Coats Agrialoe LLC. GroAloe

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1 Coats Agrialoe LLC. GroAloe

2 The Aloe Vera Soil Additive What is it? GroAloe is a soil amendment product introduced by Coats AgriAloe. Derived from organically grown aloe vera gel from whole leaves, GroAloe is developed as an alternative to more traditional, chemical-rich amendments. What Does It Do? Like many soil amendments, GroAloe helps improve soil conditions and makes things easier to grow. Where GroAloe differs is that it doesn t have harmful chemicals, which helps promote chelation in the soil, increasing nutrients in soil.

Natural and Safe Soil Amendment GroAloe is made using the Coats Patented Process, ensuring safe and effective nutrients for your home garden or crop fields.

4 Natural and Safe Soil Amendment GroAloe is made using the Coats Patented Process, ensuring safe and effective nutrients for your home garden or crop fields. Coats AgriAloe ensures the aloe plants are processed within hours of being harvested to preserve the efficacy of the beneficial aloe properties. The aloe is then transported using temperature controlled storage to maintain the purity and efficacy of the patented whole-leaf process. All Aloe is measured and tested for the purest, effective ingredients possible. That's why we guarantee that our Aloe Vera gel from whole leaf is 99.4% per bottle. GroAloe is suitable for use in areas where synthetic chemicals cannot be used. It contains Lignin, a natural biopolymer that enhances nutrient uptake and promotes efficient water absorption by the plant. In addition, GroAloe contains carbohydrates and amino acids, which promote the chelation of minerals present in the soil, allowing the plants to readily absorb nutrients. GroAloe is produced from Organically Grown Whole Leaf Aloe Vera that has been industrially masticated into a liquid or powdered medium, under the patented Coats process.

5 Why GroAloe Is Better For You and Your Environment Safe Nutrients for a Healthier Food GroAloe does not contain any hazardous ingredients. 99.4% natural and eco-friendlyitable for use in areas where synthetic chemicals cannot be used.enhances nutrient uptakecontains Lignin, a natural biopolymer, which allows plants to utilize the food at its root system.a faster drench promotes efficient water usage.promotes chelation in the soil, which increases nutrients in soilenhances the benefits of fertilizers The guaranteed analysis of Coats Agrialoe GroAloe: 99.4% Aloe Vera gel from Whole Leaf (using Coats patented process) Coats AgriAloe buys the highest quality of aloe Aloe Vera Barbadensis Miller from the island of Barbados to ensure the highest quality end product. Not all aloe vera is created equal. With over 240 different species of aloe, only four are identified as suitable for human consumption. Aloe Vera Barbadensis Miller is the top of the heap. Why is Aloe Vera Barbadensis Miller the best for your soil? It contains over 20 amino acids, important for soil chelation, which contributes to nutrient uptake. It contains carbohydrates, such as mannitol, also important for soil chelation. It is naturally enriched with a host of vitamins including A, B1, B2, B6, B12, C and E, and micro-nutrient minerals including calcium, manganese, magnesium, zinc, and copper. It has the highest efficacy of any known strain of aloe vera.

6 Our Mission To improve the global food chain and agricultural marketplace and feed an expanding population with a food supply that is safe, healthy and nutritious.

Pioneers of the Aloe Industry One of the pioneers of Aloe Vera research, development and global technology, Bill Coats is considered a preeminent authority by his peers on the subject of Aloe Vera.

7 Pioneers of the Aloe Industry One of the pioneers of Aloe Vera research, development and global technology, Bill Coats is considered a preeminent authority by his peers on the subject of Aloe Vera. His many accomplishments in the field of Aloe Vera research, development, promotion, production and world education have earned him a well - deserved reputation as Aloe's most effective advocate. For this reason, Mr. Coats has been able to attract some of the most highly regarded professionals from the fields of science, agriculture and global marketing to become members of the Coats AgriAloe management and research team. These individuals share both the passion and discipline to advance the same criteria of excellence established over the years by Bill Coats. This gathering of the "best and brightest" minds in their field to the Coats AgriAloe banner provides the ideal combination of dedication, discipline, and professional credibility to gain both acceptance and advancement for all Coats AgriAloe initiatives.

8 Patented Formula In the last 60 years numerous attempts were made to isolate, extract and replicate the effective ingredients in Aloe Vera. Each attempt was met with only partial success or complete failure. The chemistry of Aloe Vera still defies complete analysis and replication. In 1964, Bill Coats, then a practicing pharmacist in Dallas, Texas, became a man obsessed with finding a way to isolate and stabilize the active ingredients inside the Aloe Vera leaf. Normally, these active ingredients inside the Aloe Gel, once exposed to light and air, oxidize and become useless within 4 hours of harvest. Mr. Coats dedicated himself to learning the micro-biology of the Aloe Vera Plant to extract and stabilize the plant gel while preserving it's natural curative potency. In 1968 he succeeded in his mission to create and patent one of the very first successful stabilization patents now used in Aloe Vera drinks, skincare, medicine and more today. Under U.S. Patent #5,356,811 granted by the U.S. Patent Office to Bill C. Coats, R.Ph., C.C.N., Coats Whole Leaf Aloe Vera is processed under rigorous quality control standards.

9 Results From Major Institutions and Universities Around The Globe

10 Indonesian Center for Rice Research (IRIR) Preliminary Report GroAloe Test Over the past three months the I.R.I.R. was conducting tests on rice using GroAloe in various strength configurations in conjunction with NPK fertilizer (N)Nitrogen (P)Phosphorus (K)Potassium at different dosage percentages. The information below is a synopsis of their results. The average yield of rice per hectare per season is 4.7 metric tons (Central Research Institute for Food Crops) using chemical fertilizer and pesticides. Without using either NPK or GroAloe the average yield was only 4.03 metric tons per hectare. Using 100% of the NPK dosage and 4% dilution of GroAloe the yield increased to 6.97 metric tons per hectare, delivering an increase in production of 2.94 metric tons ( lbs) per hectare over there national average listed above. Based on the current Indonesian market (Index Mundi, March 2013) for white rice of $ per metric ton, using GroAloe increased the revenue of each hectare by $1, USD Or 48%. In comparison the US average rice production converted into hectares from acres is 6.91 metric tons (NASS Rice Report ) GroAloe was tested at two strengths of dilution 6% and 4%. While both dilutions showed increases in production, the lower 4% performed the best in every category with the NPK added. The report concludes the following GroAloe consistently is effective for rice plants either with or without NPK fertilizer at different levels. GroAloe treated plants were not attacked by pests or diseases, there were no chemical insecticides or fungicides were used on any plants during this test. GroAloe is effective for growth, yield components and grain yields.

11 IRRI Testing Facility

20 World top 10 Rice Producing Countries 2013 Most Rice Producing Countries in the World Rank Country Name Rice Production (1000 Metric Ton) 1 China India Indonesia Bangladesh Vietnam Thailand Philippines Myanmar Brazil Japan 7500

21 Letter of Appointment of Representative to the Indonesian Agricultural Ministry 8 August 2014 To Whom It May Concern Dear Sir/Madam Letter of Appointment I am Billy C. Coats, the Owner of Coats AgriAloe LLC. USA (hereinafter the Company) and having an address at 9660 Dilworth Road Dallas, Texas USA. On behalf of the Company, we are pleased to appoint: Company: PT Agro Aloe Indonesia Name: Jenny Arifin Position: Director Address: Perum Resinda Blok D3 No. 11RT./RW.001/009, Kel, Purwadana, Kee. Timur, Kab. Karawang -Jawa Barat.INDONESIA TelukJambe As our authorized distributor and reseller for fertilizer Coats AgriAloe product,groaloe in Indonesia for the period of five (5) years from the date of this letter. Renewal is by mutual agreement and consent. Both parties reserve the right to terminate this appointment by giving one (1) month notice. We highly appreciate your interest in our product and we look forward to a prosperous business your company. Sincerely, Billy C. Coats C.C.N., R.Ph. President/CEO Coats Agri Aloe LLC. relationship with

The Center is part of the Texas A&M University System and has faculty from the Texas Agricultural Experiment

22 GroAloe Research here the USA The Texas A&M AgriLIFE Research Center at Beaumont/Eagle Lake provides educational support for rice production, as well as the alternative crops associated with rice. The Center is part of the Texas A&M University System and has faculty from the Texas Agricultural Experiment Station, Texas Cooperative Extension, Texas A&M University, and the United States Department of Agriculture Agricultural Research Service.

23 2015 Proposed Research for Coats AgriAloe Applied to Rice Drs. Shane Zhou and Lee Tarpley Texas A&M Agrilife Research and Extension Center, Beaumont, TX Statement of the Problem and Research Background: Sheath blight, caused by the fungus Rhizoctonia solani, is the most important disease in rice in the US and is one of the most important diseases in other rice-producing countries. The disease causes significant losses in grain yield and quality. None of the commercially available rice cultivars have acceptable levels of resistance against sheath blight. Rice farmers heavily depend on fungicides for control of the disease. Excessive use of fungicides have caused fungicide resistance and increased production costs. Farmers are in need of alternative and sustainable strategies for control of sheath blight and other diseases. Results of our recent lab research demonstrate the ability of Coats AgriAloe to suppress the growth of the fungus R. solani. A separate lab research also demonstrates the ability of Coats AgriAloe to inhibit the spore germination of the fungus Cercospora janseana, the casual agent of narrow brown leaf spot. These in vitro results indicate the potential effectiveness of Coats AgriAloe for control of sheath blight and narrow brown leaf spot at the stages of primary and spread infection in the field. Results of 2013 and 2014 field trials further validated the efficacy of the product for management of narrow brown leaf spot. In addition, results of our field trials in 2013 and 2014 also demonstrated positive impacts of Coat AgiAloe on rice plant physiology.

24 Conclusion Statement from Dr. Shane Zhou Texas A&M AgriLife Research Center Beaumont Texas 2014 Testing of GroAloe Conclusion and Recommendations: Foliar sprays of AgriAloe provide small but significant control of narrow brown leaf spot. The inhibition of spore germination of the pathogen by AgriAloe is one of the mechanisms associated with AgriAloe-induced disease suppression. More studies are needed to verify the results of this study in the future. It would be very interesting to conduct field and lab experiments to determine if AgriAloe can also control sheath blight caused by the fungus Rhizoctonia solani, the most important disease in the US and the world. Proved efficacy of AgriAloe for control of sheath blight will significantly increase the marketability of this product domestically and internationally.

25 Evaluation of Coats AgriAloe for Control of Rice Sheath Blight Pathogen Dr. Shane Zhou Texas A&M AgriLife Research Center, Beaumont, TX Sheath blight, caused by the fungus Rhizoctonia solani AG1-IA, is the most important disease in rice in the US and is one of the most important diseases in other rice-producing countries. The disease causes significant losses in grain yield and quality each year. Sheath blight can cause as much as 50% yield loss. None of the commercially available rice cultivars have acceptable levels of resistance against sheath blight. Rice farmers heavily depend on fungicides for control of the disease. Excessive use of fungicides have caused fungicide resistance and increased production costs. Farmers are in need of alternative and sustainable strategies for control of sheath blight. Objective of this research was to evaluate the efficacy of Coats AgriAloe for control of R. solani in an effort to develop a novel biocontrol approach for management of sheath blight and improvement of grain yield in rice. Experiments were conducted in the lab to evaluate the effects of Coats AgriAloe on the myceliumgrowth of R. solani and on the germination of sclerotia and the mycelium growth from germinated sclerotia. Sclerotia and mycelium in infected residue are the primary inoculum inducing disease development at the beginning of disease cycle. Runner hyphae (mycelium) are the only means for this fungus to infect and spread the disease during the cropping season. Control of mycelium growth and sclerotium germination is the key to the effective management of sheath blight Aggie Drive Beaumont, Texas Tel Ext Fax xzhou@aesrg.tamu.edu WWW

26 Effects of Coats AgriAloe on the mycelium growth of R. solani Two strains of R. solani, TX-RS1 and TX-RS12, were used in the experiment. Both pathogen strains were collected from rice plants showing symptoms of sheath blight in commercial rice fields in Texas. Each strain was grown on potato dextrose agar (PDA) plates at room temperature for 2 days before use. AgriAloe in powdery formula was diluted in sterile distilled water and added into melted PDA medium to reach the concentrations of 4, 8 and 16% (v/v). PDA without AgriAloe amendment served as the control (0%, v/v). Plugs (0.65 cm in diameter) of mycelium were cut from the edge of an actively growing fungal colony on PDA with a cork borer and one of the plugs was placed in the center of each PDA plate (10 cm in diameter). The plates were incubated at room temperature. After 24 hours of incubation, the diameter of the growth of mycelium from each of the plugs was measured. There were three replicates (plates) for each treatment. Each test was conducted two times. AgriAloe applied at the concentrations of 4, 8 and 16% significantly inhibited the growth of mycelium of both isolates of R. solani evaluated (Fig. 1 and Table 1). The degree of mycelium growth inhibition linearly increased with the increase of concentration of AgriAloe (Fig. 2).The mycelium growth inhibition efficacy reached more than 55% when the concentration increased to 16%.

27 Fig. 1. Effects of Coats AgriAloe concentration on the growth of mycelium of strains TX-RS1 and TX-RS12 of R. solani. Marked contours are for easy visulization of colonies.

28 Table 1. Effects of different concentrations of Coats AgriAloe on the growth of mycelium of two strains of R. solani Mycelium growth diameter (cm) AgroAloe concentration (%, v/v) TX-RS1 TX-RS a* 6 a b 4.4 b c 3.7 c d 2.6 d * Means were the average of three replicated plates. Means within a column with the same letter are not significantly different (P = 0.05) according to Fisher s protected LSD test.

29 Fig. 2. Effects of Coats AgriAloe concentration on the growth of mycelium of strains TX-RS1 (A) and TX-RS12 (B) of R. solani.

30 Effects of Coats AgriAloe on the germination of sclerotia and the mycelium growth from germinated sclerotia of R. solani Two strains of R. solani, TX-RS1 and TX-RS12, were also used in the experiment. Each strain was grown on PDA plates at room temperature under the dark for four weeks to produce sclerotia. Uniform sclerotia in size and color were selected for the experiment. AgriAloe in powdery formula was diluted in sterile distilled water and added into melted PDA medium to reach the concentrations of 4, 8 and 16% (v/v). PDA without AgriAloe amendment served as the control (0%, v/v). Five sclerotia were placed evenly at distance on each PDA plate (10 cm in diameter) (Fig. 3). After 24 hours of incubation at room temperature, the number of sclerotia germinated on each plate was counted and converted to percent germination of sclerotia. The diameter of the mycelium growth from the germinated sclerotia was also measured for each colony. There were seven replicates (plates) for each treatment. The experiment was repeated twice. AgriAloe applied at any of the concentrations (4, 8 and 16%) was not effective in reducing percent germination of sclerotia of either isolate of R. solani evaluated (Table 2). However, all the concentrations of AgriAloe tested significantly reduced the mycelium growth from germinated sclerotia (Fig. 3 and Table 3). The degree of mycelium growth inhibition linearly increased with the increase of concentration of AgriAloe (Fig. 4). The mycelium growth inhibition efficacy reached more than 50% when the concentration increased to 16%.

31 Fig. 3. Effects of Coats AgriAloe concentration on the mycelium growth from germinated sclerotia of strains TX-RS1 and TX-RS12 of R. solani. Marked contours are for easy visulization of colonies.

32 Table 2. Effects of different concentrations of Coats AgriAloe on the germination of sclerotia of two strains of R. solani Germination of sclerotia (%) * AgroAloe concentration (%, v/v) TX-RS1 TX-RS a* 94 a a 94 a a 100 a a 86 a Means were the average of three replicated plates. Means within a column with the same letter are not significantly different (P = 0.05) according to Fisher s protected LSD test.

33 Table 3. Effects of different concentrations of Coats AgriAloe on the mycelium growth from germinated sclerotia of two strains of R. solani Mycelium growth diameter (cm) AgroAloe concentration (%, v/v) TX-RS1 TX-RS a* 1.6 a c 1.3 b b 1.1 b c 0.8 c

34 A B Fig. 4. Effects of Coats AgriAloe concentration on the mycelium growth from germinated sclerotia of strains TX-RS1 (A) and TX-RS12 (B) of R. solani.

35 Conclusion and Recommendations Results of these studies demonstrate the ability of AgriAloe product to inhibit the growth of mycelium of the sheath blight pathogen. AgriAloe also has the ability to reduce the mycelium growth from germinated sclerotia. All of these findings are very significant. These in vitro results indicate the potential effectiveness of AgriAloe for control of sheath blight pathogen at the stages of primary and spread infection in the field conditions. Therefore, it is strongly recommended that a field trial should be conducted in 2015 to validate the efficacy of AgriAloe product for control of sheath blight and increase of grain yield. Proved efficacy of AgriAloe for control of sheath blight will significantly increase the marketability of this product domestically and internationally.

36 Conclusion Statement from Dr. Lee Tarpley Texas A&M AgriLife Research Center Beaumont Texas 2014 Testing of GroAloe No detrimental effect, plus a significant increase in net photosynthesis. In summary, in 2014 as was the case in 2013, the AgriAloe treatments did not have detrimental effects on the rice plant physiology, suggesting the adequacy of the time-of-day of application, and further suggesting that if AgriAloe provides other benefits, such as repellant ability, then these may be achieved without harm to the physiological status of the plant. Furthermore, the data trends again suggest a positive benefit on physiological parameters related to photosynthetic ability, namely an apparent increase in leaf greenness (SPAD), which is associated with a significant gain in net photosynthesis (PN).

37 Conclusion Statement from Dr. Shane Zhou Texas A&M AgriLife Research Center Beaumont Texas 2013 Testing of GroAloe Conclusion and Recommendations: Foliar application of AgriAloe appeared to be effective in reducing narrow brown leaf spot, one of the major diseases affecting rice production in Texas. Failure of AgriAloe seed treatment for control of seedling diseases in this trial may be due to the low disease pressure. The lower soil temperatures at planting, the more severe are seedling diseases. It is strongly recommended that this field trial be evaluated at the early or normal planting date when soil temperatures are low enough to induce seedling diseases.

38 Conclusion Statement from Dr. Lee Tarpley Texas A&M AgriLife Research Center Beaumont Texas 2013 Testing of GroAloe In summary, the AgriAloe treatments did not have detrimental effects on the rice plant physiology, suggesting the adequacy of the time-of-day of application, and further suggesting that if AgriAloe provides other benefits, such as repellant ability, then these may be achieved without harm to the physiological status of the plant. Furthermore, the data trends suggest a positive benefit on physiological parameters related to photosynthetic ability, namely an apparent increase in leaf greenness which potentially carried over as possible benefits in several other photosynthetic parameters. These trends, however, were not significant, so would require additional testing before any statement of benefit could be made..

39 Coats Agrialoe GroAloe Research at the Texas A&M University Kingsville Citrus Center - Weslaco

40 E. australis (SOS) Cause and Effect Sweet orange scab (SOS) is a disease caused by the fungus Elsinöe australis. This disease is aptly named as it results in scab-like lesions that develop primarily on the fruit rind and infrequently, on leaves and twigs. Although there is little affect on internal fruit quality, fruit are severely blemished rendering them unsellable in the fresh produce market. Further, the disease can cause premature fruit drop and stunt young nursery trees and new field plantings. If citrus disease were to spread out of control, it has the potential to destroy the entire U.S. citrus industry, causing the loss of billions of dollars and millions of jobs.

41 GroAloe Initial Testing on E. australis (SOS) By Texas A&M Citrus Center and the U.S.D.A. Recovery of E. australis from grapefruit fruits treated with 10% or 12% of Agriloe for 2 or 3 minutes. z Values with the same letter are not significantly different at P=0.05 Since these experiments were not repeated, data obtained should be considered as preliminary. However, it appears that Agriloe has some antifungal properties against E. australis. Future studies with Agriloe should include other products used by citrus organic packinghouses for comparison purposes. Treatment Control Host Grapefruit Number of fruits tested 360 Fruits with E. australis (%) 44.2 az Agriloe 10% Grapefruit b Agriloe 12% Grapefuit b

42 2015 Testing This years tests will involve many different aspects from pre-bloom, maturation and pre harvest to post harvest treatment. It is our belief that GroAloe will be able to penetrate the tree and kill the fungus at tree level as well during procedure once it arrives at the packing facility. To date Coats patented process Aloe Vera has destroyed every fungal, bacterial and viral pathogen it has been tested against. Given the correct strength and time allotment in the laboratory. And we are convinced this is the best and natural cure to protect Citrus crops both here in the USA and around the world.

43 GroAloe the Safe, Natural and Affordable Soil Amendment for all Crops Vegetable Crops Grain Crops Tree Fruits Bush Fruits Nut Crops Coffee Beans Tea Bushes

50 Coats Agrialoe GroAloe Farming for the 21st Century

No matter the weather conditions, there will be problematic diseases every year. Which disease may change from year to year.

No matter the weather conditions, there will be problematic diseases every year. Which disease may change from year to year. This presentation focuses on integrated pest management of corn and soybean