DNA- THE MOLECULE OF LIFE

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1 DNA- THE MOLECULE OF LIFE

2 STRUCTURE OF DNA DNA (Deoxyribonucleic Acid): DNA is a long, stringy, twisted molecule made up of nucleotides that carries genetic information.

3

4 DISCOVERIES Rosalind Franklin, 1952, was studying the structure of DNA using X-ray diffraction ; helix

5 1953, JAMES WATSON & FRANCIS CRICK Saw Franklin s image and finalized the structure of DNA Called it a double-helix

6 STRUCTURE OF DNA Composition: DNA is composed of nucleotides. The nucleotide is the monomer. Three parts of a nucleotide: 1. 5-Carbon Sugar: Deoxyribose 2. Phosphate Group 3. Nitrogen Base

7 DOUBLE HELIX: WHY? double helix because it consists of two long strands of nucleotides which are twisted together and when untwisted resemble a ladder.

8 The sides of the ladder are made of alternating deoxyribose sugars and phosphate groups held together by covalent bonds. The rungs of the ladder are made up of pairs of nitrogen bases. They follow the base-pairing rule.

9 BASE PAIRING

10 NITROGEN BASES There are four different types of nitrogen bases: Purines: Composed of two rings 1. Adenine (A) 2. Guanine (G) Pyrimidines: Composed of one ring 3. Cytosine (C) 4. Thymine (T) NOTE: 5. Uracil (U) is found only in RNA, NOT in DNA

11 BASE PAIRING RULE: A purine always pairs with a pyrimidine. A = T: Held together with two hydrogen bonds forming a double bond. C = G: Held together with three hydrogen bonds forming a triple bond.

12 BONDS The rungs of the ladder are attached to the sugar part of the side (NOT the phosphate group). The nitrogen bases are held together by weak hydrogen bonds. These weak bonds break apart easily.

13 FUNCTIONS OF DNA:12/2 DNA codes for proteins and hormones to be made. Proteins result in genetic traits. Hormones result in your body functioning The order or sequence of the 4 different nucleotide base pairs and the number of them determines what proteins are made and therefore, what genetic traits are present.

14 DNA REPLICATION Definition: The process of copying DNA Purpose: Gives new cells produced in mitosis and meiosis a complete set of chromosomes. Where Does DNA Replication Occur? Nucleus When Does DNA Replication Occur? The S phase of interphase in the cell cycle

15 STEPS OF REPLICATION: 1. DNA untwists and enzymes break the hydrogen bonds between the nitrogen bases. The DNA unzips.

16 2. Free-floating nucleotides pair with each unzipped strand following the base pairing rule (A with T and C with G). Each strand of DNA acts a template. Hydrogen bonds hold the base pairs together.

17 3. the two new identical molecules of DNA are twisted back up by enzymes. Link to animation of process DNA replication song: Backstreet Boys

18 Result: Two identical copies of DNA each composed of one original and one new strand. Why is replication so important? It occurs during interphase so that each new daughter cell will have a complete set of chromosomes at the end of mitosis.

19 RNA AND TRANSCRIPTION 12/6, 12/7 RNA: Ribonucleic Acid A nucleic acid molecule that is similar to DNA but has different functions. Structure: Composed of RNA nucleotides and is a helix

20 WHAT IS THE DIFFERENCE? RNA differs from DNA in four ways: 1. RNA is single stranded not doubled. 2. Has uracil instead of thymine (still a pyrimidine). 3. Contains the 5-Carbon sugar ribose instead of deoxyribose

21 4. There are 3 types of RNA and only one type of DNA

22 TYPES OF RNA: 1. mrna (messenger): copies instructions in DNA and carries these to the ribosome. The message.

23 TRANSFER RNA trna (transfer): carries amino acids to the ribosome. Contains the anticodon (group of three complementary nitrogen bases to the codon on mrna). Acts as a taxi-cab.

24 RIBOSOMAL RNA rrna (ribosomal): makes up the ribosome. It reads the mrna by grouping mrna together into codons (groups of 3 nitrogen bases.)

25 PROTEIN SYNTHESIS: the process where information in DNA is used to build proteins. Consists of two processes transcription and translation. Transcription: A strand of DNA is copied into a complementary strand of mrna

26 WHY DOES THIS HAPPEN? Why? DNA cannot leave the nucleus because it is too big needs a messenger to carry the information for making proteins to the ribosomes in the cytoplasm. mrna is much smaller because it is single stranded and only a portion of the DNA is copied so it can leave the nucleus. Location: the nucleus of cells

27 PROCESS: 1. DNA is untwisted in only the parts that contain the information needing to be copied. 2. DNA is unzipped by enzymes breaking the hydrogen bonds. 3. Free floating mrna nucleotides pair with one of the unzipped strands of the DNA following the base pairing rule.

28 4. Important Note: Uracil replaces thymine. (G with C, C with G, T with A and A with U). 5. Only one strand of DNA acts as a template. The other just hangs out until the process is over. 6. Enzymes separate the mrna from the DNA template and the mrna enters the cytoplasm and goes to a ribosome.

29 7. The two strands of DNA zip back up, reforming the hydrogen bonds between the nitrogen bases. RESULT: A single strand of mrna is made with information copied from DNA for the building a specific protein. Link to animation

30 TRANSLATION 12/8, 12/9 The process of converting or translating information in a sequence of nitrogen bases in mrna into a sequence of amino acids that make a protein. Before translation can begin, transcription of the DNA into mrna must occur.

31 TRANSLATION : CONTINUED mrna, rrna and trna are all involved 3 steps: mrna attaches to the ribosome (rrna). (The rrna slides along the mrna like a bead on a string.) rrna reads the mrna in groups of three nucleotides called a codon. Translation always begins with a special codon (AUG) called the initiator or start codon.

32 AMINO ACIDS there are 20 different amino acids and 64 different codons. Each amino acid may have more than one codon but each codon specifies for only one amino acid.

33 There are three special stop codons: UAA, UAG and UGA.

34 MRNA CODON CHART

35 MUTATIONS Changes in the genetic material (DNA) Mutations can occur in two different types of cells: 1. Somatic (body) cells 2. Gamete (sex) cells

36 Mutations that occur in somatic (body) cells usually result in killing that body cell only. Mutations that occur in gamete (sex) cells means that every cell of the developing fetus/baby will have that mutation.

37 TYPES OF MUTATIONS IN GAMETE CELLS 2 Types: 1. Gene Mutations 2. Chromosomal Mutations 2 types of Single Gene Mutations: a. Point Mutation: a single point in the DNA sequence is affected.

38 POINT MUTATIONS (CONTINUED) Can be a substitution in which one base is changed into another base. DNA: TAC GCA TGG AAT mrna: AUG CGU ACC UUA Amino Acid:Met - Arg - Thr - Leu DNA: TAC GTA TGG AAT mrna: AUG CAU ACC UUA Amino Acid:Met - His - Thr - Leu

39 2. FRAMESHIFT MUTATION: a single gene or nitrogen base is deleted or added from the mrna sequence causing a shift in the reading frame of the genetic message. Can be an insertion in which one base is inserted in the DNA sequence. Can be a deletion in which one base is deleted in the DNA sequence.

40 FRAMESHIFT MUTATIONS More dramatic affect. may change every amino acid that follows the point of the mutation can alter a protein so much that it is unable to perform its normal functions

41 2 ND TYPE OF MUTATION IN GAMETES 5 types of Chromosomal Mutations: 1. Deletion 2. Duplication 3. Inversion 4. Translocation 5. Non-disjunction

42 DO NOT WRITE: JUST LISTEN! Deletion: Involves the loss of all or part of a chromosome. Duplication: Involves the production of extra copies of parts of the chromosome. Inversion: Reverses the direction of parts of a chromosome. Translocation: When one part of a chromosome breaks off and attaches to another chromosome. Non-disjunction: Means not coming apart. When homologous chromosomes fail to separate properly during meiosis.

43 QUIZ ON CHAPTER 12 : FRIDAY, DEC. 10 th : Blue Classes MONDAY, DEC. 12 th : GOLD CLASSES

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