Mechanisms of extravascular destruction of red cells coated with IgG1 or IgG3 (± C3b).
|
|
- Amy Berry
- 6 years ago
- Views:
Transcription
1 Introduction - Antibodies involved in transfusion reactions are of two types, namely the complete and the incomplete. - whereas the complete antibodies agglutinate red cells in saline medium, the incomplete type of antibody sensitizes red cells without agglutination. IgM class of antibodies and IgG1 and IgG3 type of IgG antibodies fix complement. Cell lysis, in vivo, is mediated through the complement system and the complement component C3b is further acted upon to produce C3d.
2 Mechanisms of extravascular destruction of red cells coated with IgG1 or IgG3 (± C3b).
3 Antiglobulin Test (Anti-humanglobulin or AHG) Discovered by Coombs, Mourant and Race in 1945 who found that RBC can become sensitized without visible agglutination. AHG identifies IgG antibodies and Complement proteins
4 Principle of AGT The antiglobulin test (AGT) or Coombs test is based on the principle that antihuman globulins (AHGs) bind to human globulins such as IgG or complement, either free in serum or bound to RBC antigens. IgG antbodies are too small to cause direct agglutination of synthesised red blood cells. The addition of the AHG reagent (which contain anti-igg) to RBC synthesised with IgG antibodies allows for hemagglutination of theses synthesised RBC. Furthermore, AGT can detect complement synthesised RBC.
5 AHG REAGENT Anti human globulin is a balanced ready to use blend of highly purified immunoglobulins. It contains Anti human IgG antibodies and / or antibodies reactive with human complement components C3b and C3d.
6 AHG Antihuman globulin reagents may be polyspecific or monospecific. Polyspecific AHG contains antibody to human IgG and to the C3d component of human complement. (other anticomplement antibodies such as anti-c3b, and anti C4b may also be present). Monospecific AHG contain only one antibody specificity. (either anti-igg or anticomplement).
7 Preparation of AHG The classical method involves injecting human serum or purified globulin into lab animals, such as rabbits. The rabbit immune system react to the foreign Ag and produce antibody to human globulin. (AHG). This type of response produce a polyclonal antibodies recognise different epitopes.
8 Monoclonal antibodies -Hybridoma technology can be used to produce monoclonal antiglobulin serum. - Monoclonal antibodies are derived from one clone of plasma cells and recognise a single epitope.
9 Antiglobulin test In the direct antiglobulin tests (DAT), Anti human globulin reagent is used to detect antibodies adsorbed to the red blood cells in vivo. DAT is also called direct Coombs test. In the indirect antiglobulin tests (IAT), Anti human globulin reagent is used to detect antibodies adsorbed to the red blood cells in vitro. IAT is also known as indirct Coombs test.
10 AHG reagent will react with bound and/or free IgG and Complement proteins. Must get rid of the free antibodies in the serum to identify the bound forms that can cause hemolytic reaction. RBC should be washed properly before carrying out the AHG 2 Types of AHG test: 1. Direct antiglobulin test (DAT) 2. Indirect Antiglobulin test (IAT or antibody screen)
11 DAT Direct antiglobulin Test (DAT): Detects antibodies bound to RBC in vivo Example: new born baby RBC synthesised with maternal anti D. (+) DAT indicates an immune response; patients cells have attached IgG and/or Complement) EDTA is sample of choice for DAT Use of various drugs and certain diseases (such as megaloblastic anaemia) are known to be associated with a positive direct antiglobulin test.
12 DAT
13 Examples of diseases that give a positive direct Coombs test A positive Coombs test indicates that an immune mechanism is attacking the patient's own RBC's. This mechanism could be autoimmunity, alloimmunity or a drug-induced immune-mediated mechanism.
14 Examples of alloimmune hemolysis Hemolytic disease of the newborn (also known as HDN or erythroblastosis fetalis) Rh D hemolytic disease of the newborn (also known as Rh disease) ABO hemolytic disease of the newborn (the indirect Coombs test may only be weakly positive) Anti-Kell hemolytic disease of the newborn Rh c hemolytic disease of the newborn Rh E hemolytic disease of the newborn Other blood group incompatibility (RhC, Rhe, Kidd, Duffy, MN, P and others) Alloimmune hemolytic transfusion reactions
15 Examples of autoimmune hemolysis Warm antibody autoimmune hemolytic anemia Idiopathic Systemic lupus erythematosus Evans' syndrome (antiplatelet antibodies and hemolytic antibodies) Cold antibody autoimmune hemolytic anemia Idiopathic cold hemagglutinin syndrome Infectious mononucleosis Paroxysmal cold hemoglobinuria
16 Autoimmune & alloimmune haemolytic anaemia
17 Drug-induced immune-mediated hemolysis Methyldopa (IgG mediated type II hypersensitivity) Penicillin (high dose) Quinidine (IgM mediated activation of classical complement pathway and Membrane attack complex, MAC)
18 Drug-induced immune-mediated hemolysis
19 Indirect Antiglobulin Test (IAT) Detects in vitro sensitization of RBC 2 stage-test: 1. Incubation phase: incubation of test serum at 37 C with donor cells. 2. Antiglobulin Phase: Addition of Anti human globulin reagent into the cell mixture INTERPRETATION OF RESULTS: - Agglutination reported as positive result. - Positive IAT indicates a specific reaction between donor RBC antigen and antibody in the patient serum. - Sample of choice: IAT -Serum, not more than 48 hours old, should be used. -Complement dependent antibodies are not detected in plasma samples.
20
21 Applications of IAT 1- Antibody screening. 2-Cross matching 3- Antenatal antibody screening 4- determination of RBC phenotype using known antisera (eg., Kell typing, weak D testing).
22 DAT using the microcolumn (gel) system Gel microcolumn assay (GMA) has been shown to be more sensitive than the conventional tube test for detecting potentially significant antibodies coating red blood cells in vivo. See: Novaretti et al (2004) Positive Negative Journal of Clinical Laboratory Analalysis. 18: What is the principle of the gel method?
23 Advance techniques in Ag-antibody detection
24 Use of AHG test in HDN: Direct Coombs test: Fetus red cells and human IgGspecific antibody. Indirect Coombs test: Maternal serum, Rh+ red cells and humaniggspecific antibody
25 Red cell antigens Lutheran ABO Ii Gerbich Knops Diego Yt Duffy MNS Cromer Indian Kell Rh LW
Ain t Nobody Got Time for
Ain t Nobody Got Time for DATs Lindsay Peters MLS(ASCP)SBB Reference Laboratory Supervisor Mississippi Valley Regional Blood Center OBJECTIVES Define and describe the antiglobulin test. Identify the reagents
More informationAntibody - Antigen Reactions: ABO and D typing Antibody screening and identification
Antibody - Antigen Reactions: ABO and D typing Antibody screening and identification Basics of antigen/ antibody reactions Why is the ABO group so special? D antigen it s complicated! Antibody screen Antibody
More informationCompatibility Testing. Compatibility testing is performed to determine if a particular unit of blood can be transfused safely into a certain patient.
Compatibility Testing Compatibility testing is performed to determine if a particular unit of blood can be transfused safely into a certain patient. This includes ABO-Rh blood typing, antibody screening
More informationPRETRANSFUSION OR COMPATIBILITY TESTING
13 PRETRANSFUSION OR COMPATIBILITY TESTING 13.1 INTRODUCTION Pretransfusion compatibility testing serves to select a compatible unit of blood for the recipient which when transfused does not cause any
More informationVal Tunnard Sheffield RCI. Red Cell Immunohaematology
Val Tunnard Sheffield RCI Produced by the immune system Created in response to a foreign antigen Protein (Immunoglobulins) in serum/plasma Red cell antibodies are either: IgG, 2 binding site IgM, 10 binding
More informationRequirements of IBTO (AABB) Standards d for a Blood Transfusion Request 1.Positive identification of the recipient and the recipient blood samples.
Detection and Identification of alloantibodies to Red Cell Antigens Antibody Screen Test Iranian Blood Transfusion Organization Mostafa Moghaddam MS, CLS ( ASCP ) BB Head of Immunohematology Reference
More informationSkills: 20 points. Objectives:
Exercise 5 Type and Screen Skills: 20 points Objectives: 1. List three situations in which screening for unexpected antibodies should be performed. 2. List two patient populations who are candidates for
More informationUse and Abuse of Reagents and Techniques. Joann Moulds PhD, MT(ASCP)SBB Director, Scientific Support Services LifeShare Blood Centers Shreveport, LA.
Use and Abuse of Reagents and Techniques Joann Moulds PhD, MT(ASCP)SBB Director, Scientific Support Services LifeShare Blood Centers Shreveport, LA. Antibody Screening & Identification Blood Grouping Reference
More informatione-grips Module 18 Introduction to Antibody Identification
Introduction to Antibody Identification Introduction Objectives After completing the learning module, the learner will be able to: 1. Discuss the limitations of antibody detection and identification. 2.
More informationSusan T. Johnson, MSTM, MT(ASCP)SBB BloodCenter of Wisconsin Milwaukee, WI USA
Laboratory Investigation of Hemolytic Transfusion Reactions Speaker Professor A Pourazar Susan T Johnson, MSTM, MT(ASCP)SBB BloodCenter of Wisconsin Milwaukee, WI USA Laboratory Evaluation of Transfusion
More informationAntibody Identification: Art or Science? A Case Study Approach
Antibody Identification: Art or Science? A Case Study Approach (Questions and Answers) Editors Janis R. Hamilton, MS, MT(ASCP)SBB Susan T. Johnson, MSTM, MT(ASCP)SBB Sally V. Rudmann, PhD, MT(ASCP)SBB
More informationPRODUCT CATALOG. Immunohematology.
PRODUCT CATALOG Immunohematology www.biognost.com Complete dedication to science Fulfilling our ambition - first to detect, develop, and offer solutions Our everyday business is shaped by clear missions,
More informationTHANK YOU FOR SAVING MY LIFE
THANK YOU FOR SAVING MY LIFE Importance of blood typing Transfusion has taken an increasingly important role in the life support of canine and feline patients. Thus, the need for quick and accurate blood
More informationOriginal Equipment Manufacturing. Intermediate and OEM Products for Immunohaematology
Original Equipment Manufacturing Intermediate and OEM Products for Immunohaematology A long history as an OEM manufacturer For over 30 years, Quotient has developed, manufactured and marketed high quality
More information4/12/2019. Handouts Advanced Track Webinars. us/pages/educational Program Handouts.aspx
Handouts http://www.immucor.com/en us/pages/educational Program Handouts.aspx 2 All Content Immucor, Inc. 2019 Advanced Track Webinars Link to register: https://immucor.webinato.com/register 3 All Content
More informationRx ONLY
Blood Grouping Reagent Anti-D (Anti-Rh o ) (Human Monoclonal-Polyclonal Blend) BioClone For Rapid Tube, Slide and Microplate Tests Qualitative Test for Recognition of the D(Rh o ) Antigen on Human Red
More informationTools and Strategies Useful for Antibody Identification. Virginia Hare, MT(ASCP)SBB Immunohematology Reference Lab American Red Cross Southern Region
Tools and Strategies Useful for Antibody Identification Virginia Hare, MT(ASCP)SBB Immunohematology Reference Lab American Red Cross Southern Region 1 st case: 85 y.o. white female Admitted for a total
More informationSPECIALIST IN BLOOD BANKING EXPERIENCE DOCUMENTATION FORM (Routes 2, 3 & 4)
PART I (To be completed by Applicant) SPECIALIST IN BLOOD BANKING EXPERIENCE DOCUMENTATION FORM (Routes 2, 3 & 4) Applicant s Name Last Four Digits of Applicant s Social Security # Address E-mail Address
More informationImmunohematology Practicum Objectives - CLS 645
Clinical Laboratory Sciences Department University of Kansas Medical Center Immunohematology Practicum Objectives - CLS 645 The following objectives are to be completed by the student for successful completion
More informationINTERNATIONAL SPECIALIST IN BLOOD BANKING WORK EXPERIENCE DOCUMENTATION FORM (Routes 2 & 3)
PART I (TO BE COMPLETED BY APPLICANT) Applicant s Name Address Email Address PART II (MUST BE COMPLETED AND SIGNED BY LABORATORY MANAGEMENT* OR EMPLOYER IN ORDER TO BE ACCEPTABLE) SUBJECT: VERIFICATION
More informationINTERNATIONAL SPECIALIST IN BLOOD BANKING WORK EXPERIENCE DOCUMENTATION FORM (Routes 2 & 3)
PART I (TO BE COMPLETED BY APPLICANT) Applicant s Name Address Email Address PART II (MUST BE COMPLETED AND SIGNED BY LABORATORY MANAGEMENT* OR EMPLOYER IN ORDER TO BE ACCEPTABLE) SUBJECT: VERIFICATION
More informationAntibodies and Antigens in the Blood Bank 9/7/2015 NAHLA BAKHAMIS 1
Antibodies and Antigens in the Blood Bank NAHLA BAKHAMIS 9/7/2015 NAHLA BAKHAMIS 1 Outline Antibodies structure, classes and functions Most important Abs in the blood bank effective roles of Abs Zeta potential
More informationIs it time to re-evaluate the gold standard in red cell antigen typing?
Is it time to re-evaluate the gold standard in red cell antigen typing? Susan T. Johnson, MSTM, MT(ASCP)SBB American Society of Clinical Laboratory Science Central New England May 9, 2013 161 GLOBCC 8MAY2015
More informationSPECIALIST IN BLOOD BANKING WORK EXPERIENCE DOCUMENTATION FORM (Routes 2, 3, & 4)
PART I (TO BE COMPLETED BY APPLICANT) Applicant s Name Last Four Digits of Applicant s Social Security # Address Email Address Daytime Telephone Number PART II (MUST BE COMPLETED AND SIGNED BY THE IMMEDIATE
More informationSPECIALIST IN BLOOD BANKING WORK EXPERIENCE DOCUMENTATION FORM (Routes 2, 3, & 4)
PART I (TO BE COMPLETED BY APPLICANT) Applicant s Name Last Four Digits of Applicant s Social Security # Address Email Address Daytime Telephone Number PART II (MUST BE COMPLETED AND SIGNED BY THE IMMEDIATE
More informationRed Cell Immunohaematology. Mark Dwight Specialist Biomedical Scientist RCI Filton
Red Cell Immunohaematology Mark Dwight Specialist Biomedical Scientist RCI Filton The RCI Laboratories Red Cell Immunohaematology Reference laboratories supporting hospital blood banks in England. RCI
More informationBLOOD TYPING REAGENTS. Product Profile
BLOOD TYPING REAGENTS Product Profile 2 BLOOD TYPING REAGENTS Its all about Human Blood Human blood is classified based on presence or absence of inherited antigens on the surface of red blood cells (RBCs).
More informationAntibodies and Antigens In the blood bank
Antibodies and Antigens In the blood bank 1 Nice game!! http://nobelprize.org/ 2 Karl Landsteiner discovered blood groups in 1901. Awarded Nobel Prize for Physiology or Medicine in 1930 3 Why we study
More informationThis chapter describes
11 Red Cell Antigen- Antibody Reactions and Their Detection This chapter describes the three major types of antigen-antibody reactions, the factors that affect agglutination reactions, the antiglobulin
More informationBlood Grouping Reagent IH-Anti-D (RH1) Blend
Blood Grouping Reagent IH-Anti-D (RH1) Blend English, B186530, Version 07, 2018.11 FOR IN VITRO DIAGNOSTIC USE Anti-D reagent for use with IH-System MEETS FDA POTENCY REQUIREMENTS U.S. License Number:
More informationSanquin Blood Grouping Reagents
Sanquin Blood Grouping Reagents Blood grouping reagents are essential tools for diagnostic laboratories specialised in serology. The broad range of reagents developed by Sanquin Reagents is used in several
More informationResolve Panel A A Qualitative Test for the Identification of Unexpected Blood Group Antibodies
Revised October 2015 Reagent Red Blood Cells Resolve Panel A A Qualitative Test for the Identification of Unexpected Blood Group Antibodies 719510 Rx ONLY SUMMARY AND EXPLANATION When an unexpected antibody
More informationGeorgia Spanos, MT LifeSouth Community Blood Centers Gainesville, Fl. SEABB March 2017
Georgia Spanos, MT LifeSouth Community Blood Centers Gainesville, Fl. SEABB March 2017 Case Study 1 Patient Demographics -44 year old -African-American Female -Diagnosis: Gastroenteritis -Facility reported
More informationJaundiced Baby. Introduction to the Patient-Oriented Problem-Solving (POPS) System
Jaundiced Baby Developed by Larry J. McCumber, PhD Parker A. Small, Jr, MD Department of Immunology and Medical Microbiology College of Medicine University of Florida Gainesville, Florida Modified by G.
More informationCeftriaxone induce immune hemolytic anemia in Sudanese patients
Ceftriaxone induce immune hemolytic anemia in Sudanese patients Tashreeg Awad elgied elamiean Ahmed, Leena babiker mirghani Faculty of medical Laboratory Sciences, Alneelin University, Khartoum, Sudan
More informationBlood Bank Review. Case #1 ABO/Rh. Case #1 Antibody Screen. Is the ABO grouping discrepant? No Interpretation: O positive
Blood Bank Review Mary Burroughs Blood Bank Technical Specialist Mercy Health Saint Mary s Case #1 ABO/Rh Forward Grouping Reverse Grouping Anti-A Anti-B Anti-D A1 cells B cells 0 0 3+ 4+ 4+ Is the ABO
More informationGroup A, B, Oh No! When there is an ABO typing discrepancy CYNTHIA CRUZ, MLS(ASCP) CM American Red Cross Reference
Group A, B, Oh No! When there is an ABO typing discrepancy CYNTHIA CRUZ, MLS(ASCP) CM American Red Cross Reference Objectives To correlate the forward ABO typing with the reverse ABO typing Determine where
More informationDTT Treated Reagent Red Cells for use in Resolving DARA Interference. More Than Just Kell?? Marilyn Stewart MT(ASCP)SBB
DTT Treated Reagent Red Cells for use in Resolving DARA Interference More Than Just Kell?? Marilyn Stewart MT(ASCP)SBB Daratumumab Anti-myeloma and anti-lymphoma agent known to interfere with routine Blood
More informationDefinitions. Absorption/Adsorption and Elution. Elution. CLS 422 Clinical Immunohematology I Absorption and Elution 1
Definitions Absorption/Adsorption and Elution CLS 422 Clinical Immunohematology I Absorption: To remove antibodies from serum through the action of the target antigen. Adsorption: The uptake of antibody
More informationDARATUMUMAB HARD TO PRONOUNCE - EVEN HARDER ON BLOOD BANK TESTING. Dr. Jennifer Duncan Hematopathology Resident March 18, 2017
DARATUMUMAB HARD TO PRONOUNCE - EVEN HARDER ON BLOOD BANK TESTING Dr. Jennifer Duncan Hematopathology Resident March 18, 2017 Goals and Objectives: Briefly describe the use of dara in the treatment of
More informationFORENSIC SEROLOGY. Chapter PRENTICE HALL 2008 Pearson Education, Inc. Upper Saddle River, NJ 07458
Chapter 8 FORENSIC SEROLOGY 8-1 Nature of Blood The word blood refers to a highly complex mixture of cells, enzymes, proteins, and inorganic substances. Plasma, which is the fluid portion of blood, is
More informationVII. Rh Blood Group System
VII. Rh Blood Group System A. The Rh system is one of the most complex genetic systems, and certain aspects of its genetics, nomenclature and antigenic interactions are unsettled. The aim of this lecture
More informationVIII. Pretransfusion Compatibility Testing
VIII. Pretransfusion Compatibility Testing A. The purpose of pretransfusion testing is to select, for each recipient, blood components that, when transfused, will have acceptable survival and will not
More informationFor order enquiries. Telephone Fax For technical information
Sanquin is responsible for safe and efficient blood supply in the Netherlands on a not-for-profit basis. Sanquin also develops and produces pharmaceutical products, conducts high-quality scientific research,
More informationSTANDARD OPERATING PROCEDURE FOR ANTIBODY IDENTIFICATION - TUBE METHOD
STANDARD OPERATING PROCEDURE FOR ANTIBODY IDENTIFICATION - TUBE METHOD TITLE: STANDARD OPERATING PROCEDURE FOR ANTIBODY IDENTIFICATION TUBE METHOD 1.0 Principle An antibody identification procedure is
More informationGEL CARD AND SALINE TUBE TECHNIQUES FOR BLOOD CROSS MATCHING: A COMPARATIVE ASSESSMENT
GEL CARD AND SALINE TUBE TECHNIQUES FOR BLOOD CROSS MATCHING: A COMPARATIVE ASSESSMENT Mahamat Ahmat Aboussakine 1, Dr. Kalpana Sankhala 2 1 MSc Blood Transfusion Technologym. NIMS University,Jaipur Rajasthan,
More informationBIOSCOT Blood Typing Monoclonal Reagents
BIOSCOT Blood Typing Monoclonal Reagents Merck Millipore, your partner for monoclonal blood typing diagnostics For over 25 years Merck Millipore has been leading the development of monoclonal antibodies
More informationSerological Interference Caused by Monoclonal Antibody Drug Therapy
Serological Interference Caused by Monoclonal Antibody Drug Therapy Presented by: Christina Barron, MT(ASCP)SBB Prepared by: Christina Barron, MT(ASCP)SBB Monica A. Keith MS, BB(ASCP) Kelly Kezeor MT(ASCP)
More informationDiscussion. Principle. Laboratory Procedure Manual EXERCISE 3. Blaney, Chapter 4 The ABO and H Blood Group Systems. Textbook: 20 points.
Exercise 3 ABO and D Typing Textbook: Blaney, Chapter 4 The ABO and H Blood Group Systems Skills: 20 points Objectives 1. Explain why the ABO blood groups are the most significant of all blood group systems.
More informationAntigens & Antibodies II. Polyclonal antibodies vs Monoclonal antibodies
A Brief Review of Antibody Structure A Brief Review of Antibody Structure The basic antibody is a dimer of dimer (2 heavy chain-light chain pairs) composed of repeats of a single structural unit known
More informationRed cell alloantibodies
17 Identification of Alloantibodies to Red Cell Antigens Red cell alloantibodies other than non-red-cell-stimulated anti-a or -B are called unexpected red cell alloantibodies and may be found in 0.3-38%
More informationFalse-positive antiglobulin tests in healthy subjects and in hospital patients
Journal of Clinical Pathology, 1979, 32, 1014-1018 False-positive antiglobulin tests in healthy subjects and in hospital patients J. FREEDMAN From the Blood Bank, St Michael's Hospital, University of Toronto,
More informationAcceptable Student Result Range Identify with 100% accuracy any alloantibody in a patient serum sample.
Name Panel Studies Principle If positive reactions (agglutination and/or hemolysis) are obtained at any phase of the antibody screen or crossmatch procedure, a panel study must be performed. Positive reactions
More informationWhen, autoimmune hemolytic anemia
IMMUNOHEMATOLOGY Adsorption of autoantibodies in the presence of LISS to detect alloantibodies underlying warm autoantibodies Jacques Chiaroni, Mohammed Touinssi, Magali Mazet, Philippe de Micco, and Virginie
More information3/3/2013. Reference Lab Case Studies
Reference Lab Case Studies Virginia Hare, MT(ASCP)SBB American Red Cross Southern Region Douglasville, Georgia Today s Cases and Presenters Rh typing discrepancy by Grace Lee An Unusual Phenotype: Getting
More informationReference Lab Case Studies. Virginia Hare, MT(ASCP)SBB American Red Cross Southern Region Douglasville, Georgia
Reference Lab Case Studies Virginia Hare, MT(ASCP)SBB American Red Cross Southern Region Douglasville, Georgia Today s Cases and Presenters Rh typing discrepancy by Grace Lee An Unusual Phenotype: Getting
More informationAntigen-Antibody. reactions (2) By: Masheal Aljumaah OCT 2018
Antigen-Antibody reactions (2) By: Masheal Aljumaah OCT 2018 Learning objectives: introduction to Antigen Antibody reactions. Antigen Antibody reactions part1: Precipitation, Flocculation and Immunodiffusion.
More informationApplicable To Employees of the Gundersen Palmer Lutheran Hospitals and Clinics laboratories.
Subject ABO Forward Grouping - D Antigen Typing - Palmer Index Number Lab-8791 Section Laboratory Subsection Regional/Affiliates Category Departmental Contact Betty Tilleraas Last Revised 10/31/2017 References
More informationSerological Tools For Investigating Problems In Immunohaematology
Serological Tools For Investigating Problems In Immunohaematology Dr.K.Vasantha Deputy Director (Retd.) National Institute of Immunohaematology (ICMR) Parel, Mumbai Immunohaematology is an application
More informationSo we can separate antigens into their components and allow them to react with their antibodies
Ag-ab reactions As for single immunodiffusion, double immunodiffusion can be also combined with electrophoresis to speed up the reaction, and in this case the test is called immunoelectrophoresis. So electrophoresis
More informationTable of Contents. Preface... xv. Editorial Comments... xvii. About the Authors... xix
Preface................................................................... xv Editorial Comments........................................................ xvii About the Authors..........................................................
More informationCase Report: Atypical Presentation of Anti-Rg a
100 Case Report: Atypical Presentation of Anti-Rg a Eiad Kahwash, Sameer S.Talwalkar, Jill Leonard, and William Lockwood Department of Pathology and Laboratory Medicine, University of Louisville, Louisville,
More informationIt s in the Details Problems with Blood Bank Testing You Never Saw Coming
It s in the Details Problems with Blood Bank Testing You Never Saw Coming Heart of America April 17, 2018 Presented by Rebecca Bullock, MT(ASCP)SBB Technical Director, Quotient Biodiagnostics I have come
More informationAntigen-Antibody reactions
Antigen-Antibody reactions Ag Ab reactions in vitro are known as Serological reactions. Help in :- 1. In the diagnosis of infections 2. Epidemiological surveys 3. Identification of infectious and noninfectious
More informationBlood Grouping Reagent IH-Card Group ABO A-B-A-B-A-B
Blood Grouping Reagent IH-Card Group ABO A-B-A-B-A-B English, B186363, Version 06, 2017.03 FOR IN VITRO DIAGNOSTIC USE Gel card for use with the IH-System MEETS FDA POTENCY REQUIREMENTS U.S. LICENSE NUMBER:
More informationSafety Data Sheets for Blood Bank Products
Safety Data Sheets for Blood Bank Products Dear Valued Customer: Ortho Clinical Diagnostics (OCD) provides Safety Data Sheets (SDS) for any OCD products that contain hazardous materials at or above regulated
More informationExpertise. Specialty Products Complete the puzzle with the industry s most comprehensive line of products for transfusion diagnostics.
Expertise. Specialty Products Complete the puzzle with the industry s most comprehensive line of products for transfusion diagnostics. For 30 years, Immucor has been a dedicated partner to your Blood Bank
More informationDr Priti Elhence, Professor Dept of Transfusion Medicine, SGPGIMS MD (Transfusion Medicine), SGPGI Sr Residency Faculty Since 2000
CV Dr Priti Elhence, Professor Dept of Transfusion Medicine, SGPGIMS MD (Transfusion Medicine), SGPGI -1991-94 Sr Residency 1994 97 Faculty Since 2000 Princess of Wale fellowship Areas of interest Immunohematology,
More informationCLINICAL SIGNIFICANCE:
Subject Crossmatch Procedure Gel Method Index Number Lab-8769 Section Laboratory Subsection Regionals/Affiliates Category Departmental Contact Rachel Blum Last Revised 9/7/2017 References Required document
More informationDiagnostic Microbiology
Diagnostic Microbiology 320 MIC Lecture: 4 Identification of Microbes 3/8/2014 1 Agglutination and Precipitation Reactions Agglutination testing: Antibody cross links whole-cell antigens, forming complexes
More informationGUIDELINES FOR PRETRANSFUSION TESTING : PART I
Commissie voor Klinische Biologie Commission de Biologie Clinique GUIDELINES FOR PRETRANSFUSION TESTING : PART I Dr. A. Vanhonsebrouck, Bloedtransfusiecentrum Antwerpen 26-03-2009 WIV congress Rue Juliette
More informationA New Reagent (ZZAP) Having Multiple Applications in Immunohema tology
A New Reagent () Having Multiple Applications in Immunohema tology DONALD R. BRANCH, MT(ASCP)SBB AND LAWRENCE D. PETZ, M.D. A reagent () containing a mixture of.1 M dithiothreitol (DTT) plus.1% cysteine-activated
More informationThe complement components are present in serum in inactive form.
Lab 3 complement A complex consisting of at least 20 serum proteins, which, once activated, Its lytic activity is destroyed when heated at 56ᵒC for 30 min. acts like a part of the innate immune defense.
More informationSerological Testing: Why we did what we did!
Serological Testing: Why we did what we did! W. John Judd, FIBMS, MIBiol Emeritus Professor University of Michigan What we did Provided blood and blood products in a timely, cost-efficient manner. Implemented
More informationBlood Grouping Reagent and Anti-Human Globulin Anti-IgG,-C3d; Polyspecific IH-Card ABO/RhD (DVI+) A-B-A,B-D (DVI+)-Ctl-AHG
Blood Grouping Reagent and Anti-Human Globulin Anti-IgG,-C3d; Polyspecific IH-Card ABO/RhD (DVI+) A-B-A,B-D (DVI+)-Ctl-AHG English, B186360, Version 07, 2017.12 FOR I VITRO DIAGOSTIC USE Gel card for use
More informationROLE OF MOLECULAR GENOTYPING IN TRANSFUSION MEDICINE
ROLE OF MOLECULAR GENOTYPING IN TRANSFUSION MEDICINE Dr.( Prof.) R.N. Makroo Director and Sr. Consultant Dept. of Blood Transfusion Medicine, Molecular Biology & Transplant Immunology Indraprastha Apollo
More informationABO Typing Discrepancies
Jayme Heimonen MT(ASCP)SBB Blood Bank Technical Specialist Borgess Medical Center Kalamazoo, MI ASCLS-Michigan 2016 1 Expected reactions are missing 4+ 0 4+ 0 0 0 0 0 4+ 0 0 3+ 2 Unexpected reactions are
More informationCombining Serology and Genotyping to Improve Patient Outcomes: Case Studies
Combining Serology and Genotyping to Improve Patient Outcomes: Case Studies Lauro E. Guerra, Jr., BS, MLS(ASCP) CM SBB CM Manager, Grifols Immunohematology Center, San Marcos, TX, USA March 22, 2019 Objectives
More informationApplications of Red Cell Genotyping in Serological Problem-Solving
Applications of Red Cell Genotyping in Serological Problem-Solving Philippe P Pary RT, MT(ASCP)SBB Transfusion Medicine Children s National Health System Molecular vs Serological Phenotypes Serological
More information6/15/2015. Molecular vs Serological Phenotypes. Molecular vs Serological Phenotypes. Molecular Phenotyping Advantages
Applications of Red Cell Genotyping in Serological Problem-Solving Philippe P Pary RT, MT(ASCP)SBB Transfusion Medicine Children s National Health System Molecular vs Serological Phenotypes Serological
More informationCarol A Starks MS, MLS(ASCP), CLS. castarks
Carol A Starks MS, MLS(ASCP), CLS Brief review of the common blood groups ABO Rh Kell Duffy Kidd P Lutheran MNS Lewis Basic Blood Bank workups ABO discrepancies Single antibody not demonstrating Single
More informationWeak and Partial D phenotypes
Weak and Partial D phenotypes A perinatal testing laboratory approach Dr. Gwen Clarke Vein to Vein Calgary March 2016 Conflicts of interest: Travel funds and honoraria RedMedEd 2 Current Methods: CBS perinatal
More informationBlood Physiology. Blood Physiology. Dr. Rodolfo T. Rafael. Agglutination Hemolysis Immunology
Blood Physiology Dr. Rodolfo T. Rafael 1 Blood Physiology Agglutination Hemolysis Immunology 2 1 Agglutination is an antigen- antibody reaction Blood clumping of red blood cells Blood Typing 3 BLOOD TYPING
More informationRCPAQAP TRANSFUSION TRANSFUSION COMPETENCY PROGRAM METHOD CLASSIFICATION MASTERLIST
A.B.N: 32 003 520 072 RCPAQAP TRANSFUSION TRANSFUSION COMPETENCY PROGRAM METHOD CLASSIFICATION MASTERLIST 2013 Before 1 July 2013 After 1 July 2013 RCPAQAP Transfusion RCPAQAP Centre Of Excellence PO Box
More informationABO Blood Group 9/13/2015 NAHLA BAKHAMIS MSC 1
ABO Blood Group NAHLA BAKHAMIS MSC. 9/13/2015 NAHLA BAKHAMIS MSC 1 Glossary Chromosome Gene Locus Allele Heterozygous Homozygous Phenotype Genotype Sex Chromosomes Autosomal Chromosomes Dominant Gene Recessive
More informationCURRENT COURSE OFFERINGS
The American Red Cross offers regular educational opportunities as a convenient way for healthcare providers to receive relevant blood banking and transfusion medicine information. The bi-monthly sessions,
More informationWe are actually talking about the Rh Blood Group System!
hr Problems Is this a discussion of your Human Resources nightmares? We are actually talking about the Rh Blood Group System! hr Problems 1 Hr and hr Antigens Hr o (Rh17) Hr or Hr S (Rh18) Hr B (Rh34)
More informationPractical Applications of Immunology (Chapter 18) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Eastern Campus
Practical Applications of Immunology (Chapter 18) Lecture Materials for Amy Warenda Czura, Ph.D. Suffolk County Community College Eastern Campus Primary Source for figures and content: Tortora, G.J. Microbiology
More informationSTANDARD OPERATING PROCEDURE FOR QUALITY CONTROL OF REAGENTS AND ANTISERA
STANDARD OPERATING PROCEDURE FOR QUALITY CONTROL OF REAGENTS AND ANTISERA TITLE: STANDARD OPERATING PROCEDURE FOR QUALITY CONTROL OF REAGENTS AND ANTISERA 1.0 Principle 1.1 The purpose of quality control
More informationBlood Grouping Reagent IH-Card Anti-D (DVI+) D-D-D-D-D-D
Blood Grouping Reagent IH-Card Anti-D (DVI+) D-D-D-D-D-D English, B186368, Version 07, 2018.11 FOR IN VITRO DIAGNOSTIC USE Gel card for use with the IH-System MEETS FDA POTENCY REQUIREMENT U.S. LICENSE
More informationImmunoglobulins have protective functions which enable the living organism to fight multiple different infections.
Immunology 8 Immunoglobulins properties 20-6-2013 **Those are just extra notes for Immunoglobulins properties slides Introduction: Immunoglobulins have protective functions which enable the living organism
More informationWorking Experience with Solid Phase Antibody Detection Technique
Rounds [Blood Bank/Transfusion Medicine] Working Experience with Solid Phase Antibody Detection Technique Anjali A. Satoskar, MD, David Krugh, MT(ASCP)SBB, CLS(NCA), Melanie S. Kennedy, MD Department of
More informationObservations about complement were carried out by Nuthall Pfeiffer and Bordet in the 1800's.
COMPLEMENT SYSTEM Observations about complement were carried out by Nuthall Pfeiffer and Bordet in the 1800's. Researchers compared cholera vibrio with immune fresh serum in the test tube and; Cholera
More informationType and screen policy in the blood bank: Is AHG cross-match still required? A study at a multispecialty corporate hospital in India
Original Article Type and screen policy in the blood bank: Is AHG cross-match still required? A study at a multispecialty corporate hospital in India Sangeeta Pathak, M. Chandrashekhar 1, G. R. Wankhede
More informationMLAB IMMUNOHEMATOLOGY
Course Syllabus MLAB 2431- IMMUNOHEMATOLOGY Catalog Description: A study of blood antigens and antibodies. Presents quality control, basic laboratory technique and safety. Includes the principles, procedures
More informationRed cell immunology and compatibility testing
CHAPTER 16 Red cell immunology and compatibility testing Connie M. Westhoff Laboratory of Immunohematology and Genomics, New York Blood Center, New York, NY, USA Introduction Immunohematology is the study
More informationAnti-Human Globulin Anti-IgG,-C3d; Polyspecific IH-Card AHG Anti-IgG,-C3d (Rabbit / Murine Monoclonal)(Green)
Anti-Human Globulin Anti-IgG,-C3d; Polyspecific IH-Card AHG Anti-IgG,-C3d (Rabbit / Murine Monoclonal)(Green) English, B186358, Version 11, 2019.04 FOR I VITRO DIAGOSTIC USE Gel card for use with the IH-System
More informationRCPAQAP TRANSFUSION GENERAL TRANSFUSION PROGRAM METHOD CLASSIFICATION MASTERLIST
A.B.N. 32 003 520 072 RCPAQAP TRANSFUSION GENERAL TRANSFUSION PROGRAM METHOD CLASSIFICATION MASTERLIST 2013 Before 1 July 2013 After 1 July 2013 RCPAQAP Transfusion RCPAQAP Centre Of Excellence PO Box
More informationAnti-Human Globulin Anti-IgG IH-Card AHG Anti-IgG (Rabbit)(Green)
Anti-Human Globulin Anti-IgG IH-Card AHG Anti-IgG (Rabbit)(Green) English, B186359, Version 10, 2018.11 FOR I VITRO DIAGOSTIC USE Gel card for use with the IH-System MEETS FDA POTECY REQUIREMETS U.S. LICESE
More informationBlood Typing Monoclonal Antibodies. Blood typing intermediates for further manufacturing use
Blood Typing Monoclonal Antibodies Blood typing intermediates for further manufacturing use Merck Millipore, your partner for monoclonal blood typing antibodies For more than 25 years we have been leading
More information