MP Biomedicals GREAT OFFERS ON. One Call. One Source. A World of. Cell Culture Products.

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1 One Call One Source MP Biomedicals GREAT OFFERS ON Cell Culture Products A World of Cell Culture Products GROWING HEALTHY CELLS MP Biomedicals Fountain Parkway Solon, OH tel: fax: Milenka Arispe, Ph.D. tel: x milenka.arispe@mpbio.com skype ID: milenka.mpbiomedicals

2 Serum free Media MP Biomedicals Media Supplements MP Biomedicals offers a range of cell culture media products designed to support optimal cell growth under defined conditions. The products offer the best performance for your cells and are guaranteed to be of the highest quality and consistency. The media products are available in powder and liquid forms. New Product Release Introductory Offer ChromatoPur Proteins ChromatoPur proteins have been purified by chromatography and ensure highest purity, consistency and activity in cell culture. Unlike with other extraction procedures (e.g. Cohn, Heat Shock) ChromatoPur proteins are intact proteins and highly remain their cell growth stimulating activity. Proteins are purified from New Zealand sourced material under cgmp on an ISO 9001 system, audited by NZFSA MAF. superior performance over Cohn s Albumin Fraction V intact proteins with high activity highest purity and consistency Relative Cell Density Still using Cohn`s Albumin Fraction V? Get better results! Albumin, Bovine Serum, Low Endotoxin Albumin, Bovine Serum, Low IgG Albumin, Bovine Serum,Low Fatty Acid Transferrin, Bovine Serum (holo), iron saturated 25 g 100 g 1 kg 25 g 100 g 1 kg 25 g 100 g 500 g 1 kg 100 mg Transferrin Bovine Serum (apo), iron depleted 100 mg 2

3 MP-Hybridoma Medium, Serum Free Low protein content (11µg/ml) Chemically defined, no animal derived products Supports hybridoma cells, as well as lymphoid and epithelial cells Opti Clone II Hybridoma Cloning Factor Opti-Clone II is a serum-free growth medium supplement which improves the cloning efficiency of murine B cell hybridomas. high efficient growth supplement - up to 25% higher yield on mab increases cells health higher viability of cells protein free - facilitates downstream purification chemically defined no variation in performance means consistent results Hybridoma Growth after fusion 150 avg. no of colonies 120 % colonies producing antibodies RPMI % FBS 7 RPMI % FBS + Growth Supplement used media RPMI % FBS + Opti-Clone II IgG Production [mg/ l ] Hybridoma Cell Culture 250 RPMI + insulin 200 RPMI + insulin + 10% Opti-Clone II culture timeline [h] Optimize Your Monoclonal Yield today! Opti-Clone II growth supplement 10 ml ml MP Hybridoma (without hormones) MP Hybridoma Plus (incl. hormones, hybridoma specific factors) 3

4 TCH & TCM serum replacement for every cell culture Most existing defined serum replacements have been customized for specific cell line. TCH and TCM has been designed to provide an universal serum replacement to suit a wide spectrum of cell types for standard cultures and protein production. no risk of viral or mycoplasma contamination lot-to-lot consistency facilitate down stream protein purification steps TCM serum replacement, standard culture, 50x concentrate 20 ml TCM serum replacement Insulin Free for standard culture, 50x concentrate, 20 ml TCH serum replacement (protein production), 50 x concentrate 20 ml Serum Free Media The use of fetal bovine serum raises concerns such as biological contamination and higher complexity in assays and downstream purification. Also the natural lot-to lot variation of performance makes testing and sourcing cumbersome. less variable and more defined better control and consistent performance better evaluation of cellular functions in assays Get your cells serum free media! Cat# Description Application Size X DMEM/Ham's F-12 (1:1) without L-glutamine Basal media for serum-free culture BioRich 1 with L-Glutamine, without Sodium Bicarbonate DMEM/F12 media for transitioning to serum-free conditions 1x10L X Ham's F-10 with glutamine Growth of CHO cells under serumfree conditions. Also used for growth Ham's F-10 with glutamine of fastidious cell lines without sodium bicarbonate Ham's F-12 with glutamine without sodium bicarbonate 1X Ham's F-12 Kaighn's Modification Growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells Increased nutrients for clonal isolation and growth as well as differentiated rat and chicken cell types and primary human liver cells 1x10L 1x10L 4

5 Mammalian Cell Culture Media and Salt Solutions The cell culture media products are designed to support optimal cell growth under defined conditions. The products offer the best performance for your cells and are guaranteed to be of the highest quality and consistency. All products under go quality control test, are tested for endotoxin and toxicity. Salt solutions are produced without sodium bicarbonate to increase stability DMEM with L-Glutamine, without Sodium Bicarbonate 1x10L DMEM, without L-Glutamine DMEM with 20 mm HEPES, without L-glutamine or Sodium Bicarbonate DMEM without Glutamine, Methionine, Cystine DMEM without L-Glutamine, Inositol RPMI 1640 with L-Glutamine RPMI 1640 without L-Glutamine RPMI 1640, with L-Glutamine, without Sodium Bicarbonate 1x10L RPMI 1640 with 20 mm HEPES, without Sodium Bicarbonate and L-Glutamine Easy and convenient 100 tabs Phosphate Buffered Saline Tablets 200 tabs without calcium and magnesium, 1 Tablet = 100 ml 500 tabs X PBS without calcium, magnesium X PBS with magnesium chloride PBS, powder, with calcium, magnesium 1x10L PBS, powder, without calcium, magnesium 10x1L X PBS without calcium, magnesium X HBS with calcium, magnesium and phenol red X HBS without calcium, magnesium X HBS without calcium, magnesium, phenol red X HBS without sodium bicarbonate HBS powder, without sodium bicarbonate 1x10L 5

6 Media Supplements In many cell culture the addition of supplemental nutrients and reagents is required. Additives such as antibiotics and buffers are frequently used to prevent bacterial contamination, control ph and monitor culture conditions. Amino acids and vitamins are useful for enriched media. MP Biomedicals can also provide more media supplements (see our web site for complete listing). Vitamin, Amino Acids and Trace Solution BME Vitamins (100X Solution) MEM 100X Non-Essential Amino Acids 100 ml MEM 50X Amino Acids without L-glutamine 100 ml Trace Element Solution 1, 1000X Solution, Sterile Filtered (CuSO 4, ZnSO 4, Na-Selenite, Ferric Citrate) 100 ml Antibiotic Solutions Antibiotic solutions from MP Biomedicals are proven effective at keeping mammalian cell cultures free of bacterial or fungal contaminations. Mammalian cells are selected while bacteria depending on their resistance die within 7 days Penicillin-Streptomycin Solution 5000 U Pen./ 5 mg Strept./ml 100 ml Penicillin-Streptomycin Solution 10,000 U Pen. /10 mg Strept./ ml 100 ml Penicillin- Streptomycin- Amphotericin B Solution 100 ml ml Amphotericin B Solution 250 ug/ml ml Gentamycin Solution 10 mg/ml 10 ml Gentamycin Solution 50 mg/ml 10 ml Kanamycin Solution 5 mg/ml 50 ml 6

7 Accutase for gentle cell dissociation Accutase is a novel trypsin replacement from MP Biomedicals which is more gentle than trypsin. Accutase exhibits both protease and collagenolytic activities and does not include bacterial or mammalian components. MP Biomedicals trypsin is proven effective and available in two standard formulations Accutase 100 ml Trypsin-EDTA 0.05% Trypsin, 0.02% EDTA (w/v) 100 ml Trypsin-EDTA, 0.25% Trypsin, 1 mm EDTA, in HBSS 100 ml Cell Quantification Solutions Trypan Blue Solution, 0.4% (w/v) in PBS 100 ml Neutral Red Solution, 0.33% 100 ml Preserve your serum free cell line - Cellvation Cellvation is a cryopreservation medium which contains neither DMSO nor Serum and is ideally suited for serum-free cultures. optimal recovery and faster cell attachment all types of serum-dependent cell lines ready to use solution Still using DMSO or serum containing cryopreservation media? Buy a pack of Cellvation today! Cellvation Serum Free preservation 20 ml 7

8 SERUM MP Biomedicals when performance counts Quality of serum and lot-to-lot consistency is essential for successful cell culture. Origin, Blood collection and processing methods higher influence the final quality. MP Biomedicals take special care in the production of sera and guarantee a product of superior performance. Human Serum, Type AB Human serum provides an environment closely to physiological conditions for sensitive human cells lines. Moreover Human AB+ Serum lacks antibodies against the A and B blood type antigens that could react with the cultured cells. The whole blood is allowed to spontaneously clot before the serum component is separated. These individual units of serum are then pooled, sterile filtered, bottled and cell culture tested for mycoplasma, endotoxin, and sterility. supporting growth for lymphocytes and macrophages enhanced cell proliferation mycoplasma, HIV and HBsAg free CELLect Gold FBS Fetal Bovine Serum Each lot is supplied with origin certificate to ensure traceability and each batch is rigorously controlled for the absence of aerobic and anaerobic bacteria, fungi, yeast and Mycoplasma. Biological performance is assessed using cell culture medium supplemented with the serum being tested at a final concentration of 10%. superior quality high performance high consistency CeLLect Gold is low endotoxin level and virus free Human serum Type AB 100 ml CELLect Gold Fetal Bovine Serum 8

9 Keep your Lab Mycoplasma free! ImmuMark Mycotest Detection Kit ImmuMark Mycotest Kit uses a monoclonal antibody for immunofluorescence staining specific against a variety of Mycoplasma species. Mycoplasma appear as a bright green particulate fluorescence against a red background. rapid test reliable results in less than 1h broad range of species detected and ready to go kit very sensitive early detection of contaminants Indirect immunofluorescent staining of M. bovis infected tumoral Ag-8 cell line (Magnification x 400) Mycoplasma Stain Kit The Mycoplasma Stain Kit uses the Hoechst Dye and detect the contaminants in less than 2 hours. This test is rapid and highly sensitive. The kit contains all reagents needed including fixed control slides. Mycoplasma Removal Agent MRA Mycoplasma Removal Agent (MRA) from MP Biomedicals offers a highly effective solution for the elimination of mycoplasma contamination in cell cultures. MRA eliminates mycoplasma infection by inhibiting mycoplasma DNA gyrase. MRA is supplied as ready to use solution and is stable at room temperature. MRA has been regulary used by the European Collection of Animal Cell Cultures for the elimination of mycoplasma. highly effective very low toxicity the gold standard for cell culture collections Keep your lab Mycoplasma free and buy one of the rapid detection and elimination products ImmuMark Mycotest Kit 50 test Mycoplasma Stain Kit 100 test Fixed Control Slide Kit 5 slides Mycoplasma Removal Agent (MRA) Solution 5 ml 9

10 CELL SEPARATION MEDIA Isolation of lymphocytes from peripheral blood. LSM Lymphocyte Separation Medium MP Biomedicals LSM is a proprietary formulation which allows one step separation of lymphocytes from peripheral blood. After centrifugation the pellet consists mostly of erythrocytes and granulocytes which have migrated through the gradient. Due to their density, lymphocytes and other mononuclear cells (platelets and monocytes) are found at the plasma-lsm interface. highest Lyphocyte yields easy to use one step centrifugation ready-to-use formulation Mono- Poly Resolving Medium There is a need for a rapid, simple and reliable method of isolating lymphocytes from venous blood and polymorphonuclear leukocytes. Mono-Poly Resolving Medium from MP Biomedicals is addressing that need for the efficient separation of blood cells. Mono-Poly Resolving Medium is a solution composed of a polysaccharide (Ficoll 400) and a radiopaque contrast medium (Hypaque). The resolving medium enables the resolution of both mononuclear and polymorphonuclear leucocytes into two distinct bands in one step and 99% purity. separation of mononuclear and polymorphonuclear leukocytes in two distinct bands. > 95% cell viability rapid, easy and reliable one step procedure no sample dilution requirement or lysis of red blood cell. Ease your Lymphocyte Separation! LSM Lymphocyte Separation Medium 5x100 ml Mono-Poly Resolving Medium 100 ml LSM free sample available! 10

11 PRIMARY CULTURE & LEUCOCYTES MEDIA 1X McCoy 5A Medium (Park and Terasaki Modification) without L-glutamine This medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP s medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. Medium 199 with Earl s Salts (E199) Medium 199 can be used with a broad rqnge of species including the culturing of non-transformed cell types. It may be used for vaccine production and the in vitro cultivation of rat lens tissues and primary mouse pancreatic epithelial explants. It is available in either Earle s or Hanks salts X McCoy 5A Medium (Park and Terasaki Modification) without L-glutamine 100 ml X Medium 199 with Earl's Salts (E199), with 2.2 g/l sodium bicarbonate without L-glutamine X Medium 199 with Earl's salts (E199), with L glutamine, without sodium bicarbonate 10 x 1L X Medium 199 with Earl's Salts (E199), with 20 mm HEPES, without L glutamine, sodium bicarbonate 100 ml X Medium with Hank s salts (H199) with L glutamine, without sodium bicarbonate 10 x 1L 11

12 Build welcoming homes for your cells Multitest Slide High quality glass slides with a unique blue PTFE coating convenient for cell culture, slide agglutination tests and histology. Resistant to common organic solvents and fixatives Sterilizable 1 hour at 160 Cells only attach to uncoated glass Size: 2.5 cm x 7.5 cm x 1 mm Cat# # of well Well Diameter Wells Array Numbered Frosted E 15 4 mm 3 x 5 No Yes E 12 8 mm 2 x 6 Yes No E 10 8 mm 2 x 5 Yes No E 8* 6 mm 2 x 4 Yes No *The 8-well slides feature the same spacing as standard microplates MP Biomedicals GREAT OFFERS ON Cell Culture Products GROWING HEALTHY CELLS MP Biomedicals Fountain Parkway Solon, OH tel: fax: Milenka Arispe, Ph.D. tel: x milenka.arispe@mpbio.com skype ID: milenka.mpbiomedicals