O1-5 O1-6. Cornelia Haas 1,, Tarek El-Najjar 2, Nikolaus Virgolini 2, Marina Smerilli 2, Markus Neureiter 2
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1 Abstracts / New Biotechnology 33S (2016) S1 S213 S7 because of health protection. The high resistance of these structures to bactericides is associated with, inter alia, the transfer of genes between the bacteria cells. This phenomenon is important for antibiotic resistance proliferation in the environment. Therefore in the presented study the exchange of antibiotic resistance genes between bacteria was observed inside the biofilms formed by strains of Escherichia coli and Pseudomonas putida. Observations of the development of these structures using CLSM were also conducted. The conducted analysis showed that between applied strains genetic exchange takes place, and it runs in two directions. Moreover, the dosage of antibiotics to the culture does not affect the genetic exchange, both the initiating and braking. However the effect of temperature on the frequency of genetic exchange has occurred; in the case of culturing at a temperature lower than the optimum for growth of the bacterial strain genetic transfer occurred much less frequently. In this study, we carried out the analysis of the total protein content in the structures of single-species biofilms and rated their proteolytic activity. The measurement of metabolic activity (XTT) was performed. In addition, microscopic observations in developing structures were carried out, both during life, and stained with DAPI dyes or Live/Dead. As a result, it was possible to observe the distribution of cells in biofilms, especially living and dead. To observe the fluorescence and confocal microscope was used. O1-4 Tropical microalgae isolated on Reunion island (France, Indian Ocean) as sources of antifouling molecules: The BIOPAINTROP project Laurent Dufosse 5,, Alexis Bazire 1, Yves Blache 2, Laurent Bleriot 3, Christine Bressy 2, Jean-François Briand 2, Dalyal Copin 1, Gérald Culioli 2, Maxime Delbury 4, Fabienne Fay 6, Isabelle Grondin 7, Isabelle Linossier 1, Ludovic Mangrie 3, Karine Réhel 8, Pierre Sauleau 8, Thierry Taye 3, Alina Tunin-Ley 9, Jean Turquet 9, Claudia Zea Obando 8, Mayalen Zubia 10 1 Universite de Bretagne Sud, France 2 Universite de Toulon, France 3 Bioalgostral, France 4 NAUTIX, France 5 University of Reunion Island, France 6 Univ UBS, France 7 Universite de La Reunion, France 8 UBS, France 9 Hydrô, France 10 Universite de Polynesie Française, France Biofouling is associated to colonization of artificial submerged structures by aquatic organisms. This process induces adverse effects such as loss of hydrodynamism, corrosion, weight increase of equipments. Numerous toxic compounds (copper, arsenic) have been used during decades to avoid biofouling of ships, until EU has banned them since For this reason, a new strategy, focusing on environmental friendly molecules is requested aiming to provide coatings that progressively release active natural compounds, non-toxic for environment. As a significant component of marine organisms, microalgae are a promising source of active natural substances, with biotechnological potential value. Growing microalgae is a worldwide project for various purposes actually e.g. biofuel. BIOPAINTROP project aims to develop antifouling coatings with active biomolecules originating from tropical marine resources (microalgae) from Reunion Island. Two main objectives have been designated: (i) identification of active molecules from tropical microalgae and (ii) incorporation of these compounds in adequate coatings and confirmation of the efficiency of these products in both temperate and tropical marine environments. To reach the targeted results, a pluridisciplinary group has been set up with 6 French teams with complementary expertises: (i) HYDRÔ based on Reunion island and specialized in tropical marine microalgae, (ii) three University laboratories: LCSNSA (Reunion) specialized in natural products valorisation; LBCM (Bretagne) specialized in marine biotechnologies; MAPIEM (Toulon) specialized in polymer materials engineering and marine biocompounds, (iii) Private partners: NAUTIX producing environmental friendly paints, expert in processing ecological and antifouling coatings; BIOALGOSTRAL a start-up from Reunion specialized in production/valorisation of microalgae biomass. O1-5 Heavy metal recovery through nanoparticle synthesis Matthew Edmundson University of Edinburgh, United Kingdom The presence of heavy metal contaminants in industrial waste and in polluted soils and water is a major problem in Europe and in the wider world. Current methods of decontamination, such as phytoremediation or employing adsorbant materials, can be resource and time intensive. Most methods also have the problem of disposal of the heavy metals recovered from the decontamination process. We are working on solving these issues by using biological methods to convert the recovered metals into industrially useful catalytic nanoparticles, thereby incentivising decontamination by producing high-value materials from the contaminants themselves. To this end we are developing synthetic biology tools, employing microorganisms that can remove heavy metal contaminants and use these harvested metals as raw materials to produce valuable nanomaterials for use in medicine and industry. Using a modular approach we are identifying and optimising genetic elements to control nanomaterial production, size, shape and homogeneity in bacteria, tailoring the nanomaterials to their ascribed use, adding value to the decontamination process. One case study we are currently working on is the remediation of arsenic and nickel from contaminated land, using phytoremediation to remove the metals from land before processing the plant material and using our bacterial strains to convert the metals into nanoparticles. We are also working with the whisky industry, recovering copper from the co-products of distillation. O1-6 High cell-density P3HB production in a membrane bioreactor Cornelia Haas 1,, Tarek El-Najjar 2, Nikolaus Virgolini 2, Marina Smerilli 2, Markus Neureiter 2 1 BOKU University of Natural Resources, Austria 2 BOKU University of Natural Resources and Life Sciences, Austria Agro-industrial residues with a high carbon content such as molasses are already intensively used as fermentation substrates. Residues with a lower carbon content, such as whey, stillage,
2 33 S 3 6 July 2016 ISSN , Volume 33, Issue S New Biotechnology Vol. 33/S (2016) S1 S214 ABSTRACTS OF THE 17TH EUROPEAN CONGRESS ON BIOTECHNOLOGY 3 6 JULY 2016, KRAKOW, POLAND BIOTECHNOLOGY Abstract Book ELSEVIER Official Journal of the EFB
3 New BIOTECHNOLOGY Volume 33 /S (2016 ) Amsterdam Boston London New York Oxford Paris Philadelphia San Diego St. Louis
4 Abstracts of the 17th European Congress on Biotechnology 3 6 July 2016 Krakow Poland
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7 NEW BIOTECHNOLOGY Editor-in-Chief: Michael J. Taussig The Babraham Institute, Babraham Research Campus, Cambridge CB22 3AT, UK Consulting Editors: Klaus Ammann University of Bern, Botanic Garden, Switzerland Roland Wohlgemuth Sigma-Aldrich, Buchs, Switzerland Editorial Assistant: Claudia Fonseca Cambridge Protein Arrays Ltd Babraham Research Campus Cambridge CB22 3AT, UK S1 S4 New BIOTECHNOLOGY ABSTRACTS Sunday 3 rd July ACIB Meeting Industrial biotechnology from fundamentals to practice BioTop Meeting Biomolecular technology of proteins Monday 4 th July S6 Plenary Session S6 Environmental biotechnology S8 Gene therapies, stem cell research and tissue engineering S12 Systems and synthetic biology for biocatalysis S16 Functional nano/biomaterials: Production and bioprocessing S21 Mass spectrometry in biotechnology S23 Genomics of cereal crops S24 The microbial cell factory S27 Bioseparations and downstream processing Tuesday 5 th July S30 Plenary Session S30 Bioremediation and biodegradation S32 The mammalian cell factory S34 Metabolic modelling S37 Bioprocessing S42 Glycobiotechnology Systems and synthetic biology for biocatalysis Wednesday 6 th July S45 Plant synthetic biology for food and feed S47 Novel antibody and ligand formats S48 Recombinant protein production S51 Biorefinery for fuels and chemicals S53 Biotechnology for cosmetics: Health and well-being S54 Waste water treatment and bioremediation S56 Biotherapeuticals and vaccines S59 Microbial response to stress S61 Industrial enzymes S64 Nucleic acid technologies S66 Closing Ceremony Highlight & Satellite Events S66 Highlight Event the bioeconomy & biobusiness (Tuesday 5 th July) S67 AFOB-EFB joint event: Cellulosome & beyond Available online at ScienceDirect POSTERS S71 ACIB Meeting Industrial biotechnology from fundamentals to practice S71 BioToP Session Biomolecular technology of protein S73 Biotherapeuticals and vaccines S77 Stem cells and gene therapy S78 Tissue engineering S80 Antimicrobials (continued on next page)
8 S83 Biofuels, biochemicals and bioenergy S96 Biocatalysis and industrial enzyme engineering S110 Bioprocessing and bioengineering S115 Bioseparations and downstream processing S116 Industrial biotechnology S122 Glycobiotechnology S124 Environmental biotechnology S132 Bioremediation and biodegradation S144 Wastewater treatment S146 Nanobiotechnology S150 Polymer biotechnology S154 Plant biotechnology S166 Genomics of cereal crops S167 Nucleic acid technologies S169 Genome editing S171 Molecular tools and bioinformatics S173 Diagnostic tools and biomarkers S175 Genomics S179 Metagenomic applications S180 Proteomics S182 Mass spectrometry in biotechnology S183 Synthetic biology S186 Systems biology S188 Mammalian cell factories S189 Microbial cell factories S195 Microbial response to stress S198 Recombinant protein production and purification S204 Protein and antibody engineering S205 Yeast and filamentous fungi S209 General biotechnology S213 Cellulosome & beyond
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