Thermo Scientific Transcend System

Transcription

1 m a s s s p e c t r o m e t r y Thermo Scientific Transcend System Increase the Efficiency of Your Mass Spectrometry Workflow Bioanalytical Assays Clinical Research and Forensic Toxicology Food Safety Equine Doping Part of Thermo Fisher Scientific

2 TurboFlow technology a superior online sample extraction technique for complex matrices for the mass spectrometer TurboFlow Technology TurboFlow technology is an innoatie approach to sample preparation based on chromatographic principles. This online, automated process combines principles of diffusion, chemistry and size exclusion to quickly eliminate matrix interferences while capturing analytes of interest. The ability to analyze complex samples with high sensitiity using minimal preparation is the direct result of TurboFlow technology. Labs around the world are simplifying their methods by performing sample preparation and liquid chromatography together in one step. TurboFlow technology gies scientists greater confidence in their LC-MS/MS results. Minimizes sample preparation inject samples directly into LC/MS system Reduces ion suppression through higher specificity Saes time simplifies complex sample preparation protocols Simplifies method deelopment use the same method for different matrices Multiplexing Technology The unique Thermo Scientific multiplexing technology brings the productiity of up to four separate, parallel LC systems to a single mass spectrometer. With Aria operating software each channel operates independently, so you can run a single method or multiple methods simultaneously. This critical feature improes MS efficiency, unlike traditional single-channel LC systems whose detectors are idle more than 75 percent of the time. The Transcend system ensures the maximum utilization of your mass spectrometer with irtually no idle time. Sae money effortlessly and boost productiity, without compromising data quality or sensitiity. Accelerates results quadruple mass spec throughput Enhances productiity analyze more samples per hour Improes efficiency mass spec idle less than 4 percent of the time Increases flexibility run up to four different assays at the same time Transcend system unique multiplexing capability 4 Min Meaningful Data Traditional LC/MS systems are idle 75% of the time. CTC Auto Sampler LC Pumps LC Pumps LC Pumps LC Pumps The MS/MS idle for <4% of the time Parallel System, Serial Data Column Column Column Column MS/MS Detector Multiplexed system with four independent, parallel channels. Sample injections and acquisitions are staggered among four HPLC systems in an intelligent manner with Aria Software Transcend maximizes your MS, permitting 4 different methods to run simultaneously. Liquid-Liquid Extraction (LLE) Solid Phase Extraction (SPE) Protein Precipitation (PPT) TurboFlow Method 1. Aliquot of sample 2. Spike with IS 3. Add buffer 4. Add MTBE 5. Shake 1 min 6. Centrifuge 7. Remoe organic 8. Eaporate to dryness 9. Reconstitute 1. Transfer to plate 11. Inject onto column 1. Aliquot of sample 2. Spike with IS 3. Add.1N HCL 4. Condition sorbent 5. Add sample to sorbent 6. Wash 7. Eaporate 8. Reconstitute 9. Transfer 1. Inject onto column 1. Aliquot of sample 2. Spike with IS 3. Add acetonitrile 4. Centrifuge 5. Remoe supernatant 6. Reconstitute 7. Transfer to plate 8. Inject onto column 1. Aliquot of sample 2. Spike with IS 3. Centrifuge 4. Inject onto column

3 Combine the power of online extraction with the speed of UHPLC Transcend System The Thermo Scientific Transcend system is the most productie liquid chromatography (LC) solution aailable the only truly independent, parallel, multi-channel UHPLC system in the industry. The Transcend system uses Thermo Scientific TurboFlow technology and a unique multiplexing technique to increase the sample throughput of your mass spectrometer (MS). Sae time and simplify method deelopment with integrated software and hardware tools specifically designed to automate deelopment of multidimensional chromatography methods. Transcend systems are optimized to increase productiity, enhance efficiency and boost throughput to your mass spectrometer without compromising data quality or sensitiity. Aria Software Thermo Scientific Aria operating software controls the dual pumping operation, ale switching, cleaning and gradient procedures on the Transcend system. Aria interfaces with all leading mass spectrometer software to optimize the productiity and performance of your instrument. The Windows -based software commands are easy to understand so that methods can be quickly created. Run a single batch or as many as four sample batches simultaneously each with a unique method. The exclusie software allows both TurboFlow methods and LC methods to run on one Transcend system, injection to injection. One screen is all you need to iew the method conditions. Aria operating software automates online operation of the Transcend system. Seamless LC/MS interface Flexible setup and data handling Greater reproducibility of data Simplified training The easy-to-use Aria graphical user interface makes it simple to run a TurboFlow method or set up multiplexing. kda The TurboFlow method remoes high molecular weight material better than traditional methods. A. 1,-fold dilution of matrix B. TurboFlow Method C. Generic SPE D. Protein Precipitation

4 Transcend System Bioanalytical Assays The fast-paced nature of drug discoery a process streamlined to discoer the next blockbuster drug has sparked the need for rapid sample preparation, traditionally the bottleneck in the discoery workflow. Effectie sample preparation is essential to minimize matrix effects and to obtain accurate analytical data so that scientists and researchers can make the right decision. Matrix effect occurs when factors other than target analyte concentration affect electrospray ionization (ESI) response. This harmful effect can compromise or inalidate data. It might een result in selecting drug candidates based on erroneous data. The Transcend system has two critical benefits: fast and effectie online sample preparation that improes confidence of results and multiplexing for increased productiity without compromising data quality. TurboFlow technology enables users to directly inject biological samples into the LC/MS system prior to analysis, a significant adantage in small molecule quantitation during drug research. Better quality data System flexibility to match lab workflow Reduced ion suppression More analysis per day per MS Adanced, multi-dimensional LC Compound X in rat plasma after PPT clean-up Compound X in rat plasma with TurboFlow Technology TurboFlow methods reduce ion suppression s. PPT.

5 Food Safety Accurately monitoring chemical residue leels in food and agricultural products is essential to food supply safety and managing global health risks. The analysis of chemical residues requires techniques sensitie enough to detect and quantify contaminants at or below the maximum residue limit (MRL) in a gien sample matrix. In addition, because of increased food safety regulations and the growing number of samples to be analyzed, the analytical methods must proide high sample throughput. TurboFlow technology proides reliable sample analysis of chemical residues in food. Online sample preparation with the Transcend system and analysis with LC/MS can reduce oerall analytical time compared to traditional offline methods, such as liquid-liquid or solid phase extraction. In addition, this unique process can minimize ion suppression and matrix interference in the LC/MS analysis of food samples. Add to this the multiplexing feature, and you can further increase sample throughput and efficiency in your laboratory without compromising data quality or sensitiity. Cleans difficult matrices honey, milk, wine, shrimp, fish, pig lier prior to LC/MS Reduces sample prep steps less manual labor and time Automates online extraction Chromatogram of 5 fluoro quinolone antibiotic standards at 5 ppb using online clean-up with TurboFlow technology. The system proided a sensitie and reliable analytical method of detecting a full range of antibiotics in honey.

6 Clinical Research and Forensic Toxicology Today s laboratory must be prepared for many challenges among them, to deelop sensitie, specific, reliable methods that are easily reproducible. In order to address this challenge, these same labs are adding LC/MS technology to their workbench. TurboFlow technology increases efficiency in the lab by reducing the amount of time spent on sample preparation especially for complex biological matrices. With TurboFlow methods, endogenous matrix interferences are automatically eliminated from proteins, salts, and phospholipids commonly found in the LC/MS analysis of biological extracts. In fact, biological samples often require little or no handling before injecting them into the Transcend system. The unique multiplexing capability of the Transcend system increases throughput by up to four times. This maximizes the utility of your mass spectrometer and enhances your lab s return on inestment. We employ a complete staff of application chemists and serice personnel to train customers and ensure their labs are up-andrunning within a month. The Transcend system enables LC/MS to fit more effectiely into the routine workflows of clinical research and forensic toxicology laboratories. Increases throughput and flexibility Reduces sample preparation steps Decreases cost per analysis Offers total solutions for clinical research and forensic toxicology, including therapeutic drug monitoring, drugs of abuse, steroids, and other endogenous analytes of interest Manual Handling of 1 Filter Paper Disc Samples Punch 3/16 Disc Punch 3/16 Disc Add IS Solution Add IS / Extraction Solent 1.5e6 System 1 System 2 System 3 System e6 1.5e6 1.5e6 Mix Extract with Ether Remoe Supernatant Mix Intensity, cps 1.e6 1.e6 1.e6 1.e6 Repeat Extraction 5.e5 5.e5 5.e5 5.e5 Combine Extracts Eaporate Ether 1.e5 1.e5 1.e5 1.e5 Reconstitute in Mobile Phase 1 2 Min 1 2 Min 1 2 Min 1 2 Min Transfer Inject Transfer Inject 39 minutes 6 minutes Inject Sys 1 Inject Sys 2 Inject Sys 3 Inject Sys Minutes Inject Sys 1 16 Sample preparation of 1 filter paper disc samples was reduced from 39 minutes with liquid-liquid extraction to 6 minutes with TurboFlow technology. Chromatograms show results of the filter paper extracts run on a 4-channel multiplexing Transcend system with one mass spectrometer. Results 4 times faster than any other single channel LC/MS system.

7 Equine Doping At the highest leel of competitie sports, stakes are high unfortunately so is the temptation to use performance-enhancing drugs. To presere the integrity of sports and ensure fairness, athletes must undergo rigorous qualitatie and quantitatie testing. Accordingly, sports doping and its testing results has gained considerable global attention. Because of this focus, labs must aoid errors typically associated with manual sample preparation, such as SPE and LLE. With TurboFlow technology, methods are often made simple: typically serum is simply diluted, centrifuged, and placed onto the Transcend system for automated, multidimensional LC/MS analysis. The Transcend system can also multiplex sample introduction. This unique feature results in increased throughput up to four times significantly maximizing utilization of the aluable mass spectrometer. When time is of the essence, the benefits of multiplexing are priceless. Not only does the system simplify sample preparation, the Transcend system eliminates the additional time and costs associated with traditional methods. Produces results quickly Proides high confidence leel in data quality Runs multiple methods simultaneously Nandralone Retention Time Window m/z Negatie Control Serum Matrix m/z Water Blank Matrix The Transcend system minimizes ion suppression and matrix effects caused by salt, proteins and phospholipids. Trenbolone Boldenone Nandrolone Testosterone Stanozolol A multiplexed Transcend system addresses all the challenges needed to rapidly analyze low-leel androgenic steroids which are used as performance-enhancing drugs in horse racing.

8 Laboratory Solutions Backed by Worldwide Serice and Support Tap our expertise throughout the life of your instrument. Thermo Scientific Serices extends its support throughout our worldwide network of highly trained and certified engineers who are experts in laboratory technologies and applications. Put our team of experts to work for you in a range of disciplines from system installation, training and technical support, to complete asset management and regulatory compliance consulting. Improe your productiity and lower the cost of instrument ownership through our product support serices. Maximize uptime while eliminating the uncontrollable cost of unplanned maintenance and repairs. When it s time to enhance your system, we also offer certified parts and a range of accessories and consumables suited to your application. To learn more about our products and comprehensie serice offerings, isit us at In addition to these offices, Thermo Fisher Scientific maintains a network of representatie organizations throughout the world. Africa-Other analyze.sa@thermo.com Australia analyze.au@thermo.com Austria analyze.at@thermo.com Belgium analyze.be@thermo.com Canada analyze.ca@thermo.com China analyze.cn@thermo.com Denmark analyze.dk@thermo.com Europe -Other analyze.emea@thermo.com Finland / Norway / Sweden analyze.se@thermo.com France analyze.fr@thermo.com Germany analyze.de@thermo.com India analyze.in@thermo.com Italy analyze.it@thermo.com Japan analyze.jp@thermo.com Latin America analyze.la@thermo.com Middle East analyze.emea@thermo.com Netherlands analyze.nl@thermo.com South Africa analyze.sa@thermo.com Spain analyze.es@thermo.com Switzerland analyze.ch@thermo.com UK analyze.uk@thermo.com USA analyze.us@thermo.com Thermo Scientific Product Representatie turboflow@thermo.com For research use only. Not for use in diagnostic procedures. Thermo Fisher Scientific, San Jose, CA USA is ISO Certified. 29 Thermo Fisher Scientific Inc. All rights resered. Windows is a registered trademark of Microsoft Corporation in the United States and other countries. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Specifications, terms and pricing are subject to change. Not all products are aailable in all countries. Please consult your local sales representatie for details. BR62966_E 4/9S