Begin with the supplemental experiment handout and get all experiments set up first before beginning slide and model observations in Exercise 4.
|
|
- Erick Bradley
- 6 years ago
- Views:
Transcription
1 The Cell: Division (Mitosis & Cytokinesis) and Cellular Respiration Exercise 4 (begins page 30 in 8 th edition, page 39 in 9 th 10 th 11 th and 12 th editions) and Supplemental Experiment Handout Anaerobic Metabolism: Fermentation Lab 3 Objectives Read lab Exercise 4 Supplement: do cellular respiration experiment For Exercise 4: do Activity 6 Begin with the supplemental experiment handout and get all experiments set up first before beginning slide and model observations in Exercise 4. Supplemental Experiment Handout: Anaerobic Metabolism: Fermentation Work in groups of 4 people and divide up the work: Follow the directions to set up the experiment. Exercise 4: The Cell: Anatomy and Division (Activity 6 in all editions) Each student will need to use: whitefish blastula slide Identify states and features of mitosis on diagrams and slides (Activity 6, Fig. 4.4): Interphase nuclear envelope mitotic spindle Prophase chromatin metaphase plate Metaphase centrosomes/centrioles Anaphase chromosomes Telophase chromatid Cytokinesis centromere For study: Review Sheet Exercise 4 pages in 8 th edition pages in 9 th 10 th 11 th and 12 th editions Answers in the Instructors Manual at the Eastern Campus Library on reserve Histology photos and unlabeled art to create study sheets is available at www2.sunysuffolk.edu/czuraa Amy Warenda Czura, Ph.D. 1 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
2 ATP Production in Cells Anaerobic Metabolism: Fermentation Cells require energy to drive cellular functions, maintenance, repair, and division. Cells acquire this energy by performing oxidation and reduction reactions on food molecules such as glucose. This energy is then stored in the form of ATP to be used as needed by the cell. The reactions necessary to produce ATP involve enzymes, biological catalysts, that facilitate the reactions to occur in a step-wise fashion to insure that the energy production and release is controlled and does not damage the cell. Additionally, enzymes speed up chemical reactions insuring efficient energy production at temperatures and pressures compatible with life. Enzymes however require optimal conditions to catalyze reactions. The cell, and even the physiology of a whole person, must maintain homeostasis at the optimal conditions for the catalytic power of the cellular enzymes or the chemical reactions necessary for life will cease. In this experiment, we will investigate the cellular process of anaerobic metabolism called Glycolysis, a nearly universal pathways utilized by almost all cells from humans to bacteria. Glycolysis (literally translated as sugar-breaking ) is the ten-step process by which a six-carbon glucose molecule is oxidized into two three-carbon pyruvate molecules resulting in the production of two ATP molecules for use by the cell (see Figure 1). During this process, two cofactor molecules of NAD + are reduced into NADH. The resulting NADH will need to be oxidized back into NAD + to complete the process. When oxygen is available, this is usually done in the Electron Transport Chain resulting in the production of additional ATP by oxidative phosphorylation in a process called aerobic cellular respiration. When oxygen is not available, such as in skeletal muscles cells during rigorous activity, the oxidation of NADH will be done in conjunction with reduction of pyruvate in a fermentation process resulting in the formation of waste products such as lactic acid (see Figure 2). Since it is difficult to study the activity of human cells in the laboratory, we will investigate a similar process in yeast. Yeasts are eukaryotes like humans and perform many of the same chemical reactions as human cells. During anaerobic metabolism, yeasts will perform the same Glycolysis reactions as human cells, however a different set of enzymes will convert the NADH and pyruvate into ethanol and carbon dioxide instead of lactic acid at the end (see Figure 3). As with human cells, the enzymes catalyzing a reaction at each step in this pathway function best at the optimal temperature of 37 C, at a nearly neutral ph, and in the presence of the cofactor Mg 2+. Using glucose as the substrate, the efficiency of enzyme function can be quantified by measuring the accumulation of the waste product carbon dioxide as the glycolysis reactions progress. Amy Warenda Czura, Ph.D. 2 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
3 Figure 1: Glycolysis Pathway Amy Warenda Czura, Ph.D. 3 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
4 Figure 2: Cellular Respiration and Fermentation for ATP Production Figure 3: Fermentation Reactions Amy Warenda Czura, Ph.D. 4 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
5 Experimental Procedure: Set up and incubate respirometer tubes as follows 1. Organize in numerical order in the test tube rack the 6 plastic conical 15 ml tubes labeled 1-6 on the bases. Label the six large (25 x 150 mm) glass tubes #1-#6 with a wax pencil and include group initials to identify the tube later. 2. Using squeezable transfer pipettes for dispensing and the milliliter lines on the plastic conical tubes for volumetric measuring, add reagents to the 15 ml conical tubes according to the table below. Be sure to use different transfer pipettes for each different reagent and do not contaminate the stock containers when acquiring and dispensing fluids. a. Shake to mix up the yeast suspension before removing a sample to insure high and equal cell concentration gets delivered to each tube. Remove the appropriate quantity of yeast cells immediately after resuspension of the stock container. b. Add yeast first filling to the 5 ml line on the conical tube. Tubes 1-5 all contain the same live yeast, however do note that tube 6 contains a different yeast sample that was boiled for 10 minutes prior to the start of the experiment. c. Add glucose second, 5 ml, to tubes 2-6, filling to the 10 ml line. d. Add Magnesium third, 1 ml, to tubes 3-6, filling to the 11 ml line. e. Add acid fourth, 2 ml, to tube 5, filling to the 13 ml line. Anaerobic Metabolism Respirometer Tubes SOLUTION TUBE 1 TUBE 2 TUBE 3 TUBE 4 TUBE 5 TUBE 6 4% Live Yeast 5 ml 5 ml 5 ml 5 ml 5 ml 0 4% Boiled Yeast ml 2.5% Glucose 0 5 ml 5 ml 5 ml 5 ml 5 ml 0.1M MgSO ml 1 ml 1 ml 1 ml 1M HCl ml 0 Incubation Temperature 37 C 37 C 37 C 0 C 37 C 37 C 3. Once all the tubes contain the appropriate solutions, fill each conical tube to the clear, unmarked 15 ml line with distilled water using the squeeze bottle. (The clear 15 ml line is above the blue 14 ml line but below the threads for the screw cap.) 4. Cover each conical tube with the screw cap and mix by inverting several times to insure the ingredients are uniformly distributed. Remove the cap. 5. To assemble the respirometer, hold the filled conical tube upright and slip the corresponding large glass tube over it. Keep one finger at the tip of the filled conical tube and press it firmly against the bottom of the larger outer tube. (See the figure 4 on the next page) 6. Quickly invert the respirometer and measure the height of the air bubble at the top of the small conical tube in milliliters using the blue labeled lines. Record this number in the data chart. (If you were careful and have no spillage, the bubble should be about even with the 2 ml line.) Amy Warenda Czura, Ph.D. 5 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
6 Figure 4: Respirometer Set-up 7. Repeat steps 5 & 6 for all six tubes. 8. Place each respirometer in the appropriate water bath for the incubation temperature indicated in the chart. (Tubes 1, 2, 3, 5, 37 C, Tube 0 C). Be certain you have the initial bubble height recorded in the data chart for each tube. 9. Check the progress of the respirometers every 30 minutes. After about 2 hours (or when large bubbles have formed in the most active tubes), remove the respirometers and record the final height of the bubble. It is normal for fluid to have been forced out of the small tubes during incubation and for this fluid to pool in the bottom of the large tubes. 10. Place all materials and equipment in the appropriate discard area and analyze your results. Data Table Start time: End Time: Total incubation time (hr): TUBE Initial height of bubble (ml) Final height of bubble (ml) Total height of CO 2 produced (ml)* Rate of CO 2 production (ml/hr)** *Total height of CO 2 produced is calculated by subtracting the initial height from the final height. **Rate of CO 2 production (an indicator of reaction rate) is calculated by dividing the total height of CO 2 produced in milliliters by the total time of incubation. Amy Warenda Czura, Ph.D. 6 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
7 Understanding the Experiment Thought Questions: 1. Which tube would you expect to have a greater reaction rate, tube 3 or tube 4? Why? 2. Do you expect tube 6 to produce as much CO 2 as tube 3? Why or why not? 3. Why would you predict tube 1 to show little to no reaction occurring? Amy Warenda Czura, Ph.D. 7 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
8 4. Would you expect the reaction rate to be faster in tube 2 or tube 3? Why? 5. Which tube has an acidic environment? How do you expect this to impact enzyme activity? 6. Based on the results you observed for your experiment, what are the necessary conditions for optimal glycolysis enzyme activity? Amy Warenda Czura, Ph.D. 8 SCCC BIO130 Lab 3 Mitosis & Cellular Respiration
AP Biology: Fermentation, Respiration, & Enzyme Specificity
Name AP Biology: Fermentation, Respiration, & Enzyme Specificity Introduction: All living cells, including the cells in your body and the cells in yeast, need energy for cellular processes such as pumping
More information1 Exchange with the Environment
CHAPTER 2 1 Exchange with the Environment SECTION The Cell in Action BEFORE YOU READ After you read this section, you should be able to answer these questions: How do cells take in food and get rid of
More informationRespiration. The process of cellular respiration can be summarized by the following equation:
Respiration Objectives 1. To describe oxidation and reduction in terms of electron and H + transfer. 2. To distinguish anaerobic from aerobic cellular respiration in terms of ATP, oxygen, and chemiosmosis.
More informationCell Division. Use Target Reading Skills. This section explains how cells grow and divide.
Name Date Class Cell Processes Guided Reading and Study Cell Division This section explains how cells grow and divide. Use Target Reading Skills As you read, make a cycle diagram that shows the events
More informationKansas Corn: Fermenting Fuel- Designing a Procedure for Fast Fermentation
Kansas Corn: Fermenting Fuel- Designing a Procedure for Fast Fermentation This lab is made possible with the support and content contributions of the Kansas Corn Commission. Kansas Corn: Fermenting Fuel
More informationRespiration Worksheet. Respiration is the controlled release of energy from food. Types of Respiration. Aerobic Respiration
Respiration Worksheet Respiration is the controlled release of energy from food! The food involved in respiration is usually! Internal respiration is controlled by which allow energy to be released in!
More informationFermentation. OpenStax College. 1 Lactic Acid Fermentation
OpenStax-CNX module: m45440 1 Fermentation OpenStax College This work is produced by OpenStax-CNX and licensed under the Creative Commons Attribution License 3.0 By the end of this section, you will be
More informationAlcoholic Fermentation in Yeast
Lab 5. Alcoholic Fermentation in Yeast Prelab Assignment Before coming to lab, read carefully the introduction and the procedures of this experiment, and then answer the prelab questions at the end of
More informationPage Monday December 19, 2016
replication and Page 5-55 Monday December 9, 06 Notebook check 0: exam review notebook pages 5-55 Proteins Enzymes Activation energy Photo Cellular respiration, replication and Learning goal: Describe
More informationChapter 12 Respiration
2.2 Cell Metabolism Learning Objectives Chapter 12 Respiration 2.2.5 Respiration 1. Define, give the role and balanced equation for "aerobic respiration". 2. Explain the stages and molecules involved in
More information2. Know the parts of a light microscope and general rules for using and focusing a microscope, such as:
SNC 2DI Exam Review: Biology Unit 1. Understand the meaning of the following terms. Be able to recognize their definitions: Biology Mounting medium Telophase Organelle Cell Theory Cell cycle Cytokinesis
More informationBIOLOGY 311C - Brand Spring 2008
BIOLOGY 311C - Brand Spring 2008 NAME (printed very legibly) Key UT-EID EXAMINATION 3 Before beginning, check to be sure that this exam contains 7 pages (including front and back) numbered consecutively,
More informationPhotosynthesis (in chloroplasts) Solar energy 6CO2 + 6H2O
B-3.1 Summarize the overall process by which photosynthesis converts solar energy into chemical energy and interpret the chemical equation for the process. Photosynthesis (in chloroplasts) Solar energy
More informationQuarter 1 Review Biology
Quarter 1 Review Biology TEKS: 2ABCD, 3A, 4AB, 6 ABCDEF, 9ABC Topics: Safety & Scientific Processes, Biochemistry (Inorganic & Organic), DNA (Replication), Cell Division Use the picture to the left to
More informationSunlight (solar energy) CO2 + H2O. Cellular Respiration (mitochondria) 36 ATP
Aerobic & Anaerobic Conditions HASPI Medical Biology Lab 11 Background/Introduction The Flow of Energy & Cycling of Matter Photosynthesis and cellular respiration (including aerobic and anaerobic respiration)
More information2. Know the parts of a light microscope and general rules for using and focusing a microscope, such as:
SNC 2DI Exam Review: Biology Unit 1. Understand the meaning of the following terms. Be able to recognize their definitions: Biology Mounting medium Telophase Organelle Cell Theory Cell cycle Cytokinesis
More informationRespiration. 2.2 Cell Metabolism Objectives At the end of this sub section students should be able. to:
Name: 2.2 Cell Metabolism Objectives At the end of this sub section students should be able 2.2.5 1. Definition of the term: aerobic respiration. 2. Explain the role of aerobic respiration what does it
More informationHow Is a Marathoner Different from a Sprinter?
How Is a Marathoner Different from a Sprinter? Muscles in human legs contain two different types of muscle fibers Marathoners have more slow-twitch fibers, which perform better in endurance exercises Sprinters
More informationCell Biology Homework
Cell Biology Homework NAME: CLASS: 1 1. The diagram below shows two cells. Cell Structure a) Complete the table below to give the name and functions of the parts labelled A, B and C. (2) Letter Part Function
More informationBELLRINGER. Name three enzymes in the human body.
BELLRINGER Some reactions have enzymes. What is an advantage to having an enzyme for a particular reaction? What is a disadvantage to having an enzyme for a particular reaction? Name three enzymes in the
More informationWHAT IS AN ENZYME? Most enzymes are proteins Act as a catalyst to speed up a chemical reaction by helping molecules react with each other faster
BELLRINGER-3/9/15 1. Based on your homework reading, what is an enzyme? 2. Some reactions have enzymes. What might be an advantage to having enzymes in chemical reactions? Any disadvantages? 3. Can you
More informationHole s Essentials of Human Anatomy & Physiology
Hole s Essentials of Human Anatomy & Physiology David Shier Jackie Butler Ricki Lewis Created by Dr. Melissa Eisenhauer Head Athletic Trainer/Assistant Professor Trevecca Nazarene University Amended by
More informationMost Probable Number (MPN) & Biological Oxygen Demand (BOD)
Most Probable Number (MPN) & Biological Oxygen Demand (BOD) Part : Presumptive Coliform Test (MPN) Introduction This lab exercise will employ a commonly used multi-tube fermentation technique. The results
More informationEOQ 1 Review Biology
EOQ 1 Review Biology TEKS: 2ABCD, 3A, 4AB, 6 ABCDEF, 9ABC Topics: Safety & Scientific Processes, Biochemistry (Inorganic & Organic), DNA (Replication), Cell Division Use the picture to the left to answer
More informationKansas Corn: Ethanol - Corn Mash and Distillation High School Student Lab Packet
Kansas Corn: Ethanol - Corn Mash and Distillation High School Student Lab Packet Overview In this lab, students will learn about ethanol and its important role in our world s ever-increasing demand for
More informationKansas Corn: Ethanol - Corn Mash and Distillation High School Student Lab Packet
Kansas Corn: Ethanol - Corn Mash and Distillation High School Student Lab Packet Overview In this lab, students will learn about ethanol and its important role in our world s everincreasing demand for
More informationThe Cell Cycle. Study Guide: Cell Division and DNA Structure
A. Review Materials 1. Text: Chapter 2, section 3. 2. EOS questions: cell division and DNA (online) 3. Any videos linked from the website 4. Labs: review conclusions and notes (DNA Candy, Flipbooks, Scum)
More informationHuman Anatomy & Physiology I Dr. Sullivan Unit IV Cellular Function Chapter 4, Chapter 27 (meiosis only)
Human Anatomy & Physiology I Dr. Sullivan Unit IV Cellular Function Chapter 4, Chapter 27 (meiosis only) I. Protein Synthesis: creation of new proteins a. Much of the cellular machinery is devoted to synthesizing
More informationREVIEW SHEET: Units 9 & 10 Cell Cycle, DNA, & Gene Expression
REVIEW SHEET: Units 9 & 10 Cell Cycle, DNA, & Gene Expression HONORS BIOLOGY Textbook Reading: Cell Cycle (Ch. 10.1 and 10.2), DNA (Ch. 12), and Gene Expression (Ch. 13) Handouts:! Online Tutorial: Cell
More informationseven mm screw top bottles Vernier computer interface shallow pan Vernier Dissolved Oxygen Probe scissors Logger Pro
Primary Productivity AP Bio Lab 12B Name: Date: Oxygen is vital to life. In the atmosphere, oxygen comprises over 20% of the available gases. In aquatic ecosystems, however, oxygen is scarce. To be useful
More informationNational 5 Biology - Unit 1 Cell Biology
1. Cell Structure National 5 Biology - Unit 1 Cell Biology Cells are the basic unit of life Stains can be used to make cell structures visible Organelles are present in cell cytoplasm and carry out a specialised
More informationMicrobial Growth and The Control of Microbial Growth (Chapter 6 & 7)
Microbial Growth and The Control of Microbial Growth (Chapter 6 & 7) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Eastern Campus Primary Source for figures and content:
More informationLab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab
Lab Exercise: Examining Water Quality: Most Probable Number & Colilert Test Kit Lab OBJECTIVES 1. Understand the use of MPN to determine likely fecal water contamination. 2. Understand the use of MUG,
More informationCells and Tissues. Overview CELLS
Cells and Tissues WIll The basic unit of structure and function in the human body is the cell. Each of a cell's parts, or organelles, as well as the entire cell, is organized to perform a specific function.
More informationDNA and Chromosomes. 2. What molecules make up the rungs of the ladder? 3. What keeps the two sides of the ladder paired?
DNA and Chromosomes Objectives: At the end of this lab you should be able to: 1. Describe the structure and function of DNA. 2. Know which bases in DNA pair with each other. 3. Define the terms chromatid,
More informationUnit 4: Cell Development and Replication, Part I: The Cell Cycle
Name: Block: PACKET #9 Reading: Unit 4: Cell Development and Replication, Part I: The Cell Cycle Objectives: By the conclusion of this unit you will be able to: Date: Introduction to the cell cycle 1.
More informationName period date AP BIO- 2 nd QTR 6 Week Test Review
Name period date AP BIO- 2 nd QTR 6 Week Test Review Cellular Respiration 1. In the following reaction, C6H12O6 + 6 O2 6 CO2 + 6 H2O + Energy, identify which is reduced and which is oxidized: a. Glucose
More information1. I can describe the stages of the cell cycle.
Unit 5 Study Guide Cell Cycle pg. 1 1. I can describe the stages of the cell cycle. Interphase = period in between division G1 = growth phase S = DNA replication G2 = Preparation for division (extra copies
More informationVocab Word 1: Interphase
Vocab Word 1: Interphase Interphase is the phase of the cell cycle in which a typical cell spends most of its life. During this phase, the cell copies its DNA in preparation for mitosis. Interphase is
More informationSCHEDULE. Friday: Pet Investigations: Plate counts - how to know how many clones of your pet you have (pg. 9-10)
SCHEDULE Wednesday: Pet Investigations: Phenol Red Broth with Durham tubes (pg. 3-4) Oxidation/Fermentation Agar (pg. 5-6) Anaerobic Growth (pg. 7) Growth in Liquid Culture (pg. 8-9) Friday: Pet Investigations:
More informationEXPERIMENT. Biochemical Testing for Microbial Identification Methyl Red, Voges- Proskauer, and Catalase Testing
EXPERIMENT Biochemical Testing for Microbial Identification Methyl Red, Voges- Proskauer, and Catalase Testing Hands-On Labs, Inc. Version 42-0246-00-02 Review the safety materials and wear goggles when
More informationTopic 7. Respiration. . The electron transport chain occurs in the cristae of the mitochondria. High
Topic 7. Respiration Respiration is the cellular process by which some of the chemical energy of a reduced carbon substrate is converted into the chemical energy of ATP. While it seems counter intuitive,
More informationCell Division. embryo: an early stage of development in organisms
Over the past several years, a debate has been brewing over the use of stem cells. Stem cells can be used to treat certain diseases and conditions such as spinal cord injuries, diabetes, arthritis, and
More informationChapter 9 Cellular Respiration Worksheet Answer Key
We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with chapter 9 cellular respiration
More informationA Discovery Laboratory Investigating Bacterial Gene Regulation
Chapter 8 A Discovery Laboratory Investigating Bacterial Gene Regulation Robert Moss Wofford College 429 N. Church Street Spartanburg, SC 29307 mosssre@wofford.edu Bob Moss is an Associate Professor of
More informationUnit 4 Information NUCLEIC ACIDS DNA GENES DOUBLE HELIX REPLICATION MITOSIS
Unit 4 Information NUCLEIC ACIDS DNA GENES DOUBLE HELIX REPLICATION MITOSIS 1/2/2018 Bell Work What is a Gene? Genes as Medicine As you watch the film, complete the questions on the film guide. When you
More informationTake-Home Quiz II. Summer 2005 Semester
General Instructions and Information: Obtain an answer sheet from the instructor and legibly write your name in the appropriate space. After placing your name, you must enter your Patron ID Number (NOT
More informationA. Incorrect! Enzymes are not altered or consumed by the reactions they catalyze.
CLEP Biology - Problem Drill 04: Enzymes and Cellular Metabolism No. 1 of 10 1. Which of the following statements about enzymes is correct? (A) Enzymes are consumed in a reaction. (B) Enzymes act by lowering
More informationSection 10. Junaid Malek, M.D.
Section 10 Junaid Malek, M.D. Cell Division Make sure you understand: How do cells know when to divide? (What drives the cell cycle? Why is it important to regulate this?) How is DNA replication regulated?
More informationWhat Are the Yeast Cells Doing?
Cell Division Reading Preview Key Concepts What events take place during the three stages of the cell cycle? How does the structure of DNA help account for the way in which DNA copies itself? Key Terms
More informationLab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl)
Lab Activity #14 - Bacteriological Examination Of Water and Milk (Adapted from Lab manual by Dr. Diehl) Some of the diseases that humans can contract from drinking polluted water include typhoid, dysentery,
More informationB4 Life processes Q1 Question Label the animal cell. B4 Life processes. Question: Label the plant cell.
Q1 Label the animal cell. Q2 : Label the plant cell. A cell membrane, B cytoplasm, C nucleus, D- mitochondria A cell wall, B vacuole, C chloroplast, D nucleus, E cell wall, F cytoplasm, G - mitochondria
More informationBiology Spring Final Study Guide
Biology Spring Final Study Guide The final will cover all information since the beginning of the school year, but the emphasis will be second semester (approx 20% 1 st semester, 80% 2 nd semester information,
More informationBiotechnology In Your Mouth
PR005 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Biotechnology In Your Mouth Teacher s Guidebook (Cat. # BE 102) think proteins! think
More informationEnzymes and Enzymatic Reactions Adapted with permission from the original author, Charles Hoyt
Biol. 261 Enzymes and Enzymatic Reactions Adapted with permission from the original author, Charles Hoyt Introduction All living things use energy, give off waste, reproduce and interact with the environment.
More informationKansas Corn: Ethanol - Corn Mash and Distillation
Kansas Corn: Ethanol - Corn Mash and Distillation This lab is made possible with the support and content contributions of the Kansas Corn Commission. Grade Level: High School Overview In this lab, students
More information1. Carry the microscope in an upright position with both hands and place the base of the microscope 5cm from the edge of the bench
The Microscope Operating the compound light microscope 1. Carry the microscope in an upright position with both hands and place the base of the microscope 5cm from the edge of the bench 2. Check that lenses
More informationReinforcement. Cells and Life CHAPTER 1 LESSON 1
Reinforcement Cells and Life LESSON 1 Directions: In numbers 1 through 4 below, a code letter has been substituted for each letter of the alphabet. To find out what the sentence says, use the following
More informationNAD/NADH Cell-Based Assay Kit
NAD/NADH Cell-Based Assay Kit Item No. 600480 Customer Service 800.364.9897 * Technical Support 888.526.5351 www.caymanchem.com TABLE OF CONTENTS GENERAL INFORMATION 3 Materials Supplied 3 Safety Data
More information4/6/2015. Bacterial Growth and Nutrition. Nutrients + Oxygen. Temperature. Temperature
Bacterial Growth and Nutrition ph Nutrients + Oxygen Temperature Temperature 1 Environmental Oxygen Requirements -- can support or hinder growth 1. Aerobic need high oxygen concentration to grow 2. Anaerobic
More informationAlgae Balls. Investigating Photosynthesis. Student Materials. Introduction Lab Protocol Data Collection Worksheet... 5
Algae Balls Investigating Photosynthesis Student Materials Introduction... 2 Lab Protocol... 4 Data Collection Worksheet... 5 Pre-Lab Questions... 8 Post-Lab Questions and Analysis... 9 Last updated: October
More informationGB Translated English of Chinese Standard: GB
Translated English of Chinese Standard: GB4789.35-2016 www.chinesestandard.net Sales@ChineseStandard.net GB NATIONAL STANDARD OF THE PEOPLE S REPUBLIC OF CHINA GB 4789.35-2016 National food safety standard
More informationInvestigating Bean Plant Respiration
Investigating Bean Plant Respiration SC Academic Standards: 5.L.4B; 6.L.4A; 6.L.4B; 6.L.5; 7.L.3A; 7.L.3B; H.B.3; NGSS DCI: 5- LS1.C; 5- LS2.B; MS- LS1.C; MS- PS3.D; HS- LS1.C Science and Engineering Practices:
More informationCellular Energetics. Photosynthesis. Photosynthesis. Photosynthesis. Photosynthesis. Photosynthesis. Photosynthesis, Respiration, Fermentation
Cellular Energetics,, Defition = process by which plants make their own food Requires light ultraviolet Where does it take place? chloroplast What pigment makes it possible? chlorophyll What types of organisms
More informationCELLULAR PROCESSES; REPRODUCTION. Unit 5
CELLULAR PROCESSES; REPRODUCTION Unit 5 Cell Cycle Chromosomes and their make up Crossover Cytokines Diploid (haploid diploid and karyotypes) Mitosis Meiosis What is Cancer? Somatic Cells THE CELL CYCLE
More informationLab 5 Cell Biology II
Lab 5 Cell Biology II Learning Objectives Describe the structures and processes required for protein synthesis. Define a gene. Compare chromosomes and chromatin. Describe the process of DNA replication.
More informationTeacher Preparation Notes for Alcoholic Fermentation in Yeast A Bioengineering Design Challenge 1
Teacher Preparation Notes for Alcoholic Fermentation in Yeast A Bioengineering Design Challenge 1 This multi-part minds-on, hands-on activity helps students to understand both alcoholic fermentation and
More informationSt Andrew s High School, Coatbridge Biology Department. National 5 Unit 1 Cell Biology Summary Notes
St Andrew s High School, Coatbridge Biology Department National 5 Unit 1 Cell Biology Summary Notes Key area 1.1 : Cell Structure. Key area 1.2: Transport across Cell Membrane. Key area 1.3: DNA and the
More informationHow Can Chemical Reactions Be Used to Improve Air Quality?
5.2 Explore How Can Chemical Reactions Be Used to Improve Air Quality? One way to reduce the pollutants in the air is by keeping them from getting into the air in the first place. Some pollutants are solid
More informationDNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003
DNA TRANSFORMATION OF BACTERIA RED COLONY REVISED 3/2003 Prepared by the Office of Biotechnology, Iowa State University TEACHER PREPARATION AND INSTRUCTION GUIDE Preparation for the DNA transformation
More informationChapter 5: Microbial Metabolism (Part I)
Chapter 5: Microbial Metabolism (Part I) Microbial Metabolism Metabolism refers to all chemical reactions that occur within a living organism. These chemical reactions are generally of two types: Catabolic:
More informationth is is re vi sio n. com
Section (a) Cells and organelles th is is re vi sio n. com 1. Make a diagram to show the structure of a liver cell as seen using an electron microscope. On your diagram, label the following: nucleus nuclear
More informationQ1. Figure 1 shows a pair of chromosomes at the start of meiosis. The letters represent alleles. Figure (1)
Q1. Figure 1 shows a pair of chromosomes at the start of meiosis. The letters represent alleles. Figure 1 (a) What is an allele? (b) Explain the appearance of one of the chromosomes in Figure 1. (c) The
More informationMetabolism BIOL 3702: Chapter 10
Metabolism BIOL 3702: Chapter 10 Introduction to Metabolism u Metabolism is the sum total of all the chemical reactions occurring in a cell u Two major parts of metabolism: v Catabolism Ø Large, more complex
More informationThe Effect of Alpha Lipoic Acid (ALA) on S. cerevisiae Cell Proliferation
American Journal of Medical and Biological Research, 2018, Vol. 6, No. 2, 21-26 Available online at http://pubs.sciepub.com/ajmbr/6/2/1 Science and Education Publishing DOI:10.12691/ajmbr-6-2-1 The Effect
More information1 Yeast Lab Introduction
1 Yeast Lab Introduction Population modeling is an important problem. Unfortunately we do not have the resources for you to practice modeling populations like deer, rabbits, or even mosquitoes. In order
More informationNational 5 Unit 1: Cell Biology Topic 1.1 Cell Structure. Which part of the cell is composed of cellulose? 1
National 5 Unit 1: Cell Biology Topic 1.1 Cell Structure 1. The diagram below shows parts of a plant cell. Which part of the cell is composed of cellulose? 1 2. Which structural feature is found in a plant
More informationKansas Corn: Fermentation in a Bag
Kansas Corn: This lab is made possible with the support and content contributions of the Kansas Corn Commission. Kansas Corn: rade Level: Middle School Overview Any educator electing to perform demonstrations
More informationCells and Energy. e. Aerobic. a. Photosynthesis c. Thylakoids
Name: Date: Period: BIO FIRST SEMESTER STUDY GUIDE & 1 st Semester Final Exam Study Packet All the information below can be found in your notes or textbook. Remember this is only a guide. There may be
More informationCricket Respiration. Measuring the Effect of Temperature on Ectotherms
Biology Cricket Respiration Measuring the Effect of Temperature on Ectotherms MATERIALS AND RESOURCES EACH GROUP TEACHER aprons beaker, 1000 ml balance hot plate beaker, 1000 ml beaker, 600 ml calculator,
More informationStandards: Common Core State Standards, Arkansas State Frameworks. Science Compare and contrast prokaryotes and eukaryotes
Title: Author: Course: Methanogenesis in Earth and Body Candace Campbell Brookland High School Brookland Anatomy and Physiology, Biology, Life Science Duration: Two class periods Grade Level: 9-12 Objective:
More information1. I can describe the stages of the cell cycle.
Unit 5 Study Guide Cell Cycle pg. 1 1. I can describe the stages of the cell cycle. Interphase = period in between division G1 = growth phase S = DNA replication G2 = Preparation for division (extra copies
More informationNADP + /NADPH Assay Kit (Colorimetric)
Product Manual NADP + /NADPH Assay Kit (Colorimetric) Catalog Number MET-5018 100 assays FOR RESEARCH USE ONLY Not for use in diagnostic procedures Introduction Nicotinamide adenine dinucleotide phosphate
More informationUnit 1 Human cells. 1. Division and differentiation in human cells
Unit 1 Human cells 1. Division and differentiation in human cells Stem cells Describe the process of differentiation. Explain how differentiation is brought about with reference to genes. Name the two
More informationHow is the Global Increase in Carbon Dioxide Changing the Population Density of Soil. Bacteria? Soil Ecology Project. Madeline Kim. Katherine Harrison
Carbon Dioxide Effect on Soil Bacteria 1 How is the Global Increase in Carbon Dioxide Changing the Population Density of Soil Bacteria? Soil Ecology Project Madeline Kim Katherine Harrison Foula Protopapas
More informationPart 1: What s In A Leaf?
Part 1: What s In A Leaf? Reactants YIELD products Reactants: the substances that are required (go in) Products: the substances that are produced (go out) Part 2: Card Sort Practice Notice that you have
More informationBiochemical Oxygen Demand
Biochemical Oxygen Demand Computer 20 Oxygen available to aquatic organisms is found in the form of dissolved oxygen. Oxygen gas is dissolved in a stream through aeration, diffusion from the atmosphere,
More informationElective Type: Credit Hours: 4 Corequisites: Developmental: (yes/no) Lecture: 3 Clinical: 0 Lab: 3 Studio 0 Other: 0. TOTAL: 6 Other Requirements:
COURSE SYLLABUS Course Title: General Biology I (lecture/lab) Department: Mathematics and Science Curriculum: Biology Date submitted: April 2016 (AAC: 16-20) Course Descriptors: Make certain that the course
More informationCARBOHYDRATE FERMENTATION TEST
Microbiology Laboratory (BIOL 3702L) Page 1 of 6 Principle and Purpose CARBOHYDRATE FERMENTATION TEST Microorganisms need to generate energy in order to grow, divide, and survive. In any given environment,
More information1. ADHERE AND DEFEND: Our bacterium has entered the host. Now it needs to adhere and get past the normal microbiota.
North Seattle College Stage 02 Colonization and Infection This explanatory model will tell the story of how one bacterium adheres to a host and, through binary fission, ends up making two daughter cells.
More informationMetabolism. BIOL 3702: Chapter 10. Introduction to Metabolism. Energy and Work. BIOL 3702: Chapter 10 AY Dr. Cooper 1. Metabolism (cont.
Metabolism BIOL 3702: Chapter 10 Introduction to Metabolism u Metabolism is the sum total of all the chemical reactions occurring in a cell u Two major parts of metabolism: v Catabolism Ø Large, more complex
More information1. What is DNA? 2. List anything you know about DNA. (from readings, class, TV?)
1. What is DNA? 2. List anything you know about DNA (from readings, class, TV?) Before we begin, let s investigate the way DNA molecules are set up! http://learn.genetics.utah.edu/content/molec ules/builddna/
More informationDNA RESTRICTION ANALYSIS
DNA RESTRICTION ANALYSIS In this experiment, DNA from the bacteriophage Lambda (48,502 base pairs in length) is cut with a variety of restriction enzymes and the resulting fragments are separated using
More information1. What is DNA? 2. List anything you know about DNA. (from readings, class, TV?)
1. What is DNA? 2. List anything you know about DNA (from readings, class, TV?) Before we begin, let s investigate the way DNA molecules are set up! http://learn.genetics.utah.edu/content/molec ules/builddna/
More informationDNA is the genetic material found in cells Stands for: Deoxyribonucleic Acid Is made up of repeating nucleic acids It s the Unit of Heredity
What is DNA? DNA is the genetic material found in cells Stands for: Deoxyribonucleic Acid Is made up of repeating nucleic acids It s the Unit of Heredity DNA is found in the cytoplasm of prokaryotes and
More informationReduction of Ferric Compounds by Soil Bacteria
No. 3, Volume 10 of the Jouml of General Microbiology was issued on 10 June 1954. BROMFIELD, S. M. (1954). J. gen. Microbiol. 11, 14. Reduction of Ferric Compounds by Soil Bacteria BY S. M. BROMFIELD Microbiology
More informationHigher Biology. Unit 2: Homework Booklet Metabolism and Survival
Higher Biology Unit 2: Homework Booklet Metabolism and Survival 0 1 Sub Topic 2.1: Regulation of Metabolism 1. Membranes can form small compartments within cells. Small compartments have: A high surface
More informationExam 2 Study Guide. Monday, July 30 th 125 points. 3 short answer questions approximately 7 points each
Exam 2 Study Guide Monday, July 30 th 125 points 3 short answer questions approximately 7 points each No complete sentences required, though you can write them if you want Variable points (denoted next
More informationAlgae Balls. Investigating Photosynthesis. Student Materials. Introduction Lab Protocol Data Collection Worksheet... 5
Investigating Photosynthesis Student Materials Introduction... 2 Lab Protocol... 5 Data Collection Worksheet... 5 Pre-Lab Questions... 8 Post-Lab Questions and Analysis... 9 Last updated: 08/16/2018 Introduction
More informationB1 Quick Revision Questions. B1 for AQA GCSE examination 2018 onwards
B1 Quick Revision Questions Question 1... of 50 Name the 4 components that are found in an Animal Cell Answer 1... of 50 Nucleus, Mitochondria, Cell Membrane & Cytoplasm. Question 2... of 50 Name the other
More information