ab Anti-ENA Screen Human ELISA Kit
|
|
- Duane Lane
- 6 years ago
- Views:
Transcription
1 ab Anti-ENA Screen Human ELISA Kit Instructions for Use For the semi-quantitative measurement of IgG class antibodies against extractable nuclear antigens (ENA) in Human serum and plasma. This product is for research use only and is not intended for diagnostic use. Version 1 Last Updated 8 November 2013
2 Table of Contents INTRODUCTION 1. BACKGROUND 2. ASSAY SUMMARY 2 4 GENERAL INFORMATION 3. PRECAUTIONS 4. STORAGE AND STABILITY 5. MATERIALS SUPPLIED 6. MATERIALS REQUIRED, NOT SUPPLIED 7. LIMITATIONS 8. TECHNICAL HINTS ASSAY PREPARATION 9. REAGENT PREPARATION 10. SAMPLE COLLECTION AND STORAGE 11. SAMPLE PREPARATION 12. PLATE PREPARATION ASSAY PROCEDURE 13. ASSAY PROCEDURE 10 DATA ENALYSIS 14. CALCULATIONS 15. TYPICAL SAMPLE VALUES 16. ASSAY ANALYTICAL SPECS RESOURCES 17. TROUBLESHOOTING 18. NOTES
3 PRODUCT INFORMATION 1. BACKGROUND Abcam s anti-ena Screen Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate semi-quantitative measurement of IgG class antibodies against extractable nuclear antigens: Sm (Smith), RNP/Sm, Scl-70, SS-A (Ro) (52kDa and 60kDa), SS-B (La), Jo-1, in Human serum or plasma. A 96-well plate has been precoated with ENA antigens to bind cognate antibodies. Controls, standards or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added to the wells, which binds to the immobilized ENA-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of ENA IgG sample captured in plate. Autoimmune diseases are characterized by an abnormal functioning of the immune system which mistakenly attacks healthy body tissue. It results in a change of the mechanisms of cell recognition; the organism no longer distinguishes the "self" from "not-self", ie the elements belonging to it and those that do not. This leads to production of antibodies that can affect individual organs or trigger systemic diseases, damaging entire functions of the individual. The processes that lead to serious autoimmune disease are still unknown in many ways. Rheumatic autoimmune diseases are often associated with the presence of many autoantibodies directed against nuclear and cytoplasmic antigens. These antibodies are commonly called antinuclear antibodies (ANA) and their presence is important for the detection of different connective tissue diseases. Although the detection of ANA is very significant for the diagnosis of autoimmune disorders, it is not very specific. Therefore it is advisable to detect for the presence of antibodies against extractable nuclear antigens (ENA). 2
4 PRODUCT INFORMATION The six most significant and investigated anti-ena autoantibodies are those against Sm, RNP/Sm, SS-A, SS-B, SCL-70 and Jo1. The ENA Screen can provide important diagnostic information on patients with suspected connective tissue diseases such as systemic lupus erythematosus (SLE), polymyositis, Sjogren syndrome, scleroderma and mixed connective tissue disease (MCTD). Every disease does not present a single parameter, but results in the simultaneous presence of multiple parameters, although there are autoantibodies that characterize pathology better than others. For example, in Sjogren syndrome there are mainly antibodies against SS-A and SS-B, while in MCTD the most relevant antibodies are those against Sm and RNP/Sm. 3
5 PRODUCT INFORMATION 2. ASSAY SUMMARY Prepare all reagents, samples and controls as instructed. Add samples and controls to wells used. Incubate at 37 C. Wash each well and add prepared labeled HRP-Conjugate. Incubate at room temperature. After washing, add TMB substrate solution to each well. Incubate at room temperature. Add Stop Solution to each well. Read immediately. 4
6 GENERAL INFORMATION 3. PRECAUTIONS Please read these instructions carefully prior to beginning the assay. All kit components have been formulated and quality control tested to function successfully as a kit. Modifications to the kit components or procedures may result in loss of performance. 4. STORAGE AND STABILITY Store kit at immediately upon receipt. Refer to list of materials supplied for storage conditions of individual components. Observe the storage conditions for individual prepared components in section 9. Reagent Preparation. 5. MATERIALS SUPPLIED ENA Coated Microplate (12 x 8 wells) 96 Wells Storage Condition (Before Preparation) IgG Sample Diluent 100 ml Stop Solution 15 ml 10X Washing Solution 50 ml ENA anti-igg HRP Conjugate 15 ml TMB Substrate Solution 15 ml ENA Standard 1.2 ml ENA Positive Control 1.2 ml ENA Negative Control Item Amount 1.2 ml Strip Holder 1 unit Cover Foil 1 unit 5
7 GENERAL INFORMATION 6. MATERIALS REQUIRED, NOT SUPPLIED These materials are not included in the kit, but will be required to successfully utilize this assay: Microplate reader capable of measuring absorbance at 450 nm or 620 nm Incubator at 37 C Multi and single channel pipettes to deliver volumes between 10 and 1,000 µl Optional: Automatic plate washer for rinsing wells Vortex tube mixer Deionised or (freshly) distilled water Disposable tubes Timer 7. LIMITATIONS ELISA kit intended for research use only. Not for use in diagnostic procedures All components of Human origin used for the production of these reagents have been tested for anti-hiv antibodies, anti-hcv antibodies and HBsAg and have been found to be non-reactive. Nevertheless, all materials should still be regarded and handled as potentially infectious Use only clean pipette tips, dispensers, and lab ware. Do not interchange screw caps of reagent vials to avoid crosscontamination Close reagent vials tightly immediately after use to avoid evaporation and microbial contamination After first opening and subsequent storage check conjugate and control vials for microbial contamination prior to further use 6
8 GENERAL INFORMATION To avoid cross-contamination and falsely elevated results pipette patient samples and dispense conjugate without splashing accurately to the bottom of wells 8. TECHNICAL HINTS Avoid foaming components Avoid cross contamination of samples or reagents by changing tips between sample, standard and reagent additions. Ensure plates are properly sealed or covered during incubation steps Complete removal of all solutions and buffers during wash steps is necessary for accurate measurement readings This kit is sold based on number of tests. A test simply refers to a single assay well. The number of wells that contain sample, control or standard will vary by product. Review the protocol completely to confirm this kit meets your requirements. Please contact our Technical Support staff with any questions or bubbles when mixing or reconstituting 7
9 ASSAY PREPARATION 9. REAGENT PREPARATION Equilibrate all reagents, samples and controls to room temperature (18-25 C) prior to use X Washing Solution Prepare 1X Washing Solution by diluting 10X Washing Solution with deionized water. To make 500 ml 1X Washing Solution combine 50 ml 20X Washing Solution with 450 ml deionized water. Mix thoroughly and gently. All other solutions are supplied ready to use 10. SAMPLE COLLECTION AND STORAGE Use Human serum or plasma (citrate) samples with this assay. If the assay is performed within 5 days of sample collection, the specimen should be kept at ; otherwise they should be aliquoted and stored deep-frozen (-20 to -80 C). If samples are stored frozen, mix thawed samples well before testing. Avoid repeated freezing and thawing. Heat inactivation of samples is not recommended 11. SAMPLE PREPARATION Before assaying, all samples should be diluted 1:100 with IgG Sample Diluent. Add 10 µl sample to 990 µl IgG Sample Diluent to obtain a 1:100 dilution. Mix gently and thoroughly. 8
10 ASSAY PREPARATION 12. PLATE PREPARATION The 96 well plate strips included with this kit are supplied ready to use. It is not necessary to rinse the plate prior to adding reagents Unused well strips should be returned to the plate packet and stored at 4 C For each assay performed, a minimum of 1 well must be used as a blank, omitting sample and conjugate from well addition For statistical reasons, we recommend each standard and sample should be assayed with a minimum of two replicates (duplicates) 9
11 ASSAY PROCEDURE 13. ASSAY PROCEDURE Equilibrate all materials and prepared reagents to room temperature prior to use. Please read the test protocol carefully before performing the assay. Reliability of results depends on strict adherence to the test protocol as described. All controls (ENA Positive Control, ENA Negative Control) must be included with each assay performed to determine test results If performing the test on ELISA automatic systems we recommend increasing the washing steps from three to five and the volume of washing solution from 300 µl to 350 µl to avoid washing effects. Assay all standards, controls and samples in duplicate Prepare all reagents, working standards, and samples as directed in the previous sections Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, reseal and return to 4 C storage Add 100 µl of each standard, sample or control into appropriate wells. Leave one well for substrate blank Cover wells with the foil supplied in the kit and incubate for 30 minutes at room temperature Remove the foil, aspirate the contents of the wells and wash each well three times with 300 µl of 1X Washing Solution. Avoid spill over into neighboring wells. The soak time between each wash cycle should be >5 sec. After the last wash, remove the remaining 1X Washing Solution by aspiration or decanting. Invert the plate and blot it against clean paper towels to remove excess liquid. Note: Complete removal of liquid at each step is essential for good assay performance. 10
12 ASSAY PROCEDURE Add 100 µl ENA anti-igg HRP Conjugate into all wells except for the blank well. Cover with foil Incubate for 30 minutes at room temperature. Do not expose to direct sunlight Repeat step Add 100 µl TMB Substrate Solution into all wells Incubate for exactly 15 minutes at room temperature in the dark Add 100 µl Stop Solution into all wells in the same order and at the same rate as for the TMB Substrate Solution. Note: Any blue color developed during the incubation turns into yellow Measure the absorbance of the specimen at 450 nm within 30 minutes of addition of the Stop Solution. Dual wavelength reading using 620 nm as reference wavelength is recommended. 11
13 DATA ANALYSIS 14. CALCULATIONS In order for an assay to be considered valid, the following criteria must be met: Substrate blank: Negative control: Absorbance value < and < cut-off Cut-off control: Positive control: Absorbance value > cut-off Absorbance value < Absorbance value If these criteria are not met, the test is not valid and must be repeated. Calculation of Results Calculate the mean background subtracted absorbances for each sample and compare to mean Cut-off control value. The Cut-off control value is the mean absorbance value of the Cut-off control wells. Example: Absorbance value Cut-off control Well 1 = Absorbance value Cut-off control Well 2 = Mean Cut Off value: ( )/2 = Interpretation of Results Samples are considered to give a positive signal if the absorbance value is greater than 10% over the cut-off value. Samples with an absorbance value of less than 10% above or below the Cut-off control value should be considered as inconclusive (grey zone) i.e. neither positive or negative. It is recommended to repeat the assay using fresh samples. If results of the second test are again less than 10% above or below the Cut-off control value the sample has to be considered negative. Samples are considered negative if the absorbance value is lower than 10% below the cut-off. 12
14 DATA ANALYSIS Results in Standard Units Patient (mean) absorbance value x 10 = Standard Units Cut-off Example: x 10 = 47 Standard Units 0.38 Cut-off: 10 Standard Units Grey zone: 9-11 Standard Units Negative: <9 Standard Units Positive: >11 Standard Units 13
15 DATA ANALYSIS 15. TYPICAL SAMPLE VALUES PRECISION n= %CV Intra-Assay Inter-Assay REFERENCE VALUES In a normal range study with serum samples from healthy blood donors the following ranges have been established with the Anti-ENA Screen ELISA Kit: anti-ena U/mL Normal < 25 Elevated > ASSAY ANALYTICAL SPECS SPECIFICITY The specificity is > 92.3 % and is defined as the probability of the assay scoring negative in the absence of the specific analyte. SENSITIVITY The sensitivity is 94.7% and is defined as the probability of the assay scoring positive in the presence of the specific analyte. The concentration of the analyte that can be distinguished from the zero standard is 3.68 U/mL. 14
16 RESOURCES 17. TROUBLESHOOTING Problem Cause Solution Incubation time to short Try overnight incubation at 4 C Precipitate can form in wells upon substrate addition when concentration of target is too high Increase dilution factor of sample Using incompatible sample type (e.g. serum vs. cell extract) Detection may be reduced or absent in untested sample types Sample prepared incorrectly Ensure proper sample preparation/dilution Bubbles in wells Ensure no bubbles present prior to reading plate All wells not washed equally/thoroughly Check that all ports of plate washer are unobstructed/wash wells as recommended Incomplete reagent mixing Ensure all reagents/master mixes are mixed thoroughly Inconsistent pipetting Use calibrated pipettes & ensure accurate pipetting Inconsistent sample preparation or storage Ensure consistent sample preparation and optimal sample storage conditions (e.g. minimize freeze/thaws cycles) 15 Low signal Large CV
17 RESOURCES Problem Cause Solution Wells are insufficiently washed Wash wells as per protocol recommendations Contaminated wash buffer Make fresh wash buffer Waiting too long to read plate after adding stop solution Read plate immediately after adding stop solution Improper storage of ELISA kit Store all reagents as recommended. Please note all reagents may not have identical storage requirements. Using incompatible sample type (e.g. Serum vs. cell extract) Detection may be reduced or absent in untested sample types 16 High background Low sensitivity
18 RESOURCES 18. NOTES 17
19 RESOURCES 18
20 UK, EU and ROW Tel: +44-(0) Austria Tel: France Tel: Germany Tel: Spain Tel: Switzerland Tel (Deutsch): Tel (Français): US and Latin America Tel: ABCAM (22226) Canada Tel: China and Asia Pacific Tel: (中國聯通) Japan Tel: +81-(0) Copyright 2013 Abcam, All Rights Reserved. The Abcam logo is a registered trademark. All information / detail is correct at time of going to print. RESOURCES 19
ab Anti-Sm IgG ELISA Kit
ab178627 Anti-Sm IgG ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against Sm in Human serum and plasma. This product is for research use only and is not intended
More informationab Anti-RNP70 IgG ELISA Kit
ab178625 Anti-RNP70 IgG ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against RNP70 in Human serum and plasma. This product is for research use only and is not
More informationab Anti-MPO (p-anca) ELISA Kit
ab178630 Anti-MPO (p-anca) ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against myeloperoxidase (MPO) in Human serum and plasma. This product is for research
More informationab Anti-Malaria ELISA Kit
ab178649 Anti-Malaria ELISA Kit Instructions for Use For the qualitative measurement of IgG and IgM class antibodies against Malaria in Human serum and plasma (citrate). This product is for research use
More informationab Anti-PR 3 (c-anca) IgG ELISA Kit
ab178623 Anti-PR 3 (c-anca) IgG ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against PR 3 (c-anca) in Human serum and plasma. This product is for research use
More informationab Anti-Cardiolipin IgG ELISA Kit
ab178613 Anti-Cardiolipin IgG ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against Cardiolipin in Human serum and plasma. This product is for research use only
More informationab Anti-Deamidated Gliadin Peptide (DGP) IgA ELISA Kit
ab178616 Anti-Deamidated Gliadin Peptide (DGP) IgA ELISA Kit Instructions for Use For the quantitative measurement of IgA class antibodies against Deamidated Gliadin Peptide (DGP) in Human serum and plasma.
More informationab alpha Fetoprotein (AFP) Human ELISA Kit
ab108631 alpha Fetoprotein (AFP) Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of alpha Fetoprotein in Human Serum. This product is for research use only
More informationab MBL (Mannose-Binding Lectin) Human ELISA Kit
ab193709 MBL (Mannose-Binding Lectin) Human ELISA Kit Instructions for Use For the quantitative measurement of Human MBL (Mannose-Binding Lectin) in serum, plasma and cell culture supernatants. This product
More informationab IL10 (Interleukin-10) Canine ELISA Kit
ab193685 IL10 (Interleukin-10) Canine ELISA Kit Instructions for Use For the quantitative measurement canine IL10 in serum, plasma and cell culture supernatants. This product is for research use only and
More informationab FAP Human ELISA Kit
ab193701 FAP Human ELISA Kit Instructions for Use For the quantitative measurement of human Fibroblast Activation Protein, alpha (FAP) in serum, plasma, and cell culture supernatants. This product is for
More informationab TIMP2 Human ELISA Kit
ab100653 TIMP2 Human ELISA Kit Instructions for Use For the quantitative measurement of Human TIMP2 in serum, plasma and cell culture supernatants. This product is for research use only and is not intended
More informationab Anti-Herpes simplex virus Type 2 (HSV2) IgG Human ELISA Kit
ab108739 Anti-Herpes simplex virus Type 2 (HSV2) IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Herpes simplex virus Type 2 (HSV2) in Human serum
More informationab CD163 (M130) Human ELISA Kit
ab155428 CD163 (M130) Human ELISA Kit Instructions for Use For the quantitative measurement of Human CD163 in serum, plasma and cell culture supernatants. This product is for research use only and is not
More informationab anti-phospholipid screen IgG and IgM ELISA Kit
ab178622 anti-phospholipid screen IgG and IgM ELISA Kit Instructions for Use For the quantitative measurement of IgG and IgM class antibodies against Phospholipids in Human serum and plasma. This product
More informationab Anti-Respiratory syncytial virus (RSV) IgG Human ELISA Kit
ab108765 Anti-Respiratory syncytial virus (RSV) IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Respiratory syncytial virus (RSV) in Human serum
More informationab Chorionic Gonadotropin beta Human ELISA Kit
ab108638 Chorionic Gonadotropin beta Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of Chorionic Gonadotropin beta in Human Serum. This product is for research
More informationab Histone H2B Acetyl (K5) ELISA Kit
ab138916 Histone H2B Acetyl (K5) ELISA Kit Instructions for Use For the quantitative measurement of acetylated lysine (K5) on Histone H2B in cell lysates. This product is for research use only and is not
More informationab Ferritin Human ELISA Kit
ab108698 Ferritin Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of Ferritin in serum and plasma. This product is for research use only and is not intended
More informationab Ferritin Human ELISA Kit
ab108698 Ferritin Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of Ferritin in serum and plasma. This product is for research use only and is not intended
More informationab Anti-Chagas IgG Human ELISA Kit
ab178637 Anti-Chagas IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Chagas in Human serum and plasma (citrate). This product is for research use
More informationab Anti-Schistosoma mansoni IgG Human ELISA Kit
ab108769 Anti-Schistosoma mansoni IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma (citrate). This
More informationab Anti-Schistosoma mansoni IgG Human ELISA Kit
ab108769 Anti-Schistosoma mansoni IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Schistosoma mansoni in Human serum and plasma (citrate). This
More informationab Anti-Helicobacter pylori IgG Human ELISA Kit
ab108736 Anti-Helicobacter pylori IgG Human ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma (citrate). This
More informationab Leptin Rat ELISA Kit
ab100773 Leptin Rat ELISA Kit Instructions for Use For the quantitative measurement of rat Leptin in serum, plasma and cell culture supernatants. This product is for research use only and is not intended
More informationab Anti-Candida albicans IgA Human ELISA Kit
ab108714 Anti-Candida albicans IgA Human ELISA Kit Instructions for Use For the qualitative measurement of IgA class antibodies against Candida albicans in Human serum and plasma (citrate). This product
More informationab Anti-Candida albicans IgA Human ELISA Kit
ab108714 Anti-Candida albicans IgA Human ELISA Kit Instructions for Use For the qualitative measurement of IgA class antibodies against Candida albicans in Human serum and plasma (citrate). This product
More informationab46107 IFN gamma Rat ELISA Kit
ab46107 IFN gamma Rat ELISA Kit Instructions for Use For the quantitative measurement of rat IFN gamma in cell culture supernatants, buffered solutions and plasma. This product is for research use only
More informationab Anti-Parvovirus B 19 IgM ELISA Kit
ab178651 Anti-Parvovirus B 19 IgM ELISA Kit Instructions for Use For the qualitative measurement of IgM class antibodies against Parvovirus B 19 in Human serum and plasma (citrate). This product is for
More informationab IgY Chicken ELISA Kit
ab157693 IgY Chicken ELISA Kit Instructions for Use For the quantitative measurement of IgY in Chicken serum and plasma samples. This product is for research use only and is not intended for diagnostic
More informationab IL-1 alpha (Interleukin-1 alpha) Human ELISA Kit
ab100560 IL-1 alpha (Interleukin-1 alpha) Human ELISA Kit Instructions for Use For the quantitative measurement of Human IL-1 alpha in serum, plasma, and cell culture supernatants. This product is for
More informationab Anti-Coxiella burnetii (Q-Fever) Phase 2 IgG ELISA Kit
ab178639 Anti-Coxiella burnetii (Q-Fever) Phase 2 IgG ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Coxiella burnetii in Human serum. This product is for
More informationab Prolactin Human ELISA Kit
ab108655 Prolactin Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of Prolactin in Human Serum. This product is for research use only and is not intended
More informationab Immunoglobulin E (IgE) Human ELISA Kit
ab178659 Immunoglobulin E (IgE) Human ELISA Kit Instructions for Use An immunoenzymatic assay for the quantitative measurement of Immunoglobulin E (IgE) in Human serum and plasma. This product is for research
More informationab Anti-Toxoplasma gondii IgG Human ELISA Kit
ab108776 Anti-Toxoplasma gondii IgG Human ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against Toxoplasma gondii in Human serum and plasma (citrate). This product
More informationab TGF beta 1 Human ELISA Kit
ab108912 TGF beta 1 Human ELISA Kit Instructions for Use For the quantitative measurement of Human TGF beta 1 in cell culture supernatants. This product is for research use only and is not intended for
More informationab99995 C Reactive Protein (CRP) Human ELISA Kit
ab99995 C Reactive Protein (CRP) Human ELISA Kit Instructions for Use For the quantitative measurement of Human CRP in serum, plasma and cell culture supernatants. This product is for research use only
More informationab99978 BDNF Human ELISA Kit
ab99978 BDNF Human ELISA Kit Instructions for Use For the quantitative measurement of Human BDNF in serum, plasma, and cell culture supernatants. This product is for research use only and is not intended
More informationab45910 CD31 (PECAM1) Human ELISA Kit
ab45910 CD31 (PECAM1) Human ELISA Kit Instructions for Use For the quantitative measurement of CD31 (PECAM1) in Human serum, plasma, buffered solutions and cell culture media. This product is for research
More informationab Free Testosterone ELISA Kit
ab178663 Free Testosterone ELISA Kit Instructions for Use A competitive immunoenzymatic assay for the quantitative measurement of Free Testosterone in serum and plasma (citrate). This product is for research
More informationab Anti-Mycoplasma pneumoniae IgG Human ELISA Kit
ab108755 Anti-Mycoplasma pneumoniae IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Mycoplasma pneumoniae in Human serum and plasma (citrate). This
More informationab IgG Guinea pig ELISA Kit
ab190525 IgG Guinea pig ELISA Kit Instructions for Use For the quantitative measurement of IgG in Guinea pig serum samples. This product is for research use only and is not intended for diagnostic use.
More informationab Testosterone ELISA Kit
ab108666 Testosterone ELISA Kit Instructions for Use A competitive immunoenzymatic assay for the quantitative measurement of Testosterone in serum, plasma and urine This product is for research use only
More informationab Elafin/Skalp Human ELISA Kit
ab100658 Elafin/Skalp Human ELISA Kit Instructions for Use For the quantitative measurement of Human Elafin in serum, plasma and cell culture supernatants. This product is for research use only and is
More informationab Serum Amyloid P (APCS) Rat ELISA Kit
ab157741 Serum Amyloid P (APCS) Rat ELISA Kit Instructions for Use For the quantitative measurement of Serum Amyloid P (APCS) in Rat serum and plasma samples. This product is for research use only and
More informationCancer Antigen CA125 Human ELISA Kit
ab108653 Cancer Antigen CA125 Human ELISA Kit Instructions for Use For the quantitative measurement of Human Cancer Antigen CA125 concentrations in serum This product is for research use only and is not
More informationab DHEA sulfate (DHEA-S) ELISA Kit
ab108669 DHEA sulfate (DHEA-S) ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against DHEA sulfate (DHEA-S) in Human serum and plasma (citrate). This product is
More informationab Transferrin Rat ELISA Kit
ab137992 Transferrin Rat ELISA Kit Instructions for Use For the quantitative measurement of Rat Transferrin in urine and cell culture supernatants. This product is for research use only and is not intended
More informationab Testosterone ELISA Kit
ab108666 Testosterone ELISA Kit Instructions for Use A competitive immunoenzymatic assay for the measurement of Testosterone in serum and plasma. quantitative This product is for research use only and
More informationab BNP 45 Rat ELISA Kit
ab108816 BNP 45 Rat ELISA Kit Instructions for Use For the quantitative measurement of Rat BNP 45 in plasma, serum, tissue extract, and cell culture supernatants. This product is for research use only
More informationab Transferrin Mouse ELISA Kit
ab157724 Transferrin Mouse ELISA Kit Instructions for Use For the quantitative measurement of Transferrin in Mouse serum and plasma samples. This product is for research use only and is not intended for
More informationab TNF alpha Human ELISA Kit
ab100654 TNF alpha Human ELISA Kit Instructions for Use For the quantitative measurement of Human TNF alpha in serum, plasma and cell culture supernatants. (Human TNF alpha concentration is pretty low
More informationab99974 ARP4 (Angiopoietin-Like 4) Human ELISA Kit
ab99974 ARP4 (Angiopoietin-Like 4) Human ELISA Kit Instructions for Use For the quantitative measurement of Human ARP4 in serum, plasma, and cell culture supernatants. This product is for research use
More informationab C Reactive Protein Mouse ELISA Kit
ab157712 C Reactive Protein Mouse ELISA Kit Instructions for Use For the quantitative measurement of C Reactive Protein in mouse serum, plasma and other biological samples. This product is for research
More informationab BNP 45 Rat ELISA Kit
ab108816 BNP 45 Rat ELISA Kit Instructions for Use For the quantitative measurement of Rat BNP 45 in plasma, serum, tissue extract, and cell culture supernatants. This product is for research use only
More informationab Anti-Mumps virus IgG Human ELISA Kit
ab108752 Anti-Mumps virus IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Mumps virus in Human serum and plasma (citrate). This product is for research
More informationab IL-1 Beta (Interleukin-1 Beta) Rat ELISA Kit
ab100768 IL-1 Beta (Interleukin-1 Beta) Rat ELISA Kit Instructions for Use For the quantitative measurement of rat IL-1 beta in cell lysates and tissue lysates. This product is for research use only and
More informationab Soluble TNF Receptor I Human ELISA Kit
ab100642 Soluble TNF Receptor I Human ELISA Kit Instructions for Use For the quantitative measurement of Human Soluble TNF Receptor I in serum, plasma and cell culture supernatants. This product is for
More informationab GDNF (Glial Derived Neurotrophic Factor) Human ELISA Kit
ab100525 GDNF (Glial Derived Neurotrophic Factor) Human ELISA Kit Instructions for Use For the quantitative measurement of Human GDNF in plasma, and cell culture supernatants. Notes: Human GDNF concentration
More informationab EGFR (ERBB) Human ELISA Kit
ab100505 EGFR (ERBB) Human ELISA Kit Instructions for Use For the quantitative measurement of Human EGFR in serum, plasma and cell culture supernatants. This product is for research use only and is not
More informationCancer Antigen CA19-9 Human ELISA Kit
ab108642 Cancer Antigen CA19-9 Human ELISA Kit Instructions for Use For the quantitative measurement of Human cancer antigen CA19-9 concentrations in serum This product is for research use only and is
More informationab Anti-Chlamydia pneumoniae IgA Human ELISA Kit
ab108719 Anti-Chlamydia pneumoniae IgA Human ELISA Kit Instructions for Use For the qualitative measurement of IgA class antibodies against Chlamydia pneumoniae in Human serum and plasma (citrate). This
More informationab Cortisol ELISA Kit
ab154996 Cortisol ELISA Kit Instructions for Use A competitive immunoenzymatic measurement of Cortisol in saliva. assay for the quantitative This product is for research use only and is not intended for
More informationab Cortisol ELISA Kit
ab154996 Cortisol ELISA Kit Instructions for Use A competitive immunoenzymatic measurement of Cortisol in saliva. assay for the quantitative This product is for research use only and is not intended for
More informationab Thrombin (Factor II) Human ELISA Kit
ab108909 Thrombin (Factor II) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Thrombin (Factor II) in cell culture supernatants. This product is for research use only and
More informationab Anti-Trichinella spiralis IgG Human ELISA Kit
ab108780 Anti-Trichinella spiralis IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Trichinella spiralis in Human serum and plasma (citrate). This
More informationab Anti-Varicella-Zoster virus (VZV) IgG Human ELISA Kit
ab108782 Anti-Varicella-Zoster virus (VZV) IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Varicella-Zoster virus (VZV) in Human serum and plasma
More informationab Porcine VEGF-A ELISA Kit
Version 1 Last updated 27 January 2017 ab218298 Porcine VEGF-A ELISA Kit For the quantitative measurement of porcine VEGF-A in serum, plasma and cell culture supernatants. This product is for research
More informationab Anti-Tick-borne encephalitis (TBE) virus IgG plus Human ELISA Kit
ab108763 Anti-Tick-borne encephalitis (TBE) virus IgG plus Human ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against Tick-borne encephalitis (TBE) virus in Human
More informationab VEGF Receptor 2 Human ELISA Kit
ab100665 VEGF Receptor 2 Human ELISA Kit Instructions for Use For the quantitative measurement Human VEGF Receptor 2 in serum, plasma and cell culture supernatants. This product is for research use only
More informationab Anti-Tick-borne encephalitis (TBE) virus IgG Human ELISA Kit
ab108773 Anti-Tick-borne encephalitis (TBE) virus IgG Human ELISA Kit Instructions for Use For the quantitative measurement of IgG class antibodies against Tick-borne encephalitis (TBE) virus in Human
More informationab OH Progesterone ELISA Kit
ab178608 17-OH Progesterone ELISA Kit Instructions for Use A competitive immunoenzymatic assay for measurement of 17-OH Progesterone in saliva. the quantitative This product is for research use only and
More informationab BNP 32 Rat ELISA Kit
ab108815 BNP 32 Rat ELISA Kit Instructions for Use For the quantitative measurement of Rat BNP 32 in plasma, serum, tissue extract, and cell culture supernatants. This product is for research use only
More informationab BNP 32 Rat ELISA Kit
ab108815 BNP 32 Rat ELISA Kit Instructions for Use For the quantitative measurement of Rat BNP 32 in plasma, serum, tissue extract, and cell culture supernatants. This product is for research use only
More informationab PF4 (CXCL4) Human ELISA Kit
ab100628 PF4 (CXCL4) Human ELISA Kit Instructions for Use For the quantitative measurement of Human PF4 (CXCL4) in serum, plasma, and cell culture supernatants. This product is for research use only and
More informationab beta Estradiol ELISA Kit
ab108667 17 beta Estradiol ELISA Kit Instructions for Use A competitive immunoenzymatic assay for the measurement of 17 beta Estradiol in serum and plasma. quantitative This product is for research use
More informationab Transferrin Human ELISA Kit
ab108902 Transferrin Human ELISA Kit Instructions for Use For the quantitative measurement of Human Transferrin in urine, milk saliva, CSF and cell culture supernatants. This product is for research use
More informationab Haptoglobin Canine ELISA Kit
ab137978 Haptoglobin Canine ELISA Kit Instructions for Use For the quantitative measurement of Canine Haptoglobin in plasma and cell culture supernatants. This product is for research use only and is not
More informationab BNP 32 Rat ELISA Kit
ab108815 BNP 32 Rat ELISA Kit Instructions for Use For the quantitative measurement of Rat BNP 32 in plasma, serum, tissue extract, and cell culture supernatants. This product is for research use only
More informationTissue Factor Human Chromogenic Activity Assay Kit
ab108906 Tissue Factor Human Chromogenic Activity Assay Kit Instructions for Use For the quantitative measurement of Human Tissue Factor activity in plasma, serum, urine, tissue and cell culture extracts
More informationab Serum Amyloid A (SAA) Human ELISA Kit
ab100635 Serum Amyloid A (SAA) Human ELISA Kit Instructions for Use For the quantitative measurement of Human SAA in serum, plasma and cell culture supernatants. This product is for research use only and
More informationab Polyethylene Glycol (PEG) ELISA Kit
ab138914 Polyethylene Glycol (PEG) ELISA Kit Instructions for Use For the competitive quantitative measurement of PEGylated molecules in plasma, serum and cell culture media. This product is for research
More informationFor the qualitative measurement of IgG class antibodies against Treponema pallidum in Human serum and plasma (citrate).
ab108779 Anti-Treponema pallidum IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Treponema pallidum in Human serum and plasma (citrate). This product
More informationab Adiponectin Human ELISA Kit
ab108786 Adiponectin Human ELISA Kit Instructions for Use For the quantitative measurement of Human Adiponectin in urine, plasma, serum, milk, saliva, and cell culture supernatants. This product is for
More informationab Albumin Human ELISA Kit
ab108787 Albumin Human ELISA Kit Instructions for Use For the quantitative measurement of Human Albumin in plasma and serum. This product is for research use only and is not intended for diagnostic use.
More informationab Anti-Entamoeba histolytica IgG Human ELISA Kit
ab108734 Anti-Entamoeba histolytica IgG Human ELISA Kit Instructions for Use For the qualitative measurement of IgG class antibodies against Entamoeba histolytica in Human serum and plasma (citrate). This
More informationab Complement C4 Human ELISA Kit
ab108824 Complement C4 Human ELISA Kit Instructions for Use For the quantitative measurement of Human Complement C4 in plasma and serum. This product is for research use only and is not intended for diagnostic
More informationab Anti-Brucella IgM Human ELISA Kit
ab108713 Anti-Brucella IgM Human ELISA Kit Instructions for Use For the qualitative measurement of IgM class antibodies against Brucella in Human serum and plasma (citrate). This product is for research
More informationab TNF alpha Rat ELISA Kit
ab108913 TNF alpha Rat ELISA Kit Instructions for Use For the quantitative measurement of TNF alpha in Rat cell culture supernatants. This product is for research use only and is not intended for diagnostic
More informationFor the quantitative measurement of human, mouse and rat phosphorylated STAT3 (Tyr705) concentrations in cell lysates.
ab126458 - STAT3 (py705) ELISA Kit Instructions for Use For the quantitative measurement of human, mouse and rat phosphorylated STAT3 (Tyr705) concentrations in cell lysates. This product is for research
More informationab Akt (ps473) + total Akt ELISA Kit
ab126433 - Akt (ps473) + total Akt ELISA Kit Instructions for Use For the quantitative measurement of human, mouse and rat phosphorylated Akt (Ser473) and total Akt concentrations in cell lysates. This
More informationab IL-1 beta (Interleukin-1 beta) Human ELISA Kit
ab108865 IL-1 beta (Interleukin-1 beta) Human ELISA Kit Instructions for Use For the quantitative measurement of Human IL-1 beta (Interleukin-1 beta) in plasma, serum and cell culture supernatants. This
More informationab Ferritin (FTL) Human ELISA Kit
ab108837 Ferritin (FTL) Human ELISA Kit Instructions for Use For the quantitative measurement of Human Ferritin (FTL) in plasma, serum, milk, cerebrospinal fluid and cell culture supernatants. This product
More informationab DNA-Protein Binding Assay Kit (Colorimetric)
ab117139 DNA-Protein Binding Assay Kit (Colorimetric) Instructions for Use For the measurement protein-dna interactions in vitro, specifically for detecting transcription factor activation using mammalian
More informationab ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit
ab126423 ERK1/2 (Thr202/Tyr204) In-Cell ELISA Kit Instructions for Use For the qualitative measurement of ERK1/2 (Thr202/Tyr204) phosphorylation in adherent Human, mouse and rat cell lines. This product
More informationab TrkA Rat ELISA Kit
ab119609 TrkA Rat ELISA Kit Instructions for Use For quantitative detection of rat TrkA in cell culture supernatants, cell lysates and tissue homogenates. This product is for research use only and is not
More informationab anti-adalimumab ELISA Kit (Humira )
Version 1 Last updated 23 August 2018 ab237657 anti-adalimumab ELISA Kit (Humira ) For the measurement of the antibody against Adalimumab in human serum and plasma. This product is for research use only
More informationHerpes Simplex Virus 1 (HSV 1) IgG Human ELISA Kit
ab108737 Herpes Simplex Virus 1 (HSV 1) IgG Human ELISA Kit Instructions for Use For the qualitative determination of IgG class antibodies against Herpes Simplex Virus 1 (HSV 1) in Human serum or plasma
More informationHerpes Simplex Virus 1 (HSV 1) IgM Human ELISA Kit
ab108738 Herpes Simplex Virus 1 (HSV 1) IgM Human ELISA Kit Instructions for Use For the qualitative determination of IgM class antibodies against Herpes Simplex Virus 1 (HSV 1) in Human serum or plasma
More information