BioBank TM cdna Kit. Instructions for the use of BioBank TM cdna in real-time PCR. BioBank control cdna
|
|
- Ralf Mills
- 6 years ago
- Views:
Transcription
1 BioBank TM cdna Kit Instructions for the use of BioBank TM cdna in real-time PCR BB BioBank control cdna
2 Contents Introduction 3 Kit Contents 4 Reagents and Equipment to Be Supplied by User 4 PrimerDesign Satisfaction Guarantee 5 Quality Control 5 Bench-side Protocol 6 Amplification Protocol 8 2
3 Introduction The BioBank TM a high quality source of cdna validated for use in real-time PCR experiments. The cdna is reverse transcribed from high quality DNAse treated RNA from a variety of sources using an optimised blend of Oligo-dT and random nonamer primers. BioBank TM cdna is therefore essentially free of genomic DNA and PCR inhibitors and covers the widest possible range of RNA and mrna transcripts in the specified tissue or cell line. BioBank TM cdna is useful for expression profiling of newly identified genes, and also as a positive control for the real-time PCR step. Positive control Primers Positive control primers and probe are supplied with the kits which detect 18S ribosomal RNA. These primers include a PerfectProbe TM for optimal probe based detection through the FAM channel. However the positive control primers and probe mix can also be used with standard Taqman cycling conditions and with SYBRgreen mastermix for detection using SYBR green chemistry. The positive control primers will give a cycle threshold value of approximately CT=12 depending on the detection chemistry and the particular hardware settings in use. 3
4 Kit Contents BioBank TM cdna 250ng (PURPLE PURPLE) Control primers and probe for 18S ribosomal RNA (BROWN BROWN) RNAse/ NAse/DNAse free water Reagents and Equipment to Be Supplied by User Real-Time PCR instrument Mastermix or Mastermix components This kit is designed to work well with all commercially available Mastermixes. However, we recommend the use of PrimerDesign 2x Precision TM Mastermix. Pipettors and Tips Vortex and centrifuge Kit Storage The PrimerDesign BioBank TM cdna kit should be stored at -20ºC on arrival. Freeze/thawing cycles should be kept to a minimum once resuspended. Under these conditions reagents are stable for six months from date of resuspension. 4
5 PrimerDesign Satisfaction Guarantee PrimerDesign takes pride in the quality of all of our products. Should this product fail to perform satisfactorily when used according to the protocols in this manual, PrimerDesign will replace the item free of charge. Quality Control As part of our routine quality assurance programme, all PrimerDesign products are monitored to ensure the highest levels of performance and reliability. 5
6 Bench-side Protocol 1. Pulse-spin spin each tube in a centrifuge before opening. This will ensure lyophilised primer and probe mix is in the base of the tube and is not spilt upon opening the tube. 2. Resuspend lyophilised primer and probe mix and cdna c in RNAse/DNAse free water provided according to the table below; To ensure complete resuspension, vortex each tube thoroughly, allow to stand for 5 minutes, and vortex again before use. Component Volume BioBank TM cdna (PURPLE PURPLE) 130 µl 18S control primers and probe (BROWN BROWN) 50 µl Real-time PCR 1. Positive control with 18S Make up a well in duplicate containing the positive control primers and BioBank TM cdna Component 1 Reaction Precision TM Mastermix (not included) 10 µl 18S primers and probe (BROWN BROWN) 1 µl BioBank TM cdna (PURPLE PURPLE) 5 µl RNAse/DNAse free water (WHITE WHITE) 4 µl Final volume 20 µl 2. Target gene with PrimerDesign custom designed real-time PCR assay (optional step) Make up a well in duplicate containing the positive control primers and BioBank TM cdna Component 1 Reaction Precision TM Mastermix (not included) 10 µl Target gene primer mix 1 µl BioBank TM cdna (PURPLE PURPLE) 5 µl RNAse/DNAse free water (WHITE WHITE) 4 µl Final volume 20 µl 6
7 3. Target gene with experimental primers (optional step) Make up a well in duplicate containing the experimental primers and BioBank TM cdna Component 1 Reaction Precision TM Mastermix (not included) 10 µl Forward primer 6pmol Reverse primer 6pmol Probe (optional) 3pmol BioBank TM cdna (PURPLE PURPLE) 5 µl RNAse/DNAse free water (WHITE WHITE) X µl Final volume 20 µl 7
8 Amplification Protocol The 18S control primers are specifically formulated to work under all recommended realtime PCR cycling conditions. i.e. Taqman cycling, PerfectProbe TM cycling, and SYBR green cycling conditions will all work perfectly with the kit. Amplification conditions using PerfectProbe TM cycling settings Cycling x50 Step Time Temp Enzyme activation 10min 95ºC Denaturation 15s 95ºC DATA COLLECTION* 30s 50ºC Extension 15s 72ºC *Fluorogenic data should be collected during this step through the FAM channel. Amplification conditions using Taqman or SYBR green cycling settings Cycling x50 Step Time Temp Enzyme activation 10min 95ºC Denaturation 15s 95ºC DATA COLLECTION* 60s 60ºC *Fluorogenic data should be collected during this step through the FAM or SYBR channel. 8
BioBank cdna kit. Instructions for the use of BioBank control cdna in real-time PCR
BioBank cdna kit Instructions for the use of BioBank control cdna in real-time PCR Contents Introduction 3 Kit contents 4 Reagents and equipment to be supplied by user 4 Kit storage and stability 4 Primerdesign
More informationReference gene detection assay. Instructions for detection and quantification of a reference gene using SYBR Green detection chemistry
Reference gene detection assay Instructions for detection and quantification of a reference gene using SYBR Green detection chemistry Contents Introduction 3 Kit contents 4 Kit storage 4 Reagents and equipment
More informationCopy number standard curve. for absolute quantification by real-time PCR
Copy number standard curve for absolute quantification by real-time PCR Kit contents Positive control template for standard curve (RED) Template preparation buffer (YELLOW) RNase/DNase free water (WHITE)
More informationEquus caballus Mitochondrial DNA quantification
PrimerDesign Ltd GMO event quantification Equus caballus Mitochondrial DNA quantification Detection and quantification of Equus caballus mitochondrial DNA by real-time PCR Species Specific Detection detection
More informationoasig TM lyophilised 2X qpcr Mastermix
oasig TM lyophilised 2X qpcr Mastermix Instructions for use of Primerdesign oasig lyophilised Mastermix MM MasterMix Contents Introduction 3 Kit Contents 4 Kit Storage 4 Suitable Sample Material 4 Licensing
More informationPrecision TM Reverse Transcription Premix 2 Reverse transcription using pre mixed components. RT Reverse Transcription
Precision TM Reverse Transcription Premix 2 Reverse transcription using pre mixed components RT Reverse Transcription Contents Introduction 3 Kit Contents 4 Recommended Accompanying Products 4 Reagents
More informationgenorm TM Reference Gene Selection Kit
genorm TM Reference Gene Selection Kit For the establishment of the optimal reference genes using SYBR green detection chemistry GE genorm reference gene selection Contents Introduction 3 Kit Contents
More informationHuman Y-Chromosome detection assay
Human Y-Chromosome detection assay Instructions for the quantification of the human Y-Chromosome by real-time PCR using a Double-Dye Probe CTRL Internal controls and gdna Contents Introduction 3 Kit Contents
More informationInternal RNA extraction control. Instructions for use of RNA real-time PCR internal extraction control kit
Internal RNA extraction control Instructions for use of RNA real-time PCR internal extraction control kit Contents Introduction 3 Kit contents 4 Reagents and equipment to be supplied by user 4 Kit storage
More informationInternal RNA extraction control. Instructions for use of RNA real-time PCR internal extraction control kit
Internal RNA extraction control Instructions for use of RNA real-time PCR internal extraction control kit Contents Introduction 3 Kit contents 4 Reagents and equipment to be supplied by user 4 Kit storage
More informationMaize Bt-176 quantification
Primerdesign Ltd GMO event quantification Maize Bt-176 quantification Detection and quantification of GMO integration events by real-time PCR GMO screening Event specific detection by real-time PCR Contents
More informationHuman Y chromosome detection assay. Detection of the human Y chromosome by real-time PCR
Human Y chromosome detection assay Detection of the human Y chromosome by real-time PCR Contents Kit contents 3 Reagents and equipment to be supplied by user 3 Kit storage and stability 3 Suitable sample
More informationoasig TM lyophilised OneStep 2X RT-qPCR Master Mix
Primerdesign TM oasig TM lyophilised OneStep 2X RT-qPCR Master Mix Instructions for use of Primerdesign oasig lyophilised OneStep Master Mix Contents Introduction 3 Kit contents 4 Kit storage 4 Suitable
More informationPrecision MULTIPLEX qpcr Master Mix. Instructions for use of Primerdesign Precision MULTIPLEX Master Mix for real-time PCR
Precision MULTIPLEX qpcr Master Mix Instructions for use of Primerdesign Precision MULTIPLEX Master Mix for real-time PCR Contents Introduction 3 Kit Contents 5 Recommended Accompanying Products 5 Reagents
More informationGenomic DNA quantification assay. Quantification of genomic DNA by real-time PCR
Genomic DNA quantification assay Quantification of genomic DNA by real-time PCR Contents Kit contents 3 Reagents and equipment to be supplied by user 3 Kit storage and stability 4 Suitable sample material
More informationInternal DNA extraction control
Internal DNA extraction control Instructions for use of DNA real-time PCR internal extraction control kit CTRL Internal controls and gdna detection Contents Introduction 3 Kit contents 4 Reagents and equipment
More informationPrecision FAST qpcr Master Mix. Instructions for use of Primerdesign Precision FAST Master Mix for real-time PCR
Precision FAST qpcr Master Mix Instructions for use of Primerdesign Precision FAST Master Mix for real-time PCR Contents Introduction 3 Kit Contents 5 Recommended Accompanying Products 5 Reagents and Equipment
More informationPrecision PLUS qpcr Master Mix. Instructions for use of Primerdesign Precision PLUS Master Mix for real-time PCR
Precision PLUS qpcr Master Mix Instructions for use of Primerdesign Precision PLUS Master Mix for real-time PCR Contents Introduction 3 Kit Contents 5 Recommended accompanying products 5 Reagents and equipment
More informationM. tuberculosis_mpb64/is61 10 genesig Standard Kit
TM Primerdesign Ltd M. tuberculosis_mpb64/is61 10 genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to M.tuberculosis_MPB64/IS6110 2 Specificity MAX MIN The Primerdesign
More informationDetection of Prothrombin (G20210A)
TM Primerdesign Ltd (G20210A) Real-time PCR mutation detection /allelic discrimination kit 50 tests For general laboratory and research use only 1 Kit Contents Prothrombin genotyping primer/probe mix (50
More informationM. tuberculosis_mpb64/is611. genesig Advanced Kit. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd M. tuberculosis_mpb64/is611 0 genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to M.tuberculosis_MPB64/IS6110 2 Specificity MAX MIN The Primerdesign
More informationSus scrofa Pig Speciation Kit
Techne qpcr test Sus scrofa Speciation Kit 100 tests For general laboratory and research use only 1 2 Kit Contents specific primer/probe mix (BROWN) FAM labelled positive control template (RED) Internal
More informationChikungunya. genesig Standard Kit. Non structural protein 2 (nsp2) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Chikungunya Non structural protein 2 (nsp2) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Chikungunya Chikungunya virus (CHIKV) is an
More informationDetection of FactorV Leiden (R506Q)
TM Primerdesign Ltd Detection of FactorV Leiden (R506Q) snpsig TM real-time PCR mutation detection/allelic discrimination kit 50 tests For general laboratory and research use only 1 Kit contents Factor
More informationDengue Virus subtypes 1,2 3 and 4
TM Primerdesign Ltd Dengue Virus subtypes 1,2 3 and 4 3 Untranslated Region (3 UTR) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Dengue Virus subtypes 1,2
More informationCaMV promoter & NOS terminator detection
Primerdesign TM Ltd GMO event quantification CaMV promoter & NOS terminator detection Detection and quantification of GMO integration events in Soya by real-time PCR 100 tests Kit contents CaMV-GM primer/probe
More informationTamiflu resistance detection kit
Tamiflu resistance detection kit H1N1 (H275Y) genesig real-time PCR mutation detection/allelic discrimination kit 150 tests For general laboratory and research use only genesig Tamiflu resistance detection
More informationHuman Parainfluenza Virus type 3 (HPIV-3)
TM Primerdesign Ltd Human Parainfluenza Virus type 3 (HPIV-3) Haemagglutinin-Neuraminidase glycoprotein genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human
More informationDetection of CFTR(F508) MyBioSource.com. Real-time PCR mutation detection/allelic discrimination kit. For general laboratory and research use only
Detection of CFTR(F508) Real-time PCR mutation detection/allelic discrimination kit For general laboratory and research use only 50 tests Contents Principles of the test 3 Kit Contents 5 Recommended Accompanying
More informationBacteriophage MS2. genesig Standard Kit. Phage MS2 genome. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Bacteriophage MS2 Phage MS2 genome genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Bacteriophage MS2 Bacteriophage MS2 is a non-enveloped,
More informationMaize Chlorotic Mottle Virus
TM Primerdesign Ltd Maize Chlorotic Mottle Virus Coat Protein Gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Maize Chlorotic Mottle Virus Maize chlorotic
More informationAvian Infectious Bronchitis Virus (IBV)
TM Primerdesign Ltd Avian Infectious Bronchitis Virus (IBV) ORF1a gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Avian Infectious Bronchitis Virus (IBV)
More informationTechne qpcr test. Pisum sativum. Ribosomal protein S12 (rps12) gene. 100 tests
Techne qpcr test Pisum sativum Ribosomal protein S12 (rps12) gene 100 tests For general laboratory and research use only Introduction to Pisum sativum 1 2 Specificity The Techne Kit for Pisum sativum (P.sativum)
More informationHepatitis A Virus. genesig Standard Kit 5 NCR. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Hepatitis A Virus 5 NCR genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Hepatitis A Virus Hepatitis virus (HAV) is a non-enveloped ssrna
More informationAnas platyrhynchos Duck. Advanced Speciation Kit. 100 tests. For general laboratory and research use only
Anas platyrhynchos Duck Advanced Speciation Kit 100 tests For general laboratory and research use only Carry over contamination between PCR reactions can be prevented by including uracil-nglycosylase
More informationLactobacillus 16S Ribosomal RNA. genesig Advanced Kit. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Lactobacillus 16S Ribosomal RNA genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Lactobacillus The Lactobacillus genus is a group of Gram-positive,
More informationNOS terminator (GMO) genesig Advanced Kit. NOS terminator. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd NOS terminator (GMO) NOS terminator genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to NOS terminator (GMO) The kit provides a method for
More informationDengue Virus subtypes 1, 2, 3 and 4 (Multiplex kit)
Primerdesign TM Ltd Dengue Virus subtypes 1, 2, 3 and 4 (Multiplex kit) genesig advanced kit 100 tests For general laboratory and research use only 1 Contents Introduction to Dengue Virus 3 Specificity
More informationChikungunya. genesig Advanced Kit. Non structural protein 2 (nsp2) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Chikungunya Non structural protein 2 (nsp2) genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Chikungunya Chikungunya virus (CHIKV) is an
More informationFMV 35S promoter (GMO) Advanced Kit. FMV 35S promoter. 150 tests. For general laboratory and research use only
FMV 35S promoter (GMO) FMV 35S promoter 150 tests Advanced Kit For general laboratory and research use only Introduction to FMV 35S promoter (GMO) The kit provides a method for detecting gene insertion
More informationLove Bird (Agapornis) Sexing Sex chromosome specific spindlin gene. Advanced Kit. 150 tests. For general laboratory and research use only
Love Bird (Agapornis) Sexing Sex chromosome specific spindlin gene 150 tests Advanced Kit For general laboratory and research use only Introduction to Love Bird (Agapornis) Sexing Specificity MAX MIN The
More informationEGFR T790M mutation mutation detection by quantitative allele specific amplification (quasa) EGFR (T790M)
Primerdesign TM Ltd EGFR T790M mutation mutation detection by quantitative allele specific amplification (quasa) EGFR (T790M) 50 tests For general laboratory and research use only 1 Contents Introduction
More informationPrecision PLUS OneStep qrt-pcr Master Mix. Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-PCR
Precision PLUS OneStep qrt-pcr Master Mix Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-PCR Contents Introduction 3 Kit Contents 5 Recommended Accompanying Products
More informationMycobacterium Tuberculosis
TM Primerdesign Ltd Mycobacterium Tuberculosis rep13e12 genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis
More informationSus scrofa Pig genesig Advanced Speciation Kit
TM Primerdesign Ltd Pig genesig Advanced Speciation Kit 100 tests For general laboratory and research use only 1 Principles of the test Real-time PCR This kit provides a method for detecting mitochondrial
More informationHuman Parainfluenza Virus type 4b
TM Primerdesign Ltd Human Parainfluenza Virus type 4b Phosphoprotein (P) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Parainfluenza Virus type
More informationHepatitis E virus (HEV)
Techne qpcr test Hepatitis E virus (HEV) Open Reading Frame 2 (ORF2) 150 tests For general laboratory and research use only 1 Introduction to Hepatitis E virus (HEV) The Hepatitis E Virus is a member of
More informationPistacia vera. Introduction to Pistacia vera. 100 tests. Techne qpcr test. For general laboratory and research use only
Techne qpcr test Pistacia vera 100 tests For general laboratory and research use only Introduction to Pistacia vera 1 2 Specificity The Techne Kit for Pistacia vera (P.vera) genomes is designed for the
More informationHuman T-lymphotropic Virus 1
TM Primerdesign Ltd Human T-lymphotropic Virus 1 Polymerase gene (POL) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human T-lymphotropic Virus 1 Human T-lymphotropic
More informationEquus caballus Horse Speciation Kit
PCRmax Ltd TM qpcr test Equus caballus Speciation Kit 100 tests For general laboratory and research use only 1 Principles of the test Real-time PCR This kit provides a method for detecting Equus caballus
More informationSaccharomyces cerevisiae
TM Primerdesign Ltd Saccharomyces cerevisiae Actin (ACT1) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Saccharomyces cerevisiae Saccharomyces cerevisiae
More informationMaize CaMV promoter & NOS terminator (GMO)
PCRmax Ltd TM qpcr test Maize CaMV promoter & NOS terminator (GMO) Maize CaMV promoter & NOS terminator 150 tests For general laboratory and research use only 1 Introduction to Maize CaMV promoter & NOS
More informationFoot & Mouth Disease Virus
TM Primerdesign Ltd Foot & Mouth Disease Virus 5' untranslated region (5'UTR) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Foot & Mouth Disease Virus Foot-and-mouth
More informationTechne qpcr test. A graveolens. NADPH-dependent mannose 6- phosphate reductase (m6pr) 100 tests
Techne qpcr test A graveolens NADPH-dependent mannose 6- phosphate reductase (m6pr) 100 tests For general laboratory and research use only Introduction to A graveolens 1 2 Specificity The Techne Kit for
More informationHuman Papillomavirus 16
TM Primerdesign Ltd Human Papillomavirus 16 E6 gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Papillomavirus 16 Papillomaviruses are a diverse
More informationHelicobacter pylori. genesig Standard Kit. RNA polymerase beta-subunit (rpob) gene. 150 tests. Primerdesign Ltd
TM Primerdesign Ltd Helicobacter pylori RNA polymerase beta-subunit (rpob) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Helicobacter pylori Helicobacter
More informationDengue Virus subtypes 1,2 3 and 4
TM Primerdesign Ltd Dengue Virus subtypes 1,2 3 and 4 3 Untranslated Region (3 UTR) genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Dengue Virus subtypes 1,2
More informationJAK2 V617F mutation mutation detection by quantitative allele specific amplification (quasa) JAK2(V617F)
Primerdesign TM Ltd JAK2 V617F mutation mutation detection by quantitative allele specific amplification (quasa) JAK2(V617F) 50 tests For general laboratory and research use only 1 Contents Introduction
More informationHuman Herpes Virus 1 (Herpes simplex type 1)
TM Primerdesign Ltd Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human
More informationPrecision PLUS OneStep RT-qPCR Master Mix. Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-qPCR
Precision PLUS OneStep RT-qPCR Master Mix Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-qPCR Contents Introduction 3 Kit contents 5 Recommended accompanying products
More informationMycobacterium Tuberculosis
TM Primerdesign Ltd Mycobacterium Tuberculosis rep13e12 genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Mycobacterium Tuberculosis Mycobacterium tuberculosis
More informationStaphylococcus epidermidis
TM Primerdesign Ltd Staphylococcus epidermidis superoxide dismutase (soda) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Staphylococcus epidermidis
More informationTransmissible gastroenteritis virus
TM Primerdesign Ltd Transmissible gastroenteritis virus Spike Protein (S) Gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Transmissible gastroenteritis
More informationAspergillus all pathogenic subtypes
TM Primerdesign Ltd Aspergillus all pathogenic subtypes 18S ribosomal RNA gene, transcribed spacer 1, 5.8S ribosomal RNA gene genesig Standard Kit 150 tests For general laboratory and research use only
More informationTilapia lake virus. genesig Standard Kit. Hypothetical protein gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Tilapia lake virus Hypothetical protein gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Tilapia lake virus Since 2009 Tilapia lake
More informationTransmissible Gastro Enteritis Virus & Porcine Respiratory Coronavirus
TM Primerdesign Ltd Transmissible Gastro Enteritis Virus & Porcine Respiratory Coronavirus Spike Glycoprotein (S) Gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction
More informationHuman T-lymphotropic Virus 2
TM Primerdesign Ltd Human T-lymphotropic Virus 2 Polymerase gene (POL) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human T-lymphotropic Virus 2 Human T-lymphotropic
More informationEpstein Barr Virus (Human Herpes virus 4)
TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to
More informationBRAF V600E mutation mutation detection by quantitative allele specific amplification (quasa) BRAF (V600E)
Primerdesign TM Ltd BRAF V600E mutation mutation detection by quantitative allele specific amplification (quasa) BRAF (V600E) 50 tests For general laboratory and research use only 1 Contents Introduction
More informationAntibiotic resistance VanA
TM Primerdesign Ltd Antibiotic resistance VanA Vancomycin resistance protein A (vana) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Antibiotic resistance
More informationEscherichia coli_o104: H4
PCRmax Ltd TM qpcr test Escherichia coli_o104: H4 150 tests For general laboratory and research use only 1 Introduction to Escherichia coli_o104:h4 2 Specificity MAX MIN The PCR Max qpcr Kit for Escherichia
More informationHuman Herpes Virus 7 genomes
TM Primerdesign Ltd Human Herpes Virus 7 genomes Glycoprotein H gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Herpes Virus 7 genomes Human Herpesvirus
More informationHuman Rhinovirus all subtypes (generic)
TM Primerdesign Ltd Human Rhinovirus all subtypes (generic) 5 non coding region (5 NCR) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Rhinovirus all
More informationHuman bocavirus. genesig Standard Kit. Viral protein (VP) gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Human bocavirus Viral protein (VP) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human bocavirus Human Bocavirus is a genus of the
More informationPorcine parvovirus. genesig Standard Kit. Structural Protein 2 (VP2) Gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Porcine parvovirus Structural Protein 2 (VP2) Gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Porcine parvovirus Porcine parvovirus
More informationFeline Immunodeficiency virus
TM Primerdesign Ltd Feline Immunodeficiency virus gag protein (gag) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Feline Immunodeficiency virus Feline
More informationRespiratory Syncytial Virus type A (RSV-A)
TM Primerdesign Ltd Respiratory Syncytial Virus type A (RSV-A) Nucleocapsid gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Respiratory Syncytial Virus
More informationArachis hypogaea A.hypogaea. genesig Allergen detection Kit. 100 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Arachis hypogaea A.hypogaea genesig Allergen detection Kit 100 tests For general laboratory and research use only 1 Introduction to Arachis hypogaea Peanut (Arachis hypogaea) belongs
More informationSt. Louis encephalitis virus
TM Primerdesign Ltd St. Louis encephalitis virus NS5 protein (NS5) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to St. Louis encephalitis virus Saint
More informationSoya Round up Ready (RR)
Primerdesign TM Ltd GMO event quantification Soya Round up Ready (RR) Detection and quantification of GMO integration events by real-time PCR 100 tests Kit contents Soya-WT DNA primer/probe mix (100 reactions
More informationSus scrofa Pig genesig Speciation Kit
TM Primerdesign Ltd Sus scrofa genesig Speciation Kit 100 tests For general laboratory and research use only 1 Principles of the test Real-time PCR This kit provides a method for detecting Sus scrofa mitochondrial
More informationEchinococcus granulosus
TM Primerdesign Ltd Echinococcus granulosus NADH dehydrogenase subunit 1 gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Echinococcus granulosus Echinococcus
More informationAcinetobacter baumannii
TM Primerdesign Ltd Acinetobacter baumannii hypothetical protein sequence gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Acinetobacter baumannii Acinetobacter
More informationViral Hemorrhagic Septicemia Virus
TM Primerdesign Ltd Viral Hemorrhagic Septicemia Virus Non-coding region genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Viral Hemorrhagic Septicemia Virus
More informationCryptococcus neoformans
TM Primerdesign Ltd Cryptococcus neoformans Hypothetical protein genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Cryptococcus neoformans Cryptococcus neoformans
More informationSalmon gill poxvirus. genesig Standard Kit. Hypothetical Protein Gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Salmon gill poxvirus Hypothetical Protein Gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Salmon gill poxvirus Poxviridae are a family
More informationHuman Parainfluenza Virus type 3 (HPIV-3)
Techne qpcr test Human Parainfluenza Virus type 3 (HPIV-3) Haemagglutinin-Neuraminidase glycoprotein 150 tests For general laboratory and research use only 1 Introduction to Human Parainfluenza Virus type
More informationPrunus dulcis genesig Allergen detection Kit
TM Primerdesign Ltd TM Primerdesign Ltd Prunus dulcis genesig Allergen detection Kit 100 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research use only 1 Introduction
More informationCorylus avellana C.avellana. genesig Allergen detection Kit. 100 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Corylus avellana C.avellana genesig Allergen detection Kit 100 tests For general laboratory and research use only 1 Introduction to Corylus avellana Hazelnut - The Common Hazel (Corylus
More informationAntibiotic resistance OXA -48 Carbapenem-hydrolyzing β- lactamase (blaoxa-48) gene. genesig Advanced Kit. 150 tests.
TM Primerdesign Ltd Antibiotic resistance OXA -48 Carbapenem-hydrolyzing β- lactamase (blaoxa-48) gene genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Antibiotic
More informationMyBioSource.com. Hepatitis E virus (HEV) Advanced Kit. ORF2 capsid protein gene. 150 tests. For general laboratory and research use only
Hepatitis E virus (HEV) ORF2 capsid protein gene 150 tests Advanced Kit For general laboratory and research use only Introduction to Hepatitis E virus (HEV) The Hepatitis E Virus is a member of the Hepeviridae
More informationPrecision PLUS OneStep RT-qPCR Master Mix. Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-qPCR
Precision PLUS OneStep RT-qPCR Master Mix Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-qPCR Contents Introduction 3 Kit contents 5 Recommended accompanying products
More informationMycoplasma bovis. genesig Standard Kit. DNA gyrase subunit B (gyrb) gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Mycoplasma bovis DNA gyrase subunit B (gyrb) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Mycoplasma bovis Mycoplasmas are small,
More informationHuman Papillomavirus 16
TM Primerdesign Ltd Human Papillomavirus 16 E6 gene genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Human Papillomavirus 16 Papillomaviruses are a diverse
More informationHuman Herpes Virus 1 (Herpes simplex type 1)
TM Primerdesign Ltd Human Herpes Virus 1 (Herpes simplex type 1) Capsid assembly and DNA maturation gene genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Human
More informationPrunus dulcis P.dulcis. genesig Allergen detection Kit. 100 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Prunus dulcis P.dulcis genesig Allergen detection Kit 100 tests For general laboratory and research use only 1 Introduction to Prunus dulcis Almond (Prunus dulcis) is the seed from
More informationStaphylococcus epidermidis
TM Primerdesign Ltd Staphylococcus epidermidis superoxide dismutase (soda) gene genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Staphylococcus epidermidis
More informationMycobacterium avium. genesig Standard Kit. Transposon element (IS1245) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Mycobacterium avium Transposon element (IS1245) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Mycobacterium avium Mycobacterium avium
More informationEpstein Barr Virus (Human Herpes virus 4)
TM Primerdesign Ltd Epstein Barr Virus (Human Herpes virus 4) nonglycosylated membrane protein (BNRF1) gene genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to
More informationCandida dubliniensis. genesig Standard Kit. Cytosine deaminase (FCA1) gene. 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Candida dubliniensis Cytosine deaminase (FCA1) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Candida dubliniensis Candida dubliniensis
More information