E.Z.N.A. Size Select-IT Kit. D preps D preps D preps

Save this PDF as:
Size: px
Start display at page:

Download "E.Z.N.A. Size Select-IT Kit. D preps D preps D preps"

Transcription

1 E.Z.N.A. Size Select-IT Kit D preps D preps D preps September 2012

2 E.Z.N.A. Size Select-IT Table of Contents Introduction...2 Illustrated Protocol...3 Size Selection Guide...4 Kit Contents/Storage and Stability...7 Preparing Reagents...8 Recovery of Fragments bp Protocol...9 Recovery of Fragments bp Protocol...12 Recovery of Fragments bp Protocol...15 Recovery of Fragments bp Protocol...18 Troubleshooting...21 Ordering...22 Manual Revision: September 2012 Innovations in nucleic acid isolation 1

3 Introduction Post DNA shearing, the library construction process for next generation sequencing (NGS) requires fragment size selection regardless of the sequencer platform. Obtaining a high recovery rate and a precise DNA size distribution range is key to reduce sequencing bias. The E.Z.N.A. Size Select-IT Kit is optimized for use with the library construction workflows for all the common NGS platforms such as Illumina HiSeq/GA/MiSeq, Roche 454 Genome Sequencer and Life Technologies SOLiD/Ion Torrent. The E.Z.N.A. Size Select-IT Kit utilizes a unique spin column based technology for quick and more optimized DNA size selection suitable for various next generation sequencing platforms. The E.Z.N.A. Size Select-IT Kit is a convenient system for fast and reliable processing of library size selection and purification of enzymatic reactions. The E.Z.N.A. Size Select-IT Kit can selectively and precisely recover DNA fragments between 150 bp to 700 bp free of oligonucleotides, nucleotides, and polymerase with recovery rate exceeding 50%. DNA binding condition is adjusted by adding appropriate volumes of the specially formulated buffers before loading to the column. Following two rapid wash steps, DNA can be eluted with deionized water or a low salt buffer. Benefits of the E.Z.N.A. Size Select-IT Kit Simplicity - No tedious gel purification, no specialize equipment to buy Efficiency - DNA recovery from enzymatic reactions can be achieved in less than 10 minutes Flexibility - Customizable protocol to fine-tune DNA recovery size range Reliability - Optimized buffers that guarantee pure DNA Binding Capacity Each HiBind NGS DNA Mini Columns can bind ~5 µg DNA. New In this Edition The HiBind NGS DNA Mini Columns have been redesigned to increase DNA recovery by reducing the HiBind matrix retention volume. 2

4 Illustrated Protocol Add XE1 Binding Buffer and ACD Buffer Transfer to NGS LS DNA Mini Column Add XE2 Buffer and BCS Buffer to the filtrate Transfer to NGS SS DNA Mini Column Wash 2x Dry Elute 3

5 Size Selection Guide E.Z.N.A. Size Select-IT Kit Size Selection Guides The E.Z.N.A. DNA Size Select-IT Kit offers precise DNA size selection by adding various volumes of ACD Buffer and/or BCS Buffer to the DNA fragment sample. The standard protocols in this booklet are designed for DNA samples dissolved in water or common elution buffers such as Omega Bio-tek s Elution Buffer or Qiagen s EB Buffer. However, DNA size selection range will vary depending on the salt concentration and ph of the DNA sample. If using DNA in buffer other than aforementioned, it is highly advised that one should perform a titration test to confirm the cut-off range of DNA size distribution by using different volumes of ACD Buffer (removal of large DNA fragments) and BCS Buffer (removal of small DNA fragments). Below is a brief outline of the titration test procedure. Please refer to the main manual for procedural details. Removal of Large DNA Fragments The E.Z.N.A. DNA Size Select-IT Kit protocols can be adjusted to fit each user s different DNA fragment size requirements. ACD Buffer selectively removes larger bp fragments from a sheared DNA sample. By increasing the volume of ACD Buffer added to the sheared DNA sample, the larger DNA size cut-off decreases. For example, if DNA is in water or common elution buffer, and fragments less than 400 bp are required, add 8 μl ACD Buffer to the sheared DNA sample (please refer to the table below). 1. Mix 50 µl DNA, 200 µl XE1 Binding Buffer, and various volumes of ACD Buffer (6-14 µl as a guideline. See table below and the Bioanalyzer traces on Page 5 for details.) 2. Pass through the NGS LS DNA Mini Column. 3. To the filtrate add 1 volume XE2 Buffer. 4. Pass through the NGS SS DNA Mini Column. 5. Wash the NGS SS DNA Mini Column twice with DNA Wash Buffer. 6. Spin dry the column. 7. Elute with µl Elution Buffer. 8. Perform gel or Bioanalyzer analysis to determine the optimal volume of ACD Buffer needed for the desired large DNA size cut-off. ACD Buffer 6 μl 8 μl 10 μl 12 μl 14 μl DNA Size Cut-off 500 bp 400 bp 300 bp 250 bp 0 bp 4

6 Size Selection Guide 6 μl ACD Buffer 8 μl ACD Buffer 10 μl ACD Buffer 500 bp 400 bp 300 bp 12 μl ACD Buffer 14 μl ACD Buffer 250 bp Removal of Small DNA Fragments The E.Z.N.A. DNA Size Select-IT Kit protocols can be adjusted to fit each user s different DNA fragment size requirements. BCS Buffer selectively removes smaller bp fragments from a sheared DNA sample. By increasing the volume of BCS Buffer added to the sheared DNA sample, the smaller DNA size cut-off increases. For example, if DNA is in water or common elution buffer, and fragments greater than 150 bp are required, add 20 μl BCS Buffer to the sheared DNA sample (please refer to the table on Page 6). 1. Mix 50 µl DNA, 200 µl XE1 Binding Buffer, and the optimal volume of ACD Buffer as determined in the previous section. 2. Pass through the NGS LS DNA Mini Column. 3. To the filtrate add 1 volume XE2 Buffer and various volumes of BCS Buffer (10-20 µl as a guideline. See table and Bioanalyzer traces on Page 6 for details.). 4. Pass through the NGS SS DNA Mini Column. 5. Wash the NGS SS DNA Mini Column twice with DNA Wash Buffer. 6. Spin dry the column. 7. Elute with µl Elution Buffer. 8. Perform gel or Bioanalyzer analysis to determine the optimal volume of BCS Buffer needed for the desired small DNA size cut-off. 5

7 Size Selection Guide BCS Buffer (μl) Low Cut-off Size Range (bp) ACD Buffer (μl) High Cut-off μl BCS Buffer 6 μl ACD Buffer 12 μl BCS Buffer 6 μl ACD Buffer 14 μl BCS Buffer 6 μl ACD Buffer 100 bp 500 bp 110 bp 500 bp 120 bp 500 bp 16 μl BCS Buffer 6 μl ACD Buffer 18 μl BCS Buffer 6 μl ACD Buffer 20 μl BCS Buffer 6 μl ACD Buffer 130 bp 500 bp 140 bp 500 bp 150 bp 500 bp 6

8 Kit Contents Size Select-IT Kit D D D Preps NGS LS DNA Mini Column NGS SS DNA Mini Column ml Collection Tubes XE1 Binding Buffer 3 ml 12 ml 50 ml XE2 Buffer 3 ml 12 ml 50 ml ACD Buffer 200 μl 1 ml 4 ml BCS Buffer 300 μl 1.5 ml 5 ml DNA Wash Buffer 2 x 1.5 ml 15 ml 2 x 25 ml Elution Buffer 5 ml 5 ml 15 ml User Manual P P P Storage and Stability All E.Z.N.A. Size Select-IT Purification Kit components are guaranteed for at least 12 months from the date of purchase when stored at C. If any precipitates form in buffers, warm at 37 C to dissolve. 7

9 Preparing Reagents Dilute DNA Wash Buffer with 100% ethanol as follows and store at room temperature. Kit Ethanol To Be Added D ml per bottle D ml D ml per bottle 8

10 E.Z.N.A. Size Select-IT Kit Protocol - Recovery of bp Fragments The protocol below is validated using DNA dissolved in water or common elution buffers such as Omega Bio-tek s Elution Buffer or Qiagen s EB Buffer. However, DNA size selection range will vary depending on the salt concentration and ph of the DNA sample. If using DNA in buffer other than aforementioned, or the desired size range is not listed in this manual, it is highly advised that one should perform a titration test to confirm the cut-off range of DNA size distribution by using different volumes of ACD Buffer (removal of large DNA fragments) and BCS Buffer (removal of small DNA fragments). Please refer to the previous section (Size Selection Guide) for titration test procedure. Materials and Equipment to be Supplied by User: Microcentrifuge capable of at least 13,000 x g Nuclease-free 1.5 ml microcentrifuge tubes 100% ethanol Optional: Sterile deionized water or TE Buffer Before Starting: Prepare DNA Wash Buffer according to Preparing Reagents section on Page 8. Prepare sheared DNA and verify the fragments by gel electrophoresis. 1. Add 50 μl DNA fragment dissolved in sterile deionized water or TE Buffer to a nuclease-free 1.5 ml microcentrifuge tube. Note: If less than 50 μl sheared DNA is available, bring the volume up to 50 μl with sterile deionized water or TE Buffer. 2. Add 200 μl XE1 Binding Buffer and 6 μl ACD Buffer. Vortex to mix thoroughly. 3. Centrifuge briefly to collect any droplets from the inside of the lid. 4. Insert a NGS LS DNA Mini Column into a 2 ml Collection Tube. 5. Transfer the sample to the NGS LS DNA Mini Column. 9

11 6. Centrifuge at 13,000 x g for 30 seconds at room temperature. 7. Discard the NGS LS DNA Mini Column and transfer the filtrate to a clean 1.5 ml microcentrifuge tube. 8. Add 1 volume XE2 Buffer (with respect to the filtrate volume). For example, if the filtrate volume is 200 µl, add 200 µl XE2 Buffer. 9. Add 20 µl BCS Buffer. Vortex to mix thoroughly. 10. Centrifuge briefly to collect any droplets from the inside of the lid. 11. Insert a NGS SS DNA Mini Column into a 2 ml Collection Tube. 12. Transfer the sample to the NGS SS DNA Mini Column. 13. Centrifuge at 13,000 x g for 30 seconds at room temperature. 14. Discard the filtrate and reuse the collection tube. 15. Add 700 µl DNA Wash Buffer. Note: DNA Wash Buffer must be diluted with ethanol prior to use. Please see Page 8 for instructions. 16. Centrifuge at 13,000 x g for 30 seconds. 17. Discard the filtrate and reuse the collection tube. 18. Add 500 µl DNA Wash Buffer. 19. Centrifuge at 13,000 x g for 30 seconds. 10

12 20. Discard the filtrate and reuse the collection tube. 21. Centrifuge the empty NGS SS DNA Mini Column for 2 minutes at maximum speed ( 13,000 x g) to dry the column matrix. Note: It is important to dry the column membrane before elution. Residual ethanol may interfere with downstream applications. 22. Transfer the NGS SS DNA Mini Column into a clean 1.5 ml microcentrifuge tube. 23. Add µl Elution Buffer, sterile deionized water, or TE Buffer directly onto the center of column matrix. 24. Let it sit at room temperature for 2 minutes. 25. Centrifuge at 13,000 x g for 1 minute. 26. Store eluted DNA at -20 C. 11

13 E.Z.N.A. Size Select-IT Kit Protocol - Recovery of bp Fragments The protocol below is validated using DNA dissolved in water or common elution buffers such as Omega Bio-tek s Elution Buffer or Qiagen s EB Buffer. However, DNA size selection range will vary depending on the salt concentration and ph of the DNA sample. If using DNA in buffer other than aforementioned, or the desired size range is not listed in this manual, it is highly advised that one should perform a titration test to confirm the cut-off range of DNA size distribution by using different volumes of ACD Buffer (removal of large DNA fragments) and BCS Buffer (removal of small DNA fragments). Please refer to the previous section (Size Selection Guide) for titration test procedure. Materials and Equipment to be Supplied by User: Microcentrifuge capable of at least 13,000 x g Nuclease-free 1.5 ml microcentrifuge tubes 100% ethanol Optional: Sterile deionized water or TE Buffer Before Starting: Prepare DNA Wash Buffer according to Preparing Reagents section on Page 8. Prepare sheared DNA and verify the fragments by gel electrophoresis. 1. Add 50 μl DNA fragment dissolved in sterile deionized water or TE Buffer to a nuclease-free 1.5 ml microcentrifuge tube. Note: If less than 50 μl sheared DNA is available, bring the volume up to 50 μl with sterile deionized water or TE Buffer. 2. Add 200 μl XE1 Binding Buffer and 8 μl ACD Buffer. Vortex to mix thoroughly. 3. Centrifuge briefly to collect any droplets from the inside of the lid. 4. Insert a NGS LS DNA Mini Column into a 2 ml Collection Tube. 12

14 5. Transfer the sample to the NGS LS DNA Mini Column. 6. Centrifuge at 13,000 x g for 30 seconds at room temperature. 7. Discard the NGS LS DNA Mini Column and transfer the filtrate to a clean 1.5 ml microcentrifuge tube. 8. Add 1 volume XE2 Buffer (with respect to the filtrate volume). For example, if the filtrate volume is 200 µl, add 200 µl XE2 Buffer. 9. Add 20 µl BCS Buffer. Vortex to mix thoroughly. 10. Centrifuge briefly to collect any droplets from the inside of the lid. 11. Insert a NGS SS DNA Mini Column into a 2 ml Collection Tube. 12. Transfer the sample to the NGS SS DNA Mini Column. 13. Centrifuge at 13,000 x g for 30 seconds at room temperature. 14. Discard the filtrate and reuse the collection tube. 15. Add 700 µl DNA Wash Buffer. Note: DNA Wash Buffer must be diluted with ethanol prior to use. Please see Page 8 for instructions. 16. Centrifuge at 13,000 x g for 30 seconds. 17. Discard the filtrate and reuse the collection tube. 18. Add 500 µl DNA Wash Buffer. 13

15 19. Centrifuge at 13,000 x g for 30 seconds. 20. Discard the filtrate and reuse the collection tube. 21. Centrifuge the empty NGS SS DNA Mini Column for 2 minutes at maximum speed ( 13,000 x g) to dry the column matrix. Note: It is important to dry the column membrane before elution. Residual ethanol may interfere with downstream applications. 22. Transfer the NGS SS DNA Mini Column into a clean 1.5 ml microcentrifuge tube. 23. Add µl Elution Buffer, sterile deionized water, or TE Buffer directly onto the center of column matrix. 24. Let it sit at room temperature for 2 minutes. 25. Centrifuge at 13,000 x g for 1 minute. 26. Store eluted DNA at -20 C. 14

16 E.Z.N.A. Size Select-IT Kit Protocol - Recovery of bp Fragments The protocol below is validated using DNA dissolved in water or common elution buffers such as Omega Bio-tek s Elution Buffer or Qiagen s EB Buffer. However, DNA size selection range will vary depending on the salt concentration and ph of the DNA sample. If using DNA in buffer other than aforementioned, or the desired size range is not listed in this manual, it is highly advised that one should perform a titration test to confirm the cut-off range of DNA size distribution by using different volumes of ACD Buffer (removal of large DNA fragments) and BCS Buffer (removal of small DNA fragments). Please refer to the previous section (Size Selection Guide) for titration test procedure. Materials and Equipment to be Supplied by User: Microcentrifuge capable of at least 13,000 x g Nuclease-free 1.5 ml microcentrifuge tubes 100% ethanol Optional: Sterile deionized water or TE Buffer Before Starting: Prepare DNA Wash Buffer according to Preparing Reagents section on Page 8. Prepare sheared DNA and verify the fragments by gel electrophoresis. 1. Add 50 μl DNA fragment dissolved in sterile deionized water or TE Buffer to a nuclease-free 1.5 ml microcentrifuge tube. Note: If less than 50 μl sheared DNA is available, bring the volume up to 50 μl with sterile deionized water or TE Buffer. 2. Add 200 μl XE1 Binding Buffer and 10 μl ACD Buffer. Vortex to mix thoroughly. 3. Centrifuge briefly to collect any droplets from the inside of the lid. 4. Insert a NGS LS DNA Mini Column into a 2 ml Collection Tube. 15

17 5. Transfer the sample to the NGS LS DNA Mini Column. 6. Centrifuge at 13,000 x g for 30 seconds at room temperature. 7. Discard the NGS LS DNA Mini Column and transfer the filtrate to a clean 1.5 ml microcentrifuge tube. 8. Add 1 volume XE2 Buffer (with respect to the filtrate volume). For example, if the filtrate volume is 200 µl, add 200 µl XE2 Buffer. 9. Add 20 µl BCS Buffer. Vortex to mix thoroughly. 10. Centrifuge briefly to collect any droplets from the inside of the lid. 11. Insert a NGS SS DNA Mini Column into a 2 ml Collection Tube. 12. Transfer the sample to the NGS SS DNA Mini Column. 13. Centrifuge at 13,000 x g for 30 seconds at room temperature. 14. Discard the filtrate and reuse the collection tube. 15. Add 700 µl DNA Wash Buffer. Note: DNA Wash Buffer must be diluted with ethanol prior to use. Please see Page 8 for instructions. 16. Centrifuge at 13,000 x g for 30 seconds. 17. Discard the filtrate and reuse the collection tube. 18. Add 500 µl DNA Wash Buffer. 16

18 19. Centrifuge at 13,000 x g for 30 seconds. 20. Discard the filtrate and reuse the collection tube. 21. Centrifuge the empty NGS SS DNA Mini Column for 2 minutes at maximum speed ( 13,000 x g) to dry the column matrix. Note: It is important to dry the column membrane before elution. Residual ethanol may interfere with downstream applications. 22. Transfer the NGS SS DNA Mini Column into a clean 1.5 ml microcentrifuge tube. 23. Add µl Elution Buffer, sterile deionized water, or TE Buffer directly onto the center of column matrix. 24. Let it sit at room temperature for 2 minutes. 25. Centrifuge at 13,000 x g for 1 minute. 26. Store eluted DNA at -20 C. 17

19 E.Z.N.A. Size Select-IT Kit Protocol - Recovery of bp Fragments The protocol below is validated using DNA dissolved in water or common elution buffers such as Omega Bio-tek s Elution Buffer or Qiagen s EB Buffer. However, DNA size selection range will vary depending on the salt concentration and ph of the DNA sample. If using DNA in buffer other than aforementioned, or the desired size range is not listed in this manual, it is highly advised that one should perform a titration test to confirm the cut-off range of DNA size distribution by using different volumes of ACD Buffer (removal of large DNA fragments) and BCS Buffer (removal of small DNA fragments). Please refer to the previous section (Size Selection Guide) for titration test procedure. Materials and Equipment to be Supplied by User: Microcentrifuge capable of at least 13,000 x g Nuclease-free 1.5 ml microcentrifuge tubes 100% ethanol Optional: Sterile deionized water or TE Buffer Before Starting: Prepare DNA Wash Buffer according to Preparing Reagents section on Page 8. Prepare sheared DNA and verify the fragments by gel electrophoresis. 1. Add 50 μl DNA fragment dissolved in sterile deionized water or TE Buffer to a nuclease-free 1.5 ml microcentrifuge tube. Note: If less than 50 μl sheared DNA is available, bring the volume up to 50 μl with sterile deionized water or TE Buffer. 2. Add 200 μl XE1 Binding Buffer and 3 μl ACD Buffer. Vortex to mix thoroughly. 3. Centrifuge briefly to collect any droplets from the inside of the lid. 4. Insert a NGS LS DNA Mini Column into a 2 ml Collection Tube. 18

20 5. Transfer the sample to the NGS LS DNA Mini Column. 6. Centrifuge at 13,000 x g for 30 seconds at room temperature. 7. Discard the NGS LS DNA Mini Column and transfer the filtrate to a clean 1.5 ml microcentrifuge tube. 8. Add 1 volume XE2 Buffer (with respect to the filtrate volume). For example, if the filtrate volume is 200 µl, add 200 µl XE2 Buffer. 9. Add 20 µl BCS Buffer. Vortex to mix thoroughly. 10. Centrifuge briefly to collect any droplets from the inside of the lid. 11. Insert a NGS SS DNA Mini Column into a 2 ml Collection Tube. 12. Transfer the sample to the NGS SS DNA Mini Column. 13. Centrifuge at 13,000 x g for 30 seconds at room temperature. 14. Discard the filtrate and reuse the collection tube. 15. Add 700 µl DNA Wash Buffer. Note: DNA Wash Buffer must be diluted with ethanol prior to use. Please see Page 8 for instructions. 16. Centrifuge at 13,000 x g for 30 seconds. 17. Discard the filtrate and reuse the collection tube. 18. Add 500 µl DNA Wash Buffer. 19

21 19. Centrifuge at 13,000 x g for 30 seconds. 20. Discard the filtrate and reuse the collection tube. 21. Centrifuge the empty NGS SS DNA Mini Column for 2 minutes at maximum speed ( 13,000 x g) to dry the column matrix. Note: It is important to dry the column membrane before elution. Residual ethanol may interfere with downstream applications. 22. Transfer the NGS SS DNA Mini Column into a clean 1.5 ml microcentrifuge tube. 23. Add µl Elution Buffer, sterile deionized water, or TE Buffer directly onto the center of column matrix. 24. Let it sit at room temperature for 2 minutes. 25. Centrifuge at 13,000 x g for 1 minute. 26. Store eluted DNA at -20 C. 20

22 Troubleshooting Guide Please use this guide to troubleshot any problems that may arise. For further assistance, please contact the technical support staff, toll free, at Possible Problems and Suggestions Low DNA yields Elution Using Water: Water ph is too low (< 7.5 ) Check the ph of the water, adjust the ph of the water to 8.0 using Tris-HCl (2M, ph 8.5) No DNA eluted DNA Wash Buffer has not been diluted with 100% ethanol Prepare DNA Wash Buffer as instructed on the bottle, or refer to the Preparing Reagents section on Page 8. Optical densities do not agree with DNA yield on agarose gel Trace contaminants eluted from column increase A260. Make sure to wash column as instructed in Steps 15 and 19 of any protocol, rely on agarose gel/ethidium bromide electrophoresis for quantization. DNA sample floats out of well while loading agarose gel Ethanol not completely removed from column. Centrifuge the empty as instructed in Step 21 of any protocol. 21

23 Ordering Information The following components are available for purchase separately. (Call Toll-free at ) Product Part Number Elution Buffer, 100 ml PDR048 DNA Wash Buffer, 100 ml PS010 2 ml Collection Tubes SS ml DNase/RNase-free Microcentrifuge Tubes SS HiBind, E.Z.N.A., and MicroElute are registered trademarks of Omega Bio-tek, Inc. PCR is a patented process of Hoffman-La Roche. Use of the PCR process requires a license. 22

E.Z.N.A. Cycle Pure Kit

E.Z.N.A. Cycle Pure Kit E.Z.N.A. Cycle Pure Kit D6492-00 5 preps V-spin D6492-01 50 preps V-spin D6492-02 200 preps V-spin D6493-00 5 preps Q-spin D6493-01 50 preps Q-spin D6493-02 200 preps Q-spin March 2017 E.Z.N.A. Cycle Pure

More information

E.Z.N.A. Cycle Pure Kit

E.Z.N.A. Cycle Pure Kit E.Z.N.A. Cycle Pure Kit D6492-00 5 preps V-spin D6492-01 50 preps V-spin D6492-02 200 preps V-spin D6493-00 5 preps Q-spin D6493-01 50 preps Q-spin D6493-02 200 preps Q-spin March 2017 E.Z.N.A. Cycle

More information

E.Z.N.A. Gel Extraction Kit

E.Z.N.A. Gel Extraction Kit E.Z.N.A. Gel Extraction Kit D2500-00 5 preps D2500-01 50 preps D2500-02 200 preps D2501-00 5 preps D2501-01 50 preps D2501-02 200 preps Manual Date: November 2018 Revision Number: v3.1 E.Z.N.A. Gel Extraction

More information

MicroElute Cycle-Pure Kit

MicroElute Cycle-Pure Kit MicroElute Cycle-Pure Kit D6293-00 5 preps D6293-01 50 preps D6293-02 200 preps MicroElute Gel Extraction Kit D6294-00 5 preps D6294-01 50 preps D6294-02 200 preps MicroElute DNA Clean Up Kit D6296-00

More information

E.Z.N.A. M13 DNA Mini Kit

E.Z.N.A. M13 DNA Mini Kit E.Z.N.A. M13 DNA Mini Kit D6900-00 5 preps D6900-01 50 preps EZ-96 M13 DNA Kit D1900-00 2 preps D1900-01 6 preps June 2013 E.Z.N.A. M13 DNA Mini Kit E-Z 96 M13 DNA Kit Table of Contents Introduction and

More information

E.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps

E.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps E.Z.N.A. Yeast Plasmid Mini Kit D3376-00 5 preps D3376-01 50 preps November 2015 E.Z.N.A. Yeast Plasmid Mini Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. Insect DNA Kit. D preps D preps D preps

E.Z.N.A. Insect DNA Kit. D preps D preps D preps E.Z.N.A. Insect DNA Kit D0926-00 5 preps D0926-01 50 preps D0926-02 200 preps May 2017 E.Z.N.A. Insect DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. Soil DNA Kit. D preps D preps D preps

E.Z.N.A. Soil DNA Kit. D preps D preps D preps E.Z.N.A. Soil DNA Kit D5625-00 5 preps D5625-01 50 preps D5625-02 200 preps April 2013 E.Z.N.A. Soil DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. MicroElute Genomic DNA Kit. D preps D preps D preps

E.Z.N.A. MicroElute Genomic DNA Kit. D preps D preps D preps E.Z.N.A. MicroElute Genomic DNA Kit D3096-00 5 preps D3096-01 50 preps D3096-02 200 preps December 2013 E.Z.N.A. MicroElute Genomic DNA Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3

More information

E.Z.N.A. Water DNA Kit. D preps D preps D preps

E.Z.N.A. Water DNA Kit. D preps D preps D preps E.Z.N.A. Water DNA Kit D5525-00 5 preps D5525-01 50 preps D5525-02 200 preps April 2017 E.Z.N.A. Water DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. Blood DNA Mini Kit. D preps D preps D preps

E.Z.N.A. Blood DNA Mini Kit. D preps D preps D preps E.Z.N.A. Blood DNA Mini Kit D3392-00 5 preps D3392-01 50 preps D3392-02 200 preps January 2017 E.Z.N.A. Blood DNA Mini Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage

More information

E.Z.N.A. Soil DNA Kit. D preps D preps D preps

E.Z.N.A. Soil DNA Kit. D preps D preps D preps E.Z.N.A. Soil DNA Kit D5625-00 5 preps D5625-01 50 preps D5625-02 200 preps December 2016 E.Z.N.A. Soil DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. FFPE RNA Kit. R preps R preps R preps

E.Z.N.A. FFPE RNA Kit. R preps R preps R preps E.Z.N.A. FFPE RNA Kit R6954-00 5 preps R6954-01 50 preps R6954-02 200 preps January 2015 E.Z.N.A. FFPE RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. Bacterial DNA Kit. D preps D preps D preps

E.Z.N.A. Bacterial DNA Kit. D preps D preps D preps E.Z.N.A. Bacterial DNA Kit D3350-00 5 preps D3350-01 50 preps D3350-02 200 preps April 2017 E.Z.N.A. Bacterial DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability....3

More information

E.Z.N.A. Bacterial DNA Kit. D preps D preps D preps

E.Z.N.A. Bacterial DNA Kit. D preps D preps D preps E.Z.N.A. Bacterial DNA Kit D3350-00 5 preps D3350-01 50 preps D3350-02 200 preps April 2017 E.Z.N.A. Bacterial DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability....3

More information

E.Z.N.A. Mollusc DNA Kit. D preps D preps D preps

E.Z.N.A. Mollusc DNA Kit. D preps D preps D preps E.Z.N.A. Mollusc DNA Kit D3373-00 5 preps D3373-01 50 preps D3373-02 200 preps May 2017 E.Z.N.A. Mollusc DNA Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Determination

More information

E.Z.N.A. Stool DNA Kit. D preps D preps D preps

E.Z.N.A. Stool DNA Kit. D preps D preps D preps E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps April 2013 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage

More information

E.Z.N.A. Stool DNA Kit. D preps D preps D preps

E.Z.N.A. Stool DNA Kit. D preps D preps D preps E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps July 2017 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage

More information

E.Z.N.A. Yeast RNA Kit. R preps R preps

E.Z.N.A. Yeast RNA Kit. R preps R preps R6870-00 5 preps R6870-01 50 preps September 2017 Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4 Preparing Reagents...5 Yeast RNA Protocol...6

More information

E.Z.N.A. Universal Pathogen Kit. D preps D preps

E.Z.N.A. Universal Pathogen Kit. D preps D preps E.Z.N.A. Universal Pathogen Kit D4035-00 5 preps D4035-01 50 preps March 2016 E.Z.N.A. Universal Pathogen Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. Yeast DNA Kit. D preps D preps

E.Z.N.A. Yeast DNA Kit. D preps D preps E.Z.N.A. Yeast DNA Kit D3370-00 5 preps D3370-01 50 preps May 2017 E.Z.N.A. Yeast DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Recommended

More information

E.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps

E.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps E.Z.N.A. HP Viral RNA/DNA Kit R6873-00 5 preps R6873-01 50 preps August 2013 E.Z.N.A. HP Viral RNA/DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Mag-Bind Total Pure NGS. M ml M ml M ml

Mag-Bind Total Pure NGS. M ml M ml M ml Mag-Bind Total Pure NGS M1378-00 5 ml M1378-01 50 ml M1378-02 500 ml November 2017 Mag-Bind Total Pure NGS Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents and Preparations...4

More information

E.Z.N.A. Bacterial RNA Kit. R preps R preps

E.Z.N.A. Bacterial RNA Kit. R preps R preps E.Z.N.A. Bacterial RNA Kit R6950-00 5 preps R6950-01 50 preps July 2017 E.Z.N.A. Bacterial RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4

More information

Mag-Bind Total Pure NGS. M ml M ml M ml

Mag-Bind Total Pure NGS. M ml M ml M ml Mag-Bind Total Pure NGS M1378-00 5 ml M1378-01 50 ml M1378-02 500 ml August 2016 Mag-Bind Total Pure NGS Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents and Preparations...4

More information

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps E.Z.N.A. Blood DNA Maxi Kit D2492-00 2 preps D2492-03 50 preps May 2013 E.Z.N.A. Blood DNA Maxi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps E.Z.N.A. Blood DNA Maxi Kit D2492-00 2 preps D2492-03 50 preps April 2014 E.Z.N.A. Blood DNA Maxi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

E.Z.N.A. Tissue DNA Kit. D preps D preps D preps

E.Z.N.A. Tissue DNA Kit. D preps D preps D preps E.Z.N.A. Tissue DNA Kit D3396-00 5 preps D3396-01 50 preps D3396-02 200 preps August 2016 E.Z.N.A. Tissue DNA Kit Table of Contents Introduction and Overview...2 Yield and Quality of DNA...3 Illustrated

More information

E.Z.N.A. Blood DNA Midi Kit. D preps D preps

E.Z.N.A. Blood DNA Midi Kit. D preps D preps E.Z.N.A. Blood DNA Midi Kit D3494-00 2 preps D3494-04 100 preps August 2013 E.Z.N.A. Blood DNA Midi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. PF Micro RNA Kit. R preps R preps

E.Z.N.A. PF Micro RNA Kit. R preps R preps E.Z.N.A. PF Micro RNA Kit R7036-00 5 preps R7036-01 50 preps June 2013 E.Z.N.A. PF Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

Mag-Bind RXNPure Plus. M ml M ml M ml

Mag-Bind RXNPure Plus. M ml M ml M ml Mag-Bind RXNPure Plus M1386-00 5 ml M1386-01 50 ml M1386-02 500 ml May 2014 Mag-Bind RXNPure Plus Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents and Preparations...4

More information

E.Z.N.A. DNA/RNA Kit. R preps R preps

E.Z.N.A. DNA/RNA Kit. R preps R preps E.Z.N.A. DNA/RNA Kit R6731-00 5 preps R6731-01 50 preps June 2015 E.Z.N.A. DNA/RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Important

More information

Mag-Bind E-Z Pure. M ml M ml M ml

Mag-Bind E-Z Pure. M ml M ml M ml Mag-Bind E-Z Pure M1380-00 5 ml M1380-01 50 ml M1380-02 500 ml January 2013 Mag-Bind E-Z Pure Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents and Preparations...4

More information

E.Z.N.A. mirna Kit. R preps R preps R preps

E.Z.N.A. mirna Kit. R preps R preps R preps E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

E.Z.N.A. HP Plasmid DNA Mini Kit I

E.Z.N.A. HP Plasmid DNA Mini Kit I E.Z.N.A. HP Plasmid DNA Mini Kit I D7043-00 5 preps V - Spin D7043-01 50 preps V - Spin E.Z.N.A. HP Plasmid DNA Mini Kit II D7045-00 5 preps V - Spin D7045-02 200 preps V - Spin July 2013 E.Z.N.A. HP

More information

E.Z.N.A. DNA/RNA Kit. R preps R preps. Manual Date: November 2018 Revision Number: v3.0

E.Z.N.A. DNA/RNA Kit. R preps R preps. Manual Date: November 2018 Revision Number: v3.0 E.Z.N.A. DNA/RNA Kit R6731-00 5 preps R6731-01 50 preps Manual Date: November 2018 Revision Number: v3.0 E.Z.N.A. DNA/RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3

More information

E.Z.N.A. Plant Direct PCR Kit

E.Z.N.A. Plant Direct PCR Kit E.Z.N.A. Plant Direct PCR Kit TQ2800-00 TQ2800-01 20 preps 100 preps June 2013 E.Z.N.A. Plant Direct PCR Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Plant

More information

Mag-Bind RX Plasmid 96 Kit. Table of Contents

Mag-Bind RX Plasmid 96 Kit. Table of Contents Mag-Bind RX Plasmid 96 Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4 Mag-Bind RX Plasmid 96 Protocol...5 Troubleshooting Guide...8 Manual Revision:

More information

E.Z.N.A. Plant RNA Kit. R preps R preps R preps

E.Z.N.A. Plant RNA Kit. R preps R preps R preps E.Z.N.A. Plant RNA Kit R6827-00 5 preps R6827-01 50 preps R6827-02 200 preps September 2014 E.Z.N.A. Plant RNA Kit Table of Contents Introduction...2 Illustrated Protocol...3 Kit Contents and Storage...4

More information

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator Catalog No. D4080 Highlights Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double

More information

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection

Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double size selection INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator Catalog No. D4080 Highlights Quick and easy 7 minute protocol to select for 300 bp, 200 bp, 150 bp, 100 bp, 50 bp DNA fragments or perform a double

More information

E.Z.N.A. Plant RNA Kit. R preps R preps R preps

E.Z.N.A. Plant RNA Kit. R preps R preps R preps E.Z.N.A. Plant RNA Kit R6827-00 5 preps R6827-01 50 preps R6827-02 200 preps August 2014 E.Z.N.A. Plant RNA Kit Table of Contents Introduction...2 Illustrated Protocol...3 Kit Contents and Storage...4

More information

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE Introduction The GF-1 PCR Clean Up Kit is a system designed for rapid clean up of DNA bands ranging from 100bp to 20kb. The GF-1 PCR Clean Up Kit contains special buffers to provide the correct salt concentration

More information

Mag-Bind Stool DNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind Stool DNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind Stool DNA 96 Kit M4016-00 1 x 96 preps M4016-01 4 x 96 preps June 2018 Mag-Bind Stool DNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Mag-Bind Stool DNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind Stool DNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind Stool DNA 96 Kit M4016-00 1 x 96 preps M4016-01 4 x 96 preps February 2016 Mag-Bind Stool DNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Mag-Bind Bacterial DNA 96 Kit. M x 96 preps M x 96 preps M x 96 preps

Mag-Bind Bacterial DNA 96 Kit. M x 96 preps M x 96 preps M x 96 preps Mag-Bind Bacterial DNA 96 Kit M2350-00 1 x 96 preps M2350-01 4 x 96 preps M2350-02 20 x 96 preps April 2012 Mag-Bind Bacterial DNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage

More information

E.Z.N.A. BAC/PAC Maxi Kit. D preps D preps

E.Z.N.A. BAC/PAC Maxi Kit. D preps D preps E.Z.N.A. BAC/PAC Maxi Kit D2154-00 2 preps D2154-01 5 preps April 2013 E.Z.N.A. BAC/PAC Maxi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

E.Z.N.A. Tissue RNA Kit. R preps R preps

E.Z.N.A. Tissue RNA Kit. R preps R preps E.Z.N.A. Tissue RNA Kit R6688-00 5 preps R6688-01 50 preps May 2015 E.Z.N.A. Tissue RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Important Notes...4 Homogenization

More information

Mag-Bind Bacterial DNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind Bacterial DNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind Bacterial DNA 96 Kit M2350-00 1 x 96 preps M2350-01 4 x 96 preps September 2017 Mag-Bind Bacterial DNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3

More information

Thermo Scientific GeneJET NGS Cleanup Kit

Thermo Scientific GeneJET NGS Cleanup Kit PRODUCT INFORMATION Thermo Scientific GeneJET NGS Cleanup Kit #K0851, #K0852 www.thermoscientific.com/onebio #K0851, K0852 Lot Expiry Date _ CERTIFICATE OF ANALYSIS Thermo Scientific GeneJET NGS Cleanup

More information

Plus DNA Clean/Extraction Kit

Plus DNA Clean/Extraction Kit Plus DNA Clean/Extraction Kit Cat. # : DP034P/ DP034P-300 Size : 50/300 Reactions Store at RT For research use only 1 Description: The Plus DNA Clean/Extraction Kit is designed to extract DNA fragments

More information

E.Z.N.A. Blood RNA Kit. R preps R preps

E.Z.N.A. Blood RNA Kit. R preps R preps E.Z.N.A. Blood RNA Kit R6814-00 5 preps R6814-01 50 preps June 2013 E.Z.N.A. Blood RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS

INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS ERRORE. IL SEGNALIBRO NON È DEFINITO. IMPORTANT NOTES 1 METHOD FOR DNA EXTRACTION FROM AGAROSE GELS 2 METHOD FOR DNA EXTRACTION FROM

More information

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I E.Z.N.A. Endo-free Plasmid DNA Mini Kit I D6948-00 5 preps D6948-01 50 preps D6948-02 200 preps E.Z.N.A. Endo-free Plasmid DNA Mini Kit II D6950-00 5 preps D6950-01 50 preps D6950-02 200 preps November

More information

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I

E.Z.N.A. Endo-free Plasmid DNA Mini Kit I E.Z.N.A. Endo-free Plasmid DNA Mini Kit I D6948-00 5 preps D6948-01 50 preps D6948-02 200 preps E.Z.N.A. Endo-free Plasmid DNA Mini Kit II D6950-00 5 preps D6950-01 50 preps D6950-02 200 preps May 2013

More information

MicroElute Total RNA Kit. R preps R preps R preps

MicroElute Total RNA Kit. R preps R preps R preps MicroElute Total RNA Kit R6831-00 5 preps R6831-01 50 preps R6831-02 200 preps February 2014 MicroElute Total RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3

More information

BIO BASIC. PCR Purification Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only

BIO BASIC. PCR Purification Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only BIO BASIC PCR Purification Kit 9K-006-0003s (10 preps) 9K-006-0003 (100 preps) 9K-006-0004 (200 preps) For Research Use Only Index PCR Purification Kit Introduction 3 Important Notes 4 General Protocol

More information

E.Z.N.A. Forensic DNA Kit. D preps D preps D preps

E.Z.N.A. Forensic DNA Kit. D preps D preps D preps E.Z.N.A. Forensic DNA Kit D3591-00 5 preps D3591-01 50 preps D3591-02 200 preps January 2016 E.Z.N.A. Forensic DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3

More information

Mag-Bind Plant DNA Plus Kit. M x 96 preps M x 96 preps

Mag-Bind Plant DNA Plus Kit. M x 96 preps M x 96 preps Mag-Bind Plant DNA Plus Kit M1128-00 1 x 96 preps M1128-01 4 x 96 preps December 2013 Mag-Bind Plant DNA Plus Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

Mag-Bind PX Blood RNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind PX Blood RNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind PX Blood RNA 96 Kit M7763-00 1 x 96 preps M7763-01 4 x 96 preps June 2018 Mag-Bind PX Blood RNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

TIANgel Mini DNA Purification Kit

TIANgel Mini DNA Purification Kit TIANgel Mini DNA Purification Kit For DNA purification from agarose and polyacrylamide gels www.tiangen.com/en DP130419 TIANgel Mini DNA Purification Kit Kit Contents (Spin column) Cat. no. DP208 Contents

More information

E.Z.N.A. Total RNA Kit II. R preps R preps R preps

E.Z.N.A. Total RNA Kit II. R preps R preps R preps E.Z.N.A. Total RNA Kit II R6934-00 5 preps R6934-01 50 preps R6934-02 200 preps September 2015 E.Z.N.A. Total RNA Kit II Table of Contents Introduction...2 Illustrated Protocol...3 Kit Contents/Storage

More information

Mag-Bind Plant DNA Plus Kit. M x 96 preps M x 96 preps

Mag-Bind Plant DNA Plus Kit. M x 96 preps M x 96 preps Mag-Bind Plant DNA Plus Kit M1128-00 1 x 96 preps M1128-01 4 x 96 preps December 2013 Mag-Bind Plant DNA Plus Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

E.Z.N.A. Microorganism Direct PCR Kit

E.Z.N.A. Microorganism Direct PCR Kit E.Z.N.A. Microorganism Direct PCR Kit TQ3100-00 TQ3100-01 TQ3100-02 20 preps 100 preps 500 preps June 2013 E.Z.N.A. Microorganism Direct PCR Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage

More information

Size-Select Kit for NGS Library Preparation Product # 53600

Size-Select Kit for NGS Library Preparation Product # 53600 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Size-Select Kit for NGS Library Preparation Product # 53600 Product

More information

E.Z.N.A. HP Total RNA Kit. R preps R preps R preps

E.Z.N.A. HP Total RNA Kit. R preps R preps R preps E.Z.N.A. HP Total RNA Kit R6812-00 5 preps R6812-01 50 preps R6812-02 200 preps March 2017 E.Z.N.A. HP Total RNA Kit Table of Contents Introduction...2 Illustrated Protocols...3 Kit Contents/Storage and

More information

Gel/PCR Extraction Kit

Gel/PCR Extraction Kit Gel/PCR Extraction Kit Item No: EX-GP200 (200rxns) Content Content Binding Buffer BD Wash Buffer PE Elution Buffer (10 mm Tris-HCl, ph 8.5) Spin Columns EX-GP200 80 ml 20 mlx3 10 ml 200 each Description

More information

Mag-Bind FFPE DNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind FFPE DNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind FFPE DNA 96 Kit M6958-00 1 x 96 preps M6958-01 4 x 96 preps January 2018 Mag-Bind FFPE DNA Kit Mag-Bind FFPE DNA 96 Kit Table of Contents Introduction and Overview...2 Kit Contents...3 Preparing

More information

GenepHlow Gel Extraction Kit

GenepHlow Gel Extraction Kit Instruction Manual Ver. 02.10.17 For Research Use Only GenepHlow Gel Extraction Kit DFG004 (4 Preparation Sample Kit) DFG100 (100 Preparation Kit) DFG300 (300 Preparation Kit) Advantages Convenient: includes

More information

AxyPrep PCR Clean-up Kit AxyPrep-96 PCR Clean-up Kit

AxyPrep PCR Clean-up Kit AxyPrep-96 PCR Clean-up Kit AxyPrep PCR Clean-up Kit AxyPrep-96 PCR Clean-up Kit For the purification of amplicons from PCRs Kit contents, storage and stability AxyPrep PCR Clean-up Kit Cat. No. AP-PCR-4 AP-PCR-50 AP-PCR-250 Kit

More information

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. INSTRUCTION MANUAL ZymoPURE Plasmid Miniprep Kit Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using

More information

E.Z.N.A. Plasmid DNA Mini Kit I

E.Z.N.A. Plasmid DNA Mini Kit I E.Z.N.A. Plasmid DNA Mini Kit I D6942-00 5 preps Q - Spin D6942-01 50 preps Q - Spin D6942-02 200 preps Q - Spin D6943-00 5 preps V - Spin D6943-01 50 preps V - Spin D6943-02 200 preps V - Spin E.Z.N.A.

More information

Updated August well High-multiplexing Tagmentation and Amplification Robyn Tanny August Company Kit Catalog Number.

Updated August well High-multiplexing Tagmentation and Amplification Robyn Tanny August Company Kit Catalog Number. 96-well High-multiplexing Tagmentation and Amplification Robyn Tanny August 2015 This protocol uses the following purchased reagents: Company Kit Catalog Number Illumina Nextera DNA Sample Preparation

More information

E.Z.N.A. Plasmid DNA Mini Kit I

E.Z.N.A. Plasmid DNA Mini Kit I E.Z.N.A. Plasmid DNA Mini Kit I D6942-00 5 preps Q - Spin D6942-01 50 preps Q - Spin D6942-02 200 preps Q - Spin D6943-00 5 preps V - Spin D6943-01 50 preps V - Spin D6943-02 200 preps V - Spin E.Z.N.A.

More information

Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085

Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085 INSTRUCTION MANUAL Select-a-Size DNA Clean & Concentrator MagBead Kit Catalog No. D4084 & D4085 Highlights Tunable: Quick 10 minute protocol for size selection can be tuned from 100 bp to 1000 bp with

More information

BIO BASIC. Gel Extraction Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only

BIO BASIC. Gel Extraction Kit. 9K s (10 preps) 9K (100 preps) 9K (200 preps) For Research Use Only BIO BASIC Gel Extraction Kit 9K-006-0001s (10 preps) 9K-006-0001 (100 preps) 9K-006-0002 (200 preps) For Research Use Only Index Gel Extraction Kit Introduction 3 Important Notes 4 General Protocol 5 Troubleshooting

More information

TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0

TaKaRa MiniBEST Agarose Gel DNA Extraction Kit Ver.4.0 Cat. # 9762 For Research Use TaKaRa MiniBEST Agarose Gel Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Shipping and Storage... 3 IV. Preparation before Use... 4 V. Protocol...

More information

Product # Specifications. Kit Specifications Column Binding Capacity. 10 g Maximum Weight of Gel Slice Average DNA Recovery 70 90%

Product # Specifications. Kit Specifications Column Binding Capacity. 10 g Maximum Weight of Gel Slice Average DNA Recovery 70 90% 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com DNA Gel Extraction Kit Product # 13100 Product Insert The Norgen

More information

Plasmid DNA Extraction Miniprep Kit

Plasmid DNA Extraction Miniprep Kit BIO BASIC Plasmid DNA Extraction Miniprep Kit 9K-006-0009s (10 preps) 9K-006-0009 (100 preps) 9K-006-0010 (200 preps) For Research Use Only Index Plasmid DNA Extraction Miniprep Kit Introduction 3 Important

More information

E.Z.N.A. Total RNA Kit I. R preps R preps R preps

E.Z.N.A. Total RNA Kit I. R preps R preps R preps E.Z.N.A. Total RNA Kit I R6834-00 5 preps R6834-01 50 preps R6834-02 200 preps August 2012 E.Z.N.A. Total RNA Kit I Table of Contents Introduction...2 Protocol Overview...3 Kit Contents/Storage and Stability...4

More information

Gel Extraction & PCR Purification Combo Kit (Spin-column)

Gel Extraction & PCR Purification Combo Kit (Spin-column) Note: for laboratory research use only. Gel Extraction & PCR Purification Combo Kit (Spin-column) Cat#: DP1501 (50 preps) DP1502 (100 preps) DP1503 (200 preps) BioTeke Corporation I. Kit Content Storage

More information

Sequencing Reaction Clean-Up Kit Product # 34500

Sequencing Reaction Clean-Up Kit Product # 34500 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Sequencing Reaction Clean-Up Kit Product # 34500 Product Insert

More information

Storage and Stability

Storage and Stability Contents Introduction....................................................... 2 Kit Contents...................................................... 3 Before Starting....................................................

More information

Gene-Spin DNA Purification Kit - V3

Gene-Spin DNA Purification Kit - V3 Protech Technology 波仕特生物科技 Gene-Spin 1-4-3 DNA Purification Kit - V3 (For PCR/DNA clean-up & Gel Extraction) Innovative Tools for Nucleic Acid Purification This Kit is for research purposes only. Not for

More information

Presto Mini Plasmid Kit

Presto Mini Plasmid Kit Instruction Manual Ver. 03.06.17 For Research Use Only Presto Mini Plasmid Kit PDH004 (4 Preparation Sample Kit) PDH100 (100 Preparation Kit) PDH300 (300 Preparation Kit) Advantages Sample: 1-7 ml of cultured

More information

Large DNA Fragments Extraction Kit

Large DNA Fragments Extraction Kit Instruction Manual Ver. 04.25.17 For Research Use Only Large DNA Fragments Extraction Kit DFL004 (4 Preparation Sample Kit) DFL100 (100 Preparation Kit) DFL300 (300 Preparation Kit) Advantages Efficient:

More information

Micro-Elute DNA Clean/Extraction Kit

Micro-Elute DNA Clean/Extraction Kit Micro-Elute DNA Clean/Extraction Kit (PCR Clean-up/Gel Extraction/DNA Clean-Up /Concentration Kit) Cat. # : DP034ME/ DP034ME-300 Size : 50/300 Reactions Store at RT For research use only 1 Description:

More information

E-Z 96 Tissue DNA Kit

E-Z 96 Tissue DNA Kit E-Z 96 Tissue DNA Kit D1196-00 1 x 96 preps D1196-01 4 x 96 preps D1196-02 20 x 96 preps Manual Date: November 2018 Revision Number: v9.1 E-Z 96 Tissue DNA Kit Table of Contents Introduction and Principle...2

More information

Plasmid DNA Extraction Midiprep Kit

Plasmid DNA Extraction Midiprep Kit BIO BASIC Plasmid DNA Extraction Midiprep Kit 9K-006-0027s (1 prep) 9K-006-0027 (25 preps) Toll Free 1-877-956-7778 ForOrder Research Use Only www.feldan.com 1 Index Plasmid DNA Extraction Midiprep Kit

More information

E.Z.N.A. Plasmid DNA Midi Kit

E.Z.N.A. Plasmid DNA Midi Kit E.Z.N.A. Plasmid DNA Midi Kit D6904-00 2 preps D6904-03 25 preps D6904-04 100 preps E.Z.N.A. Plasmid DNA Maxi Kit D6922-00 2 preps D6922-01 5 preps D6922-02 20 preps D6922-04 100 preps July 2015 E.Z.N.A.

More information

Soil DNA Extraction Kit

Soil DNA Extraction Kit Instruction Manual Ver. 09.23.16 For Research Use Only Soil DNA Extraction Kit Advantages IB47800 (4 Preparation Sample Kit) IB47801 (50 Preparation Kit) IB47802 (100 Preparation Kit) Sample: 250-500 mg

More information

Mag-Bind Plasmid DNA 96 Kit. M x 96 preps M x 96 preps

Mag-Bind Plasmid DNA 96 Kit. M x 96 preps M x 96 preps Mag-Bind Plasmid DNA 96 Kit M1256-00 1 x 96 preps M1256-02 24 x 96 preps September 2013 Mag-Bind Plasmid DNA 96 Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

TIANamp Marine Animals DNA Kit

TIANamp Marine Animals DNA Kit TIANamp Marine Animals DNA Kit For isolation of genomic DNA from marine animal tissues www.tiangen.com/en DP121221 TIANamp Marine Animals DNA Kit (Spin Column) Cat. no. DP324 Kit Contents Contents DP324-02

More information

DNA Visualizer Extraction Kit

DNA Visualizer Extraction Kit DNA Visualizer Extraction Kit Catalog Number D0006 50 reactions Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...

More information

Bacteria Genomic DNA Purification Kit

Bacteria Genomic DNA Purification Kit Bacteria Genomic DNA Purification Kit Cat #:DP025/ DP025-150 Size:50/150 reactions Store at RT For research use only 1 Description: The Bacteria Genomic DNA Purification Kit provides a rapid, simple, and

More information

Presto Soil DNA Extraction Kit

Presto Soil DNA Extraction Kit Instruction Manual Ver. 02.23.17 For Research Use Only Presto Soil DNA Extraction Kit Advantages SLD004 (4 Preparation Sample Kit) SLD050 (50 Preparation Kit) SLD100 (100 Preparation Kit) Sample: 250-500

More information

AxyPrep Blood Genomic DNA Miniprep Kit

AxyPrep Blood Genomic DNA Miniprep Kit AxyPrep Blood Genomic DNA Miniprep Kit For the purification of genomic DNA from whole blood Kit contents, storage and stability Cat. No. AP-MN-BL-GDNA-4 AP-MN-BL-GDNA-50 AP-MN-BL-GDNA-250 Kit size 4 preps

More information

Presto Food DNA Extraction Kit

Presto Food DNA Extraction Kit Instruction Manual Ver. 06.28.17 For Research Use Only Presto Food DNA Extraction Kit Advantages FGD004 (4 Preparation Sample Kit) FGD100 (100 Preparation Kit) FGD300 (300 Preparation Kit) Sample: 200

More information

StrataPrep PCR Purification Kit

StrataPrep PCR Purification Kit StrataPrep PCR Purification Kit INSTRUCTION MANUAL Catalog #400771 and #400773 Revision A For In Vitro Use Only 400771-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this

More information