Biogas Production Microbial Teamwork and Enzymology

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2 Biogas Production Microbial Teamwork and Enzymology Live H. Hagen KBM, Norwegian University of Life Sciences (NMBU), Ås, Norway Contact: CenBio Final Conference Ås Campus March 2017

we explore the microbial world What we found - and

3 Overview Background on biogas production with focus on the microbial teamwork behind Project objectives How we explore the microbial world What we found - and what remains unknown Current status and Future perspective

4 Biogas production Organic waste management Microbial digestion under anaerobic condition Production of biogas (CH 4, CO 2 ) Electricity Heath Biofuel Norwegian University of Life Sciences 4

5 Biogas production Organic waste management Microbial digestion under anaerobic condition Production of biogas (CH 4, CO 2 ) Electricity Heath Biofuel Norwegian University of Life Sciences 5

Enzymology During anaerobic digestion, microbes

different degradation events Enzymology is the

and function, as well as their relation to each

dependent Reconstruction of metabolic pathways

6 Enzymology During anaerobic digestion, microbes produce different enzymes that catalyzes different degradation events Enzymology is the study of enzymes, their kinetics, structure, and function, as well as their relation to each other Hydrolysis substrate-composition dependent Reconstruction of metabolic pathways Tittel på presentasjon Norwegian University of Life Sciences 6

biogas production Monitor and control the

7 Goals: Identify key organisms and metabolic pathways in biogas production Enhanced understanding of the process More stable biogas reactors Improved biogas production Monitor and control the microbial process Norwegian University of Life Sciences 7

Compositions and Interactions Metagenomics Closer

8 Methods Traditional Cultivation High-Throughput Sequencing In depth studies of specific organisms Compositions and Interactions Metagenomics Closer to reality Norwegian University of Life Sciences 8

9 Metagenomics A Metagenome is all the genetic material present in an environmental sample, consisting of the genomes of many individual organisms Metagenomics is the direct genetic analysis of metagenomes Norwegian University of Life Sciences 9

10 Direct Sequencing of the Metagenome Norwegian University of Life Sciences 10

Experiments with Laboratory Biogas Reactors Identified microorganisms related to unstable performance Tested the tolerance for storage Biogas lab facility at

11 Experiments with Laboratory Biogas Reactors Identified microorganisms related to unstable performance Tested the tolerance for storage Biogas lab facility at Vollebekk, Ås Studied the effect of recycling the effluent to dilute food waste Observed an improved process when adding cow manure Norwegian University of Life Sciences 11

12 Plant Scale Thermophilic Reactor FREVAR FK (Fredrikstad): Receives food waste and industrial waste Produce biofuel for ~100 busses Stable for decades at high temperature In-depth characterization Norwegian University of Life Sciences 12

13 16S rrna gene Metagenomics Metaproteomics Who is there? What s their physiological potential? What are they doing? Who is doing what? Norwegian University of Life Sciences 13

14 Having a Genome Sequence, We Can Understand a Microbe s Metabolism Hagen et al., Appl. Environ. Microbiol. January 2017 Norwegian University of Life Sciences 15

15 Plant scale Food Waste Reactor Oslo EGE: Receives food waste from the city of Oslo Produces biofuel and bio-fertilizer First microbial characterization of this plant, from start to stable Norwegian University of Life Sciences 16

start monitoring the process: Study trends to foresee coming events Detect the process

16 Achievements and Current Status From lab-scale studies to plant scale application Methodology: Functional analysis provides a more complete picture On a stage where we can start monitoring the process: Study trends to foresee coming events Detect the process problems at an earlier stage Control the microbial community Norwegian University of Life Sciences 17

17 Future Perspective Still a lot unexplored! Down to basic: Design less complex lignocellulose-degrading communities Can we discover novel enzymes and novel metabolic functions? Learn from the Nature Apply in industry Biogas production in a biorefinery context Anaerobic digestion of waste streams Production of higher-value compounds Norwegian University of Life Sciences 18

Arntzen Chris Gaby Mirzaman Zamanzadeh Thank you for your

18 Acknowledgements NMBU team PEP/BTB group: Svein J. Horn Phil B. Pope Vincent G.H. Eijsink Jeremy Frank Magnus Ø. Arntzen Chris Gaby Mirzaman Zamanzadeh Thank you for your attention! Contact: Norwegian University of Life Sciences 19

19 Thank you for your attention! Contact: 20

Anaerobic Digester Microbial Community, Metadata, and Outcomes. Alison Ling, Barr Engineering in collaboration with Microbe Detectives

Anaerobic Digester Microbial Community, Metadata, and Outcomes. Alison Ling, Barr Engineering in collaboration with Microbe Detectives Anaerobic Microbial Community, Metadata, and Outcomes Alison Ling, Barr Engineering in collaboration with Microbe Detectives Can we give operators more tools to troubleshoot digesters, especially with