CHEMOMETRICAL ANALYSIS OF ENDOMETRIAL TISSUE FLUORESCENCE SPECTRA
|
|
- Adam Logan
- 6 years ago
- Views:
Transcription
1 CHEMOMETRICAL ANALYSIS OF ENDOMETRIAL TISSUE FLUORESCENCE SPECTRA A.Vaitkuviene a *, E.Auksorius a, D. Fuchs b, V.Gavriushin a a Vilnius University, Institute of Materials Science and Applied Research, b Innsbruck University, Institute of Medical Chemistry and Biochemistry, and Ludwig Boltzmann Institute of AIDS-Research. ABSTRACT An effort has been made to detect neopterin spectrum in fluorescence of premalignant endometrial tissue and to estimate the number of fluorophores naturally existing in the tissue with fluorescence present above the noise level. Endometrial Tissue fluorescence was measured in vitro by excitation with the third harmonic of Nd YAG laser. Multivariate curve resolution was used for testing neopterin presence in endometrial tissue. Fluorescence spectra of neopterin was measured and used as a target spectrum for testing. Seven factors fluorescence of natural fluorophores of endometrial tissue were found to be present above the noise level in the overall autofluorescence. Neopterin concentration may be too low in endometrial tissue to make its fluorescence above the noise level because neopterin spectrum was not found to be among the spectra resolved by multivariate curve resolution. An intensity increase in the neopterin spectrum spectral region in hyperplastic endometrial samples might be associated with neopterin concentration increase. Keywords: Biomarker, Laser induced fluorescence spectroscopy, Multivariate curve resolution. 1. INTRODUCTION Diagnostic techniques based on optical spectroscopy have the potential to link the biochemical and morphological properties of tissues to individual patient care. If applied successfully, optical spectroscopy has the potential to represent an important step forward toward advances in diagnostic and therapeutic medical applications.currently, one of the most widely explored applications of fluorescence spectroscopy is the detection of endoscopically invisible, early neoplastic growth in epithelial tissue sites. There are no effective diagnostic techniques for these early tissue transformations. If fluorescence spectroscopy can be applied successfully as a diagnostic technique in this clinical context, it may increase the potential for curative treatment and thus reduce complications. Fluorescence spectroscopy is ideally suited for this application because of its ability to examine tissue surfaces rather than tissue volumes, and its adaptability to an endoscopic device. Biological molecules that exhibit endogenous fluorescence are amino acids, structural proteins, enzymes and coenzymes, vitamins, lipids and porphyrins. Their excitation maxima lie in the range nm, whereas their emission maxima lie in the range nm [1]. The endogenous fluorophores that are speculated to play a role in transformations that occur with carcinogenesis are the amino acids tryptophan and tyrosine, the structural proteins collagen and elastin, the coenzymes NADH and FAD and porphyrins biological processes. * Correspondence: Women Clinics, Vilnius University, Saulėtekio al. 6 III 2040 Vilnius, Lithuania Laser Florence 2001: A Window on the Laser Medicine World, Leonardo Longo, Alfons G. Hofstetter MihaiI Lucian Pascu, Wilhelm R. A. Waidelich Editors Proceedings of SPIE Vol 4903 (2002) SPIE /01/$15.00
2 The altered biochemical and morphological state that occurs as tissue progresses from a nondiseased to a diseased state is detected in the spectral characteristics of the measured fluorescence. Neopterin is a marker of stimulation of celular immune response. Tumor necrosis factor-α enhances interferon induced neopterin production. Neopterin concentration is higher in patients with a malignant tumor. Therefore, neopterin seems to be an important component of spectral analysis of premalignant and malignant tissue [2]. Information about biological molecules presence or absence in tissue and their concentration might be of particular importance. Multivariate curve analysis (MCR) provides the number of chemical species present in the sample, the spectrum of each species for identification, and the concentration for each species. Curve resolution is chemometrical tool based on the determination of qualitative information and the recovery of response profiles. A lot of modifications of curve resolution techniques and applications have been published [3,4]. The traditional curve resolution techniques can be adapted to analyse fluorescence spectra in order to resolve data into individual concentration and pure spectra. The additional benefit of spectra noise reduction also results from the MCR techniques. 2. MATERIALS AND METHODS Materials. Sixty patients were included in the study. Endometrial tissues were biopsied and classified by routine histopathology as Normal, Hyperplastic (HP) or Cancerous (CN). The 36 normal, 19 hyperplastic and 5 cancerous samples were under study. Laser induced fluorescence measurements in vitro were performed with laser as excitation source. NAD:YAG laser third harmonic (3,51eV) providing 70 ns pulses and 1Hz repetition rate was used. Data matrix - fluorescence spectra of 60 samples consisting of 220 points each were obtained. Thus, Data matrix consisting of 220 columns (wavelength) and 60 rows (samples) were analysed. A fluorescence spectrum of neopterin (Schircks-Laboratories, Jona, Switzerland) was measured. Chemometrics. Chemometrics is a part of chemistry that develops mathematical and statistical methods for analysing chemical data. Multivariate curve resolution is a chemometrical tool based on the determination of qualitative information and the recovery of response profiles. Multivariate curve analysis provides the number of chemical species present in the sample, the pure spectrum of each species for identification, and the concentration for each species. The pure spectra obtained by multivariate curve resolution can be used to identify chemical species using library spectra.in the curve resolution methods, an iterative least squares optimisation procedure is implemented. Multivariate curve resolution consisting of principal component analysis combined with non-oblique factor rotation and alternating least-squares optimisation was used for fluorophores present in endometrial tissue spectral and concentration profiles resolution. Principal component analysis creates a set consisting of a small number of specially chosen basis spectra, called principal components, which can accurately characterize all of the spectral changes in a set of spectral data. Statistically significant principal components are called factors. However, factors are not yet directly related to any real chemical spectra (they, for example, are negative). Non-oblique factor rotation transforms factors to physically meaningful spectral profiles. Alternating least squares optimised the profiles. 3. RESULTS Laser induced fluorescence spectra were obtained from sixty samples. In Figure 1, the normalized spectra are presented. 241 Proc SPIE Vol. 4903
3 Fig. 1. Normalized fluorescence spectra. Principal component analysis was used to derive principal components - basis spectra responsible for the spectral variation. Derived principal components were tested with the Malinowski s indicator function [4] to select statistically significant principal components - factors. Remaining principal components after factor selection describes only noise and they are discarded from further analysis. Seven factors were found to be statistically significant. In Figure 2, the first three principal components - factors are shown. Fig. 2. First three principal components - Factors considered as basis spectra characterizing main spectral changes in the data set. In Figure 3, seventh and eighth principal components are shown. The eighth and further principal components describe only noise. These last principal components are discarded and not included in the further analysis. Proc. SPIE Vol
4 Fig. 3. Seven principal components are considered. The eighth and further principal components describe only noise. These last principal components are discarded and seven principal components factors are left for further analysis The factors are not directly related to any real chemical spectra (they, for example, are negative). Thus, non-orthogonal oblique rotation (SYSTAT 10, SPSS) was used to rotate factors. In Figure 4, seven rotated factors are shown. The rotation has no unique solution in attempt to resolve spectral profiles. Fig. 4. Spectra derived by factors non-orthogonal oblique rotation. Ambiguities presented in recovering profiles using factor rotation are solved by use of iterative optimisation methods and of chemical constraints. Alternating least squares algorithm with codes written in MatLab (MatLab version 6, Mathworks) was used for the optimisation. The rotated factors were used as initial estimates and non-negativity of spectra and concentrations, unimodality and linear additivity of spectra were assumed as chemical constraints. In Figure 5, resolved spectral profiles by multivariate curve resolution are shown. 243 Proc SPIE Vol. 4903
5 Fig. 5. Spectra derived by multivariate curve resolution. An attempt might be made to identify resolved spectral profiles (Fig. 5). However curve resolution is restricted to spectra overlapping. Resolved spectra can be compared to fluorophores spectra (Fig. 6) used in our previous work [6]. Fig. 6. Spectra of collagen, elastin, neopterin, NADH, Vitamins, Caroten an porphyrins respectively, found elsewhere, derived from spectra or measured during the research (neopterin). Multivariate curve resolution was used to resolve concentration profiles as well. Average concentrations of resolved spectral components are shown by the average of spectra intensity in Fig. 7. No one of the pure spectra (Fig. 5) resolved by multivariate curve resolution from fluorescence data (Fig. 1) was identified as a neopterin spectrum (Fig. 6). However, there is an intensity increase in the neopterin spectra spectral region (about nm) in hyperplastic endometrial samples. Proc. SPIE Vol
6 Fig 7. Average concentrations of resolved spectral components are shown by the average of spectra intensity. 4. CONCLUSION AND SUMMARY Seven factors fluorescence of natural fluorophores of endometrial tissue were found to be present above the noise level in the overall autofluorescence spectra. The seven pure spectra obtained by multivariate curve resolution can be used to identify chemical species using library spectra. Neopterin concentration may be too low in endometrial tissue to make its fluorescence above the noise level because neopterin spectrum was not found to be among the spectra resolved by multivariate curve resolution. There was observed an intensity increase in the neopterin spectrum spectral region (about nm) in hyperplastic endometrial samples which might be associated with neopterin concentration increase but the changes are subtle and are not statistically significant. REFERENCE 1. R. Richards-Kortum, E. Sevick-Muraca, Quantitative Optical Spectroscopy for Tissue Diagnosis, Annu. Rev. Phys. Chem., 47, (1996) 2. D. Fuchs, G. Weiss, H. Wachter, Neopterin, biochemistry and clinical use as a marker for cellular immune reactions. Int Arch Allergy Immunol 101:1-6 (1993) 3. E. Casassas, I. Marqus, R. Tauler. Study of acid-base properties of fulvic acids using fluorescencespectrometry and multivariate curve resolution methods. Anal. Chem. Act. 45: (1995) 4. R. Tauler, B.R. Kowalski and S. Flemming. Multivariate curve resolution applied to spectral data from multiple runs of an industrial process. Anal. Chem., (1993) 5. E.R. Malinowski, Factor Analysis in Chemistry, 2nd ed., Wiley, Chichester, V. Gavryushin, J. Vaitkus, A. Vaitkuviene. Methodology of medical diagnostics of human tissue by fluorescence. Lithuanian J. Physics, Vol. 40, No.4, (2000) 245 Proc SPIE Vol. 4903
Laser- and Light-Induced Autofluorescence Spectroscopy of Human Skin in Dependence on Excitation Wavelengths
Vol. 112 (2007) ACTA PHYSICA POLONICA A No. 5 Proceedings of the International School and Conference on Optics and Optical Materials, ISCOM07, Belgrade, Serbia, September 3 7, 2007 Laser- and Light-Induced
More informationExperts in Femtosecond Laser Technology. DermaInspect. Non-invasive multiphoton tomography of human skin
Experts in Femtosecond Laser Technology DermaInspect Non-invasive multiphoton tomography of human skin In vivo optical biopsies with subcellular spatial resolution based on near infrared femtosecond laser
More informationTITLE: Fluorescence and Diffuse Reflectance Spectroscopy for Breast Cancer Diagnosis During Core Needle Biopsy
AD AWARD NUMBER: W81XWH-05-1-0380 TITLE: Fluorescence and Diffuse Reflectance Spectroscopy for Breast Cancer Diagnosis During Core Needle Biopsy PRINCIPAL INVESTIGATOR: Changfang Zhu CONTRACTING ORGANIZATION:
More information1st Faculty of Medicine, Charles University in Prague Center for Advanced Preclinical Imaging (CAPI)
ADVANTAGES Optical Imaging OI Optical Imaging is based on the detection of weak light by a highly sensitive and high resolution CCD camera DISADVANTAGES High sensitivity Limited penetration depth Easy
More informationFluorescence spectroscopy of oral tissue: Monte Carlo modeling with site-specific tissue properties
Journal of Biomedical Optics 141, 014009 January/February 2009 Fluorescence spectroscopy of oral tissue: Monte Carlo modeling with site-specific tissue properties Ina Pavlova The University of Texas at
More informationMultiexcitation Fluorescence and Reflectance Spectroscopy as a Real Time Guide to Biopsy in Vivo
Multiexcitation Fluorescence and Reflectance Spectroscopy as a Real Time Guide to Biopsy in Vivo Markus G. MUller*a, Irene Georgakoudi', Adam Waxa, Ramachandra Dasari', Christian Jostb, Michael Wallace',
More informationSpecial Techniques 1. Mark Scott FILM Facility
Special Techniques 1 Mark Scott FILM Facility SPECIAL TECHNIQUES Multi-photon microscopy Second Harmonic Generation FRAP FRET FLIM In-vivo imaging TWO-PHOTON MICROSCOPY Alternative to confocal and deconvolution
More informationQuantitative Characterization of Biological Tissue Using Optical Spectroscopy
Irene Georgakoudi Massachusetts Institute of Technology Cambridge, Massachusetts Jason T. Motz Massachusetts Institute of Technology Cambridge, Massachusetts Vadim Backman Northwestern University Evanston,
More informationCompensation: Fundamental Principles
Flow Cytometry Seminar Series 2017 : Fundamental Principles Spillover correction in multicolor flow cytometry 28.02.2017 http://www.cytometry.uzh.ch Contents Fluorescence and its detection Absorption and
More informationSpectroscopy and Imaging IV
PROGRESS IN BIOMEDICAL OPTICS AND IMAGING Vol. 16 No. 55 Clinical and Biomedical Spectroscopy and Imaging IV J. Quincy Brown Volker Decked Edifors 22-24 June 2015 Munich, Germany Sponsored by SPIE (United
More informationDesign for Manufacturability (DFM) in the Life Sciences
T E C H N I C A L N O T E Design for Manufacturability (DFM) in the Life Sciences Fluorescence Spectroscopy Product Platform Realized with TracePro TM Suite of Opto-Mechanical Design Software Tools Authors:
More informationMultispectral Fluorescence Imaging. Laboratory exercise. Multi-spectral imaging
Multispectral Fluorescence Imaging Laboratory exercise Multi-spectral imaging Lab supervisors: Arefeh Mousavi (arefeh.mousavi@fysik.lth.se) Yu-Chen Cheng (yu-chen.cheng@fysik.lth.se) Laboratory room: A306
More informationTrimodal spectroscopy for the detection and characterization of cervical precancers in vivo
Trimodal spectroscopy for the detection and characterization of cervical precancers in vivo Irene Georgakoudi, PhD, a Ellen E. Sheets, MD, b Markus G. Müller, MSc, a Vadim Backman, PhD, a Christopher P.
More informationContact Details. Dr Alexander Galkin. Office: MBC Room 186. Tel: (028) Frequency and wavelength.
Contact Details The electromagnetic spectrum Biological Spectroscopy Dr Alexander Galkin Email: a.galkin@qub.ac.uk Dr Alexander Galkin MSc Biomolecular Function - BBC8045 Office: MBC Room 186 Tel: (028)
More informationFluorescence spectroscopy coupled with factorial discriminant analysis technique to identify sheep milk from different feeding systems.
Fluorescence spectroscopy coupled with factorial discriminant analysis technique to identify sheep milk from different feeding systems. HAMMAMI Moncef, Ph.D Associate professor Higher School of Agriculture
More informationMicroendoscopes for imaging of the pancreas
Microendoscopes for imaging of the pancreas Angelique Kano *, Andrew R. Rouse, Shona M. Kroto, Arthur F. Gmitro Optical Sciences Center& Radiology Research Lab, University of Arizona, Tucson 85724 ABSTRACT
More informationThe analysis of fluorescence microscopy images for FRET detection
The analysis of fluorescence microscopy images for FRET detection Ela Claridge, Dale J. Powner and Michael J.O. Wakelam School of Computer Science, The University of Birmingham B5 2TT Institute for Cancer
More informationAutofluorescence Microscopy of Fresh Cervical-Tissue Sections Reveals Alterations in Tissue Biochemistry with Dysplasia
Photochemistry and Photobiology, 2001, 73(6): 636 641 Autofluorescence Microscopy of Fresh Cervical-Tissue Sections Reveals Alterations in Tissue Biochemistry with Dysplasia Rebekah Drezek 1, Carrie Brookner
More informationNUMEROUS studies have demonstrated that techniques
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, VOL. 46, NO. 11, NOVEMBER 1999 1293 Performance Estimation of Diagnostic Tests for Cervical Precancer Based on Fluorescence Spectroscopy: Effects of Tissue
More informationUrs Utzinger, E. Vanessa Trujillo, E. Neely Atkinson, Michele F. Mitchell, Scott B. Cantor, and Rebecca Richards-Kortum* /99$10.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, VOL. 46, NO. 11, NOVEMBER 1999 1293 Performance Estimation of Diagnostic Tests for Cervical Precancer Based on Fluorescence Spectroscopy: Effects of Tissue
More informationComponents for Highly Advanced time-resolved fluorescence Microscopy based on Nonlinear Glass fibres
Components for Highly Advanced time-resolved fluorescence Microscopy based on Nonlinear Glass fibres FP7 Call identifier: FP7-ICT-2011-7 Activity code: ICT-2011.3.5: Photonics Project start date and duration:
More informationF* techniques: FRAP, FLIP, FRET, FLIM,
F* techniques: FRAP, FLIP, FRET, FLIM, FCS Antonia Göhler March 2015 Fluorescence explained in the Bohr model Absorption of light (blue) causes an electron to move to a higher energy orbit. After a particular
More informationDirectional Surface Plasmon Coupled Emission
Journal of Fluorescence, Vol. 14, No. 1, January 2004 ( 2004) Fluorescence News Directional Surface Plasmon Coupled Emission KEY WORDS: Surface plasmon coupled emission; high sensitivity detection; reduced
More informationStatistically Integrated Metabonomic-Proteomic Studies on a Human Prostate Cancer Xenograft Model in Mice
Statistically Integrated Metabonomic-Proteomic Studies on a Human Prostate Cancer Xenograft Model in Mice Mattias Rantalainen, Olivier Cloarec, Olaf Beckonert, I. D. Wilson, David Jackson, Robert Tonge,
More informationAnthocyanin degradation of Hibiscus sabdariffa extracts during storage monitored by MCR-ALS on UV-VIS spectra
Anthocyanin degradation of Hibiscus sabdariffa extracts during storage monitored by MCR-ALS on UV-VIS spectra N. Achir 1, C. Mertz 2, A. Sinela 3, K. Vidot 2, H. Fulcrand 4, M. Dornier 1 1 Montpellier
More informationConfocal Microscopy Analyzes Cells
Choosing Filters for Fluorescence A Laurin Publication Photonic Solutions for Biotechnology and Medicine November 2002 Confocal Microscopy Analyzes Cells Reprinted from the November 2002 issue of Biophotonics
More informationOral cancer is the eighth most frequently diagnosed type of cancer in the world.
Oral cancer is the eighth most frequently diagnosed type of cancer in the world. According to the American Cancer Society, every hour one citizen of America dies from oral and throat cancer. In 2008, this
More informationHow to Build the World s Fastest Spectrofluorometer. MF 2 Multi-Frequency Fluorometer
How to Build the World s Fastest Spectrofluorometer MF 2 Multi-Frequency Fluorometer Time is always on your side with a HORIBA Jobin Yvon MF 2 (Multi-Frequency Fluorometer) lifetime spectrofluorometer.
More informationBiophotonics II general remarks
general remarks BIOPHOTONICS I (WS 2017/18) I. Imaging Systems human vision microscopy II. Light Scattering Mie scattering light propagation in tissue BIOPHOTONICS II (SS 2018) III. Biospectroscopy Fluorescence
More informationMicrochip Electrophoresis for Glycoprotein Separation. Analytical Strategy, September 30th, 2014 Karina Hasler & Adrian Müller
Microchip Electrophoresis for Glycoprotein Separation Analytical Strategy, September 30th, 2014 Karina Hasler & Adrian Müller Karina Hasler & Adrian Müller 30.09.2014 1 Content Glycoproteins Available
More informationQuantitative Proteomics: From Technology to Cancer Biology
Quantitative Proteomics: From Technology to Cancer Biology Beyond the Genetic Prescription Pad: Personalizing Cancer Medicine in 2014 February 10-11, 2014 Thomas Kislinger Molecular Biomarkers in Body
More informationWidefield Microscopy Bleed-Through
In widefield microscopy the excitation wavelengths which illuminate the sample, and the emission wavelengths which reach the CCD camera are selected throughout a filter cube. A filter cube consists of
More informationFluorescence Imaging with Medical Applications
Fluorescence Imaging with Medical Applications LABORATORY EXERCISE MULTI-SPECTRAL IMAGING Lab supervisors: Anne Harth (anne.harth@fysik.lth.se) Haichun Liu (haichun.liu@fysik.lth.se) Laboratory room: A207
More informationUsing fluorescence for rapid wastewater presence detection in sources of drinking water
Using fluorescence for rapid wastewater presence detection in sources of drinking water Nicolas Peleato, Robert C. Andrews, Ray L. Legge* Department of Civil Engineering, University of Toronto and *Department
More informationTowards Probing Skin Cancer using Endogenous Melanin Fluorescence
8 Towards Probing Skin Cancer using Endogenous Melanin Fluorescence Andra Colbert, McNair Scholar, Virginia State University Faculty Research Advisor: Dr. Ahmed A. Heikal Associate Professor of Bioengineering
More informationSECOND DERIVATIVE UV FOR RAPID CONFORMATIONAL ASSESSMENT OF PROTEINS
SECOND DERIVATIVE UV FOR RAPID CONFORMATIONAL ASSESSMENT OF PROTEINS NOVEMBER 2016 KEVIN MCCOWEN SUPERVISOR ANALYTICAL DEVELOPMENT OVERVIEW Brief overview of UV absorbance Development of statistical analysis
More informationMultiplexed 3D FRET imaging in deep tissue of live embryos Ming Zhao, Xiaoyang Wan, Yu Li, Weibin Zhou and Leilei Peng
Scientific Reports Multiplexed 3D FRET imaging in deep tissue of live embryos Ming Zhao, Xiaoyang Wan, Yu Li, Weibin Zhou and Leilei Peng 1 Supplementary figures and notes Supplementary Figure S1 Volumetric
More informationBiomedical Applications of Molecular Spectroscopy
Biomedical Applications of Molecular Spectroscopy Mike Kayat B&W Tek, Inc 19 Shea Way Newark, DE 19713 United States of America +1 302 368 7824 mikek@bwtek.com 1 Overview Molecular spectroscopy is a large
More informationfor MA and Beyond John Gabrielson CASSS 2 nd International Symposium on Higher Order Structure of Protein Therapeutics
Higher Order Structure Characterization for MA and Beyond John Gabrielson CASSS 2 nd International Symposium on Higher Order Structure of Protein Therapeutics February 12, 2013 Proteins form multiple levels
More informationNeed to identify cell subpopulations
Problem Hyperspectral microscopy imaging Our methods Key results Conclusion Acknowledgements Need to identify cell subpopulations Biological cells are very heterogeneous. Significant major subpopulations
More informationLaser-induced Fluorescence (LIF) Based Optical Fiber Probe for Biomedical Application
Laser-induced Fluorescence (LIF) Based Optical Fiber Probe for Biomedical Application Jagdish P. Singh, Sunil K. Khijwania, Chan Kyu Kim, Fang-Yu Yueh Shane C. Burgess, Diagnostic Instrumentation and Analysis
More informationLow temperature fluorescence imaging of freezetrapped human cervical tissues
Low temperature fluorescence imaging of freezetrapped human cervical tissues Nirmala Ramanujam Department of Biomedical Engineering, University of Wisconsin, Madison, WI Rebecca Richards-Kortum, Sharon
More informationSupporting On-line Materials
Supporting On-line Materials Conformational Analysis of DNA Repair Intermediates by Time-Resolved Fluorescence Spectroscopy Su Lin 1, David P. Horning 2, Jack W. Szostak 2, and John C. Chaput* 1 1 Center
More informationOptimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy
Photochemistry and Photobiology,, 7(): Optimal Excitation Wavelengths for In Vivo Detection of Oral Neoplasia Using Fluorescence Spectroscopy Douglas L. Heintzelman, Urs Utzinger, Holger Fuchs, Andres
More informationBackground and Objective: The hamster cheek pouch carcinogenesis model, using chronic treatments of dimethylbenz[a]anthracene
Lasers in Surgery and Medicine 29:1±10 (2001) Fluorescence Spectroscopy of Epithelial Tissue Throughout the Dysplasia-Carcinoma Sequence in an Animal Model: Spectroscopic Changes Precede Morphologic Changes
More informationPrinciples of flow cytometry: overview of flow cytometry and its uses for cell analysis and sorting. Shoreline Community College BIOL 288
Principles of flow cytometry: overview of flow cytometry and its uses for cell analysis and sorting Shoreline Community College BIOL 288 Flow Cytometry What is Flow Cytometry? Measurement of cells or particles
More informationProteomics And Cancer Biomarker Discovery. Dr. Zahid Khan Institute of chemical Sciences (ICS) University of Peshawar. Overview. Cancer.
Proteomics And Cancer Biomarker Discovery Dr. Zahid Khan Institute of chemical Sciences (ICS) University of Peshawar Overview Proteomics Cancer Aims Tools Data Base search Challenges Summary 1 Overview
More informationAn automated image processing routine for segmentation of cell cytoplasms in high-resolution autofluorescence images
An automated image processing routine for segmentation of cell cytoplasms in high-resolution autofluorescence images Alex J. Walsh a, Melissa C. Skala *a a Department of Biomedical Engineering, Vanderbilt
More informationOverview of Immunohistochemistry
Overview of Immunohistochemistry Immunohistochemistry (IHC) combines anatomical, immunological and biochemical techniques to identify discrete tissue components by the interaction of target antigens with
More informationJournal of Environmental Sciences 19(2007)
Journal of Environmental Sciences 19(2007) 787 791 Composition analysis of colored dissolved organic matter in Taihu Lake based on three dimension excitation-emission fluorescence matrix and PARAFAC model,
More informationState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing , China
2017 International Conference on Medicine Sciences and Bioengineering (ICMSB 2017) ISBN: 978-1-60595-508-7 Raman Imaging of the Distribution of Carotenoids in Haematococcus Pluvialis under High Concentration
More informationLaser Induced Fluorescence Spectroscopy and Molecular Imaging as Tools for Tumor Detection in vivo
Laser Induced Fluorescence Spectroscopy and Molecular Imaging as Tools for Tumor Detection in vivo B. Ebert Physikalisch-Techn. Bundesanstalt, Section 8.31, PTB, Berlin, Germany Outline: Spectroscopic
More informationPhotoacoustic Imaging in Biomedicine Critical Review by Saurabh Vyas Group 9: Interventional Photoacoustic Ultrasound CIS II: 600.
Photoacoustic Imaging in Biomedicine Critical Review by Saurabh Vyas Group 9: Interventional Photoacoustic Ultrasound CIS II: 600.446, Spring 2011 Introduction Photoacoustic imaging (PA Imaging) is the
More informationBIOINFORMATICS THE MACHINE LEARNING APPROACH
88 Proceedings of the 4 th International Conference on Informatics and Information Technology BIOINFORMATICS THE MACHINE LEARNING APPROACH A. Madevska-Bogdanova Inst, Informatics, Fac. Natural Sc. and
More informationDrug Discovery Research Clinical Screening. Comparison of ELISA and AlphaScreen Assay Technologies for Measurement of Protein Expression Levels
Drug Discovery Research Clinical Screening FLAG Expression Measurement Application Note Comparison of ELISA and AlphaScreen Assay Technologies for Measurement of Protein Expression Levels ALPHASCREEN APPLICATION
More informationDENATURATION OF HEMOGLOBIN IN THE PRESENCE OF TANNIC ACID
PROCEEDINGS OF THE YEREVAN STATE UNIVERSITY C h e m i s t r y a n d B i o l o g y 2014, 1, p. 23 27 C h emistr y DENATURATION OF HEMOGLOBIN IN THE PRESENCE OF TANNIC ACID K. R. GRIGORYAN, L. S. SARGSYAN
More informationFluorescence Intrinsic Characterization of Excitation-Emission Matrix Using Multi-Dimensional Ensemble Empirical Mode Decomposition
Int. J. Mol. Sci. 2013, 14, 22436-22448; doi:10.3390/ijms141122436 Article OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 www.mdpi.com/journal/ijms Fluorescence Intrinsic Characterization
More informationOur Solutions for Protein Analysis
Our Solutions for Protein Analysis www.horibalifesciences.com info.sci@horiba.com Solutions for Protein Characterization Kinetics Proteins are involved in many critical roles in the body: Enzymes - allow
More informationLASER INDUCED FLUORESCENCE EFFICIENCY IN WATER QUALITY ASSESSMENT
Romanian Reports in Physics, Vol. 62, No. 3, P. 652 659, 2010 Dedicated to the 50 th LASER Anniversary (LASERFEST-50) LASER INDUCED FLUORESCENCE EFFICIENCY IN WATER QUALITY ASSESSMENT L. GHERVASE, E.M.
More informationTranslational Multimodality Optical Imaging
Translational Multimodality Optical Imaging Fred S. Azar Xavier Intes Editors 0 ARTECH H O U S E BOSTON LONDON artechhouse.com Contents Foreword Preface xv xvii CHAPTER1 Introduction to Clinical Optical
More informationExploring metallic nanoparticles in Biophotonics
Exploring metallic nanoparticles in Biophotonics Renato E. de Araujo Laboratório de Óptica Biomédica e Imagem, Universidade Federal de Pernambuco Recife, PE, Brazil. renato.earaujo@ufpe.br LABORATÓRIO
More informationWelcome! openmicberkeley.wordpress.com. Open Berkeley
Welcome! openmicberkeley.wordpress.com Agenda Jen Lee: Introduction to FRET Marla Feller: Using FRET sensors to look at time resolved measurements Becky Lamason: Using FRET to determine if a bacterial
More informationMEDICAL PHYSICS (MED PHYS)
Medical Physics (MED PHYS) 1 MEDICAL PHYSICS (MED PHYS) MED PHYS/PHYSICS 265 INTRODUCTION TO MEDICAL PHYSICS Primarily for premeds and other students in the medical and biological sciences. Applications
More informationTime resolved fluorescence spectroscopy: An old technique to monitor protein higher order structure changes
Time resolved fluorescence spectroscopy: An old technique to monitor protein higher order structure changes Sergey Arzhantsev Division of Pharmaceutical Analysis, Office of Testing and Research Center
More information3.1.4 DNA Microarray Technology
3.1.4 DNA Microarray Technology Scientists have discovered that one of the differences between healthy and cancer is which genes are turned on in each. Scientists can compare the gene expression patterns
More informationDevelopment of the Autofluorescence Endoscope Imaging System
Diagnostic and Therapeutic Endoscopy, Vol. 5, pp. 65-70 Reprints available directly from the publisher Photocopying permitted by license only (C) 1999 OPA (Overseas Publishers Association) N.V. Published
More informationDETECTION OF ATHEROSCLEROTIC CORONARY PLAQUES BY FLUORESCENCE LIFETIME IMAGING ANGIOSCOPY. A Thesis PATRICK ALLEN THOMAS
DETECTION OF ATHEROSCLEROTIC CORONARY PLAQUES BY FLUORESCENCE LIFETIME IMAGING ANGIOSCOPY A Thesis by PATRICK ALLEN THOMAS Submitted to the Office of Graduate Studies of Texas A&M University in partial
More informationLUPAS Luminescent Polymers for in vivo Imaging of Amyloid Signatures
LUPAS Luminescent Polymers for in vivo Imaging of Amyloid Signatures A research project for innovative diagnostics for neurodegenerative disorders Funded by the European Union under the 7 th Framework
More informationSapphire. Biomolecular Imager THE NEXT GENERATION OF LASER-BASED IMAGING
Sapphire Biomolecular Imager THE NEXT GENERATION OF LASER-BASED IMAGING Breakthrough image capture and analysis The Sapphire Biomolecular Imager is a next generation laser scanning system that provides
More informationBasic principles of NMR-based metabolomics
Basic principles of NMR-based metabolomics Professor Dan Stærk Bioanalytical Chemistry and Metabolomics research group Natural Products and Peptides research section Department of Drug Design and Pharmacology
More informationEstablishment and verification of mirnas expression molecules for breast cancer and its distant metastasis Yanhong Gao Associate Professor
Establishment and verification of mirnas expression molecules for breast cancer and its distant metastasis Yanhong Gao Associate Professor Department of Clinical Biochemistry Chinese PLA General Hospital
More informationRaising the Bar in Preclinical Imaging
1 sur 5 30/03/2016 11:19 Send to printer» Feature Articles : Nov 1, 2013 (Vol. 33, No. 19) Raising the Bar in Preclinical Imaging MaryAnn Labant Preclinical imaging focuses predominantly on estimating
More informationModel-based spectroscopic analysis of the oral cavity: impact of anatomy
Journal of Biomedical Optics 136, 064034 November/December 2008 Model-based spectroscopic analysis of the oral cavity: impact of anatomy Sasha McGee Jelena Mirkovic Massachusetts Institute of Technology
More informationNanoPro 1000 System. characterize cell signaling in your smallest samples
NanoPro 1000 System characterize cell signaling in your smallest samples Technology The NanoPro 1000 system is a capillary-based nano-immunoassay platform. As in Western blot analysis, proteins from complex
More informationChapter-IV OPTICAL PROPERTIES
Chapter-IV OPTICAL PROPERTIES 4.1 Ultraviolet/ Visible Spectroscopy UV- visible spectroscopy of wavelength is shorter than visible light, but longer than X- rays. UV-Violet color is the shortest wavelength
More informationTime-resolved Measurements Using the Agilent Cary Eclipse Fluorescence Spectrophotometer A Versatile Instrument for Accurate Measurements
Time-resolved Measurements Using the Agilent Cary Eclipse Fluorescence Spectrophotometer A Versatile Instrument for Accurate Measurements Technical Overview Authors Dr. Fabian Zieschang, Katherine MacNamara,
More informationQUANTITATIVE DETERMINATION OF HUMAN EPIDIDYMIS PROTEIN 4
QUANTITATIVE DETERMINATION OF HUMAN EPIDIDYMIS PROTEIN 4 NEW PRODUCT Human Epididymis Protein 4 () ELISA High sensitivity (0.15 pmol/l) Excellent analytical characteristics Validated for human serum samples,
More informationSUPPLEMENTARY FIGURES
SYNERGISTIC STRATEGY FOR MULTICOLOR TWO-PHOTON MICROSCOPY: APPLICATION TO THE ANALYSIS OF GERMINAL CENTER REACTIONS IN VIVO ASYLKHAN RAKHYMZHAN, RUTH LEBEN, HANNA ZIMMERMANN, ROBERT GÜNTHER, PEGGY MEX,
More informationIntrinsic fluorescence spectroscopy in turbid media: disentangling effects of scattering and absorption
Intrinsic fluorescence spectroscopy in turbid media: disentangling effects of scattering and absorption Markus G. Müller, Irene Georgakoudi, Qingguo Zhang, Jun Wu, and Michael S. Feld The fluorescence
More informationREPEATABILITY, REPRODUCIBILITY AND ANALYTIC STANDARDS FOR BIOMARKER DEVELOPMENT
REPEATABILITY, REPRODUCIBILITY AND ANALYTIC STANDARDS FOR BIOMARKER DEVELOPMENT ABBAS BANDUKWALA BIOMARKER QUALIFICATION PROGRAM ABBAS.BANDUKWALA@FDA.HHS.GOV May 17, 2018 1 Disclaimer This presentation
More informationTissue temperature measurements during interstitial laser therapy using Cr3+-doped crystals at the fiber tip
Tissue temperature measurements during interstitial laser therapy using Cr3+-doped crystals at the fiber tip Svensson, Jenny; Ralsgard, Anna; Johansson, Thomas; Andersson-Engels, Stefan Published in: Proceedings
More informationLaser-Induced Breakdown Spectroscopy (Libs) in Civil Engineering
ECNDT 2006 - Poster 15 Laser-Induced Breakdown Spectroscopy (Libs) in Civil Engineering Alexander TAFFE, Dieter SCHAURICH, Friederike WERITZ, Gerd WILSCH, Bundesanstalt für Materialforschung und -prüfung,
More informationSPECTRAL STUDY ON THE FORMATION OF QUANTUM DOTS AND CHLORIN E6 COMPLEX IN THE PRESENCE OF PROTEIN
SPECTRAL STUDY ON THE FORMATION OF QUANTUM DOTS AND CHLORIN E6 COMPLEX IN THE PRESENCE OF PROTEIN Artiom SKRIPKA 1, Jurga VALANČIŪNAITĖ 2, Ričardas ROTOMSKIS 1, 2 1 Vilnius University Physics faculty,
More informationCapabilities & Services
Capabilities & Services Accelerating Research & Development Table of Contents Introduction to DHMRI 3 Services and Capabilites: Genomics 4 Proteomics & Protein Characterization 5 Metabolomics 6 In Vitro
More informationSensoLyte Plus 520 MMP-1 Assay Kit *Fluorimetric and Enhanced Selectivity*
SensoLyte Plus 520 MMP-1 Assay Kit *Fluorimetric and Enhanced Selectivity* Catalog # 72012 Kit Size 96 Assays in 96-well plate Optimized Performance: Optimal conditions for detecting MMP-1 activity in
More informationMeasurement and Calibration of near- and mid- infrared Spectroscopy to predict the Biomass Composition for optimal Pretreatment
Measurement and Calibration of near- and mid- infrared Spectroscopy to predict the Biomass Composition for optimal Pretreatment H. Wulfhorst, M. Flüggen, S. Maurer, S. Möhring, J. Roth, N.Tippkötter Bioprocess
More informationCancer Research. Peptide Innovations in. Neo-Epitope Analysis Immunotherapy Cancer Proteomics Enzyme Profiling Epigenetics
Peptide Innovations in Cancer Research Neo-Epitope Analysis Immunotherapy Cancer Proteomics Enzyme Profiling Epigenetics Innovative Peptide Solutions Neo-Epitope Analysis Neo-epitopes encoded by the mutanome
More informationAbsorption of an electromagnetic wave
In vivo optical imaging?? Absorption of an electromagnetic wave Tissue absorption spectrum Extinction = Absorption + Scattering Absorption of an electromagnetic wave Scattering of an electromagnetic wave
More information2.3 Quantum Dots (QDs)
2.3 Quantum Dots (QDs) QDs are inorganic nanocrystals, approximately 1 10 nm in size, with unique optical properties of broad excitation, narrow size-tunable emission spectra, high photochemical stability,
More informationTWO-PHOTON EXCITED FLUORESCENCE OF THE LENS FOR THE DIAGNOSIS OF PRESBYOPIA
ОПТИКА И СПЕКТРОСКОПИЯ, 29, том 17, 3, с. 495 499 УДК 621.373:375 НАНОФОТОНИКА И БИОМЕДИЦИНА TWO-PHOTON EXCITED FLUORESCENCE OF THE LENS FOR THE DIAGNOSIS OF PRESBYOPIA 29 г. R. Steiner, M. Kessler, O.
More informationPre-Clinical Optical Molecular Imager
Pre-Clinical Optical Molecular Imager ART Advanced Research Technologies Inc. 2300 Alfred-Nobel Blvd. Saint-Laurent, QC Canada, H4S 2A4 T 514.832.0777 / 1.888.278.7888 F 514.832.0778 E info@art.ca Table
More informationDeliverable 2.1: Definition of paradigms representing exemplary breast lesions cases
Project title: Smart Optical and Ultrasound Diagnostics of Breast Cancer Grant Agreement: 731877 Call identifier: H2020-ICT-2016-1 Topic: ICT-29-2016 Photonics KET 2016 Deliverable 2.1: Definition of paradigms
More informationMetabolomics S.M.Shotorbani, V. A. Suliman
13 International Journal of Advance Research, IJOAR.org Volume 1, Issue 1, January 2013, Online: Metabolomics S.M.Shotorbani, V. A. Suliman ABSTRACT A brief review on metabolomics, a new field being explored,
More informationNanoPro Characterize cell signaling in your smallest samples
NanoPro 1000 Characterize cell signaling in your smallest samples Technology A NEW TAKE ON IMMUNOASSAYS The NanoPro 1000 is a capillary-based nano-immunoassay platform. As in Western blot analysis, proteins
More informationLecture 13: Analysis of 2D gels
Lecture 13: Analysis of 2D gels A complete proteomic analysis aims at collecting quantitative information about all protein in a sample. A normal 2 Dimensional gel electrophoresis results are analyzed
More informationAlkaline Phosphatase Fluorimetric Assay Kit
Alkaline Phosphatase Fluorimetric Assay Kit Catalog No. KM0019 Detection and Quantification of Alkaline Phosphatase Concentrations in Biological Samples. Research Purposes Only. Not Intended for Diagnostic
More informationReceived 22 January 2007; Revised 16 April 2007; Accepted 18 April 2007
QUALITY AND RELIABILITY ENGINEERING INTERNATIONAL Qual. Reliab. Engng. Int. (in press) Published online in Wiley InterScience (www.interscience.wiley.com)..862 Special Issue Analysing Multivariate Data
More informationBiochemical Imaging of Human Atherosclerotic Plaques with Fluorescence Lifetime Angioscopy
Photochemistry and Photobiology, 2010, 86: 727 731 Research Note Biochemical Imaging of Human Atherosclerotic Plaques with Fluorescence Lifetime Angioscopy Patrick Thomas 1, Paritosh Pande 1, Fred Clubb
More informationChapter 4 Fluorescence Resonance Energy Transfer (FRET) by Minor Groove-Associated Cyanine-Polyamide Conjugates
Chapter 4 Fluorescence Resonance Energy Transfer (FRET) by Minor Groove-Associated Cyanine-Polyamide Conjugates The work described in this chapter was accomplished in collaboration with V. Rucker (Dervan
More informationBiophotonics II general remarks
general remarks BIOPHOTONICS I (WS 2017/18) I. Imaging Systems human vision microscopy II. Light Scattering Mie scattering light propagation in tissue BIOPHOTONICS II (SS 2018) III. Biospectroscopy Fluorescence
More information