Fisher BioReagents Buffers for Life Science Research

Size: px
Start display at page:

Download "Fisher BioReagents Buffers for Life Science Research"

Transcription

1 Fisher BioReagents Buffers for Life Science Research

2 The Water Makes a Difference. CellPURE Biological Buffers are of the highest quality (purity 99%). They are tested for low heavy metal content and the absence of endotoxins, nucleases, and proteases. It is important to use only water of the highest quality to prepare the buffer solutions. Water that stands too long in pipes or water produced by a system needing routine maintenance increases the risk for contamination of buffer solutions. Fisher BioReagents provide several grades of high purity water suitable for the preparation of high quality buffer solutions. Molecular grade water Cat. No. Description Pack Size List price Price FSBBP Sterile biotech grade 4L $81.25 $60.95 FSBBP Sterile biotech grade 20L $ $ FSBBP Sterile DEPC treated nuclease free 500mL $45.55 $34.15 FSBBP Sterile DEPC treated nuclease free 100mL $71.90 $53.90 FSBBP DNA Grade 1L $68.00 $51.00 FSBBP561-1 RNA Grade, DEPC-treated 1L $93.95 $

3 Reagents for Life Science Applications Packaged for convenience, our superior quality, value-added products are prequalified and guaranteed to be suitable for your designated technique or application. For higher productivity in your routine research techniques, choose Fisher BioReagents products Contents CellPURE Biological Buffers Application examples: Tissue culture media and maintenance of cell lines Enzyme assays Electrophoresis of RNA Transfection of mammalian cells JustPURE "Good" Buffers Application examples: High efficiency transfection of mammalian cells Gel electrophoresis of RNA Protein isolation applications Cell cultures and enzyme assays Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE Additional Buffers from Fisher BioReagents Application examples: Electrophoresis of nucleic acids and proteins Isolation and purification of biomolecules Cell and tissue culture Guaranteed DNase-, RNase-, and protease-free 3

4 CellPURE Biological Buffers Fisher BioReagents buffers meet the needs of every budget and scale Economical powders come in various package sizes Concentrated stock solutions provide convenience in one step of easy dilution Ready-to-use solutions offer the biggest time savings of all Ultrapure zwitterionic biological buffers Optimised for cell/tissue culture work Wide applicability to molecular biology and biochemical studies Manufactured under strict quality control guidelines to ensure performance and reliabilty Obtaining optimal results in your research requires careful selection of reagents. When your experiments require exact buffering conditions, you can depend on the reliability of Fisher BioReagents buffers. All Fisher BioReagents buffers are manufactured from high-quality raw materials under ISO 9001:2008-certified manufacturing and testing processes. The purpose of a buffer in a biological system is to maintain intracellular and extracellular ph within the physiological range and resist changes in ph due to the presence of internal and external influences. CellPURE Biological Buffers from Fisher BioReagents are ultrapure zwitterionic buffers that possess both positive and negative charges. First described by Good and co-workers in 1966, these organic buffers have pka values at or near physiological ph and minimal interference with biological processes. CellPURE Biological Buffers are ideal for cell cultivation, isolation of cells, enzyme assays, and other biochemical applications. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): ) Advantages Cell culture tested Analysed for the absence of nucleases and proteases Tested for endotoxin and bioburden levels pka values mostly independent of temperature and concentration High water solubility Minimal permeability to biological membranes Applications Tissue culture media and maintenance of cell lines Enzyme assays Electrophoresis of RNA Transfection of mammalian cells CellPURE Buffers Cat. No. Description Pack Size List Price Price FSBBP BIS-TRIS 100g $ $ FSBBP BIS-TRIS propane 100g $ $ FSBBP MOPS 100g $ $ FSBBP MOPS sodium salt 25g $95.13 $76.15 FSBBP HEPES 100g $ $ FSBBP HEPES sodium salt 100g $ $

5 JustPURE Good Buffers Zwitterionic buffers possessing both positive and negative charges were described in 1966 by Good and co-workers as being suitable for work with biological molecules. Popularly known today as Good buffers, these organic buffers have several advantages compared to traditional inorganic buffering systems such as phosphate, borate, and bicarbonate. Good buffers approach the ideal buffer state by having pka values at or near physiological ph and minimal interference with biological processes. JustPURE Good buffers from Fisher BioReagents have very high purity (assay > 99%) and only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content. (Reference: Good, N.E., et al. (1966) Hydrogen Ion Buffers for Biological Research. Biochemistry 5(2): ) Advantages pka values mostly independent of temperature and concentration High water solubility Chemically and enzymatically stable Minimal permeability to biological membranes Limited effect on biochemical reactions Minimal absorption in spectral range 240 to 700nm Applications High efficiency transfection of mammalian cells Gel electrophoresis of RNA Protein isolation applications Cell cultures and enzyme assays Bioanalytical methods such as IEF, 2-D electrophoresis, SDS-PAGE JustPURE Buffers Cat. No. Description Pack Size List Price Price FSBBP MES hydrate 250g $ $ FSBBP ACES 100g $ $ FSBBP PIPES 50g $ $ FSBBP BIS-TRIS propane 25g $ $ FSBBP MOPS 100g $ $ FSBBP HEPES 50g $ $ FSBBP EPPS 100g $ $ FSBBP Tricine 100g $ $ FSBBP Gly-Gly 50g $ $ FSBBP TAPS 100g $ $ FSBBP CHES 100g $ $ FSBBP CAPS 100g $ $

6 Buffer Properties Properties of CellPURE Biological Buffers Cat. No. Product Description Formula Molecular Weight 25º C Useful ph Range Cell Culture Tested Endotoxin Assay Nuclease and Protease Tested Size FSBBP BIS-TRIS C 8 H 19 NO to 7.2 X X X 100g FSBBP BIS-TRIS propane C 11 H 26 N 2 O to 9.5 X X X 100g FSBBP MOPS C 7 H 15 NO 4 S to 7.9 X X X 100g FSBBP MOPS sodium salt C 7 H 14 NNaO 4 S to 7.9 X X X 25g FSBBP HEPES C 8 H 18 N 2 O 4 S to 8.2 X X X 100g FSBBP HEPES Sodium Salt C 8 H 17 N 2 NaO 4 S to 8.2 X X X 100g pka = 9.0 for the second dissociation stage Properties of JustPURE Buffers Cat. No. Product Description CAS No. Formula Molecular Weight 25º C Useful ph Range Form Size FSBBP MES hydrate C 6 H 13 NO 4 S xh 2 O (anhydrous) to 6.7 White powder 250g FSBBP ACES C 4 H 10 N 2 O 4 S to 7.5 White powder 100g FSBBP PIPES C 8 H 18 N 2 O 6 S to 7.5 White powder 50g FSBBP BIS-TRIS propane C 11 H 26 N 2 O to 9.5 White powder 25g FSBBP MOPS C 7 H 15 NO 4 S to 7.9 White powder 100g FSBBP HEPES C 8 H 18 N 2 O 4 S to 8.2 White powder 50g FSBBP EPPS C 9 H 20 N 2 O 4 S to 8.7 White powder 100g FSBBP Tricine 1/04/5704 C 6 H 13 NO to 8.8 White powder 100g FSBBP Gly-Gly C 4 H 8 N 2 O to 8.9 White powder 50g FSBBP TAPS C 7 H 17 NO 6 S to 9.1 White powder 100g FSBBP CHES C 8 H 17 NO 3 S to 10.0 White powder 100g FSBBP CAPS C 9 H 19 NO 3 S to 11.1 White powder 100g pka = 9.0 for the second dissociation stage 6

7 Additional Buffers from Fisher BioReagents Choose Fisher BioReagents Buffers for: QUALITY: tight specifications CONSISTENCY: lot-to-lot uniformity SELECTION: powders, concentrated stock solutions, or ready-to-use liquids PACKAGING: state-of-the-art containers designed for safety and utility CONVENIENCE: pre-qualified for a variety of applications ECONOMY: configurations to meet all budgets SCALE: from bench to batch sizes Suitable for Life Science applications: Electrophoresis of nucleic acids and proteins Isolation and purification of biomolecules Cell and tissue culture Guaranteed DNase-, RNase-, and protease-free Fisher BioReagents buffers meet the needs of every budget and scale. Economical powders come in various package sizes Concentrated stock solutions provide convenience in one step of easy dilution Ready-to-use solutions offer the biggest time savings of all Cat. No. Description Pack Size List price Price FSBBP x Tris-Glycine 1L $93.55 $74.85 FSBBP x Tris-Glycine 4L $ $ FSBBP x Tris-Glycine 1L $ $ FSBBP x Tris-Glycine-SDS 1L $96.50 $77.20 FSBBP x Tris-Glycine-SDS 4L $ $ FSBBP x Tris-Glycine-SDS 1L $94.10 $75.30 FSBBP x Tris-Glycine-SDS 1L $ $99.40 FSBBP % Sodium Dodecyl Sulphate 200mL $76.30 $61.05 FSBBP % Sodium Dodecyl Sulphate 1L $ $ FSBBP % Sodium Dodecyl Sulphate 200mL $98.60 $78.90 FSBBP % Sodium Dodecyl Sulphate 1L $ $ FSBBP Sodium Dodecyl Sulphate Powder 100g $55.85 $44.70 FSBBP Sodium Dodecyl Sulphate Powder 500g $ $98.00 FSBBP166-5 Sodium Dodecyl Sulphate Powder 5kg $1, $1,

8 Buffers for Nucleic Acid Electrophoresis Cat. No. Description Pack Size List price Price FSBBP x Tris-Borate-EDTA 1L $63.80 $51.05 FSBBP x Tris-Borate-EDTA 4L $ $89.80 FSBBP x Tris-Borate-EDTA 20L $ $ FSBBP x Tris-Borate-EDTA 1L $ $95.60 FSBBP x Tris-Borate-EDTA 1L $88.70 $71.00 FSBBP x Tris-Borate-EDTA 4L $ $ FSBBP x Tris-Borate-EDTA 20L $ $ FSBBP x Tris-Borate-EDTA 1L $ $ FSBBP x Tris-Acetate-EDTA 4L $ $89.55 FSBBP x Tris-Acetate-EDTA 20L $ $ FSBBP x Tris-Acetate-EDTA 500mL $58.65 $46.95 FSBBP x Tris-Acetate-EDTA 1L $62.70 $50.20 FSBBP x Tris-Acetate-EDTA 4L $ $ FSBBP x Tris-Acetate-EDTA 20L $ $ FSBBP x Tris-Acetate-EDTA 1L $ $ FSBBP x Tris-Acetate-EDTA 500mL $76.90 $61.55 FSBBP x Tris-Acetate-EDTA 1L $ $ FSBBP x Tris-Acetate-EDTA 4L $ $ FSBBP x Tris-Acetate-EDTA 20L $1, $ FSBBP x Tris-Acetate-EDTA 1L $ $ FSBBP MOPS Buffer Powder 100g $ $97.20 FSBBP MOPS Buffer Powder 500g $ $ FSBBP x MOPS Buffer Solution 500mL $90.25 $72.20 FSBBP x MOPS Buffer Solution 1L $ $ Buffers for Hybridization of Nucleic Acids Cat. No. Description Pack Size List price Price FSBBP x Saline-Sodium Phosphate-EDTA 1L $87.20 $69.80 FSBBP x Saline-Sodium Phosphate-EDTA 4L $ $ FSBBP x Saline-Sodium Phosphate-EDTA 20L $ $ FSBBP x Saline-Sodium Citrate 1L $79.55 $63.65 FSBBP x Saline-Sodium Citrate 4L $ $ FSBBP x Saline-Sodium Citrate 20L $ $

9 Buffers for Life Science Applications Cat. No. Description Pack Size List price Price FSBBP M EDTA, ph mL $59.20 $47.40 FSBBP M EDTA, ph mL $77.75 $62.20 FSBBP M EDTA, ph 8.0 1L $ $95.95 FSBBP M EDTA, ph L $ $ FSBBP M EDTA, DEPC, ph mL $71.35 $57.10 FSBBP M EDTA, DEPC, ph mL $ $84.80 FSBBP M EDTA, DEPC, ph 8.0 1L $ $ FSBBP EDTA Powder 500g $88.90 $71.15 FSBBP M HEPES, ph mL $77.15 $61.75 FSBBP M HEPES, ph mL $ $ FSBBP M HEPES, ph 7.3 1L $ $ FSBBP HEPES Crystals 100g $71.45 $57.20 FSBBP HEPES Crystals 500g $ $ FSBBP310-1 HEPES Crystals 1kg $ $ FSBBP310-5 HEPES Crystals 5kg $2, $1, FSBBP PBS Tablets 100 tablets $ $ FSBBP x PBS 4L $ $99.40 FSBBP x PBS 20L $ $ FSBBP x PBS 500mL $55.40 $44.35 FSBBP x PBS 1L $95.40 $76.35 FSBBP x PBS 4L $ $ FSBBP x PBS 20L $ $ FSBBP x PBS Powder Concentrate 10L $60.75 $48.60 FSBBP x PBS Powder Concentrate 50L $ $ FSBBP PBS Powder with Tween pouches $ $ FSBBP x PBS Powder Concentrate 2 x 1L $44.30 $35.45 FSBBP x Sodium Chloride-Tris-EDTA, ph 8.0 1L $79.75 $63.80 FSBBP x Sodium Chloride-Tris-EDTA, ph 8.0 1L $70.75 $56.60 FSBBP x Tris-Buffered Saline, ph 7.4 1L $ $81.00 FSBBP x Tris-Buffered Saline, ph mL $63.60 $50.90 FSBBP x Tris-Buffered Saline, ph 7.4 1L $ $

10 Buffers for Life Science Applications Cat. No. Description Pack Size List price Price FSBBP x Tris-EDTA, ph mL $46.30 $37.05 FSBBP x Tris-EDTA, ph mL $51.45 $41.20 FSBBP x Tris-EDTA, ph 7.4 1L $72.95 $58.40 FSBBP x Tris-EDTA, ph mL $29.20 $23.40 FSBBP x Tris-EDTA, ph mL $46.40 $37.15 FSBBP x Tris-EDTA, ph 8.0 1L $78.60 $62.90 FSBBP x Tris-EDTA, ph mL $43.50 $34.80 FSBBP x Tris-EDTA, ph mL $51.70 $41.40 FSBBP x Tris-EDTA, ph 7.6 1L $76.15 $60.95 FSBBP x Tris-EDTA, ph 7.4 1L $97.55 $78.05 FSBBP x Tris-EDTA, ph mL $58.05 $46.45 FSBBP x Tris-EDTA, ph mL $78.55 $62.85 FSBBP x Tris-EDTA, ph 7.6 1L $ $83.25 FSBBP x Tris-EDTA, ph 8.0 1L $ $ FSBBP x Tris-EDTA 1L $ $ FSBBP M Tris Buffer 500mL $96.80 $77.45 FSBBP M Tris Buffer 1L $ $ FSBBP M Tris Buffer 100mL $58.35 $46.70 FSBBP M Tris Buffer 500mL $ $81.60 FSBBP M Tris Hydrochloride, ph mL $58.85 $47.10 FSBBP M Tris Hydrochloride, ph mL $96.45 $77.20 FSBBP M Tris Hydrochloride, ph mL $67.75 $54.20 FSBBP M Tris Hydrochloride, ph mL $87.20 $69.80 FSBBP M Tris Hydrochloride, ph mL $54.00 $43.20 FSBBP M Tris Hydrochloride, ph mL $82.10 $65.70 FSBBP Tris Hydrochloride Solid 500g $ $ FSBBP153-1 Tris Hydrochloride Solid 1kg $ $ FSBBP Tris Base 500g $ $ FSBBP152-1 Tris Base 1kg $ $ FSBBP152-5 Tris Base 5kg $ $ FSBBP Tris Base 10kg $1, $1, FSBBP Tris Base 25kg $2, $2,

11 EZ-RUNyou Protein Standards Have tried our EZ-Run Protein Standards? Designed to assist in identifying and characterizing unknown proteins inhighly purified markers ladders provide compact and clear bands polyacrylamide gels andand immunoblots -Prestained standards areand indispensable in monitoring protein separation Highly purified markers ladders provide compact and clear bands -and Prestained transfer standards efficiencyare indispensable in monitoring protein separation and transfer -Reference allow quick quick gel gelprogress progressassessment assessment Referencebands band allows Unstainedstandards standards are are most of of proteins -Unstained most suitable suitablefor forprecise precisesizing sizing proteins - All standards are supplied in loading buffer and are ready to use All standards are supplied in loading buffer and are ready to use kda kda 12% SDS-PAGE 12% SDS-PAGE EZ-RUN Prestained Protein Marker BP and BP EZ-RUN Protein Marker BP and BP ORDERING INFORMATION MW Range No. of Bands Description MW Range No. of Bands Unstained Protein Standards kda kDa kDa kDa kda 50 kda ---72kDa 6 10 kda 4-20% SDS-PAGE 8-16% SDS-PAGE EZ-RUN Rec Protein Ladder BP and BP EZ-RUN Prestained Rec Protein Ladder BP and BP Reference Band Reference Band Source kDa 7 Prestained Protein kda 14 Standards kDa kda kda Source Quantity Unstained protein standards 500µL Native proteins 2 x 500µL Recombinant proteins 500µL Native proteins 2 x 500µL BP BP BP μl BP Recombinant proteins Native proteins 500µL 2 x 500µL Recombinant proteins 500µL 2 x 500µL Prestained Protein Standards 500 μl BP BP x 500 μl BP BP Native proteins 72 kda Quantity Catalog No. Recombinant proteins Catalog No. FSBBP FSBBP FSBBP μl FSBBP x 500 μl FSBBP μl FSBBP x 500 μl FSBBP

12 Cut and Keep Preparation of Polyacrylamide Stacking and Separating Gels Separating Gel (total volume, 15 ml) Final Percentage Acrylamide in Gel Stock solutions (total volume 15 ml) Component 5% 6% 7% 7.50% 8% 9% 10% 12% 13% 15% 30% Acrylamide/0.8%Bis- Acrylamide 2.50 ml 3.00 ml 3.50 ml 3.75 ml 4.00 ml 4.50 ml 5.00 ml 6.00 ml 6.50 ml 7.50 ml 4X Tris Cl, ph ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml 3.75 ml H 2 O 8.60 ml 8.10 ml 7.60 ml 7.35 ml 7.10 ml 6.60 ml 6.10 ml 5.10 ml 4.60 ml 3.60 ml 10% SDS 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 150 μl 10% Ammonium Persulfate 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl 50 μl TEMED 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 10 μl 4% Stacking Gel (total volume, 5mL) Component Volume 30% Acrylamide/0.8%Bis-Acrylamide 0.65 ml 4X Tris Cl, ph ml H 2 O 3.00 ml 10% SDS 50 μl 10% Ammonium Persulfate 25 μl TEMED 5 μl Procedure for Gel Preparation 1. In a 25 ml sidearm flask or tube, mix the given volumes of: - Acrylamide/Bis-Acrylamide solution. - Tris-HCl Buffer - H 2 O 2. Degas under vacuum minutes 3. Add DS solution, Ammonium Persulfate solution and TEMED 4. Swirl gently to mix 5. Use immediately Notes: Volumes incuded here are adequate for a gel of dimensions 0.75 cm x 14 cm x 14 cm Recipes are based on the SDS (denaturing)-continuous buffer system of Laemmli (1970) the % acrylamide selected for the separating gel will depend on the moleular sizes of the proteins being separated. All reagents and solutions used in this protocol must be prepared with distilled deionised water. Store at 4ºC for a maximum 5 days Cut here to save this protocol for later Order Placement: To fax an order, use To phone, call To an order, ordersau@thermofisher.com To order online: thermofisher.com.au Contact Us: For customer service, call For service and calibration, call By , labconsumablesau@thermofisher.com Find out more at thermofisher.com.au/bioreagents *Pricing valid until 30th September All Prices are in Australian dollars and exclude GST. All offers subject to Thermo Fisher standard terms and conditions. Errors and omission exempted. Specifications, terms and pricing are subject to change. Please consult your local sales representative for details Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified

Fisher BioReagents Buffers for Life Science Research

Fisher BioReagents Buffers for Life Science Research Fisher BioReagents Buffers for Life Science Research The Water Makes a Difference. CellPURE Biological Buffers are of the highest quality (purity 99%). They are tested for low heavy metal content and the

More information

Buffers for Life Science Research CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers. Quality. Consistency. Selection.

Buffers for Life Science Research CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers. Quality. Consistency. Selection. Buffers for Life cience Research CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers Quality. Consistency. election. CellPURE Biological Buffers Ultrapure zwitterionic biological buffers

More information

Vital Reagents, Essential Products for Life Science Research

Vital Reagents, Essential Products for Life Science Research Vital Reagents, Essential Products for Life Science Research Bringing Chemistry to Life Find the Perfect Reagents for your Life Science Application Fisher BioReagents offer over 1,000 products dedicated

More information

Buffers for Life Science Research. CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers. Quality. Consistency. Selection.

Buffers for Life Science Research. CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers. Quality. Consistency. Selection. Buffers for Life Science Research CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers Quality. Consistency. Selection. CellPURE Biological Buffers performance and reliabilty Fisher

More information

Protein Electrophoresis EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-PAGE Reagents

Protein Electrophoresis EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-PAGE Reagents Protein Electrophoresis EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-PAGE Reagents Reliability. Purity. Certainty. Introduction Sodium dodecyl sulfate

More information

Nucleic Acid Electrophoresis APPLICATION GUIDE

Nucleic Acid Electrophoresis APPLICATION GUIDE AGAROSE BUFFERS LADDERS EQUIPMENT Nucleic Acid Electrophoresis APPLICATION GUIDE Reagents: Agarose Thermo Scientific and Fisher Scientific products deliver an end-to-end solution that can meet your most

More information

AMRESCO Biochemical and Molecular Biology Reagents

AMRESCO Biochemical and Molecular Biology Reagents AMRESCO Biochemical and Molecular Biology Reagents BIOCHEMICAL AND MOLECULAR BIOLOGY REAGENTS pure Pure precise performance Precise Performance Antibiotics Media & Components Western Blotting & ELISA Nucleic

More information

Electrophoresis. Ready-to-Run Buffers and Solutions

Electrophoresis. Ready-to-Run Buffers and Solutions Electrophoresis Ready-to-Run Buffers and Solutions Bio-Rad is a premier provider of buffers and premixed reagents for life science research. We offer a variety of different products for all your protein

More information

1st BASE Biochemicals, Buffers And Reagents

1st BASE Biochemicals, Buffers And Reagents 1st BASE Biochemicals, Buffers And Reagents Where Reliability and Convenience count in your research work Our products are applicable for use in routine laboratory applications Nucleic Acid Analysis and

More information

Biochemical and molecular biology reagents 01. PURE 02. PRECISE 03. PERFORMANCE

Biochemical and molecular biology reagents 01. PURE 02. PRECISE 03. PERFORMANCE Biochemical and molecular biology reagents 01. PURE 02. PRECISE 03. PERFORMANCE RESEARCH PRODUCTS FOR MOLECULAR BIOLOGY Antibiotics Media & Components Western Blotting & ELISA Nucleic Acid Analysis Buffers

More information

AFFINITY HIS-TAG PURIFICATION

AFFINITY HIS-TAG PURIFICATION DESCRIPTION Test kits are a fast and easy way to screen different IMAC resins before choosing the most appropriate product for each target-protein. All the products supplied in the Test kit are suitable

More information

TURBO NEXT GEL * A Ready-to-Pour Acrylamide Gel for the Rapid Electrophoresis of Proteins in Standard Gels

TURBO NEXT GEL * A Ready-to-Pour Acrylamide Gel for the Rapid Electrophoresis of Proteins in Standard Gels TURBO NEXT GEL * A Ready-to-Pour Acrylamide Gel for the Rapid Electrophoresis of Proteins in Standard Gels Code Description Molecular Weight Separation Range Size M313-100ML M313-500ML M310-100ML M310-500ML

More information

Buffers & Gel Stain Chemicals

Buffers & Gel Stain Chemicals Buffers & Gel Stain Chemicals 01 Buffers & Gel Stain Buffers 3 Gel Stain SilverStar Staining Kit 6 Buffers Overview Bioneer provides over 40 types of buffer and chemical essential for life science research.

More information

BIOESSENTIALS Your guide to vital reagents and equipment for life science applications.

BIOESSENTIALS Your guide to vital reagents and equipment for life science applications. BIOESSENTIALS Your guide to vital reagents and equipment for life science applications. CELL CULTURE EXTRACTION & PURIFICATION OF BIOMOLECULES PCR & RT-PCR Separation of Nucleic Acids by Agarose Gel Electrophoresis

More information

Albumins Description Part # List Price Sale Price Discount. 45-A G $ $ % endotoxin, suitable for cell culture, ph 7, >=98%

Albumins Description Part # List Price Sale Price Discount. 45-A G $ $ % endotoxin, suitable for cell culture, ph 7, >=98% MilliporeSigma Bio Basics 2018 Stock your lab with trusted MilliporeSigma products at significantly reduced prices! Albumins Bovine Serum Albumin, lyophilized powder, essentially fatty acid free 45-A0281-10G

More information

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE)

Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) Sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) mini-gel type A. Preparation of separating gel 1. Mix distilled water, SDS, acrylamide-bis stock and 1.5 M Tris-HCl to give

More information

Appendix D: Preparation of Solutions

Appendix D: Preparation of Solutions 1. Solutions for DNA and RNA Labeling Appendix D: Preparation of Solutions 1. Solutions for DNA and RNA Labeling The reagents required for labeling depend on the labeling method chosen. For a complete

More information

XactEdit Cas9 Nuclease with NLS User Manual

XactEdit Cas9 Nuclease with NLS User Manual XactEdit Cas9 Nuclease with NLS User Manual An RNA-guided recombinant endonuclease for efficient targeted DNA cleavage Catalog Numbers CE1000-50K, CE1000-50, CE1000-250, CE1001-250, CE1001-1000 Table of

More information

PROCEDURE FOR USE NICKEL NTA Magnetic Agarose Beads (5%)

PROCEDURE FOR USE NICKEL NTA Magnetic Agarose Beads (5%) 1 AFFINITY HIS-TAG PURIFICATION PROCEDURE FOR USE NICKEL NTA Magnetic Agarose Beads (5%) DESCRIPTION Nickel NTA Magnetic Agarose Beads are products that allow rapid and easy small-scale purification of

More information

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE

Introduction. Kit components. 50 Preps GF-PC-050. Product Catalog No. 200 Preps GF-PC Preps GF-PC Preps SAMPLE Introduction The GF-1 PCR Clean Up Kit is a system designed for rapid clean up of DNA bands ranging from 100bp to 20kb. The GF-1 PCR Clean Up Kit contains special buffers to provide the correct salt concentration

More information

Ribonucleic acid (RNA)

Ribonucleic acid (RNA) Ribonucleic acid (RNA) Ribonucleic acid (RNA) is more often found in nature as a singlestrand folded onto itself. Cellular organisms use messenger RNA (mrna) to convey genetic information (using the nitrogenous

More information

Western BLoT Blocking Buffer (Fish Gelatin)

Western BLoT Blocking Buffer (Fish Gelatin) Cat. # T7131A For Research Use Western BLoT Blocking Buffer Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Storage... 3 IV. Precautions... 3 V. Protocol... 3 VI. Related

More information

AnaTag HiLyte Fluor 647 Protein Labeling Kit

AnaTag HiLyte Fluor 647 Protein Labeling Kit AnaTag HiLyte Fluor 647 Protein Labeling Kit Catalog # 72049 Kit Size 3 Conjugation Reactions This kit is optimized to conjugate HiLyte Fluor 647 SE to proteins (e.g., IgG). It provides ample materials

More information

AFFINITY HIS-TAG PURIFICATION

AFFINITY HIS-TAG PURIFICATION DESCRIPTION Nickel Affinity Cartridges 5ml are used for purification of histidine-tagged proteins in native or denaturing conditions. This cartridge can be used with an automated chromatography system,

More information

SERVA IMAC Ni-IDA Test Kit Agarose for Affinity Purification of His-Tag Fusion Proteins

SERVA IMAC Ni-IDA Test Kit Agarose for Affinity Purification of His-Tag Fusion Proteins INSTRUCTION MANUAL SERVA IMAC Ni-IDA Test Kit Agarose for Affinity Purification of His-Tag Fusion Proteins (Cat. No.42164, 42165) SERVA Electrophoresis GmbH - Carl-Benz-Str. 7-69115 Heidelberg Phone +49-6221-138400,

More information

Certificate of Analysis & Product Manual. DNA & RNA Precipitation Solutions

Certificate of Analysis & Product Manual. DNA & RNA Precipitation Solutions Certificate of Analysis & Product Manual Electrophoresis Reagents, Polymerase Chain Reaction Custom Primers and Probes Hybridization and Detection Reagents DNA & RNA Precipitation Solutions Catalog No.:

More information

Protocol for in vitro transcription

Protocol for in vitro transcription Protocol for in vitro transcription Assemble the reaction at room temperature in the following order: Component 10xTranscription Buffer rntp T7 RNA Polymerase Mix grna PCR DEPC H 2 O volume 2μl 2μl 2μl

More information

Nickel Chelating Resin Spin Columns

Nickel Chelating Resin Spin Columns 326PR-02 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Nickel Chelating Resin Spin Columns A Ni-IDA IMAC resin for 6X-His Tagged Protein

More information

Index 1. Product Description 2. Purification Procedure 3. Troubleshooting 4. Ordering Information

Index 1. Product Description 2. Purification Procedure 3. Troubleshooting 4. Ordering Information High Affinity Ni-Charged Resin Cat. No. L00223 Technical Manual No. TM0217 Version 07132010 Index 1. Product Description 2. Purification Procedure 3. Troubleshooting 4. Ordering Information 1. Product

More information

Code Description Molecular Weight Separation Range

Code Description Molecular Weight Separation Range SPRINT NEXT GEL * A Ready-to-Pour Acrylamide Gel for the Rapid Electrophoresis of Proteins Code Description Molecular Weight Separation Range Size M312-100ML M312-500ML M311-100ML M311-500ML SPRINT NEXT

More information

MOK. Media Optimization Kit

MOK. Media Optimization Kit MOK Media Optimization Kit The Media Optimization Kit determines the best medium formulation for maximizing accumulation of recombinant proteins expressed in E. coli, utilizing a series of Athena s superior

More information

A rapid, sensitive protein assay for the accurate analysis of protein concentrations

A rapid, sensitive protein assay for the accurate analysis of protein concentrations WHITE PAPER A rapid, sensitive protein assay for the accurate analysis of protein concentrations Protein quantitation is an integral part of many laboratory workflows and often a necessary step before

More information

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column.

Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using a spin-column. INSTRUCTION MANUAL ZymoPURE Plasmid Miniprep Kit Catalog Nos. D4209, D4210, D4211 & D4212 (Patent Pending) Highlights Fast and reliable purification of up to 100 µg of transfection-grade plasmid DNA using

More information

Solutions Prepare Stock Solutions:

Solutions Prepare Stock Solutions: SDS-PAGE PROTOCOL Page 1 of 5 (sodium dodecyl sulfate polyacrylamide gel electrophoresis) Is a technique widely used to separate proteins according to their electrophoretic mobility (a function of length

More information

SDS PAGE PROTOCOL SOLUTIONS

SDS PAGE PROTOCOL SOLUTIONS SDS PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) Is a technique widely used to separate proteins according to their electrophoretic mobility (a function of length of polypeptide chain

More information

Appendix IV Version

Appendix IV Version APPENDIX IV. Gel Electrophoresis. Migration of biological molecules in the presence of an electric field through a gel matrix is the heart of many biochemistry experiments. The variety of electrophoresis

More information

INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS

INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS INDEX KIT COMPONENTS 1 STORAGE AND STABILITY 1 INTRODUCTION 1 BENEFITS ERRORE. IL SEGNALIBRO NON È DEFINITO. IMPORTANT NOTES 1 METHOD FOR DNA EXTRACTION FROM AGAROSE GELS 2 METHOD FOR DNA EXTRACTION FROM

More information

Strep-Spin Protein Miniprep Kit Catalog No. P2004 & P2005

Strep-Spin Protein Miniprep Kit Catalog No. P2004 & P2005 INSTRUCTION MANUAL Strep-Spin Protein Miniprep Kit Catalog No. P2004 & P2005 Highlights Fast & Simple: Purify Strep-tagged proteins from cell-free extracts using a spin-column in 7 minutes High-Quality:

More information

His-Spin Protein Miniprep

His-Spin Protein Miniprep INSTRUCTIONS His-Spin Protein Miniprep Catalog No. P2001 (10 purifications) and P2002 (50 purifications). Highlights Fast 5 minute protocol to purify His-tagged proteins from cell-free extracts Screen

More information

SERVA Ni-NTA Magnetic Beads

SERVA Ni-NTA Magnetic Beads INSTRUCTION MANUAL SERVA Ni-NTA Magnetic Beads Magnetic beads for Affinity Purification of His-Tag Fusion Proteins (Cat. No. 42179) SERVA Electrophoresis GmbH - Carl-Benz-Str. 7-69115 Heidelberg Phone

More information

Code Description Molecular Weight Separation Range. NEXT GEL 5% Solution, 1X Includes : NEXT GEL Running Buffer, 20X

Code Description Molecular Weight Separation Range. NEXT GEL 5% Solution, 1X Includes : NEXT GEL Running Buffer, 20X * A Ready-to-Pour Acrylamide Gel for the Electrophoresis of Proteins Code Description Molecular Weight Separation Range Size M254-100ML M254-500ML M255-100ML M255-500ML M256-100ML M256-500ML M257-100ML

More information

A Picofluor method for RNA quantitation using RiboGreen

A Picofluor method for RNA quantitation using RiboGreen A Picofluor method for RNA quantitation using 1. INTRODUCTION RNA Quantitation Reagent is an ultrasensitive fluorescent nucleic acid stain. It is a simple and rapid procedure for measuring RNA concentration

More information

AnaTag HiLyte Fluor 555 Protein Labeling Kit

AnaTag HiLyte Fluor 555 Protein Labeling Kit AnaTag HiLyte Fluor 555 Protein Labeling Kit Revision number: 1.3 Last updated: April 2018 Catalog # AS-72045 Kit Size 3 Conjugation Reactions This kit is optimized to conjugate HiLyte Fluor 555 SE to

More information

AAV8 RSM Identity and Purity Assay

AAV8 RSM Identity and Purity Assay Page 1 of 11 1 PURPOSE The purpose of this assay is to determine viral identity by detecting the presence of AAV capsid proteins VP1, VP2, and VP3, their relative molecular weights and stoichiometry. This

More information

Genomic DNA Clean & Concentrator -25 Catalog Nos. D4064 & D4065

Genomic DNA Clean & Concentrator -25 Catalog Nos. D4064 & D4065 INSTRUCTION MANUAL Genomic DNA Clean & Concentrator -25 Catalog Nos. D4064 & D4065 Highlights Quick (5 minute) spin column recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC),

More information

Ni-NTA Agarose. User Manual. 320 Harbor Way South San Francisco, CA Phone: 1 (888) MCLAB-88 Fax: 1 (650)

Ni-NTA Agarose. User Manual. 320 Harbor Way South San Francisco, CA Phone: 1 (888) MCLAB-88 Fax: 1 (650) Ni-NTA Agarose User Manual 320 Harbor Way South San Francisco, CA 94080 Phone: 1 (888) MCLAB-88 Fax: 1 (650) 871-8796 www. Contents Introduction -----------------------------------------------------------------------

More information

Marketplace. Essentials Tour The bright side of PCR. molecular biology. thermofisher.com/marketplace

Marketplace. Essentials Tour The bright side of PCR. molecular biology. thermofisher.com/marketplace molecular biology Marketplace The bright side of PCR Special offers on Thermo Scientific products: Agarose powder DNA ladders Electrophoresis dyes Enhanced Taq DNA polymerases High-fidelity DNA polymerases

More information

Refold SK Protein Refolding Kit

Refold SK Protein Refolding Kit Refold SK Protein Refolding Kit 2.5mg SK v5. 2 Table of Contents Introduction...4 Kit Content...5 Instruction for Use...6 Troubleshooting Guide...9 Application Notes...16 Introduction Overexpression of

More information

E.Z.N.A. MicroElute Genomic DNA Kit. D preps D preps D preps

E.Z.N.A. MicroElute Genomic DNA Kit. D preps D preps D preps E.Z.N.A. MicroElute Genomic DNA Kit D3096-00 5 preps D3096-01 50 preps D3096-02 200 preps December 2013 E.Z.N.A. MicroElute Genomic DNA Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3

More information

AnaTag 5-FAM Protein Labeling Kit

AnaTag 5-FAM Protein Labeling Kit AnaTag 5-FAM Protein Labeling Kit Catalog # 72053 Kit Size 3 Conjugation Reactions This kit is optimized to conjugate 5-FAM SE (5-carboxyfluorescein) to proteins (e.g., IgG). It provides ample materials

More information

AnaTag HiLyte Fluor 750 Microscale Protein Labeling Kit

AnaTag HiLyte Fluor 750 Microscale Protein Labeling Kit AnaTag HiLyte Fluor 750 Microscale Protein Labeling Kit Catalog # 72044 Kit Size 3 Conjugation Reactions This kit is optimized to conjugate HiLyte Fluor 750 SE to proteins (e.g., IgG). It provides ample

More information

Reach greater highs. (and lows ) with new protein ladder choices. Fermentas now sold as. Thermo Scientific

Reach greater highs. (and lows ) with new protein ladder choices. Fermentas now sold as. Thermo Scientific Introducing Thermo Scientific Pierce Prestained and Unstained s for easy protein molecular weight determination. Fermentas now sold as Thermo Scientific Reach greater highs (and lows ) with new protein

More information

Strep-Spin Protein Miniprep Kit Catalog No. P2004, P2005

Strep-Spin Protein Miniprep Kit Catalog No. P2004, P2005 INSTRUCTION MANUAL Strep-Spin Protein Miniprep Kit Catalog No. P2004, P2005 Highlights Fast protocol to purify Strep-tagged proteins from cell-free extracts Screen your recombinant colonies directly for

More information

Renaturation Basic Kit for Proteins

Renaturation Basic Kit for Proteins Renaturation Basic Kit for Proteins Product Number 96827 Store at 2-8 C Application The Renaturation Basic Kit for Proteins is a rapid empirical screening method used to determine the best conditions for

More information

AnaTag HiLyte Fluor 488 Microscale Protein Labeling Kit

AnaTag HiLyte Fluor 488 Microscale Protein Labeling Kit AnaTag HiLyte Fluor 488 Microscale Protein Labeling Kit Revision number: 1.3 Last updated: April 2018 Catalog # AS-72048 Kit Size 3 Conjugation Reactions This kit is optimized to conjugate HiLyte Fluor

More information

AFFINITY HIS-TAG PURIFICATION

AFFINITY HIS-TAG PURIFICATION DESCRIPTION Nickel NTA Agarose Cartridges 5ml are used for purification of histidine-tagged proteins in native or denaturing conditions. This cartridge can be used with an automated chromatography system,

More information

Code Description Molecular Weight Separation Range. NEXT GEL 5% Solution, 1X Includes : NEXT GEL Running Buffer, 20X

Code Description Molecular Weight Separation Range. NEXT GEL 5% Solution, 1X Includes : NEXT GEL Running Buffer, 20X * A Ready-to-Pour Acrylamide Gel for the Electrophoresis of Proteins Code Description Molecular Weight Separation Range Size M254-100ML M254-500ML M255-100ML M255-500ML M256-100ML M256-500ML M257-100ML

More information

AFFINITY HIS-TAG PURIFICATION

AFFINITY HIS-TAG PURIFICATION DESCRIPTION Resins are products that allow batch or column purifications. This product is supplied as a suspension in 50% aqueous suspension containing 30 vol % ethanol. INSTRUCTIONS The resins are adapted

More information

Cobalt Chelating Resin

Cobalt Chelating Resin 078PR-05 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Cobalt Chelating Resin A Co-IDA IMAC resin for 6X-His Tagged Protein Purification

More information

AnaTag HiLyte Fluor 647 Microscale Protein Labeling Kit

AnaTag HiLyte Fluor 647 Microscale Protein Labeling Kit AnaTag HiLyte Fluor 647 Microscale Protein Labeling Kit Revision number: 1.3 Last updated: May 2018 Catalog # AS-72050 Kit Size 3 Conjugation Reactions This kit is optimized to conjugate HiLyte Fluor 647

More information

MagExtactor -His-tag-

MagExtactor -His-tag- Instruction manual MagExtractor-His-tag-0905 F0987K MagExtactor -His-tag- Contents NPK-701 100 preparations Store at Store at 4 C [1] Introduction [2] Components [3] Materials required [4] Protocol3 1.

More information

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology Note: for laboratory research use only RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Cat. #: RP1202 (50preps) Signalway Biotechnology I. Kit Content, Storage Condition and Stability Content

More information

On-Spin Column DNase I Kit (RNase-Free)

On-Spin Column DNase I Kit (RNase-Free) On-Spin Column DNase I (RNase-Free) Catalog No. Quantity 15100-50 Instruction Manual F Please recycle Version: 02022009 1 Introduction Use this product to remove contaminating genomic DNA from silica membrane

More information

High Pure RNA Isolation Kit for isolation of total RNA from 50 samples Cat. No

High Pure RNA Isolation Kit for isolation of total RNA from 50 samples Cat. No for isolation of total RNA from 50 samples Cat. No. 1 88 665 Principle A single reagent lyses the sample lysis and inactivates RNase. In the presence of a chaotropic salt (guanidine HCl), the released

More information

Molecular Biology. Manufactured by. Online Ordering Available

Molecular Biology. Manufactured by. Online Ordering Available Molecular Biology Manufactured by Online Ordering Available Molecular Biology Table of Contents 1 Bacterial Culture Media Powder 2 Bacterial Growth Culture Media 2 Bacterial Culture Media Broth Bottles

More information

DNAse I qualification and sample treatment

DNAse I qualification and sample treatment DNA Application Note DNAse I qualification and sample treatment INTRODUCTION The Threshold Total DNA Assay quantitates contaminant DNA in biopharmaceutical drugs. DNase I can be used in conjunction with

More information

SpeAmp n cdna Pre-amplification Kits User Guide

SpeAmp n cdna Pre-amplification Kits User Guide SpeAmp n cdna Pre-amplification Kits User Guide Cat # SpeAmp n -10 Cat # SpeAmp n -25 Cat # SpeAmp n -50 To use with our range of pathway-specific primer pools : Cat # Prim nx -10 XXXX Cat # Prim nx -25

More information

For Research Use Only. Not for use in diagnostic procedures.

For Research Use Only. Not for use in diagnostic procedures. Printed December 13, 2011 Version 1.0 For Research Use Only. Not for use in diagnostic procedures. DDDDK-tagged Protein PURIFICATION GEL with Elution Peptide (MoAb. clone FLA-1) CODE No. 3326 / 3327 PURIFICATION

More information

Product Catalogue. ID Labs Molecular Biology Products 2013

Product Catalogue. ID Labs Molecular Biology Products 2013 Product Catalogue ID Labs Molecular Biology Products 2013 Our Mission: To provide Life Science, Medical Drug Discovery Specialists with high quality, innovative and cost-effective immune and gene/chromosome-based

More information

HiPer Immunoprecipitation Teaching Kit

HiPer Immunoprecipitation Teaching Kit HiPer Immunoprecipitation Teaching Kit Product Code: HTI016 Number of experiments that can be performed: 5 Duration of Experiment Storage Instructions The kit is stable for 6 months from the date of receipt

More information

BioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only.

BioTeke Corporation. Endotoxin-free Plasmid DNA Mini-preparation Kit. (Spin-column) Note: for laboratory research use only. Note: for laboratory research use only. Endotoxin-free Plasmid DNA Mini-preparation Kit (Spin-column) Cat. # DP2601 (20 preps) DP2602 (50 preps) BioTeke Corporation 1 I. Kit Content Storage and Stability

More information

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format)

Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit

More information

Western Blot Protocol

Western Blot Protocol Western Blot Protocol Western blotting (WB) is the most widely performed immunoassay and is the best initial validation technique used to identify proteins of interest within a tissue homogenate or cell

More information

Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions

Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Plasmid DNA Isolation Column Kit Instruction Manual Catalog No. SA-40012: 50 reactions SA-40011: 100 reactions Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800) 989-6296

More information

Terminator 5 -Phosphate- Dependent Exonuclease

Terminator 5 -Phosphate- Dependent Exonuclease Terminator 5 -Phosphate- Dependent Exonuclease Cat. No. TER51020 www.lucigen.com MA246E-Terminator 5 -Phosphate-Dependent Exonuclease 11/2017 1 1. Introduction Terminator 5 -Phosphate-Dependent Exonuclease

More information

What is DNA. DNA DNA is the genetic material. Is Is a double helix. Made Made up of subunits called nucleotides Nucleotide

What is DNA. DNA DNA is the genetic material. Is Is a double helix. Made Made up of subunits called nucleotides Nucleotide What is DNA DNA DNA is the genetic material. Is Is a double helix. Made Made up of subunits called nucleotides Nucleotide made of: 1. hosphate group 2. 5-carbon sugar 3. Nitrogenous base 2 O 4' T 1' to

More information

HiPer Gel Extraction Teaching Kit (Column Based)

HiPer Gel Extraction Teaching Kit (Column Based) HiPer Gel Extraction Teaching Kit (Column Based) Product Code: HTBM010 Number of experiments that can be performed: 10 Duration of Experiment Agarose Gel Electrophoresis: 1 hour Protocol: 1 hour Agarose

More information

E.Z.N.A. Cycle Pure Kit

E.Z.N.A. Cycle Pure Kit E.Z.N.A. Cycle Pure Kit D6492-00 5 preps V-spin D6492-01 50 preps V-spin D6492-02 200 preps V-spin D6493-00 5 preps Q-spin D6493-01 50 preps Q-spin D6493-02 200 preps Q-spin March 2017 E.Z.N.A. Cycle Pure

More information

E.Z.N.A. Cycle Pure Kit

E.Z.N.A. Cycle Pure Kit E.Z.N.A. Cycle Pure Kit D6492-00 5 preps V-spin D6492-01 50 preps V-spin D6492-02 200 preps V-spin D6493-00 5 preps Q-spin D6493-01 50 preps Q-spin D6493-02 200 preps Q-spin March 2017 E.Z.N.A. Cycle

More information

ProductProfile. Bioreagents that deliver the purity you need with the consistency you ve come to expect. The most informative reading in bioscience.

ProductProfile. Bioreagents that deliver the purity you need with the consistency you ve come to expect. The most informative reading in bioscience. ProductProfile Bioreagents that deliver the purity you need with the consistency you ve come to expect. The most informative reading in bioscience. Molecular biology procedures often require the use of

More information

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps E.Z.N.A. Blood DNA Maxi Kit D2492-00 2 preps D2492-03 50 preps May 2013 E.Z.N.A. Blood DNA Maxi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps

E.Z.N.A. Blood DNA Maxi Kit. D preps D preps E.Z.N.A. Blood DNA Maxi Kit D2492-00 2 preps D2492-03 50 preps April 2014 E.Z.N.A. Blood DNA Maxi Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing Reagents...4

More information

GENERAL BIOLOGY LABORATORY II

GENERAL BIOLOGY LABORATORY II Weeks 9-10: Bioassays of major biomolecules: Nucleic acids GENERAL BIOLOGY LABORATORY II Canbolat Gürses, Hongling Yuan, Samet Kocabay, Hikmet Geckil Department of Molecular Biology and Genetics Inonu

More information

ZR-96 Genomic DNA Clean & Concentrator -5 Catalog Nos. D4066 & D4067

ZR-96 Genomic DNA Clean & Concentrator -5 Catalog Nos. D4066 & D4067 INSTRUCTION MANUAL ZR-96 Genomic DNA Clean & Concentrator -5 Catalog Nos. D4066 & D4067 Highlights 96-well plate recovery of large-sized DNA (e.g., genomic, mitochondrial, plasmid (BAC/PAC), viral, phage,

More information

NOTE ACRYLAMIDE IS NEUROTOXIN YOU MUST WEAR GLOVES.

NOTE ACRYLAMIDE IS NEUROTOXIN YOU MUST WEAR GLOVES. GST Purfication and Pulldown Part I Instructor: David Deitcher TA: Kristy Lawton In order to study the function of a protein it is often useful to have that protein purified away from others in the cell.

More information

WesternMAX Alkaline Phosphatase Chemiluminescent Detection Kits

WesternMAX Alkaline Phosphatase Chemiluminescent Detection Kits WesternMAX Alkaline Phosphatase Chemiluminescent Detection Kits Code N221-KIT N220-KIT Description WesternMAX Chemiluminescent AP Kit, Anti-Mouse Includes: Alkaline Phosphatase (AP) Conjugated Anti-Mouse

More information

DENAzist Asia's High Quality Kits and Molecular Biology Products Price list_version Cat. No. Name Application Price (Rial)

DENAzist Asia's High Quality Kits and Molecular Biology Products Price list_version Cat. No. Name Application Price (Rial) RNA Isolation kits S1010 Total RNA isolation kit (25) A unique combination of solutions for extraction of RNA from cells, tissues, 1,190,000 S10101 Total RNA isolation kit (50) plants, bacteria, yeasts.

More information

PureSpin DNA Clean Up Kit

PureSpin DNA Clean Up Kit PureSpin DNA Clean Up Kit Micro Columns INSTRUCTION MANUAL KIT COMPONENTS For Research Use Only PureSpin DNA Clean Up Kit, Micro Columns w/out Caps (Kit Size) OD2080 (50 Preps.) OD2080-2 (200 Preps.) Storage

More information

ENDEXT Technology. Instruction manual for protein synthesis. with wheat germ cell-free system

ENDEXT Technology. Instruction manual for protein synthesis. with wheat germ cell-free system ENDEXT Technology Instruction manual for protein synthesis with wheat germ cell-free system 1 Protocol Overview Plasmid DNA construction (see Section 3.1) Preparation of plasmid DNA for transcription (see

More information

ADDENDUM. Ready Gel System RESOURCE GUIDE. Applications:

ADDENDUM. Ready Gel System RESOURCE GUIDE. Applications: Ready Gel System RESOURCE GUIDE ADDENDUM This addendum includes new electrophoresis products introduced since the Ready Gel Resource Guide was published and updated information for a few products. Keep

More information

ChIP DNA Clean & Concentrator Catalog Nos. D5201 & D5205

ChIP DNA Clean & Concentrator Catalog Nos. D5201 & D5205 Page 0 INSTRUCTION MANUAL ChIP DNA Clean & Concentrator Catalog Nos. D5201 & D5205 Highlights Quick (2 minute) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP) assays, cell lysates,

More information

University of Guelph. Supply Center Product List

University of Guelph. Supply Center Product List University of Guelph Supply Center Product List Supply Center Location 488 GORDON ST SCI COMPLX BLDG 140 IVGN SPLY To find out more about your Supply Center, request product additions to the Supply Center,

More information

ProductInformation TECHNICAL BULLETIN MULTIPLE TISSUE NORTHERN BLOT, MOUSE. Product No. BLOT-2 Technical Bulletin No. MB-865 February 2000

ProductInformation TECHNICAL BULLETIN MULTIPLE TISSUE NORTHERN BLOT, MOUSE. Product No. BLOT-2 Technical Bulletin No. MB-865 February 2000 MULTIPLE TISSUE NORTHERN BLOT, MOUSE Product No. BLOT-2 Technical Bulletin No. MB-865 February 2000 ProductInformation TECHNICAL BULLETIN Product Description Sigma s Mouse Multiple Tissue Northern Blot

More information

PART 1 LAB ESSENTIALS BACK TO LAB PROMOTION. Buy 2 Get 3rd Free. Get started in the lab with these great offers on lab essentials!

PART 1 LAB ESSENTIALS BACK TO LAB PROMOTION. Buy 2 Get 3rd Free. Get started in the lab with these great offers on lab essentials! BACK TO LAB PROMOTION LAB ESSENTIALS PART 1 Get started in the lab with these great offers on lab essentials! DNA Markers and Protein Laddders DNA Polymerases and Agarose Buy 2 Get 3rd Free Plasmid Purification

More information

Instant-Bands Protein Sample Loading Buffer for SDS-PAGE. User s Manual. View Protein Bands in an SDS Gel. Instantly. EZBiolab.

Instant-Bands Protein Sample Loading Buffer for SDS-PAGE. User s Manual. View Protein Bands in an SDS Gel. Instantly. EZBiolab. Instant-Bands Protein Sample Loading Buffer for SDS-PAGE User s Manual View Protein Bands in an SDS Gel Instantly www.ezbiolab.com 2 Instant-Bands User s Manual Table of Contents Introduction 3 Storage

More information

E.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps

E.Z.N.A. HP Viral RNA/DNA Kit. R preps R preps E.Z.N.A. HP Viral RNA/DNA Kit R6873-00 5 preps R6873-01 50 preps August 2013 E.Z.N.A. HP Viral RNA/DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

AminTRAP HIS Prepacked Column

AminTRAP HIS Prepacked Column INDEX Ordering Information... 3 Intended Use... 3 Product Description... 3 Purification Procedure... 4 Sample Preparation... 5 Sample Purification... 6 Analysis... 6 Regeneration Procedure... 6 Use and

More information

Importance of Molecular Genetics

Importance of Molecular Genetics Molecular Genetic Importance of Molecular Genetics Genetics is playing an important role in the practice of clinical medicine. - Medical genetics involves any application of genetics to medical practice,

More information

RASAQ NURUDEEN OLAJIDE

RASAQ NURUDEEN OLAJIDE RASAQ NURUDEEN OLAJIDE LECTURE CONTENT INTRODUCTION SOUTHERN BLOTTING WESTERN BLOTTING DNA PROFILING (DNA FINGERPRINTING) PARENTAL TESTING PROCEDURES INTRODUCTION Then the Lord said to Cain, Where is your

More information

Bacterial PE LB. Bacterial Protein Extraction Lysis Buffer. (Cat. # , , , , , )

Bacterial PE LB. Bacterial Protein Extraction Lysis Buffer. (Cat. # , , , , , ) G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name Bacterial PE LB Bacterial Protein Extraction Lysis Buffer (Cat. # 786-176, 786-177, 786-185,

More information