests (Ready to use kit)
Japanese encephalitisvirus - Real Time 50 / 100 /150 tests (Ready to use kit) Real Time Kit is a screening assay for the detection of Japanese encephalitisvirus. Principles of the test: The Japanese encephalitisvirus
More informationMycobacterium Tuberculosis
TM Primerdesign Ltd Mycobacterium Tuberculosis rep13e12 genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin)
More informationLactobacillus 16S Ribosomal RNA. genesig Advanced Kit. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Lactobacillus 16S Ribosomal RNA genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Lactobacillus The Lactobacillus genus is a group of Gram-positive,
More informationPrimePCR Assay Validation Report
Gene Information Gene Name keratin 3 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID KRT3 Human The protein encoded by this gene is a member of the keratin
More informationSOP: SYBR Green-based real-time RT-PCR
SOP: SYBR Green-based real-time RT-PCR By Richard Yu Research fellow Centre for Marine Environmental Research and Innovative Technology (MERIT) Department of Biology and Chemistry City University of Hong
More informationPrimePCR Assay Validation Report
Gene Information Gene Name integrin, alpha 1 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID ITGA1 Human This gene encodes the alpha 1 subunit of integrin
More informationEstimation of Enterococcus qpcr target sequence recoveries from EPA NEEAR study calibrator samples 2014 UNC Water Micro Conference
Estimation of Enterococcus qpcr target sequence recoveries from EPA NEEAR study calibrator samples 2014 UNC Water Micro Conference Office of Research and Development May 2014. Notice: Although this work
More informationM. tuberculosis_mpb64/is611. genesig Advanced Kit. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd M. tuberculosis_mpb64/is611 0 genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to M.tuberculosis_MPB64/IS6110 2 Specificity MAX MIN The Primerdesign
More informationStaphylococcus epidermidis
TM Primerdesign Ltd Staphylococcus epidermidis superoxide dismutase (soda) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Staphylococcus epidermidis
More informationHuman Herpes Virus 7 genomes
TM Primerdesign Ltd Human Herpes Virus 7 genomes Glycoprotein H gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Herpes Virus 7 genomes Human Herpesvirus
More informationEpstein Barr Virus (Human Herpes virus 4)
TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to
More informationPurpose: To sequence 16S microbial DNA isolated from animal fecal matter and perform compositional analysis of bacterial communities.
Purpose: To sequence 16S microbial DNA isolated from animal fecal matter and perform compositional analysis of bacterial communities. Reference: This protocol was adapted from: A custom and streamlined
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID protein phosphatase, Mg2+/Mn2+ dependent, 1A PPM1A Human The protein encoded by
More informationUser Manual. VisiBlue qpcr mix colorant. Version 1.3 September 2012 For use in quantitative real-time PCR
User Manual VisiBlue qpcr mix colorant Version 1.3 September 2012 For use in quantitative real-time PCR VisiBlue qpcr mix colorant Table of contents Background 4 Contents 4 Storage 4 Fluorescence data
More informationAccurately and reproducibly quantify as little as 20 fg of bacterial DNA using real-time PCR.
INSTRUCTION MANUAL Femto Bacterial DNA Quantification Kit Catalog No. E2006 Highlights Accurately and reproducibly quantify as little as 20 fg of bacterial DNA using real-time PCR. High specificity and
More informationUser Manual. NGS Library qpcr Quantification Kit (Illumina compatible)
NGS Library qpcr Quantification Kit (Illumina compatible) User Manual 384 Oyster Point Blvd, Suite 15 South San Francisco, CA 94080 Phone: 1 (888) MCLAB-88 Fax: 1 (650) 872-0253 www.mclab.com Contents
More informationCat. # RR430S RR430A. For Research Use. SYBR Fast qpcr Mix. Product Manual. v201610da
For Research Use SYBR Fast qpcr Mix Product Manual Table of Contents I. Description... 3 II. Principle... 4 III. Components... 5 IV. Storage... 5 V. Features... 6 VI. Precautions... 6 VII. Protocol...
More informationEvent COT102 Cotton. Real-time, Event-specific Polymerase Chain Reaction Method
Event COT102 Cotton Real-time, Event-specific Polymerase Chain Reaction Method Syngenta Crop Protection, LLC 3054 East Cornwallis Road PO Box 12257 Research Triangle Park, NC 27709-2257, USA For technical
More informationQuantitative Real time PCR. Only for teaching purposes - not for reproduction or sale
Quantitative Real time PCR PCR reaction conventional versus real time PCR real time PCR principles threshold cycle C T efficiency relative quantification reference genes primers detection chemistry GLP
More informationPQL-S200 (200 rxns) Store -20. using the 10-fold serial-diluted human genomic DNA and a set of human gene-specific primer
CERTIFICATE OF ANALYSIS (1603-V01R02) RealHelix TM Premier q Kit [SYBR Green with low ROX] Kit contents RealHelix TM Premier q Kit [SYBR Green with low ROX] Cat. No. PQL-S200 (200 rxns) PQL-S500 (500 rxns)
More informationOnly for teaching purposes - not for reproduction or sale
PCR reaction conventional versus real time PCR real time PCR principles threshold cycle C T efficiency relative quantification reference genes primers detection chemistry GLP in real time PCR Relative
More informationMycobacterium (all species)
TM Primerdesign Ltd Mycobacterium (all species) 16S rrna gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Mycobacterium (all species) Mycobacteria are
More informationPrimePCR Assay Validation Report
Gene Information Gene Name Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID myeloperoxidase MPO Human Myeloperoxidase (MPO) is a heme protein synthesized during
More informationDBS DNA Extraction, Validation & Quantitation
DBS DNA Extraction, Validation & Quantitation Suzanne Cordovado, PhD Molecular Quality Improvement Program NBS Molecular Training Class July 8, 2013 National Center for Environmental Health Centers for
More informationEvaluation of rapid QPCR method for enterococci with correlative assessment for molecular markers for sewage contamination in selected environmental
Evaluation of rapid QPCR method for enterococci with correlative assessment for molecular markers for sewage contamination in selected environmental water samples from Hawaii. Problem and Research Objectives
More informationHelicobacter pylori. genesig Standard Kit. RNA polymerase beta-subunit (rpob) gene. 150 tests. Primerdesign Ltd
TM Primerdesign Ltd Helicobacter pylori RNA polymerase beta-subunit (rpob) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Helicobacter pylori Helicobacter
More informationAntibiotic resistance OXA -48 Carbapenem-hydrolyzing β- lactamase (blaoxa-48) gene. genesig Advanced Kit. 150 tests.
TM Primerdesign Ltd Antibiotic resistance OXA -48 Carbapenem-hydrolyzing β- lactamase (blaoxa-48) gene genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Antibiotic
More informationEvent-specific Method for the Quantification of Soybean CV127 Using Real-time PCR. Validation Report
Event-specific Method for the Quantification of Soybean CV127 Using Real-time PCR Validation Report 20 September 2011 Joint Research Centre Institute for Health and Consumer Protection Molecular Biology
More informationUsing Today s Data to Close the Beach Today. Quantitative Polymerase Chain Reaction (QPCR) rapid beach closings tool.
1 Using Today s Data to Close the Beach Today. Quantitative Polymerase Chain Reaction (QPCR) rapid beach closings tool. Richard A. Haugland USEPA, Office of Research and Development, National Exposure
More informationQuantitative PCR for Genetic Markers of Human Fecal Pollution
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2009, p. 5507 5513 Vol. 75, No. 17 0099-2240/09/$08.00 0 doi:10.1128/aem.00305-09 Quantitative PCR for Genetic Markers of Human Fecal Pollution Orin C. Shanks,
More informationQuantitative PCR for Genetic Markers of Human Fecal Pollution
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Sept. 2009, p. 5507 5513 Vol. 75, No. 17 0099-2240/09/$08.00 0 doi:10.1128/aem.00305-09 Quantitative PCR for Genetic Markers of Human Fecal Pollution Orin C. Shanks,
More informationPhosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for resuspending the SingleShot RNA control template
Catalog # Description 172-5090 SingleShot Probes Kit, 100 x 50 µl reactions For research purposes only. Introduction The SingleShot Probes Kit prepares genomic DNA (gdna) free RNA directly from cell culture
More informationEpstein Barr Virus (Human Herpes virus 4)
PCRmax Ltd TM qpcr test Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene 150 tests For general laboratory and research use only 1 Introduction to Epstein Barr Virus
More informationSYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk
Cat. # RR820L For Research Use SYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk Product Manual The long-term storage temperature of this product has been changed to -20 since Lot. AK9104. See section IV.
More informationPrimePCR Assay Validation Report
Gene Information Gene Name minichromosome maintenance complex component 8 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID MCM8 Human The protein encoded by
More informationMycoplasma bovis. genesig Standard Kit. DNA gyrase subunit B (gyrb) gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Mycoplasma bovis DNA gyrase subunit B (gyrb) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Mycoplasma bovis Mycoplasmas are small,
More informationEvent-specific Method for the Quantification of maize MON by Real-time PCR. Validated Method
EUROPEAN COMMISSION JOINT RESEARCH CENTRE Health, Consumers & Reference Materials Food & Feed Compliance Unit Event-specific Method for the Quantification of maize MON 87403 by Real-time PCR Validated
More informationAssay Design Considerations, Optimization and Validation
Assay Design Considerations, Optimization and Validation Ray Meng, Ph.D. International Field Applications Specialist Gene Expression Division Bio-Rad Laboratories, Inc. Assay Design Considerations Experiment
More informationPrimePCR Assay Validation Report
Gene Information Gene Name SRY (sex determining region Y)-box 6 Gene Symbol Organism Gene Summary Gene Aliases RefSeq Accession No. UniGene ID Ensembl Gene ID SOX6 Human This gene encodes a member of the
More informationFMV 35S promoter (GMO) Advanced Kit. FMV 35S promoter. 150 tests. For general laboratory and research use only
FMV 35S promoter (GMO) FMV 35S promoter 150 tests Advanced Kit For general laboratory and research use only Introduction to FMV 35S promoter (GMO) The kit provides a method for detecting gene insertion
More information