mcherry Polyclonal Antibody Catalog Number PA Product data sheet

Transcription

1 Website: thermofisher.com Customer Service (US): ext. 1 Technical Support (US): ext. 441 mcherry Polyclonal Antibody Catalog Number PA Product data sheet Details Size 100 µl Host/Isotope Class Type Immunogen Conjugate Form Concentration Purification Storage buffer Contains Storage Conditions Rabbit / IgG Polyclonal Antibody Recombinant fragment contains a sequence corresponding to a region within amino acids 1 and 236 of mcherry Unconjugated Liquid 0.36 mg/ml Antigen affinity chromatography PBS, ph 7, with 30% glycerol, 0.5% BSA 0.01% thimerosal -20 C, Avoid Freeze/Thaw Cycles Species Reactivity Tested species reactivity Tag Published species reactivity Not Applicable Tested Applications Dilution * Immunocytochemistry (ICC) 1:100-1:1000 Immunofluorescence (IF) 1:100-1:1000 Immunoprecipitation (IP) 1:3000 Western Blot (WB) 1:1000-1:10,000 Published Applications Miscellaneous PubMed (MISC) See 1 publications below * Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls. Product specific information PA targets mcherry in ICC, IF, IP, and WB applications and shows reactivity with Human samples. The PA immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 1 and 236 of mcherry.

2 Product Images For mcherry Polyclonal Antibody (PA ) in IF Immunofluorescent analysis of mcherry in HeLa cells. mcherry fusion protientransfected HeLa cells were fixed in Triton X- 100 for 1 min, then 4% paraformaldehyde at RT for 30 min and stained using a mcherry polyclonal antibody (Product # PA ) diluted at 1:500. Red: mcherry is expressed in the tranfected cell. Blue: Hoechst staining. (PA ) in IF Immunofluorescent analysis of mcherry in 2 days-postfertilization zebrafish embryos. Embryos were stained using a mcherry polyclonal antibody (Product # PA ) diluted at 1:100. (PA ) in WB Western blot analysis of mcherry in Nontransfected (-) and PLK4 -transfected (+, inculding mcherry - tag) 293T whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with mcherry Polyclonal antibody (Product # PA ) at a dilution of 1:5000.

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4 (PA ) in WB Western blot analysis of mcherry using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of mcherry-tagged protein expressing 293T cells. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a mcherry polyclonal antibody (Product # PA ) at a dilution of 1:5000.

5 (PA ) in WB Western blot analysis of mcherry using A) 5ng recombinant mcherry (B) 10ng recombinant mcherry and C) 20ng recombinant mcherry. Samples were loaded onto a 12% SDS- PAGE gel and probed with a mcherry polyclonal antibody (Product # PA ) at a dilution of 1:2000. (PA ) in IP PA immunoprecipitates mcherry protein in IP experiments. IP Sample: 293T whole cell lysate/extract A: 20 µg whole cell lysate /extract of mcherry protein expressing 293T cells B: Control with 2.5 µg of preimmune rabbit IgG C: Immunoprecipitation of mcherry protein by 2.5 µg of mcherry antibody (Product # PA ) on 7.5% SDS-PAGE.

6 PubMed References For mcherry Polyclonal Antibody 1 Miscellaneous PubMed References Species / Dilution Summary PA was used in immunocytochemistry and western blot to describe a missense mutation that affects both functions of gephyrin Not Applicable / Not Cited EMBO molecular medicine ( 2015; 7: 1580) "Simultaneous impairment of neuronal and metabolic function of mutated gephyrin in a patient with epileptic encephalopathy." Author(s):Dejanovic B,Djémié T,Grünewald N,Suls A,Kress V,Hetsch F,Craiu D,Zemel M,Gormley P,Lal D,Myers CT,Mefford HC,Palotie A,Helbig I,Meier JC,De Jonghe P,Weckhuysen S,Schwarz G PubMed Article URL: