MagSi Beads. Magnetic Silica Beads for Life Science and Biotechnology study

Transcription

1 MagSi Beads Magnetic Silica Beads for Life Science and Biotechnology study MagnaMedics Diagnostics B.V. / Rev. 9.2 / 2012

2 Wide range of products for numerous applications MagnaMedics separation solutions are based on a proprietary nano- and micro-bead technology incorporating super-paramagnetic properties into a silica matrix. The proven characteristics of silica are also combined with innovative surface modification methods covering a broad range of separation applications. Our standard products have sizes of 600 nm, 1 µm and 1.2 µm. For most applications the 1 µm beads are ideal. Beads with a mean size of 600 nm give more surface area per mg of bead and a slightly slower separation speed. Beads with a mean size of 1.2 µm are used for genomics and proteomics applications where highest magnetic strength and fast protocols are needed. Size Separation speed Surface area 600 nm µm µm MagSi beads are specifically designed for separation, concentration and purification of biomolecules and specific cells or cell compartments. They show excellent performance as a solid phase matrix for manual R&D use, and also serve as platform for automated high throughput applications in laboratory routines. The standard MagSi product portfolio offers scientists enough flexibility towards their personal applications and specified laboratory needs. MagSi-Tools offers a potent tool for flexible and manageable optimization that cannot be achieved with other MagSi products. All MagSi products on offer with regard to e.g. bead size, active surface compounds are outlined in the MagSi Products section underneath. MagSi Products MagSi magnetic beads are designed to meet the specific demands of typical biological applications. We know that scientists have a need for state of the art, innovative products addressing forefront applications. MagSi Advantages - Short separation times - High binding capacity Our magnetic silica beads with different active compounds allow for specific target molecules isolation, purification and concentration out of large and/or complex sample matrix. Where our standard MagSi product portfolio offers solutions for most routine applications; MagSi-Tools beads provide an - Minimal non-specific absorption - Ideal for automated systems - Highly robust - Proven for immunoassay development and downstream applications like PCR - Personal development with MagSi-Tools - Suitable for multiple laboratory routines ideal platform serving your less obvious interests and needs. - Additional protocols for MagSi products available 1 MagnaMedics

3 Bead Application Surface MagSi-Tools - Customer development - Tailored intermediate product for personal applications and pull downs Silica COOH NH 2 MagSi-STA - Isolate biotinylated target molecules - Immunoassay s - Cell/bacteria isolation - Protein purification Streptavidin covalently bound to silica MagSi-protein A MagSi-protein G - Bind IgG antibodies - Antibody purification - Immuno-precipitation from small sample volumes Protein A or Protein G covalently bound to silica MagSi-proteomics - Protein Purification - Concentration and/or desalting of peptide and protein digests C4, C8 or C18 MagSi-WCX MagSi-WAX - Sample preparation - Pre-fractionation prior to mass spectrometry - SDS-PAGE analysis - Protein and peptide separation - Biomarker analysis - Serum/plasma profiling MagSi beads with ion exchange surface (weak cation exchange; weak anion exchange) MagSi beads for DNA isolation - Isolation and purification of nucleic acids Silica COOH - PCR clean-up - Dye Terminator Removal - DNA concentration - Contamination removal Special prepared MagSi-particles including buffers for isolation, purification and concentration of nucleic acid fragments 2 MagnaMedics

4 MagSi-Tools MagSi-Tools are surface activated magnetic particles suited to easily immobilize proteins (e.g. antibodies, enzymes), peptides, nucleic acids or other molecules of interest. Different surface modifications and bead sizes allow for choosing the optimal product for the right molecule to be coupled, and for the intended application. The MagSi-S platform has a range of functionality possibilities such as COOH and NH 2. It is a versatile and adaptable solid phase toolbox for bio-magnetic applications and is ideal for your own specific modification and development. Additional and specific protocols for molecule coupling are available upon request and via our website: MagSi-S mg/ml 600nm MagSi-S mg/ml 1µm MagSi-S COOH mg/ml 600nm MagSi-S COOH mg/ml 1µm MagSi-S NH mg/ml 600nm MagSi-S NH mg/ml 1µm MagSi-STA MagSi-STA beads are useful for many applications, including purification of proteins and peptides or nucleic acids, immuno-precipitation, immuno-assays, protein interaction studies, phage display, and cell isolation. MagSi-STA efficiently binds to biotinylated molecules, e.g. peptides, proteins, antibodies, sugars and lectins. The magnetic properties enable easy and quick washing steps. Because beads are in suspension, incubation times can be shortened compared to alternative techniques. Binding of molecules onto MagSi-STA is based on the strong non-covalent interaction between streptavidin and biotin (K a = M -1 ). MagSi-STA beads are coated with recombinant streptavidin (53 kda) with shortened N- and C-terminus for improved solubility and accessibility of the sites. Albumin binding sites are eliminated for optimal specificity. MagSi-STA mg/ml 600nm MagSi-STA mg/ml 1µm 3 MagnaMedics

5 MagSi-protein A and MagSi-protein G Protein A and Protein G are recombinant proteins that bind with high affinity to the Fc portion of several classes and subclasses of immunoglobulin from a variety of species. Both proteins form reversible complexes with different IgG subclasses and species. MagSi-protein A and MagSi-protein G are ideal tool for efficient and economical purification and immunoprecipitation (IPs) from small sample volumes. Via cross-linking of IgG this product it becomes a tool for directed pull down of desired peptides and proteins. The recombinant protein G (~26kDa) on the surface of our beads contain only IgG binding sites. The albumin, cell wall and cell membrane binding domains, present on the wild-type protein G, have been removed to optimize binding properties. The 42kDa protein A is originating from Staphylococcus aureus. The advantage of MagSi-protein A and MagSi-protein G, compared to MagSi-STA, is that the antibody does not need to be biotinylated and the binding is reversible. The magnetic properties allow easy and quick washing steps, and antibodies are easily crosslinked if interference in downstream processes is expected. This makes the products ideal for protein isolation and proteomics applications. MagSi-protein A mg/ml 600nm MagSi-protein A mg/ml 1µm MagSi-protein G mg/ml 600nm MagSi-protein G mg/ml 1µm MagSi-proteomics The MagSi-proteomics C18 beads are an ideal tool for the purification, concentration and desalting of peptides and protein digests. MagSi-proteomics C8 beads represent an intermediate hydrophobicity (less hydrophobic than C18 and more hydrophobic than C4 beads) and are suitable for sample preparation in context to proteomic profiling and bio-marker research. The relatively low hydrophobicity of the C4 beads allows the purification and fractionation of larger biomolecules like proteins. MagSi-proteomics C4 10mg/ml 1.2µm MagSi-proteomics C8 10mg/ml 1.2µm MagSi-proteomics C18 10mg/ml 1.2µm 4 MagnaMedics

6 MagSi-WCX and MagSi-WAX MagSi-WCX (weak cation exchange) and MagSi-WAX weak anion exchange are ideal tools for the reduction of protein or peptide complexity (e.g. cell lysates). Main applications include: Sample preparation and pre-fractionation prior to mass spectrometry (e.g. MALDI-TOF analysis) SDS-PAGE analysis Protein and peptide separation for multiple downstream applications Bio-marker analysis and serum/plasma profiling The high magnetic strength makes them suitable for both manual and automated/robotic fractionation. A quick and complete separation gives very good reproducibility since no beads will be lost during washing steps. The quick protein adsorption, desorption and magnetic collection significantly shortens the protocol time over conventional column based ion exchange chromatography. MagSi-WAX 20mg/ml 1.2µm MagSi-WCX 20mg/ml 1.2µm Important: Combined use of MagSi-proteomics with e.g. MagSi-WCX, MagSi-WAX or both can provide new details when analyzing your valuable sample. Each of these products focus on different chromatographic features therefore generating more sample information. More information on this topic can be found in the introduction flyers of the MagSi products for proteomics applications. MagSi beads and kits for DNA isolation and purification MagnaMedics offers several distinct magnetic bead types for nucleic acid isolation applications; high throughput applications supported by robotic liquid handling stations typically need magnetic beads with strong magnetic response whereas highly complex, manual DNA extraction applications needs magnetic beads with highest DNA recovery, DNA selectivity and lowest unspecific binding. MagSi beads with a silica surface and a high magnetic content are optimized for nucleic acid isolation from various sources (blood, cells, bacteria etc.) for robotic and manual work flow. Carboxylated MagSi beads guarantee best results in terms of DNA purity and yield. These beads are specially recommended for high DNA yield applications like isolation of viral DNA. MagSi-DNA 300mg/ml 300nm MagSi-DNA allround 20mg/ml 1.2µm MagSi-DNA COOH 300mg/ml 300nm MagSi-DNA allround COOH 20mg/ml 1.2µm 5 MagnaMedics

7 offers a single product solution for PCR cleanup and Dye terminator removal from DNA sequencing reactions, enabling researchers to address various genomic clean-up applications at the same time. The kit can be used for clean-up of different sample types and volumes, is compatible with most consumables and is easily automated for high-throughput processing. Product Volume. 400 / 800 purifications 5,000 / 10,000 purifications MM-Separators MM-separators offer a complete set of magnetic separators suiting researcher's need for manual separation of different sample types, working volumes and throughput. A B MM-Separator M (A) is suitable when larger working volumes (up to 2.0 ml) are required. MM- Separator M96 (B) is intended for sample processing in microtiter plates with multichannel pipettes. The new MM-Separator PCR strip adapter (B, front) is specifically designed for users with smaller sample throughput. The MM-Separator M96 serves as base for the MM-Separator PCR strip adapter, offering a flexible solution for manual handling of your samples. Product Description MM-Separator M MM-Separator M96 MM-Separator PCR strip adapter Magnetic separator for isolation of MagSi beads in 12x 1,5 ml and 12x 2 ml tubes. Magnetic separator for isolation of MagSi beads in microtiterplate format (96 wells) Adapter can be placed on the MM-Separator M96 and is suitable for users with smaller sample throughput, preferring use of PCR tube strips over PCR plates MagnaMedics Diagnostics B.V. Chemelot Campus Burgemeester Lemmensstraat JT Geleen (The Netherlands) Tel: +31-(0) info@magnamedics.com Fax: +31-(0) Web address: MagnaMedics