Falcon Cell Culture Multi-Flask An easier, more productive way to culture cells
|
|
- Baldric Owens
- 6 years ago
- Views:
Transcription
1 Falcon Cell Culture Multi-Flask An easier, more productive way to culture cells Falcon Cell Culture Multi-Flasks enable you to grow more cells faster and easier, thereby making your cell culture workflow more productive. The tissue culture-treated Falcon Multi-Flasks are available in 3- or 5-layer formats that provide 525 cm 2 or 875 cm 2 cell growth surface area. They can be used with a wide range of liquid volumes (up to 50 ml/layer). Features Even distribution of media across all layers for homogeneous cell populations. Ability to mix in the Falcon Multi-Flask saves time and reduces risk of contamination. Flexible design lets you pour or aspirate/recover cells using a pipet. Consistent surface treatments for predictable scale-up. Manufactured in compliance to cgmp standards. Designed to Fit Your Protocol Simplify your scale-up. Falcon Multi-Flasks, offer the same footprint, the same reagent volumes, and the same cell seeding densities as 175 cm² flasks. Plus, you ll be using the same proven surface treatment as all other Falcon flasks. Improves Your Cell Culture Productivity Falcon Multi-Flasks deliver a thoughtful design that simplifies your workflow. You can eliminate multiple steps and reduce the risk of contamination. Pipet access allows you to aspirate media to exchange with fresh media and recover cells without pouring. The mixing port allows rapid mixing within the Falcon Multi-Flask to create your cell suspension, transfect your cells, or add other reagents directly to the flask. The port also provides for a uniform distribution of media and cells to facilitate homogeneous cell growth on all layers. More Consistent Cell Growth Falcon Multi-Flasks even distribution of media, proven vacuum-gas tissue culture surface treatment, and effective gas exchange all combine to provide an optimal cell culture environment. The result is higher cell yield and a more homogeneous cell population. Visit for more information. FALCON
2 PREDICTABLE SCALE-UP Cell Number x Cell Number x Corning T175 Flask (1-layer) Falcon Multi-Flask (3-layer) CELL CULTURE VESSEL Falcon Multi-Flask (5-layer) 0 BHK-21 LnCap Hep-G2 EcoPack CELL LINE Expected Yield Actual Yield T-175 Falcon Multi-Flask (3-layer) Falcon Multi-Flask (5-layer) Three and five times more BHK-21 cells were grown and recovered from 3- and 5-layer Falcon Multi-Flasks than T-175 flasks (left). Cell yields per cm 2 were equivalent in 3- and 5-layer Falcon Multi-Flasks and T-175 flasks for BHK-21, LnCap, Hep-G2, and EcoPack cells (right). CONSISTENT CELL PHYSIOLOGY CHO-M1 cells were grown in a T-175 and a 3-layer Falcon Multi-Flask for 72 hours. Cells were harvested and re-seeded on 96-well PDL plates for GPCR Calcium mobilization assay. Cells were challenged with M1 agonist, Carbachol (300 µm) and responses were recorded 1 minute following compound addition. Data is expressed as fold-increase in calcium flux compared to control cells challenged with assay buffer (no drug). No significant difference in agonist response was observed between cells harvested from either vessel type. Data shown is representative of three independent experiments. Calcium Mobilization Fold Increase Over Control (mean + SD) T-175 CELL CULTURE VESSEL Falcon 3-layer SIMPLIFIED WORKFLOW Pipet access eliminates the need for pouring. It also facilitates the use of a wide range of liquid volumes (up to 50 ml/layer), efficient recovery of valuable cells and reagents, and minimizes the risk of contamination. Mixing port eliminates mixing outside the vessel, saving you time and effort. This unique in-vessel mixing allows for rapid and uniform distribution of cells and reagents. Compatibility with similar volumes of reagent usage and cell seeding densities as T-175 flasks makes scaling up a familiar and simple process.
3 CONSISTENT CELL GROWTH This figure illustrates homogeneous cell growth between layers of Falcon Multi-Flasks. BHK-21 cells grown to >80% confluence in 3-layer Falcon Multi-Flasks and control T-175 flasks were fixed and stained with crystal violet. Control flasks and individual layers of the multi-flask vessels were cut and scanned. Top Middle Bottom Corning T-175 (control) IDEAL FOR PLURIPOTENT STEM CELLS Human Mesenchymal Stem Cells were cultured in MSCGM medium (Lonza) and seeded at 4,000 to 5,000 cells/cm 2 in a T-175 Falcon Flask and a 3-layer Falcon Multi-Flask. The cells were cultured until they reached 80% confluence (~6 days). Cells were harvested, counted, and cumulative population doublings of hmscs from each vessel type were calculated over five consecutive passages. Immunophenotypic properties of hmscs grown in the Falcon Multi-Flask as determined by flow cytometric analysis (FACS) confirmed that hmscs were negative for surface markers CD14, CD34, CD45, CD79a, and HLA-DR and positive for expression of surface markers CD73, CD90, and CD105. Respective isotype controls for each marker antibody are shown in grey. COMPATIBLE WITH YOUR CELL LINES Diverse cell lines and primary cultures (with and without serum) can be grown and efficiently recovered from Falcon Multi-Flasks. Expected yield was determined using average cell yield from control T-175 flasks multiplied by three and five times for the 3- and 5-layer Falcon Multi-Flasks, respectively.
4 Falcon Cell Culture Multi-Flask An easier, more productive way to culture cells PRODUCT SPECIFICATIONS WORKING VOLUME RANGE >5 ml per layer for dissociating <50 ml per layer for cell expansion MOLDED-IN GRADUATIONS 0 to 50 ml per layer in 10 ml increments GRADUATION ACCURACY 10% CAP VENT MEMBRANE 0.2 μm hydrophobic membrane CELL GROWTH SURFACE Tissue culture-treated, optically clear ORDER INFORMATION Falcon Cell Culture Multi-Flask Description Qty/Pack Qty/Case Cat. No. 3-LAYER TISSUE CULTURE-TREATED, 525 CM LAYER TISSUE CULTURE-TREATED, 875 CM To place an order in Europe, contact Customer Service at: tel: , fax: ; CSEurope@Corning.com For technical assistance, contact Technical Support at: cctech@corning.com ACCESSORIES Description Qty/Pack Qty/Case Cat. No. Falcon Pipets ASPIRATING PIPET 2 ML INDIVIDUALLY WRAPPED ML INDIVIDUALLY WRAPPED PAPER-PLASTIC ML INDIVIDUALLY WRAPPED PLASTIC-PLASTIC Falcon Tubes 50 ML POLYPROPYLENE CONICAL TUBE ML POLYPROPYLENE CONICAL TUBE ML POLYPROPYLENE CONICAL TUBE U.S. Orders Contact your authorized distributor to place your order. Corning acquired the Discovery Labware Business including the Falcon brand. For information, visit For a listing of trademarks, visit us at All other trademarks are property of their respective owners. Corning Incorporated, One Riverfront Plaza, Corning, NY Corning Incorporated Life Sciences Corning BV Fogostraat LJ Amsterdam The Netherlands Phone: Fax: cceurnl@corning.com , 2013 Corning Incorporated Printed in Europe 02/13 CLS-DL-CC-024 A4
5 Falcon Multi-Flask Frequently Asked Questions FALCON Why does media spill over to the other layers when moving the Falcon Multi-Flask in and out of the incubator? Transport the flask according to the user guide (see step 8) using the partition position and keeping fluid away from the mix port. Do not carry Falcon Multi-Flasks in a horizontal position as this may cause spillover of media between layers. Why does media distribution become uneven when I place the Falcon Multi-Flask in the incubator? Fluid may migrate from one layer to the other. To prevent this from occurring, one should: Ensure the incubator shelf is level. Position flasks across shelf without blocking the vented neck region. Ensure weight is distributed evenly throughout the shelf and the shelf can withstand the weight of flasks with media. Use the opposite corner of the mix port to pivot the flask and lay it horizontally. Ensure media does not flow back into the mix port or over the shelves into the neck region. I followed the user guide and set the Falcon Multi-Flask into the incubator. A few hours later, I see uneven media distribution by layer. What do I do? Repeat re-equilibration and partitioning steps (user guide steps 7 and 8). Ensure the work surface is flat while equilibrating and partitioning fluid in the Falcon Multi-Flask. Inspect the flask at least 2 hours after seeding. Re-equilibrate and partition again, if necessary. Minimize vibration on, or near, the surface where Falcon Multi-Flasks are housed. What volume of media should I use? Begin with the same media volume and cell seeding density on a per unit surface area basis as your existing device. The surface area of the flasks are 525 cm 2 for 3-layer and 875 cm 2 for 5-layer flasks. The maximum amount of media used should not exceed 50 ml per layer. What volume of dissociation reagent should I use? We recommend 5 ml per layer of Falcon Multi-Flask. Can I use the same volume of reagents as T-175 flasks? No, the amount of media per unit area should be the same not the actual total volume. If you currently use a T-175 flask, simply multiply the volume by 3 or 5 times depending on the Falcon Multi-Flask format used.
6 Why do I observe excessive frothing or bubbling of media and can this affect my cell culture? Over mixing can result in excessive frothing or bubbling of media, which can lead to fluid migration from one layer to another. To avoid this, follow user guide step 5. While the flask is in the mixing position, allow the media to drain only from the layer on top; immediately tilt the flask in the opposite direction; and repeat. Ensure the flask is brought back to the mix position (user guide step 4) before equilibration of media. Improper mixing or pouring of media into the flask can also create foaming or bubbles. The correct way to add media is to follow step 1 in the user guide and pour or pipet gently along the slope of the flask lid. How can the flasks be stacked in an incubator? Ensure the flasks lock into position using the stacking ribs. Position the stacks of flasks evenly in the incubator to prevent the shelves from bowing. Allow adequate room for air circulation through the vented cap of the flasks. When stacking the Falcon Multi-Flask on incubator shelves that are above shoulder height, ensure the media distribution by layer remains equal. The use a step-stool can help facilitate flask placement. What is the best way to add cells to the vessel? Add the desired volume of growth media to the flask and keep it in an upright position. Add cell suspension stock (at least 10% final volume) directly into the growth medium using a 10 ml pipet. Is pre-wetting of the flask or pre-warming of the media in the flask required? Neither is required, nor are they recommended unless you are applying a coating to the flask growth surface. The 5-layer Falcon Multi-Flask will not fit under my microscope. What do you recommend? With some inverted microscopes, the condenser can be removed or moved to accommodate the 5-layer flasks. If this is not possible, a control flask (T-175) can be run side-by-side to provide an indication of cell morphology and confluence. Why is cell growth patchy when I view the Falcon Multi-Flask under the microscope? Uneven cell/media distribution most likely occurs from inadequate mixing. Refer to steps 4 and 5 of the user guide for the proper procedure. Don't use a clumpy cell suspension stock. Ensure cells are adequately triturated to generate a single cell suspension. Why am I getting media in the neck of the flask? Media should not enter the neck of the flask if the Falcon Multi-Flask is used properly. Check to ensure the flask is not upside down. The logo should face up when the flask is laid horizontally for incubation. Over-rotation when mixing cells in the vessel can result in media getting into the neck area of the flask. To avoid this, please follow steps 4 to 6 in the user guide. Why are my cells not attaching? If your cells normally attach to a single layer flask, then they will attach to the surface of the Falcon Multi-Flask. Ensure the flask is laid in the correct position with the logo facing up. In this position, cells will adhere to the tissue culture surface on the base of each layer.
7 Can I pour my cell suspension into the flask? Yes, however we recommend that to avoid foaming of media, use a 50 or 100 ml pipet (Cat. Nos and ). Insert the pipet past the logo and stream liquid along the top slope of the flask into the first layer. Can I pour my cells and reagents out of the flask? Yes, you can pour or use a pipet to recover the cells during harvesting. If cells clump, mechanically dissociate the cells by titrating the suspension. How can I maximize the recovery of cells and reagents? To minimize residual volumes, pour as per step 13 in the user guide. Allow liquid to pool on the mix port side prior to pouring with the logo down. For maximal recovery of cells and reagents, follow steps 11 and 12 in the user guide. First, aspirate by positioning the pipet tip towards the mix port region. Then, pool liquid into the top layer and tilt the flask to partition liquid away from mix port. For high density cell cultures, additional rinses are recommended. Visually inspect the base layer to determine how many rinses are required. Why am I not getting the expected cell yield? You may not be recovering all the cells. Additional rinses may be required, as noted above. You may have uneven distribution of cells and media. See the user guide for proper mixing, equalization and partitioning procedures. You should avoid lengthy delays between cell seeding and incubation so that cells do not settle to the bottom of the vessel.
Falcon Multi-Flask FEATURES:
Falcon Multi-Flask Long to-do list? Falcon Multi-Flasks can help. These convenient 3- and 5-layer flasks provide greater surface area and cell yield than single-layer T-175 flasks, but with the same footprint
More informationExpansion of Human Mesenchymal Stem Cells Using Corning HYPER Technology Cell Culture Vessels
Expansion of Human Mesenchymal Stem Cells Using Corning HYPER Technology Cell Culture Vessels SnAPPshots A brief technical report from the Corning Development Group Ana Maria P. Pardo and Mark E. Rothenberg,
More informationProtocol for Small-Scale Microcarrier Culture
Protocol for Small-Scale Microcarrier Culture Hillex II Microcarriers This document provides a general protocol for initiation of small-scale mammalian cell spinner cultures. This procedure is based upon
More informationApplication Note 493
Corning PureCoat ECM Mimetic Cultureware Collagen I Peptide: Novel Synthetic, Animal-free Surface for Culture of Human Keratinocytes Kerry Thompson, Jeff Partridge, Elizabeth Abraham, Paula Flaherty, Susan
More informationExpansion of Human Mesenchymal Stem Cells Using Corning HYPER Technology Cell Culture Vessels
Expansion of Human Mesenchymal Stem Cells Using Corning HYPER Technology Cell Culture Vessels SnAPPshots A brief technical report from the Corning Development Group Introduction Ana Maria P. Pardo and
More informationCellartis ipsc Single-Cell Cloning DEF-CS Culture Media Kit
Takara Bio Europe AB Cellartis ipsc Single-Cell Cloning DEF-CS Culture Media Kit Cat. No. Y30021 (050517) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe 20, SE-413 46 Göteborg, Sweden
More informationCorning PureCoat Cultureware. Where life thrives on the surface
Corning PureCoat Cultureware Where life thrives on the surface Corning PureCoat Surfaces Optimized for Basic Research and Drug Discovery Applications Advantages of Corning PureCoat Amine and Carboxyl Surfaces
More informationGuidelines for WTC Derived AICS hipsc Lines Scale Up & Banking (upto 80 vials)
Version 1.2 2017 Allen Institute for Cell Science Guidelines for WTC Derived AICS hipsc Lines Scale Up & Banking (upto 80 vials) Note: The following protocol is for banking ~60-80 vials of cells (1 million/vial)
More informationLiquid. This product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.
PRIME-XV MSC Expansion SFM PRIME-XV MSC Expansion SFM is a serum-free complete medium optimized for the maintenance and expansion of purified human MSCs. This product does not contain antibiotics. Catalog
More informationSTEMdiff Mesenchymal Progenitor Kit
Defined culture kit for derivation and expansion of mesenchymal progenitor cells Catalog #05240 1 Kit Product Description STEMdiff Mesenchymal Progenitor Kit is a defined culture kit consisting of animal
More informationCell Migration, Chemotaxis and Invasion Assay Using Staining
Cell Migration, Chemotaxis and Invasion Assay Using Staining Protocol Hillary Sherman and Mark Rothenberg Corning Life Sciences 836 North St. Bldg. 300, Suite 3401 Tewksbury, MA 01876 Introduction Cell
More informationAll quality control test results are reported on a lot specific Certificate of Analysis which is available at or upon request.
PRIME-XV NPC Expansion XSFM PRIME-XV NPC Expansion XSFM is a xeno-and serum-free medium optimized for the cultivation and expansion of neural progenitor cells (NPC) that are able to maintain their ability
More informationCorning Life Science Equipment
PROMOTIONAL OFFER Corning Life Science Equipment Liquid Handling Centrifugation Agitation Constant Temperature Valid May 1 st 2018 to December 15 th 2018 Promotion Liquid Handling Equipment Corning Stripettor
More informationCore Cell Culture Product Training
Core Cell Culture Product Training Cell Culture What is Cell Culture? Complex process of growing cells under controlled conditions in vitro (outside of the body) Way to simulate cell growth in vivo (in
More informationBD Ratiometric Calcium Assay Kit
BD Technical Data Sheet BD Ratiometric Calcium Assay Kit Product Information Catalog Number: 644243 Components: Ratiometric Calcium Indicator, 1 vial, lyophilized 10X Signal Enhancer, 10 ml Calcium Assay
More informationCellartis Human ips Cell Lines User Manual
Takara Bio Europe AB Cellartis Human ips Cell Lines User Manual Cat. Nos. Y00185, Y00225, Y00265, Y00275, Y00285, Y00305, Y00315, Y00325 (061215) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens
More informationTransIT -LT1 Transfection Reagent
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2300 INTRODUCTION TransIT -LT1 Transfection Reagent is a broad spectrum reagent that provides high efficiency plasmid
More informationRatiometric Calcium Assay Kit
BD Technical Data Sheet Ratiometric Calcium Assay Kit Product Information Catalog Number: 644244 Components: Ratiometric Calcium Indicator, 10 vials, lyophilized 10X Signal Enhancer, 100 ml Description
More informationTransIT Transfection Reagent
INTRODUCTION TransIT -2020 is a broad spectrum transfection reagent that provides superior transfection of plasmid DNA into mammalian cells. TransIT-2020 is suitable for both transient and stable transfection
More informationCalcium Assay Kit. Technical Data Sheet. Product Information. Description. Storage. Materials not included
BD Technical Data Sheet Calcium Assay Kit Product Information Catalog Number: 640176 Size Reagents for 10 plates Components: Calcium Indicator, 1 vial, lyophilized 10X Signal Enhancer, 10 ml 1X Calcium
More informationTransIT -Insect Transfection Reagent
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/6100 INTRODUCTION TransIT -Insect Transfection Reagent is a novel transfection formulation specifically developed for
More informationCellartis MSC Xeno-Free Culture Medium
Cat. # Y50200 For Research Use Cellartis MSC Xeno-Free Culture Medium Product Manual Table of Contents I. Description... 3 II. Contents... 3 III. Storage... 3 IV. Precautions... 3 V. Materials Required
More informationTransIT -293 Transfection Reagent
TransIT -293 Transfection Reagent Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2700 INTRODUCTION TransIT -293 Transfection Reagent is specifically optimized to provide
More informationTransfection Reagent Protocol for MIR 2110, 2114, 2115, 2116
TransIT-siQUEST Transfection Reagent INTRODUCTION TransIT-siQUEST is a broad spectrum sirna transfection reagent that enables high efficiency sirna delivery and knockdown of target gene expression in many
More informationTransIT -CHO Transfection Kit
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2170 INTRODUCTION TransIT -CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency
More informationTransIT -LT1 Transfection Reagent
Protocol for Product Nos. MIR 2300, 2304, 2305, 2306 i n t r o d u c t i o n The easy to use TransIT-LT1 (Low Toxicity) Transfection Reagent provides superior transfection efficiency, cell viability, and
More informationCulture and freezing methods for AICS cell lines
Allen Institute for Cell Science Culture and freezing methods for AICS cell lines Required Reagent List: Complete mtesr1 culture media, referred to in this protocol as simply mtesr1 : 400 ml basal media
More informationCloneMedia-CHO Semi-solid media for the growth of CHO-S Colonies
Protocol CloneMedia-CHO Semi-solid media for the growth of CHO-S Colonies Control #: 03PCT1010.A0 Effective Date: 01-Jul-11 ECO #: 4002 Content Page Introduction 3 Protocol 4 1. Media Preparation 4 2.
More informationCorning BioCoat Matrigel Matrix 6-well Plates for Embryonic Stem (ES) Cell Culture. Catalog Number Guidelines for Use
Corning BioCoat Matrigel Matrix 6-well Plates for Embryonic Stem (ES) Cell Culture Catalog Number 354671 Guidelines for Use Discovery Labware, Inc., Two Oak Park, Bedford, MA 01730, Tel: 1.978.442.2200
More informationCell Culture Solutions. Tough problems require innovative Solutions from Corning
Cell Culture Solutions Tough problems require innovative Solutions from Corning Corning s Innovative Cell Culture Cell Culture Solutions Problems isolating tumor or adult stem cells? Problems with cell
More informationMeasuring Neuronal Activity:
icell GlutaNeurons Application Protocol Measuring Neuronal Activity: Maestro Multielectrode Array Introduction icell GlutaNeurons, human glutamatergic cortical neurons derived from induced pluripotent
More informationTransIT-TKO Transfection Reagent
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2150 INTRODUCTION TransIT-TKO is a broad spectrum sirna transfection reagent that enables high efficiency sirna delivery
More informationTransfection Reagent SPECIFICATIONS MATERIALS. For Research Use Only. Materials Supplied. Materials required, but not supplied
TransIT-siQUEST Transfection Reagent Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2110 INTRODUCTION TransIT-siQUEST is a broad spectrum sirna transfection reagent
More informationAll quality control test results are reported on a lot specific Certificate of Analysis which is available at or upon request.
PRIME-XV MSC Expansion XSFM PRIME-XV MSC Expansion XSFM is a serum-free and xeno-free complete medium optimized for the maintenance and expansion of purified human MSCs. This product does not contain antibiotics.
More informationTransIT -LT1 Transfection Reagent
INTRODUCTION TransIT -LT1 Transfection Reagent is a broad spectrum reagent that provides high efficiency plasmid DNA delivery in many mammalian cell types including primary cells. TransIT-LT1 is a low
More informationTransIT -Insect Transfection Reagent
INTRODUCTION TransIT -Insect Transfection Reagent is a novel transfection formulation specifically developed for high-performance transfection of plasmid DNA into insect cells such as: Sf9, High Five (BTI-TN-5B1-4),
More informationT ECHNICAL MANUAL. Culture of Human Mesenchymal Stem Cells Using MesenCult -XF Medium
T ECHNICAL MANUAL Culture of Human Mesenchymal Stem Cells Using MesenCult -XF Medium i Table of Contents 1.0 Materials... 1 1.1 MesenCult -XF Medium and Required Products... 1 1.2 Additional Required
More informationAll quality control test results are reported on a lot specific Certificate of Analysis which is available at or upon request.
PRIME-XV MSC Expansion XSFM Catalog # Product Size 91149 PRIME-XV MSC Expansion XSFM 250 ml and 1 L liquid Intended Use This product is for research use or further manufacturing use only. This product
More informationTransIT -BrCa Transfection Reagent
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/5500 INTRODUCTION TransIT -BrCa Transfection Reagent is specifically optimized to provide exceptional transfection efficiency
More informationTransIT -Prostate Transfection Kit
INTRODUCTION TransIT -Prostate Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in Prostate cell types. Generally, prostate cell types have been
More informationTransfection Reagent INTRODUCTION SPECIFICATIONS MATERIALS. For Research Use Only. Materials Supplied. Materials required, but not supplied
TransIT-Neural Transfection Reagent INTRODUCTION TransIT-Neural Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in various neural cell types.
More informationCloneMedia and XP Media CHO Growth A Reagents Table 0-1: Available Reagents
CloneMedia and XP Media CHO Growth A Reagents Table 0-1: Available Reagents Item Quantity Part Number CloneMedia CHO Growth A with L-Gln, 90 ml 1 bottle K8810 CloneMedia CHO Growth A with L-Gln, 90 ml
More informationTransIT -Keratinocyte Transfection Reagent
TransIT -Keratinocyte Transfection Reagent Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2800 INTRODUCTION TransIT -Keratinocyte Transfection Reagent is specifically
More informationTransIT -293 Transfection Reagent
INTRODUCTION TransIT -293 Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HEK 293 and cell types of associated lineage. TransIT-293 provides
More informationApplication Note. Introduction. Kerry Thompson, Jeff Partridge, Paula Flaherty, Susan Qian, and Deepa Saxena
Page 1 BD PureCoat ECM Mimetic Cultureware Fibronectin Peptide: Novel Synthetic, Animalfree Surface for Culture of Human Bone Marrow-derived Mesenchymal Stem Cells Kerry Thompson, Jeff Partridge, Paula
More informationTransIT Transfection Reagent
INTRODUCTION TransIT -2020 Transfection Reagent is a high-performance, animal-origin free, broad spectrum reagent that provides exceptional transfection of plasmid DNA into mammalian cells. TransIT-2020
More informationTransIT -BrCa Transfection Reagent
INTRODUCTION TransIT -BrCa Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in breast cancer and related cell types including: MCF-7, MDA-MB-231
More informationProtocol for BelloCell-500AP Operation ver.1.0
Protocol for BelloCell-500AP Operation ver.1.0 Tabel of Content Page I. Culture Initiative and Inoculation 2-4 II. Start the Circulation System 5 III. Sampling of Culture Medium 6 IV. Cell Count by Crystal
More informationTransIT-X2 Dynamic Delivery System
INTRODUCTION TransIT-X2 Dynamic Delivery System is an advanced, non-liposomal polymeric system that enables high efficiency transfection of many cell types, including primary cells. TransIT-X2 can be used
More informationNeural Stem Cells (ipsc from Blood Cells; Male)
Applied StemCell, Inc. (866) 497-4180 www.appliedstemcell.com Datasheet Neural Stem Cells (ipsc from Blood Cells; Male) Product Information Catalog Number ASE-9234 (Male) Description Applied StemCell's
More informationCardiomyocyte Differentiation Methods
Version 1.0 2018 Allen Institute for Cell Science Cardiomyocyte Differentiation Methods The methods described below were adapted from the small molecule protocol described in Lian et al. 1, and have been
More informationTHE DELIVERY EXPERTS. INTERFERin in vitro sirna transfection reagent PROTOCOL
THE DELIVERY EXPERTS INTERFERin in vitro sirna transfection reagent PROTOCOL DESCRIPTION INTERFERin is a powerful sirna transfection reagent that ensures efficient gene silencing and reproducible transfection
More informationINTERFERin sirna transfection reagent
INTERFERin in vitro sirna Transfection Protocol Company Information... 2 Product Information... 3 1. Standard sirna transfection of adherent cells... 4 (forward transfection)... 4 1.1 Cell seeding... 5
More informationicell DopaNeurons Kit
icell DopaNeurons Application Protocol Measuring Synchronous Neuronal Activity on the Maestro Multielectrode Array Introduction icell DopaNeurons, human induced pluripotent stem cell (ipsc)-derived neurons,
More informationBeginning-to-end Solutions for Cell Culture
At Corning, cells are in our culture. In our continuous efforts to improve efficiencies and develop new tools and technologies for life science researchers, we have scientists working in Corning R&D labs
More informationSTEMdiff Hematopoietic Kit
For differentiation of human ES or ips cells into hematopoietic progenitor cells Catalog #05310 1 Kit Product Description Kit includes a defined, serum-free basal medium and supplements for the feeder-free
More informationTransIT -mrna Transfection Kit
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2225 INTRODUCTION TransIT -mrna Transfection Kit is designed to transfect RNA into a broad range of cell types with
More informationAdaptation of hpscs from Different Culture Systems to Essential 8 Medium or Essential 8 Flex Medium
Adaptation of hpscs from Different Culture Systems to Essential 8 Medium or Essential 8 Flex Medium Publication Part Number MAN0013768 Revision A.0 Introduction This protocol describes the transition of
More informationCellartis DEF-CS Xeno-Free Culture Media User Manual
Takara Bio Europe AB Cellartis DEF-CS Xeno-Free Culture Media User Manual Cat. Nos. Y30045 (030917) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe 20, SE-413 46 Göteborg, Sweden Europe
More informationTransIT -CHO Transfection Kit
INTRODUCTION TransIT -CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in CHO cells and cell types of associated lineage. TransIT-CHO Transfection
More informationAll quality control test results are reported on a lot specific Certificate of Analysis which is available at or upon request.
PRIME-XV Neural Basal Medium PRIME-XV Neural Basal Medium is a chemically-defined basal medium optimized for the culture and maintenance of neuronal cells when supplemented with PRIME-XV IS21 Supplement
More informationTransIT -CHO Transfection Kit
INTRODUCTION TransIT -CHO Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in CHO cells and cell types of associated lineage. TransIT-CHO Transfection
More informationWELCOME TO THE FUTURE OF TRANSIENT EXPRESSION. Smart start guide for the ExpiCHO system
WELCOME TO THE FUTURE OF TRANSIENT EXPRESSION Smart start guide for the ExpiCHO system 2 Contents Smart start tips 6 Shake grid 7 Frequently asked questions (FAQs) Getting started 8 Cell handling 8 Medium
More informationWOOD CELL PASSAGING PROTOCOL OVERVIEW
CELL MODEL NAME: Wood Derived from an infiltrating ductal and lobular carcinoma of the breast. Product Category: Cellini Breast Models Catalog #: CB040-000001 Record your lot number here: Please refer
More informationINTERFERin in vitro sirna/mirna transfection reagent PROTOCOL
INTERFERin in vitro sirna/mirna transfection reagent PROTOCOL DESCRIPTION INTERFERin is a powerful sirna/mirna transfection reagent that ensures efficient gene silencing and reproducible transfection in
More informationicell DopaNeurons Application Protocol
icell DopaNeurons Application Protocol Measuring Synchronous Neuronal Activity on the Maestro Multielectrode Array Introduction icell DopaNeurons, human induced pluripotent stem cell (ipsc)-derived neurons,
More informationTransIT-TKO Transfection Reagent
INTRODUCTION TransIT-TKO is a broad spectrum sirna transfection reagent that enables high efficiency sirna delivery and knockdown of target gene expression in many cell types including primary cells. TransIT-TKO
More informationPlastic Storage Bottles Product Selection Guide
Plastic Product Selection Guide A Wide Selection of Bottle Shapes, Sizes, and Materials for All Your Laboratory Needs Corning s disposable bottles are designed for safe, secure storage of tissue culture
More informationµ Slide Membrane ibipore Flow
The ibidi product family is comprised of a variety of µ Slides and µ Dishes, which have all been designed for high end microscopic analysis of fixed or living cells. The high optical quality of the material
More informationProtocols for Neural Progenitor Cell Expansion and Dopaminergic Neuron Differentiation
Protocols for Neural Progenitor Cell Expansion and Dopaminergic Neuron Differentiation In vitro neurological research presents many challenges due to the difficulty in establishing high-yield neuronal
More informationUsing the xcelligence RTCA SP Instrument to Perform GPCR Assays
xcelligence Real-Time Cell Analysis GPCR Assay Protocol Using the xcelligence RTCA SP Instrument to Perform GPCR Assays Overview The xcelligence RTCA SP Instrument The xcelligence Real-Time Cell Analyzer
More informationTransfection of neural stem cells with Lipofectamine Stem Transfection Reagent in StemPro medium
TRANSFECTION PROTOCOL Lipofectamine Stem Transfection Reagent Transfection of neural stem cells with Lipofectamine Stem Transfection Reagent in StemPro medium NSC media, passaging reagents, and complexation
More informationApplication Note. Developed for: Aerius, Odyssey Classic, Odyssey CLx, and Odyssey Sa Infrared Imaging Systems
Application Note On-Cell Western Plate-Based Assay for Targeted Near-Infrared-Labeled Optical Imaging Agent Development: Receptor-Based Binding and Competition Assays Developed for: Aerius, Odyssey Classic,
More informationCulture of Human ipsc-derived Neural Stem Cells in a 96-Well Plate Format
Protocol supplement version 1.0 Culture of Human ipsc-derived Neural Stem Cells in a 96-Well Plate Format Protocol Supplement This protocol supplement is to be used in addition to the Neural Stem Cell
More informationTransIT -Jurkat Transfection Reagent
Quick Reference Protocol, MSDS and Certificate of Analysis available at mirusbio.com/2120 INTRODUCTION TransIT -Jurkat Transfection Reagent is specifically optimized to provide exceptional transfection
More informationTranswell Permeable Supports Including Snapwell and Netwell Inserts Instructions for Use
Transwell Permeable Supports Including Snapwell and Netwell Inserts Instructions for Use Life Sciences TABLE OF CONTENTS Introduction..................................... 1 Transwell Insert Product Descriptions...............
More informationCortical Neural Induction Kit. Protocol version 1.0
Cortical Neural Induction Kit Protocol version 1.0 Protocol version 1.0 Table of Contents Product Information 2 Preparation of Reagents 3 Protocol Overview 4 Seeding ipscs 4 Cortical Neural Induction
More informationTransfection of pluripotent stem cells with Lipofectamine Stem reagent in mtesr1 Medium on Geltrex matrix
TRANSFECTION PROTOCOL Lipofectamine Stem Transfection Reagent Transfection of pluripotent stem cells with Lipofectamine Stem reagent in mtesr1 Medium on Geltrex matrix PSC growth medium, passaging reagents,
More informationClonaCell -CHO. Semi-Solid Cloning Testing Guidelines
ClonaCell -CHO Semi-Solid Cloning Testing Guidelines Table of Contents 1.0 Introduction... 3 2.0 Before Planning a Semi-Solid Cloning Protocol... 4 3.0 Equipment and Materials... 5 4.0 Storage of Semi-Solid
More informationTransIT -Keratinocyte Transfection Reagent
TransIT -Keratinocyte Transfection Reagent INTRODUCTION TransIT -Keratinocyte Transfection Reagent is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in keratinocyte
More informationAccelerating Scientific Discovery
Elite TM Fluorescent Membrane Potential Dye Kit CATALOG NUMBER: CA-M165 Description camp is a key second messenger involved extensively in cellular signal transduction pathways associated with the majority
More informationHuman Pluripotent Stem Cell Growth Medium DXF
Human Pluripotent Stem Cell Growth Medium DXF Instruction Manual Product Size Catalog Number hpsc Growth Medium DXF 500 ml C-28060 Recommended for Human Pluripotent Stem Cells (hpsc) Product Description
More informationTransIT -mrna Transfection Kit
INTRODUCTION TransIT -mrna Transfection Kit is designed to transfect RNA into a broad range of cell types with minimal cellular toxicity. RNA delivery avoids transcriptional regulation effects by directly
More informationTranswell Permeable Supports
Transwell Permeable Supports Including Snapwell and Netwell Inserts Instructions for Use Life Sciences TABLE OF CONTENTS Introduction.................................... 1 Transwell Insert Product Descriptions.............
More informationCellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics User Manual
Takara Bio Europe AB Cellartis DEF-CS 500 Xeno-Free 3D Spheroid Culture Medium w/o Antibiotics Cat. Nos. Y30046, Y30047, Y30048 (061616) Takara Bio Europe AB A Takara Bio Company Arvid Wallgrens backe
More informationTransfection Kit Protocol for MIR 2900, 2904, 2905, 2906
TransIT-HeLaMONSTER Transfection Kit INTRODUCTION TransIT-HeLaMONSTER Transfection Kit is specifically optimized to provide exceptional transfection efficiency of plasmid DNA in HeLa cells and cell types
More informationCardiomyocyte Differentiation Methods
Version 1.1 2018 Allen Institute for Cell Science Cardiomyocyte Differentiation Methods The methods described below were adapted from the small molecule protocol described in Lian et al. 1, and have been
More informationNutriStem V9 XF Medium
Stem Cells NutriStem V9 XF Medium A defined, xeno-free (XF), serum-free (SF) culture medium for hpsc using vitronectin Instructions for Use Product Description NutriStem V9 XF medium is a defined, xeno-free,
More informationAmaxa 4D-Nucleofector Protocol for Undifferentiated Human Mesenchymal Stem Cells [MSC] For 4D-Nucleofector X Unit Transfection in suspension
Amaxa 4D-Nucleofector Protocol For 4D-Nucleofector X Unit Transfection in suspension Self-isolated or Poietics Human Mesenchymal Stem Cells from bone marrow [Lonza, Cat. No. PT- 2501] Example for Nucleofection
More informationCAROUSEL CELL PASSAGING PROTOCOL OVERVIEW
CELL MODEL NAME: Carousel Derived from an endometrioid adenocarcinoma of the ovary. Record your lot number here: Product Category: Raphael Ovarian Models Catalog #: AMS.CB010-000001 Please refer to Certificate
More informationBeginning-to-end Solutions for Drug Discovery
At Corning, we continuously strive towards improving efficiencies and developing new products and technologies for life science researchers. From assay preparation to storage, our technical experts understand
More informationBD MOSAIC h MSC CELL CULTURE ENVIRONMENT DEFINED SERUM FREE
BD MOSAIC h MSC CELL CULTURE ENVIRONMENT DEFINED SERUM FREE As the promise of stem cell therapy grows, so does the need for reliable tools for cell expansion in both research and clinical applications.
More informationAccumax Cell Dissociation Solution
http://www.accutase.com/accumax.html 2015/01/30 Accumax Cell Dissociation Solution Accumax is a ready to use non-mammalian, non-bacterial replacement for all applications of trypsin and collagenase in
More informationIncuCyte Phagocytosis Assay
IncuCyte Phagocytosis Assay For quantification of phagocytosis of apoptotic and non-apoptotic cell This protocol is intended for the measurement of both apoptotic (efferocytosis) and non-apoptotc phagocytosis
More informationIncuCyte phrodo Red Phagocytosis Assay
IncuCyte phrodo Red Phagocytosis Assay For quantification of phagocytosis of apoptotic and non-apoptotic cell This protocol is intended for the measurement of both apoptotic (efferocytosis) and non-apoptotic
More informationTissue Culture Products
Tissue Culture Products PRODUCT CATALOGUE We are a fast-growing, dynamic centre of excellence in the field of innovative, high-quality laboratory products. We specialise in Tissue Culture, distinguishing
More informationIncuCyte Phagocytosis Assay
IncuCyte Phagocytosis Assay For quantification of phagocytosis of apoptotic and non-apoptotic cell This protocol is intended for the measurement of both apoptotic (efferocytosis) and non-apoptotc phagocytosis
More informationab CytoPainter MitoBlue Indicator Reagent
Version 1 Last updated 27 April 2017 ab219940 CytoPainter MitoBlue Indicator Reagent For staining mitochondria in live cells using our proprietary MitoBlue Indicator fluorescence probe This product is
More informationImportant: Please read these instructions carefully
Important: Please read these instructions carefully The viability of these cells is warranted for 30 days from date of shipment when specified reagents and growth conditions are followed as described in
More information